Bone marrow-derived mesenchymal stem cell-derived exosomeloaded miR-129-5p targets high-mobility group box 1 attenuates neurological-impairment after diabetic cerebral hemorrhage

BACKGROUND Diabetic intracerebral hemorrhage(ICH)is a serious complication of diabetes.The role and mechanism of bone marrow mesenchymal stem cell(BMSC)-derived exosomes(BMSC-exo)in neuroinflammation post-ICH in patients with diabetes are unknown.In this study,we investigated the regulation of BMSC-exo on hyperglycemia-induced neuroinflammation.AIM To study the mechanism of BMSC-exo on nerve function damage after diabetes complicated with cerebral hemorrhage.METHODS BMSC-exo were isolated from mouse BMSC media.This was followed by transfection with microRNA-129-5p(miR-129-5p).BMSC-exo or miR-129-5poverexpressing BMSC-exo were intravitreally injected into a diabetes mouse model with ICH for in vivo analyses and were cocultured with high glucoseaffected BV2 cells for in vitro analyses.The dual luciferase test and RNA immunoprecipitation test verified the targeted binding relationship between miR-129-5p and high-mobility group box 1(HMGB1).Quantitative polymerase chain reaction,western blotting,and enzyme-linked immunosorbent assay were conducted to assess the levels of some inflammation factors,such as HMGB1,interleukin 6,interleukin 1β,toll-like receptor 4,and tumor necrosis factorα.Brain water content,neural function deficit score,and Evans blue were used to measure the neural function of mice.RESULTS Our findings indicated that BMSC-exo can promote neuroinflammation and functional recovery.MicroRNA chip analysis of BMSC-exo identified miR-129-5p as the specific microRNA with a protective role in neuroinflammation.Overexpression of miR-129-5p in BMSC-exo reduced the inflammatory response and neurological impairment in comorbid diabetes and ICH cases.Furthermore,we found that miR-129-5p had a targeted binding relationship with HMGB1 mRNA.CONCLUSION We demonstrated that BMSC-exo can reduce the inflammatory response after ICH with diabetes,thereby improving the neurological function of the brain.

智能群体多维感知机器人支持的人机协同研学教学模式--以“孔子”大语言模型会话机器人的研发与教学为例

在大语言模型教育应用中,人机会话反馈形式单一问题一直未得到较好解决,这严重影响了学生的高阶思维能力培养。开发具有智能群体多维感知能力的会话机器人,是解决这一问题的重要途径。为此,研究者以群体感知、同伴反馈和研究性学习理论为基础,利用文心一言、飞书开发平台和学习分析等技术,开发了智能群体多维感知会话机器人“孔子”,构建了人机协同研学教学模式。教学实验以“现代教育技术应用”课程为教学内容,采用准实验研究设计,邀请数学专业学生参与实验。结果表明,与直接使用文心一言的教学相比,人机协同研学教学模式能显著提升学生的问题解决能力、批判性思维能力、创新能力和外在学习动机,但对学习绩效、内在动机和协作学习能力效果欠佳。为了提高教学效果,研究者需提高多维感知评价精准性、感知维度全面性、情境学习多样性和教师教学技能。

MiR-142-3p Regulates ILC1s by Targeting HMGB1 via the NF-κB Pathway in a Mouse Model of Early Pregnancy Loss

