Bone marrow-derived mesenchymal stem cell-derived exosomeloaded miR-129-5p targets high-mobility group box 1 attenuates neurological-impairment after diabetic cerebral hemorrhage

BACKGROUND Diabetic intracerebral hemorrhage(ICH)is a serious complication of diabetes.The role and mechanism of bone marrow mesenchymal stem cell(BMSC)-derived exosomes(BMSC-exo)in neuroinflammation post-ICH in patients with diabetes are unknown.In this study,we investigated the regulation of BMSC-exo on hyperglycemia-induced neuroinflammation.AIM To study the mechanism of BMSC-exo on nerve function damage after diabetes complicated with cerebral hemorrhage.METHODS BMSC-exo were isolated from mouse BMSC media.This was followed by transfection with microRNA-129-5p(miR-129-5p).BMSC-exo or miR-129-5poverexpressing BMSC-exo were intravitreally injected into a diabetes mouse model with ICH for in vivo analyses and were cocultured with high glucoseaffected BV2 cells for in vitro analyses.The dual luciferase test and RNA immunoprecipitation test verified the targeted binding relationship between miR-129-5p and high-mobility group box 1(HMGB1).Quantitative polymerase chain reaction,western blotting,and enzyme-linked immunosorbent assay were conducted to assess the levels of some inflammation factors,such as HMGB1,interleukin 6,interleukin 1β,toll-like receptor 4,and tumor necrosis factorα.Brain water content,neural function deficit score,and Evans blue were used to measure the neural function of mice.RESULTS Our findings indicated that BMSC-exo can promote neuroinflammation and functional recovery.MicroRNA chip analysis of BMSC-exo identified miR-129-5p as the specific microRNA with a protective role in neuroinflammation.Overexpression of miR-129-5p in BMSC-exo reduced the inflammatory response and neurological impairment in comorbid diabetes and ICH cases.Furthermore,we found that miR-129-5p had a targeted binding relationship with HMGB1 mRNA.CONCLUSION We demonstrated that BMSC-exo can reduce the inflammatory response after ICH with diabetes,thereby improving the neurological function of the brain.

Enhanced and controllable reflected group delay based on Tamm surface plasmons with Dirac semimetals

The reflected group delay from a multilayer structure comprising a one-dimensional photonic crystal coated with a bulk Dirac semimetal(BDS)separated by a spacer layer is investigated theoretically.It is shown that the group delay of the reflected beam in this structure can be significantly negatively enhanced and switched from negative to positive.The enhanced group delay originates from the steep phase change caused by the excitation of the optical Tamm state at the interface between the BDS and spacer layer.Moreover,positive and negative group delays can be actively tuned through the Fermi energy and the relaxation time of the BDS.We believe that this enhanced and tunable delay scheme has important research significance for the fabrication of optical delay devices.

Enhancement of the group delay in quadratic coupling optomechanical systems subjected to an external force

We theoretically study the effect of the quadratic coupling strength on optomechanical systems subjected to a continuous external force. Quadratic coupling strength originates from strong coupling between the optical and the mechanical degrees of freedom. We show that the quadratic coupling strength reduces the amplitude of the dispersion spectra at the resonance in both blue-and red-sideband regimes. However, it increases(decreases) the amplitude of the absorption spectrum in the blue-(red-)sideband regime. Furthermore, in both sideband regimes, the effective detuning between the pump and the cavity deviates with the quadratic coupling strength. Thereby, appropriate selection of the quadratic coupling strength results in an important magnification(in absolute value) of the group delay for both slow and fast light exiting from the optomechanical cavity.

