Arugula(Eruca sativa Mill.),as an edible medicinal vegetable of peculiar flavor,is served as uncooked dish.The influence of variable lighting intensity(LI)on the growth characteristics and nutritional quality of hydro...Arugula(Eruca sativa Mill.),as an edible medicinal vegetable of peculiar flavor,is served as uncooked dish.The influence of variable lighting intensity(LI)on the growth characteristics and nutritional quality of hydroponically grown arugula was investigated by using light-emitting diodes(LEDs)to light the hydroponically grown arugula for a reference for industrialized arugula production.The dynamic demands of arugula for LI in the seedling stage,initial growth stage and vigorous growth stage were tested under two light quality conditions including a red/blue light ratio of 7:1 and a light/dark photoperiod of 12 h/12 h.Then,the curves of variable LI-induced changes in the growth indices of arugula in different development periods were drawn.Next,the influence of variable LI on the growth characteristics and nutritional quality of arugula was investigated by measuring the dry/fresh weight ratio,chlorophyll content,vitamin C content and soluble protein content.Variable LI significantly increased the height,stem diameter,leaf width,dry/fresh weight ratio,chlorophyll content and soluble protein content of arugula plant.Plant height,stem diameter,dry/fresh weight ratio,chlorophyll content and soluble protein content were the highest in the group exposed to LI of 200,300 and 300μmol•m^(-2)•s^(-1)during the seedling stage,initial growth stage and vigorous growth stage,respectively.The greatest leaf width was achieved at LI of 100,250 and 350μmol•m^(-2)•s^(-1),respectively.High intensity LI markedly repressed the synthesis of vitamin C.展开更多
A long-term field experiment (started at 2003) was conducted to determine the effects of different dce cultivation methods on growth characteristics and grain yield of late-season rice under double-rice cropping sys...A long-term field experiment (started at 2003) was conducted to determine the effects of different dce cultivation methods on growth characteristics and grain yield of late-season rice under double-rice cropping system in seasonal drought region of southeast China (Yujiang County, Jiangxi Province). The rice cultivation methods included no-tillage and flooded rice cultivation (N-F), no-tillage and non-flooded rice cultivation with straw mulching (N-SM), and no-tillage and non-flooded rice cultivation without straw mulching (N-ZM). There was no significant difference in rice grain yield between the N-SM and N-F treatments. However, the rice grain yields in the N-SM and N-F treatments were significantly higher than that in the N-ZM treatment. The late-season rice plants in the N-SM treatment had significantly higher numbers of effective panicles and total grains per hill compared with those in the N-ZM treatment. The above-ground dry matter of late-season rice was similar between the N-SM and N-F treatments. Compared with the N-F treatment, the N-ZM and N-SM treatments significantly decreased the leaf area at the heading stage. Moreover, the N-SM treatment could significantly increase total root length and root tip number at the grain-filling stage compared with the N-ZM treatment.展开更多
[Objectives] This study was conducted to discuss the possibility of applying microbial fertilizer to production of facility tomato,so as to determine rational application rate for facility tomato. [Methods]A field exp...[Objectives] This study was conducted to discuss the possibility of applying microbial fertilizer to production of facility tomato,so as to determine rational application rate for facility tomato. [Methods]A field experiment was carried out to investigate the effects of the application of microbial fertilizer onto acidic soil on yield,quality and soil chemical properties of facility tomato. [Results] Yield of facility tomato was improved after the application of microbial fertilizer.Treatment ABA-2 showed the highest yield,which was higher than the CK by 7. 98%. On the basis of conventional fertilization,the combined application with microbial fertilizer could promote growth and development of tomato,and could significantly improve fruit weight,and Vc,soluble solid and lycopene contents. Furthermore,the microbial fertilizer could remarkably improve soil p H,alkali-hydrolyzale nitrogen,available phosphorus and rapidly available potassium contents.[Conclusions]The application of microbial fertilizer at 80 kg/667 m;has better effects on tomato growth and development and soil status.展开更多
We estimated the growth volume of artificially reforested Pinus densiflora in a post-fire area on three different contour conditions by comparing and analyzing the vertical and horizontal distributions of below-ground...We estimated the growth volume of artificially reforested Pinus densiflora in a post-fire area on three different contour conditions by comparing and analyzing the vertical and horizontal distributions of below-ground roots on each contour. The main roots at the south-facing slope (SS) developed in a long and straight form, and those on the north-facing slope (NS) in a twisted form. The side roots developed more than the main roots on the Ridge. The depth of taproots decreased in the following order: SS?>?NS?>?Ridge. The roots on the SS developed in a pile-form root structure whereas those in the Ridge and NS developed concentrically near the root collar. The amount of root development decreased in the following order: SS?>?NS?>?Ridge. The ratio of fine roots from the whole-root development decreased in the following order: Ridge?>?SS?>?NS. These results can guide considerations of growth differences according to the planting contour conditions for future establishment of P. densiflora artificial plantations.展开更多