Objective Innate lymphoid cells(ILCs)are a class of newly discovered immunocytes.Group 1 ILCs(ILC1s)are identified in the decidua of humans and mice.High mobility group box 1(HMGB1)is predicted to be one of the target genes of miR-142-3p,which is closely related to pregnancy-related diseases.Furthermore,miR-142-3p and HMGB1 are involved in regulating the NF-κB signaling pathway.This study aimed to examine the regulatory effect of miR-142-3p on ILC1s and the underlying mechanism involving HMGB1 and the NF-κB signaling pathway.Methods Mouse models of normal pregnancy and abortion were constructed,and the alterations of ILC1s,miR-142-3p,ILC1 transcription factor(T-bet),and pro-inflammatory cytokines of ILC1s(TNF-α,IFN-γand IL-2)were detected in mice from different groups.The targeting regulation of HMGB1 by miR-142-3p in ILC1s,and the expression of HMGB1 in normal pregnant mice and abortive mice were investigated.In addition,the regulatory effects of miR-142-3p and HMGB1 on ILC1s were detected in vitro by CCK-8,Annexin-V/PI,ELISA,and RT-PCR,respectively.Furthermore,changes of the NF-κB signaling pathway in ILC1s were examined in the different groups.For the in vivo studies,miR-142-3p-Agomir was injected in the uterus of abortive mice to evaluate the abortion rate and alterations of ILC1s at the maternal-fetal interface,and further detect the expression of HMGB1,pro-inflammatory cytokines,and the NF-κB signaling pathway.Results The number of ILC1s was significantly increased,the level of HMGB1 was significantly upregulated,and that of miR-142-3p was considerably downregulated in the abortive mice as compared with the normal pregnant mice(all P<0.05).In addition,miR-142-3p was found to drastically inhibit the activation of the NF-κB signaling pathway(P<0.05).The number of ILC1s and the levels of pro-inflammatory cytokines were significantly downregulated and the activation of the NF-κB signaling pathway was inhibited in the miR-142-3p Agomir group(all P<0.05).Conclusion miR-142-3p can regulate ILC1s by targeting HMGB1 via the NF-κB signaling pathway,and attenuate the inflammation at the maternal-fetal interface in abortive mice.

5-19 Research Progress in Group of RIB Physics in 2015

The progress of research going on in Group of Rare Isotope Beam Physics (RIB), Experimental Physics Research Center, in 2015 is in the following two aspects. Construction of the plastic scintillator detector array (PSD) for the DArk Matter Particle Explorer (DAMPE) project.DAMPE is part of the “Strategic Priority Research Program” of the Chinese Academy of Science for space science, and it is a large space telescope aimed for the evidence of dark matter existence through the measurements of their decay products.

3-26 Research Progress in Group of Energy Materials in 2015

The fracture behavior is a very important aspect of the mechanical properties of structure materials. For highenergy ions, their range in steels can be significantly larger than the average size of grains of steels, thus their irradiation can change the bulk properties of steels. It is therefore possible to study the fracture behavior of steel specimens irradiated with high-energy ions.

2-27 Research Progress in Group of RIB Physics in 2014

The progress of research going on in Group of Rare Isotope Beam Physics (RIB), Experimental Physics ResearchCenter, in 2014 is in the following three aspects.1. Detector construction for the Dark Matter Particle Explorer (DAMPE) project. DAMPE is a large spacetelescope aimed at the evidence of dark matter existence through the measurements of their decay products. It isa part of the "Strategic Priority Research Program" of the Chinese Academy of Sciences for space science, and willbe launched in 2015. In this project, the RIB group is involved in the design and construction of the plastic scintillatorFig. 1 (color online) Accumulative adsorp-tion capacity of uranium from brine.detector array (PSD), which is a key component of theload with an active area of 0.8 m  0.8 m.

2-1 Research Progress of Nuclear Structure Research Group

The Group of Nuclear Structure Research at IMP has devoted much effort to the lifetime measurements of lowlying excited states via ?? fast timing coincidence. Two experiments were performed in 2015 at the radioactive beam line RIBLL[1] and gas-filled recoil separator SHANS[2].