MiR-142-3p Regulates ILC1s by Targeting HMGB1 via the NF-κB Pathway in a Mouse Model of Early Pregnancy Loss

Objective Innate lymphoid cells(ILCs)are a class of newly discovered immunocytes.Group 1 ILCs(ILC1s)are identified in the decidua of humans and mice.High mobility group box 1(HMGB1)is predicted to be one of the target genes of miR-142-3p,which is closely related to pregnancy-related diseases.Furthermore,miR-142-3p and HMGB1 are involved in regulating the NF-κB signaling pathway.This study aimed to examine the regulatory effect of miR-142-3p on ILC1s and the underlying mechanism involving HMGB1 and the NF-κB signaling pathway.Methods Mouse models of normal pregnancy and abortion were constructed,and the alterations of ILC1s,miR-142-3p,ILC1 transcription factor(T-bet),and pro-inflammatory cytokines of ILC1s(TNF-α,IFN-γand IL-2)were detected in mice from different groups.The targeting regulation of HMGB1 by miR-142-3p in ILC1s,and the expression of HMGB1 in normal pregnant mice and abortive mice were investigated.In addition,the regulatory effects of miR-142-3p and HMGB1 on ILC1s were detected in vitro by CCK-8,Annexin-V/PI,ELISA,and RT-PCR,respectively.Furthermore,changes of the NF-κB signaling pathway in ILC1s were examined in the different groups.For the in vivo studies,miR-142-3p-Agomir was injected in the uterus of abortive mice to evaluate the abortion rate and alterations of ILC1s at the maternal-fetal interface,and further detect the expression of HMGB1,pro-inflammatory cytokines,and the NF-κB signaling pathway.Results The number of ILC1s was significantly increased,the level of HMGB1 was significantly upregulated,and that of miR-142-3p was considerably downregulated in the abortive mice as compared with the normal pregnant mice(all P<0.05).In addition,miR-142-3p was found to drastically inhibit the activation of the NF-κB signaling pathway(P<0.05).The number of ILC1s and the levels of pro-inflammatory cytokines were significantly downregulated and the activation of the NF-κB signaling pathway was inhibited in the miR-142-3p Agomir group(all P<0.05).Conclusion miR-142-3p can regulate ILC1s by targeting HMGB1 via the NF-κB signaling pathway,and attenuate the inflammation at the maternal-fetal interface in abortive mice.

Time-domain nature of group delay

The characteristic of group delay is analyzed based on an electronic circuit, and its time-domain nature is studied with time-domain simulation and experiment. The time-domain simulations and experimental results show that group delay is the delay of the energy center of the amplitude-modulated pulse, rather than the propagation delay of the electromagnetic field. As group velocity originates from the definition of group delay and group delay is different from the propagation delay, the superluminaiity or negativity of group velocity does not mean the supeduminal or negative propagation of the electromagnetic field.

Optical soliton with group delay in microring coupled-resonator optical waveguides

This paper derives the dispersion relation of microring coupled-resonator optical waveguides （CROWs） without any approximation by using the transfer matrix method. Based on the established dispersion relation of CROWs it obtains the slow group velocity and dispersion coefficient. It finds that the effect Of dispersion on optical pulses can be adjusted to balance the effect of nonlinearity by changing coupling coefficient or loss, so optical soliton with group delay can be obtained in microring CROWs. The optical soliton with group delay is of great significance for applications of microring CROWs in delay lines and optical buffers of future all-optical communication systems.

Optical time-domain differentiation based on intensive differential group delay

An optical time-domain differentiation scheme is proposed and demonstrated based on the intensive differential group delay in a high birefringence fibre waveguide. Results show that the differentiation waveforms agree well with the mathematically calculated derivatives. Both error and efficiency will increase when the birefringence fibre becomes longer, and the error rises up more quickly while the efficiency approaches to a maximum of ~0.25. By using a 1-m birefringence fibre a lower error of ~0.26% is obtained with an efficiency of 1% for the first-order differentiation of 10-ps Gaussian optical pulses, and the high-order optical differentiation up to 4th order is achieved with an error less than 3%. Due to its compact structure being easy to integrate and cascade into photonic circuits, our scheme has great potential for ultrafast signal processing.

