The green innovation value chain is a key step in transforming green,innovative scientific,and technological achievements into productive forces.The establishment of green innovation value chains based on value distri...The green innovation value chain is a key step in transforming green,innovative scientific,and technological achievements into productive forces.The establishment of green innovation value chains based on value distribution rather than technical conditions can effectively overcome the common bottleneck faced by different nations during their green innovation endeavors,namely,the substitution of conventional products with green alternatives.This study investigates the impeded diffusion of green products and their underlying causes,analyzes the internal structure and mechanism of the green innovation value chain,and explores the establishment of regional green innovation value chains and the models available for value chain upgrading.展开更多
为了建立水禽细小病毒(WPV)快速检测方法,根据序列比对结果在水禽细小病毒NS基因SF3保守区域内设计特异性引物,建立SYBR Green Ⅰ荧光定量PCR通用检测方法。该方法的扩增效率(E)为90.0%,相关系数(R~2)=0.99,标准曲线方程为y=-3.607x+38....为了建立水禽细小病毒(WPV)快速检测方法,根据序列比对结果在水禽细小病毒NS基因SF3保守区域内设计特异性引物,建立SYBR Green Ⅰ荧光定量PCR通用检测方法。该方法的扩增效率(E)为90.0%,相关系数(R~2)=0.99,标准曲线方程为y=-3.607x+38.77;除WPV出现S形扩增曲线外,新城疫病毒(NDV)、H9亚型禽流感病毒(H9 AIV)、鸭坦布苏病毒(DTMUV)、鸭肝炎病毒(DHAV)、鸭肠炎病毒(DEV)、鸭呼肠孤病毒(DRV)样品均未出现S形阳性扩增曲线;批内变异系数(CV)为0.15%~0.23%,批间变异系数为0.09%~0.28%。结果表明,SYBR Green Ⅰ荧光定量PCR检测方法重复性好、灵敏度高和特异性强。临床样品检测结果表明,SYBR Green Ⅰ荧光定量PCR与普通PCR的符合率达98.4%,灵敏度是普通PCR的1 000倍。SYBR Green Ⅰ荧光定量PCR检测方法不仅能定性检测WPV,还可以进行定量检测,可用于种鸭场、种鹅场的WPV净化检测,也可用于WPV临床大量样品的快速检测。展开更多
Neuronal injuries such as stroke,traumatic brain injury,and spinal cord injury are leading causes of major disability and death.Chronic therapy for these neuronal injuries requires the promotion of axonal regeneration...Neuronal injuries such as stroke,traumatic brain injury,and spinal cord injury are leading causes of major disability and death.Chronic therapy for these neuronal injuries requires the promotion of axonal regeneration from the remaining neurons（Schwab and Strittmatter,2014）.展开更多
为了建立高效、灵敏的猪流行性腹泻病毒(PEDV)检测方法,本研究从GenBank数据库中获取PEDV N基因序列,扩增出PEDV N基因标准质粒,并在N基因的保守区域内设计了一对特异性荧光定量引物,成功建立了SYBR Green I实时荧光定量PCR检测方法。...为了建立高效、灵敏的猪流行性腹泻病毒(PEDV)检测方法,本研究从GenBank数据库中获取PEDV N基因序列,扩增出PEDV N基因标准质粒,并在N基因的保守区域内设计了一对特异性荧光定量引物,成功建立了SYBR Green I实时荧光定量PCR检测方法。经过一系列试验表明,该检测方法线性关系良好,R^(2)值为0.99;特异性强,敏感性高,最低可检测至2.23 copies/μL,比普通PCR灵敏约100倍;重复性好,组内变异系数为0.25%~0.43%,组间变异系数为0.67%~0.97%;对于各地区96份临床样品检测出PEDV阳性率为25%。本研究建立的实时荧光定量PCR检测方法为PEDV的临床诊断、流行病学调查以及定量研究提供了有效的检测工具。展开更多
Brand building of agricultural products is the key for agricultural enterprises to gain competitive advantage and occupy the market. Guizhou province is rich in green agricultural products resources and has superior n...Brand building of agricultural products is the key for agricultural enterprises to gain competitive advantage and occupy the market. Guizhou province is rich in green agricultural products resources and has superior natural resources, but it is at a disadvantage in the market competition. The constraint factor is that the brand is not enough to obtain consumer trust. In order to provide countermeasures and suggestions for Guizhou provincial government and agricultural enterprises to shape green agricultural products brand, this paper explores the influence path of country-of-origin image of Guizhou province on brand trust of green agricultural products. Therefore, based on the theory of Country-of-origin effects this study conducts an empirical research. Taking the country-of-origin image, including natural conditions,technological conditions, brand supervision norms, technology input, government marketing promotion and government policies as antecedent variables, and green agricultural brand trust, including brand competence trust and brand quality trust as dependent variables, the structural equation model was constructed. Through data collection and empirical analysis by using structural equation model, this study found that natural conditions have a positive impact on brand competence trust, and technical conditions have a positive impact on brand quality trust. The brand supervision norms, technology input, government marketing promotion and government policy will all promote consumers to have a positive impact on brand trust in green agricultural products.展开更多
The paper builds up a cost-benefit measuring model of green products in manufacturing industry throughout its full life cycle, which can quantify green products＇ cost and benefit completely and correctly under the ci...The paper builds up a cost-benefit measuring model of green products in manufacturing industry throughout its full life cycle, which can quantify green products＇ cost and benefit completely and correctly under the circumstance of satisfying enterprise, customer, environment and society. It also puts forth an operable method to estimate social benefit by opportunity cost and establishes a profit maximization-programming model. The model can be applied to justify whether some kinds of green products should be developed and produced.展开更多