AARC-ACLF评分联合NLR对HBV相关慢加急性肝衰竭短期预后的评估价值

目的探究亚太肝脏研究协会ACLF(AARC-ACLF)评分联合中性粒细胞与淋巴细胞比值(NLR)对HBV相关慢加急性肝衰竭(ACLF)短期预后的预测价值。方法选择94例HBV相关ACLF患者作为研究对象,于入组24 h内检测NLR,计算AARC-ACLF评分。根据临床疗效及生存情况,分为转归良好组及不佳组。分析HBV相关ACLF患者病情转归的影响因素。评估AARC-ACLF评分和血清NLR水平对HBV相关ACLF预后的预测效能。结果94例受试者中,转归良好58例,转归不佳36例。转归不佳组凝血酶原时间(PT)、总胆红素(TBil)、白细胞(WBC)、NLR及AARC-ACLF评分分别为(26.68±6.91)s、(330.2±126.4)μmol/L、(6.67±2.06)×10^(9)/L、6.45±2.76、(10.14±1.29)分,转归良好组为(20.32±4.20)s、(180.1±83.7)μmol/L、(5.06±1.97)×10^(9)/L、2.85±1.67、(8.03±1.09)分,差异有统计学意义(P<0.05)。NLR及AARC-ACLF评分偏高均为HBV相关ACLF患者病情转归不佳的影响因素(P<0.05);血清NLR、AARC-ACLF评分、NLR联合AARC-ACLF评分预测HBV相关ACLF患者预后的AUC分别为0.878、0.892、0.943。结论HBV相关ACLF患者的血清NLR及AARC-ACLF评分均较高,对该病的短期预后具有一定的临床预测价值,且两者联合预测效能更高。

我国大学生就业研究知识图谱(2003-2021年):基于CiteSpace的计量分析

文章以CSSCI中2003—2021年2330篇大学生就业相关的研究论文为研究对象,以信息可视化软件CiteSpace 6.1为工具,通过文献共被引、关键词共现及突现词分析,对我国大学生就业研究的状况进行了分析。研究发现:我国大学生就业研究选题紧密围绕国家政策和现实背景,具有较强的现实性;研究主题发散,但呈现多元化交融的特征;研究发展过程具有明显的阶段性特征,在经历了初始发展阶段、深化发展阶段后,目前正处于蓄势阶段;研究内容关注社会维度、经济维度、教育维度等对大学生就业的全方位影响,并试图理顺政府、高校、家庭和学生个体在“学业—就业”转变过程中的角色和地位。

The most important questions in cancer research and clinical oncology Question 2-5.Obesity-related cancers:more questions than answers

Obesity is recognized as the second highest risk factor for cancer. The pathogenic mechanisms underlying tobaccorelated cancers are well characterized and efective programs have led to a decline in smoking and related cancers, but there is a global epidemic of obesity without a clear understanding of how obesity causes cancer. Obesity is heterogeneous, and approximately 25% of obese individuals remain healthy(metabolically healthy obese, MHO), so which fat deposition(subcutaneous versus visceral, adipose versus ectopic) is "malignant"? What is the mechanism of carcinogenesis? Is it by metabolic dysregulation or chronic inflammation? Through which chemokines/genes/signaling pathways does adipose tissue influence carcinogenesis? Can selective inhibition of these pathways uncouple obesity from cancers? Do all obesity related cancers(ORCs) share a molecular signature? Are there common(overlapping) genetic loci that make individuals susceptible to obesity, metabolic syndrome, and cancers? Can we identify precursor lesions of ORCs and will early intervention of high risk individuals alter the natural history? It appears unlikely that the obesity epidemic will be controlled anytime soon; answers to these questions will help to reduce the adverse efect of obesity on human condition.

A New Characterization of Simple K_3-groups

In this paper, We show that the simple K\-3-groups can be characterized by the orders of their maximal abelian subgroups. That is, we have Theorem Let G be a finite group and M a simple K \-3-group. Then G is isomorphic to M if and only if the set of the orders of the maximal abelian subgoups of G is the same as that of M .

The Nanjing International Symposium of New Frontiers in Cancer Research(Nov 9-10,2007)

The Nanjing International Symposium of New Frontiers in Cancer Research will be held on Nov 9-10, 2007. The purpose of this symposium is to stimulate a dialogue among basic and clinical cancer researchers between China and other countries to speed up translation of new findings in basic cancer biology into disease prevention, diagnosis, and treatment. We hope to use this conference as a forum to create a unique opportunity for exchange in current discoveries, fostering multidisciplinary collaborations, and identification of future opportunities in cancer treatment and patient care. There will be four sessions in this symposium：