A large bandwidth photonic delay line using passive cascaded silicon-on-insulator microring resonators

This paper investigated the design and the characterization of a photonic delay line based on passive cascaded silicon-on-insulator （SOI） microrings. We considered the compromise of group delay, bandwidth and insertion loss. A 3-stage double channel side-coupled integrated spaced sequence of resonator （SCISSOR） device was optimized by shifting the resonance of each microring and fabricated with electron beam lithography and dry etching. The group delay was measured to be 17 ps for non-return-to-zero signals at different bit rates and the bandwidth of 78 GHz was achieved. The experiment result agreed well with our simulation.

Stability and oscillations in a slow-fast flexible joint system with transformation delay

Flexible joints are usually used to transfer velocities in robot systems and may lead to delays in motion transformation due to joint flexibility. In this paper, a linkrotor structure connected by a flexible joint or shaft is firstly modeled to be a slow-fast delayed system when moment of inertia of the lightweight link is far less than that of the heavy rotor. To analyze the stability and oscillations of the slowfast system, the geometric singular perturbation method is extended, with both slow and fast manifolds expressed analytically. The stability of the slow manifold is investigated and critical boundaries are obtained to divide the stable and the unstable regions. To study effects of the transformation delay on the stability and oscillations of the link, two quantitatively different driving forces derived from the negative feedback of the link are considered. The results show that one of these two typical driving forces may drive the link to exhibit a stable state and the other kind of driving force may induce a relaxation oscillation for a very small delay. However, the link loses stability and undergoes regular periodic and bursting oscillation when the transformation delay is large. Basically, a very small delay does not affect the stability of the slow manifold but a large delay affects substantially.

基于group delay函数的分布式阵列三角定位算法

为了提高信源三角定位的定位精度和噪声鲁棒性,采用群时延(group delay)函数和分布式阵列,提出一种高精度的三角定位算法。通过group delay函数的负差分形式与传统MUSIC空间谱的乘积消除无关伪峰,得到高精度DOA估计,然后根据三角定位方程和阵列模型得到精确信源位置估计,再利用分布式阵列的阵列孔径大、对内部子阵位置变化不敏感等特性,提高三角定位算法对噪声的鲁棒性。实验结果表明,该算法是一种精度高、鲁棒性强的信源三角定位算法。

A New Characterization of Simple K_3-groups

In this paper, We show that the simple K\-3-groups can be characterized by the orders of their maximal abelian subgroups. That is, we have Theorem Let G be a finite group and M a simple K \-3-group. Then G is isomorphic to M if and only if the set of the orders of the maximal abelian subgoups of G is the same as that of M .

Renormalization group methods for a Mathieu equation with delayed feedback

This paper presents the application of the renormalization group （RG） methods to the delayed differential equation. By analyzing the Mathieu equation with time delay feedback, we get the amplitude and phase equations, and then obtain the approximate solutions by solving the corresponding RG equations. It shows that the approximate solutions obtained from the RG method are superior to those from the conventionally perturbation methods.

Effects of information transmission delay and channel blocking on synchronization in scale-free Hodgkin-Huxley neuronal networks

In this paper,we investigate the evolution of spatiotemporal patterns and synchronization transitions in dependence on the information transmission delay and ion channel blocking in scale-free neuronal networks.As the underlying model of neuronal dynamics,we use the Hodgkin-Huxley equations incorporating channel blocking and intrinsic noise.It is shown that delays play a significant yet subtle role in shaping the dynamics of neuronal networks.In particular,regions of irregular and regular propagating excitatory fronts related to the synchronization transitions appear intermittently as the delay increases.Moreover,the fraction of working sodium and potassium ion channels can also have a significant impact on the spatiotemporal dynamics of neuronal networks.As the fraction of blocked sodium channels increases,the frequency of excitatory events decreases,which in turn manifests as an increase in the neuronal synchrony that,however,is dysfunctional due to the virtual absence of large-amplitude excitations.Expectedly,we also show that larger coupling strengths improve synchronization irrespective of the information transmission delay and channel blocking.The presented results are also robust against the variation of the network size,thus providing insights that could facilitate understanding of the joint impact of ion channel blocking and information transmission delay on the spatiotemporal dynamics of neuronal networks.