The appraisement of "the green level" for a green product is a problem that has to deal with multi aspects and multi stratification. According to the property of the items that affect "the green level...The appraisement of "the green level" for a green product is a problem that has to deal with multi aspects and multi stratification. According to the property of the items that affect "the green level" of the product, they can be divided into aspects, elements and factors. These make appreciable grades. Multi level fuzzy judgement can solve the appraisement problem that considers the influences of design, manufacture, use and recovery in all life cycle or multi life cycle of a product. In the judging process it not only deals with the action of all factors, but also continues to have all messages in grade judgement. The power coefficients stand out as the main items. An example is given to show the judgement process.展开更多
The use of genetically modified organisms （GMOs） as food products becomes more and more widespread. The European Union has implemented a set of very strict procedures for the approval to grow, import and/or utilize ...The use of genetically modified organisms （GMOs） as food products becomes more and more widespread. The European Union has implemented a set of very strict procedures for the approval to grow, import and/or utilize GMOs as food or food ingredients. Thus, analytical methods for detection of GMOs are necessary in order to verify compliance with labelling requirements. There are few effective screening methods for processed GM （genetically modified） products. Three anti-herbicide genes （CP4- EPSPS, BAR and PAT） are common exogenous genes used in commercialized transgenic soybean, maize and rice, In the present study, a new SYBR Green qPCR screening method was developed to simultaneously detect the three exogenous anti-herbicide genes and one endogenous gene in a run. We tested seven samples of representative processed products （soya lecithin, soya protein powder, chocolate beverage, infant rice cereal, maize protein powder, maize starch, and maize jam） using the developed method, and amplicons of endogenous gene and transgenic fragments were obtained from all the processed products, and the sensitivity was 0.1%. These results indicated that SYBR Green qPCR screening method was appropriate for qualitative detection of transgenic soybean, maize and rice in processed products.展开更多
Maillard Reaction Products (MRP) was prepared from glucose and glysine heated at 90°C in air circulating oven for 12 hours. The effect of MRP on chlorophyll stability in green peas was investigated. The addition ...Maillard Reaction Products (MRP) was prepared from glucose and glysine heated at 90°C in air circulating oven for 12 hours. The effect of MRP on chlorophyll stability in green peas was investigated. The addition of Maillard Reaction Products (MRP) decreased the loss of chlorophyll a, chlorophyll b and total chlorophyll. The conversion of pheophytin and pyropheophytin were also reduced by the addition of MRP.展开更多
Paper products such as corrugated paperboards are the most common green packaging materials, which are renewable, sustainable, recyclable and biodegradable. However, the plastic or metal straps used to secure the cart...Paper products such as corrugated paperboards are the most common green packaging materials, which are renewable, sustainable, recyclable and biodegradable. However, the plastic or metal straps used to secure the carton boxes are not so green. At the end of packaging, the carton boxes can be recycled, but the plastic/metal straps have to be sorted out for disposal separately. This review focuses on: 1) the global trend of green packaging;2) conventional plastic/metal strapping materials for carton boxes;3) conventional market pulp baling with steel wire as the tying materials;4) cellulose fiber-based materials for strapping market pulp bales and carton boxes. New generation of cellulose paper straps are being developed for more challenging applications with superior strength properties and repulpability.展开更多
The appraisement of "the green level" for a green p ro duct is a problem that has to deal with multi-aspect and multi-stratification. According to the property of the items that affect "the green level&...The appraisement of "the green level" for a green p ro duct is a problem that has to deal with multi-aspect and multi-stratification. According to the property of the items that affect "the green level" of the pro duct, they can be divided into aspects, elements and factors. These make appreci able grades. The multi-level fuzzy judgement can solve the appraisement problem that considers the influences of design, manufacture, use and recovery in all- life cycle or multi-life cycle of product. In the judging process it is not onl y deal with the action of all factors, but also continue to have all messages in grade judgement. The power coefficients stand out as the main items. An example is given to show the judgement process.展开更多