CONFERENCE At-A-Glance SOCIETY FOR ACUPUNCTURE RESEARCH“Translational Research in Acupuncture:Bridging Science,Practice and Community” Chapel Hill,NC,March 19-21,2010

THURSDAY,March 18 Pre-Conference Workshops"lntroduction to current issues in acupunclure research""ConsideratiOns for developing COllaboration research between AOM COlleges and conventionaI medicalinstitutions"

Research on Ideological and Political Education of Higher Vocational College Students' Network Groups in the New Era

With the rapid development of modern information technology, Internet technology has become a major driver of social and economic development. It also has a great influence on higher vocational school education, especially the ideological and political education of young students. The network has brought new changes to the students' cognition, thoughts and conduct, learning methods, and physical and mental development. At the same time, it has a negative effect on students' own development and student group development. In order to better serve the ideological and political education of higher vocational students and improve the pragmaticality and effectiveness of the ideological and political work, we have actively enriched the network carriers, took the initiative to fight for Internet public opinion, actively built a network response mechanism, and focus on the training of network leaders and other measures to strengthen the online community ideological and political education.

Physical-Chemical and Pharmacological Research on New Lignin Enterosorbent

The technology on obtaining enterosorbent from local feedstock--hydrolyzed lignin of cotton seed hulls named Zerotox was developed. The purpose of this research is to conduct a complex study on its physical-chemical and pharmacological properties. Samples of cotton hydrolyzed lignin based enterosorbent Zerotox were investigated, wood hydrolyzed lignin based enterosorbent Polifepan （Russia） was used as a comparison drug. The technology of new medical enterosorbent Zerotox, based on cotton hydrolyzed lignin, has been developed with its physical-chemical and pharmacological characteristics being researched. Comparative research on adsorbing capacity of sorbent Zerotox （80 mg/g） and its analogue--Polipefan （55 mg/g）, which is obtained from wood hydrolyzed lignin, has displayed advantages of the new sorbent. IR-spectroscopy research on lignin sorbent Zerotox has shown presence of oxygen-containing functional groups which determine its high absorbing capacity due to its chemisorption. Atomic-adsorptive method has proved high absorbing capacity of the sorbent towards heavy metals ions. Acute and chronic toxicity and antidiarrheal activity of enterosorbent Zerotox have also been studied.

Quantum Research Group2和4按键触摸传感器

Quantum Research Group公司推出的QTouch QT220和QT240电荷转移数字传感器芯片在批量消费类应用中每按键的成本大约为15美分，为采用人机界面的设备制造商提供与采用机电开关价格相近的方案。这些按键具备在2个或4个电极上进行近距感应或触摸的能力，这些电极无论通过任何介质都可以反映出独立的感测区域的信号，包括玻璃、塑料、石材、陶瓷或者木材。

SGZ730-800系列刮板输送机链轮轴组修复工艺研究

SGZ730-800系列刮板输送机在煤矿生产中有着非常广泛的应用。链轮轴组是刮板输送机驱动链条输送煤炭的核心部件,也是经常更换、维修的部件。链轮轴组处于周期运转中,出现链窝、轮齿磨损严重以及链轮锈蚀现象,造成后期的拆解、修复工作十分困难。主要研究链轮轴组磨损后的修复和不易拆解时的维修工艺,以提高链轮轴组使用寿命和维修效率。

省级基础研究创新群体项目资助回顾与思考--以甘肃省为例

甘肃省为打造并培养出在国际、国内相关研究领域占有一席之地的高层次研究团队,汇聚若干名甘肃省优秀中青年科技人才、一流的学科带头人,围绕同一个领域在几个研究方向开展长期稳定的合作研究,设立甘肃省基础研究创新群体项目。通过回顾国家自然科学基金委创新研究群体项目和甘肃省基础研究创新群体项目设立的背景及意义,多维度分析2012-2021年资助的甘肃省基础研究创新群体项目相关数据,深刻分析问题产生的原因,并提出合理的意见和建议,旨在为省级主管部门、依托单位和创新群体提供重要的参考依据。