Suppressive Influence of Time- Space White Noise on the Explosion of Solutions of Stochastic Fokker- Planck Delay Differential Equations

It is generally known that the solutions of deterministic and stochastic differential equations （SDEs） usually grow linearly at such a rate that they may become unbounded after a small lapse of time and may eventually blow up or explode in finite time. If the drift and diffusion functions are globally Lipschitz, linear growth may still be experienced, as well as a possible blow-up of solutions in finite time. In this paper, a nonlinear scalar delay differential equation with a constant time lag is perturbed by a multiplicative Ito-type time - space white noise to form a stochastic Fokker-Planck delay differential equation. It is established that no explosion is possible in the presence of any intrinsically slow time - space white noise of Ito - type as manifested in the resulting stochastic Fokker- Planck delay differential equation. Time - space white noise has a role to play since the solution of the classical nonlinear equation without it still exhibits explosion.

Luminescent properties of thermally activated delayed fluorescence molecule with intramolecular π-π interaction between donor and acceptor

Influence of intramolecular π-π interaction on the luminescent properties of thermally activated delayed fluorescence（TADF） molecule（3, 5-bis（3,6-di-tert-butyl-9 H-carbazol-9-yl）-phenyl）（pyridin-4-yl） methanone（DTCBPY） is theoretically studied by using the density functional theory（DFT） and time-dependent density functional theory（TD-DFT）.Four conformations（named as A, B, C, and D） of the DTCBPY can be found by relax scanning, and the configuration C corresponds to the luminescent molecule detected experimentally. Besides, we calculate the proportion of each conformation by Boltzmann distribution, high configuration ratios（44% and 52%） can be found for C and D. Moreover, C and D are found to exist with an intramolecular π-π interaction between one donor and the acceptor; the intramolecular interaction brings a smaller Huang-Rhys factor and reduced reorganization energy. Our work presents a rational explanation for the experimental results and demonstrates the importance of the intramolecular π-π interaction to the photophysical properties of TADF molecules.

Cloning and Sequence Analysis of Ma-14-3-3d Encoding a Homologue 14-3-3 Protein from Banana

[Objective] The aim of the study is to clone and analyze the gene encoding 14-3-3 protein from banana. [Method] Combined with PCR amplification, RACE (rapid amplification of cDNA ends) technique was employed to clone 14-3-3 gene from banana; then the amplified sequence was sequenced and homologically analyzed. [Result] A new cDNA homologous with 14-3-3 protein genes were obtained by RT-PCR and RACE ( rapid amplification of cDNA ends ) approaches. The full length of this cDNA was 866 bp encoding 197 amino acids. Alignment of deduced amino acid sequence with those from other plants revealed that the cDNA shared high homology with 14-3-3 protein genes from other plants, and was designated as Musa acuminata 14-3-3 gene (Ma-14-3-3d). Phylogenetic analysis reveals that Ma-14-3-3d has closer genetic relationship with those from monocotyledon species than those from other species. [Conclusion] Ma-14-3-3d belongs to the same lineage of 14-3-3 from monocotyledon.