The paper introduces the lemon effect of green agricultural products,analyzes the formation reasons of the lemon effect of green agricul-tural products and summarizes problems brought by the effect,such as malicious d...The paper introduces the lemon effect of green agricultural products,analyzes the formation reasons of the lemon effect of green agricul-tural products and summarizes problems brought by the effect,such as malicious deception and high price.The paper proposes countermeasures toavoid the lemon effect of green agricultural products from a perspective of marketing.The first is to strengthen the quality supervision of green agri-cultural products,upgrade the quality of products,and build up branded products.The government should foster the main body of the products andguide the main body to realize the importance of brand construction and management.The second is to construct a sales channel system of greenagricultural products,making use of the trading center of modern green agricultural products to sell products,developing a long term partnershipwith processing industries,big supermarket and restaurants,making use of internet and selling products online and offline.The third is to propagatethe products.Make a good use of advertisement,personal sales,propagation and public relations to accelerate the healthy development of greenagricultural market.展开更多
With the worsening of environment, many countries pay more attention to the environmental protection. Green （organic） trade barrier gradually attracts more attention to the non-tariff barrier in the international tr...With the worsening of environment, many countries pay more attention to the environmental protection. Green （organic） trade barrier gradually attracts more attention to the non-tariff barrier in the international trade. However, according to the economic levels of different countries, great differences exist in environment standards of these countries which resulted in the appearance of green trade barriers as trade protection. This study classified the connotations and characteristics of green trade barrier, gave the descriptions on the development and type of green trade barrier, and analysed the influences on China＇s export and put forward some countermeasures which aimed at stimulating the development of our foreign trade.展开更多
Based on the overview and characteristics of agricultural products processing enterprise in Yinchuan City,green marketing status of agricultural products processing enterprise in Yinchuan City is introduced from the a...Based on the overview and characteristics of agricultural products processing enterprise in Yinchuan City,green marketing status of agricultural products processing enterprise in Yinchuan City is introduced from the aspects of product strategy,pricing strategy,distribution strategy and promotion strategy.Problems in the green marketing of agricultural products processing enterprise are analyzed,such as the obvious contradiction between the processing demand and the raw materials,low level of comprehensive utilization of resources,no common demand for green consumption in Yinchuan City,weak strength of enterprise and no concept of green marketing,poor macro-control and insufficient input,neglecting the environmental production of materials during marketing,and insufficient input of enterprises in professional marketing personnel.In order to improve the green marketing ability of enterprises and the development of agricultural products processing enterprise in Yinchuan City,the following countermeasures are put forward:green marketing strategy(mainly including cultivating the green marketing strategy for enterprises,gathering of green information,and strengthening the marketing strategy of target market)and green marketing policy(mainly including green products policy,green price policy,green channel policy and green promotion policy).展开更多
基金the part of the“Research on Paths to High-quality Development in Agriculture Against the Backdrop of Rural Revitalization,”a project of the Publicity Department of the CPC Central Committee for Young Talents in Publicity,Socialist Thought and Cultural Promotionthe National Social Science Fund of China-supported project,“Research on Theoretical Logic and Realization Path of Urban-Rural Integration Based on Industrial Internet”(21XJL001)+1 种基金the major project of Sichuan province in philosophy and social science planning,“Research on the Innovation and Policy Adaptation of Sichuan’s Agricultural Green Development System under the‘Dual Carbon’Goal”(SC22ZDYC44)the key project of Sichuan province in soft science research and planning,“Research on the Path to Peak Carbon and Carbon Neutrality of the Agricultural Sector in Rural Areas of Sichuan Province”(2022JDR0157).
文摘The green innovation value chain is a key step in transforming green,innovative scientific,and technological achievements into productive forces.The establishment of green innovation value chains based on value distribution rather than technical conditions can effectively overcome the common bottleneck faced by different nations during their green innovation endeavors,namely,the substitution of conventional products with green alternatives.This study investigates the impeded diffusion of green products and their underlying causes,analyzes the internal structure and mechanism of the green innovation value chain,and explores the establishment of regional green innovation value chains and the models available for value chain upgrading.