胃癌组织高迁移率族蛋白A2与基质金属蛋白酶-9表达与肿瘤侵袭转移的关系及预后意义

目的探讨胃癌组织中高迁移率族蛋白A2(HMGA2)及基质金属蛋白酶-9(MMP-9)表达与肿瘤侵袭和转移能力的关系及预后意义。方法采用免疫组化法(SP法)检测93例胃癌组织、30例正常胃黏膜组织中HMGA2、MMP-9的表达;收集患者临床病历资料,并进行随访。结果 HGMA2蛋白在胃癌组织中阳性表达率分别为明显高于正常胃黏膜(P<0.01);MMP-9蛋白在胃癌组织中阳性表达率明显高于正常胃黏膜(P<0.01)。HMGA2与MMP-9在胃癌组织中的蛋白阳性表达均与胃癌组织的肿瘤浸润深度、肿瘤分化程度、淋巴结转移、TNM分期有关(P<0.05);与患者的性别、年龄、肿瘤直径无关(P>0.05)。相关性分析发现,HMGA2与MMP-9在胃癌组织中的表达情况呈正相关(r=0.317,P<0.01)。经Kaplan-Meier生存分析显示,HMGA2、MMP-9蛋白表达阳性患者的生存率均低于表达阴性患者(P<0.01)。结论 HMGA2、MMP-9与胃癌浸润和转移有关,两者表达具有相互协同作用,对胃癌的侵袭和转移起重要的促进作用。HMGA2、MMP-9是影响预后的危险因素,可能成为胃癌治疗的新靶点。

丙泊酚对急性肺损伤大鼠HMGB-1表达的影响

目的探讨丙泊酚的肺保护作用及其可能作用机制。方法采用尾静脉注射内毒素建立急性肺损伤模型,丙泊酚和锌原卟啉同样经过尾静脉给药。将Wistar大鼠按随机数字表法分为实验对照组(C组)、脂多糖组(L组)、脂多糖+丙泊酚组(LP组)。C组尾静脉注射生理盐水;L组尾静脉注射LPS 7.5mg·kg-1;LP组尾静脉注射LPS 7.5mg·kg-1,同时静脉注射丙泊酚30mg·kg-1。给药前及开始后第3、6和12h测定动脉血氧分压(PaO2),实验结束后观察肺湿/干重(W/D)比值、肺组织病理学检查并进行肺损伤轻重程度评分,同时观察各组大鼠肺组织高迁移率族蛋白1(HMGB-1)含量。结果实验前各组PaO2无明显差异,注射LPS后L组PaO2持续下降,和C组相比其PaO2显著降低(P<0.05)。实验结束后L组和C组相比其W/D比值、肺组织病理学检查评分、HO-1及HMGB-1含量显著增加(P<0.05)。而LP组和L组相比其PaO2显著改善,而W/D比值、肺组织病理学检查评分及HMGB-1含量显著降低(P<0.05)。结论丙泊酚具有肺保护作用,且该作用可能与其抑制HMGB-1过度表达作用相关。

SSH环境下的销售“3-1分栏报表”生成技术与算法研究

针对某集团ERP系统SSH(Strust,Spring,Hibernate)环境下需要研发销售"3-1分栏报表"时遇到的数据操作过于频繁和算法复杂度太高的问题,给出了解决问题的思路,以业务员、区域、客户、产品系列4个分栏使用"Groupby"HQL语句生成List集合,再转换为二维字符串数组,进而给出了算法假设、设计和实现,最终生成数据矩阵,使复杂度降低为O(n3),经测试该方法比"通常方法"约快19倍。

Blood group type antigens in pancreatic intraductal papillary mucinous neoplasms

BACKGROUND: There are few data on blood group(BG) types and types of pancreatic cancers. The aims of this study were to study BG types and BG-antigens in pancreatic intraductal papillary mucinous neoplasms(IPMNs). METHODS: BG type and tumor BG-antigen(glycoprotein) expression(studied by immunohistochemistry on tissue microarrays) were analyzed with regard to characteristics of 101 surgically resected pancreatic IPMNs. RESULTS: Non-O BG type predicted invasive carcinoma independently from high serum CA19-9 and male gender. BG type A was observed more frequently in women than in men. Chronic pancreatitis was more frequently seen in patients with BG type B or AB. Aberrant tumor expression(with regard to BG type) of loss of A antigen expression type occurred in 15.0% of IPMNs and of loss of B antigen expression type in 62.5% of IPMNs. Intraneoplasm BG-antigen expression was not related to dysplasia grade or invasion. CONCLUSION: The results of the study suggest that in pancreatic IPMN, non-O BG type predicted invasive carcinoma, whereas for intratumor BG-antigen expression no specific patterns were detected with regard to the progression of glandular epithelial dysplasia or invasion.