文摘为了建立水禽细小病毒(WPV)快速检测方法,根据序列比对结果在水禽细小病毒NS基因SF3保守区域内设计特异性引物,建立SYBR Green Ⅰ荧光定量PCR通用检测方法。该方法的扩增效率(E)为90.0%,相关系数(R~2)=0.99,标准曲线方程为y=-3.607x+38.77;除WPV出现S形扩增曲线外,新城疫病毒(NDV)、H9亚型禽流感病毒(H9 AIV)、鸭坦布苏病毒(DTMUV)、鸭肝炎病毒(DHAV)、鸭肠炎病毒(DEV)、鸭呼肠孤病毒(DRV)样品均未出现S形阳性扩增曲线;批内变异系数(CV)为0.15%~0.23%,批间变异系数为0.09%~0.28%。结果表明,SYBR Green Ⅰ荧光定量PCR检测方法重复性好、灵敏度高和特异性强。临床样品检测结果表明,SYBR Green Ⅰ荧光定量PCR与普通PCR的符合率达98.4%,灵敏度是普通PCR的1 000倍。SYBR Green Ⅰ荧光定量PCR检测方法不仅能定性检测WPV,还可以进行定量检测,可用于种鸭场、种鹅场的WPV净化检测,也可用于WPV临床大量样品的快速检测。
文摘Neuronal injuries such as stroke,traumatic brain injury,and spinal cord injury are leading causes of major disability and death.Chronic therapy for these neuronal injuries requires the promotion of axonal regeneration from the remaining neurons（Schwab and Strittmatter,2014）.
文摘Brand building of agricultural products is the key for agricultural enterprises to gain competitive advantage and occupy the market. Guizhou province is rich in green agricultural products resources and has superior natural resources, but it is at a disadvantage in the market competition. The constraint factor is that the brand is not enough to obtain consumer trust. In order to provide countermeasures and suggestions for Guizhou provincial government and agricultural enterprises to shape green agricultural products brand, this paper explores the influence path of country-of-origin image of Guizhou province on brand trust of green agricultural products. Therefore, based on the theory of Country-of-origin effects this study conducts an empirical research. Taking the country-of-origin image, including natural conditions,technological conditions, brand supervision norms, technology input, government marketing promotion and government policies as antecedent variables, and green agricultural brand trust, including brand competence trust and brand quality trust as dependent variables, the structural equation model was constructed. Through data collection and empirical analysis by using structural equation model, this study found that natural conditions have a positive impact on brand competence trust, and technical conditions have a positive impact on brand quality trust. The brand supervision norms, technology input, government marketing promotion and government policy will all promote consumers to have a positive impact on brand trust in green agricultural products.
基金This paper is supported by National Nature Science Foundation of China （No.70472034）.
文摘The paper builds up a cost-benefit measuring model of green products in manufacturing industry throughout its full life cycle, which can quantify green products＇ cost and benefit completely and correctly under the circumstance of satisfying enterprise, customer, environment and society. It also puts forth an operable method to estimate social benefit by opportunity cost and establishes a profit maximization-programming model. The model can be applied to justify whether some kinds of green products should be developed and produced.
文摘The appraisement of "the green level" for a green product is a problem that has to deal with multi aspects and multi stratification. According to the property of the items that affect "the green level" of the product, they can be divided into aspects, elements and factors. These make appreciable grades. Multi level fuzzy judgement can solve the appraisement problem that considers the influences of design, manufacture, use and recovery in all life cycle or multi life cycle of a product. In the judging process it not only deals with the action of all factors, but also continues to have all messages in grade judgement. The power coefficients stand out as the main items. An example is given to show the judgement process.
基金Supported by the Funds of the Scientific Research Foundation of Northeast Agricultural University(2010RCB53)
文摘The use of genetically modified organisms （GMOs） as food products becomes more and more widespread. The European Union has implemented a set of very strict procedures for the approval to grow, import and/or utilize GMOs as food or food ingredients. Thus, analytical methods for detection of GMOs are necessary in order to verify compliance with labelling requirements. There are few effective screening methods for processed GM （genetically modified） products. Three anti-herbicide genes （CP4- EPSPS, BAR and PAT） are common exogenous genes used in commercialized transgenic soybean, maize and rice, In the present study, a new SYBR Green qPCR screening method was developed to simultaneously detect the three exogenous anti-herbicide genes and one endogenous gene in a run. We tested seven samples of representative processed products （soya lecithin, soya protein powder, chocolate beverage, infant rice cereal, maize protein powder, maize starch, and maize jam） using the developed method, and amplicons of endogenous gene and transgenic fragments were obtained from all the processed products, and the sensitivity was 0.1%. These results indicated that SYBR Green qPCR screening method was appropriate for qualitative detection of transgenic soybean, maize and rice in processed products.
文摘Maillard Reaction Products (MRP) was prepared from glucose and glysine heated at 90°C in air circulating oven for 12 hours. The effect of MRP on chlorophyll stability in green peas was investigated. The addition of Maillard Reaction Products (MRP) decreased the loss of chlorophyll a, chlorophyll b and total chlorophyll. The conversion of pheophytin and pyropheophytin were also reduced by the addition of MRP.
文摘Paper products such as corrugated paperboards are the most common green packaging materials, which are renewable, sustainable, recyclable and biodegradable. However, the plastic or metal straps used to secure the carton boxes are not so green. At the end of packaging, the carton boxes can be recycled, but the plastic/metal straps have to be sorted out for disposal separately. This review focuses on: 1) the global trend of green packaging;2) conventional plastic/metal strapping materials for carton boxes;3) conventional market pulp baling with steel wire as the tying materials;4) cellulose fiber-based materials for strapping market pulp bales and carton boxes. New generation of cellulose paper straps are being developed for more challenging applications with superior strength properties and repulpability.
文摘The appraisement of "the green level" for a green p ro duct is a problem that has to deal with multi-aspect and multi-stratification. According to the property of the items that affect "the green level" of the pro duct, they can be divided into aspects, elements and factors. These make appreci able grades. The multi-level fuzzy judgement can solve the appraisement problem that considers the influences of design, manufacture, use and recovery in all- life cycle or multi-life cycle of product. In the judging process it is not onl y deal with the action of all factors, but also continue to have all messages in grade judgement. The power coefficients stand out as the main items. An example is given to show the judgement process.
文摘The paper introduces the lemon effect of green agricultural products,analyzes the formation reasons of the lemon effect of green agricul-tural products and summarizes problems brought by the effect,such as malicious deception and high price.The paper proposes countermeasures toavoid the lemon effect of green agricultural products from a perspective of marketing.The first is to strengthen the quality supervision of green agri-cultural products,upgrade the quality of products,and build up branded products.The government should foster the main body of the products andguide the main body to realize the importance of brand construction and management.The second is to construct a sales channel system of greenagricultural products,making use of the trading center of modern green agricultural products to sell products,developing a long term partnershipwith processing industries,big supermarket and restaurants,making use of internet and selling products online and offline.The third is to propagatethe products.Make a good use of advertisement,personal sales,propagation and public relations to accelerate the healthy development of greenagricultural market.
基金Heilongjiang Province Humanity Social Science F (10552015)
文摘With the worsening of environment, many countries pay more attention to the environmental protection. Green （organic） trade barrier gradually attracts more attention to the non-tariff barrier in the international trade. However, according to the economic levels of different countries, great differences exist in environment standards of these countries which resulted in the appearance of green trade barriers as trade protection. This study classified the connotations and characteristics of green trade barrier, gave the descriptions on the development and type of green trade barrier, and analysed the influences on China＇s export and put forward some countermeasures which aimed at stimulating the development of our foreign trade.
基金Supported by the Science Research Foundation of Ningxia University((E)NDSK09-36)
文摘Based on the overview and characteristics of agricultural products processing enterprise in Yinchuan City,green marketing status of agricultural products processing enterprise in Yinchuan City is introduced from the aspects of product strategy,pricing strategy,distribution strategy and promotion strategy.Problems in the green marketing of agricultural products processing enterprise are analyzed,such as the obvious contradiction between the processing demand and the raw materials,low level of comprehensive utilization of resources,no common demand for green consumption in Yinchuan City,weak strength of enterprise and no concept of green marketing,poor macro-control and insufficient input,neglecting the environmental production of materials during marketing,and insufficient input of enterprises in professional marketing personnel.In order to improve the green marketing ability of enterprises and the development of agricultural products processing enterprise in Yinchuan City,the following countermeasures are put forward:green marketing strategy(mainly including cultivating the green marketing strategy for enterprises,gathering of green information,and strengthening the marketing strategy of target market)and green marketing policy(mainly including green products policy,green price policy,green channel policy and green promotion policy).