The growth characteristics of Aspergillus parasitic us incubated on two culture media were ex-amined using shortwave infrared(SWIR,1000-2500 nm)hyperspectral imaging(HSI)in this work.HSI images of the A.parasiticus co...The growth characteristics of Aspergillus parasitic us incubated on two culture media were ex-amined using shortwave infrared(SWIR,1000-2500 nm)hyperspectral imaging(HSI)in this work.HSI images of the A.parasiticus colonies growing on rose bengal medium(RBM)and maize agar medium(MAM)were recorded daily for 6 days.The growth phases of A.parasiticus were indicated through the pixel number and average spectra of colonies.On score plot of the first principal component(PC1)and PC2,four growth zones with varying mycelium densities were identified.Eight characteristic wavelengths(1095,1145,1195,1279,1442,1655,1834 and 1929 nm)were selected from PC1 loading,average spectra of each colony as well as each growth zone.F urthermore,support vector machine(S VM)classifier based on the eight wavelengths was built,and the classification accuracies for the four zones(from outer to inner zones)on the colonies on RBM were 99.77%,9935%,99.75%and 99.60%and 99.77%,9939%,99.31%and 98.22%for colonies on MAM.In addition,a new score plot of PC2 and PC3 was used to differ-entiate the colonies incubated on RBM and MAM for 6 days.Then characteristic wavelengths of 1067,1195,1279,1369,1459,1694,1834 and 1929 nm were selected from the loading of PC2 and PCg.Based on them,a new SVM model was developed to diferentiate colonies on RBM and MAM with accuracy of 100.00%and 9999%,respectively.In conclusion,SWIR hyperspectral image is a powerful tool for evaluation of growth characteristics of A.parasiticus incubated in diferent culture media.展开更多
The tea plant is a valuable and evergreen crop that is extensively cultivated in China and many other countries.Currently,there is growing research interest in this plant.For the tea industry,it is crucial to develop ...The tea plant is a valuable and evergreen crop that is extensively cultivated in China and many other countries.Currently,there is growing research interest in this plant.For the tea industry,it is crucial to develop rapid and non-invasive methods to evaluate tea plants in their natural environment.This article provides a comprehensive overview of non-invasive sensing techniques used for in-situ detection of tea plants.The topics covered include leaf,canopy,and field-level assessments,as well as statistical analysis techniques and characteristics specific to the research.Non-invasive testing technology is primarily used for monitoring and predicting tea pests and diseases,monitoring quality,and nutrients,determining tenderness and grade,identifying tea plant varieties,automatically detecting,and identifying tea buds,monitoring tea plant growth,and extracting tea garden areas through remote sensing.It also helps to evaluate planting suitability,assess disasters,and estimate yields.Additionally,the article examines the challenges and prospects of emerging techniques aimed at resolving the in-situ detection problem for tea plants.It can assist researchers and producers in comprehensively understanding the tea environment,quality characteristics,and growth process,thereby enhancing tea production quality,and fostering tea industry development.展开更多
The use of titanium dioxide nanoparticles (nTiO<sub>2</sub>) is gaining interest in agriculture because of their impact on many aspects of plant growth. The present study examines the effects of nTiO<su...The use of titanium dioxide nanoparticles (nTiO<sub>2</sub>) is gaining interest in agriculture because of their impact on many aspects of plant growth. The present study examines the effects of nTiO<sub>2</sub> (5 nm and 10 nm) applied to seeds and the seedlings as a foliar application on various aspects of growth characteristics and biomass accumulation in lettuce (Lactuca sativa, cv. Grand Rapids). Application of 10 nm nTiO<sub>2</sub> to seeds through imbibition resulted in a significant reduction in shoot biomass accumulation while 5 nm nTiO<sub>2</sub> did not affect the biomass accumulation in lettuce. The application of 10 nm nTiO<sub>2</sub> reduced the fresh shoot biomass accumulation by about 18% compared to the control plants. Other growth characteristics such as shoot dry biomass, root fresh and dry biomass, plant height, and leaf area were not affected by the application of both 5 nm and 10 nm nTiO<sub>2</sub>. In addition, foliar application of these nanoparticles to the lettuce seedlings did not have a significant effect on most of the growth parameters examined, and the increasing concentration ranging from 5 nm/L to 400 mg/L did not produce a consistent response in lettuce. Thus, nTiO<sub>2</sub> application to lettuce seeds had a notable negative impact on shoot growth while foliar application did not have a significant effect on many plant growth characteristics. However, foliar applications produced some symptoms of toxicity to the foliage in the form of necrotic or chlorotic patches on the leaves, which were more pronounced with increasing concentrations of both 5 nm and 10 nm nTiO<sub>2</sub>. However, these symptoms were apparent at a concentration as low as 50 mg/L of nTiO<sub>2</sub>. Thus, foliar application of nTiO<sub>2</sub> may not have a significant impact on many of the growth characteristics in lettuce, but it can result in foliar toxicity.展开更多
Under harsh conditions (such as high temperature, high pressure, and millisecond lifetime chemical reaction), a long-standing challenge remains to accurately predict the growth characteristics of nanosize spherical ...Under harsh conditions (such as high temperature, high pressure, and millisecond lifetime chemical reaction), a long-standing challenge remains to accurately predict the growth characteristics of nanosize spherical particles and to determine the rapid chemical reaction flow field characteristics, The growth characteristics of similar spherical oxide nanoparticles are further studied by successfully introducing the space-time conservation element-solution element (CE/SE) algorithm with the monodisperse Kruis model. This approach overcomes the nanosize particle rapid growth limit set and successfully captures the characteristics of the rapid gaseous chemical reaction process. The results show that this approach quantitatively captures the characteristics of the rapid chemical reaction, nanosize particle growth and size distribution. To reveal the growth mechanism for numerous types of oxide nanoparticles, it is very important to choose a rational numerical method and particle physics model.展开更多
Xinjiang is the province with the largest saline-alkali land in China.The growth of halophytes will be stressed when their salt content reaches the threshold.In this study,the pot experiments were conducted and Suaeda...Xinjiang is the province with the largest saline-alkali land in China.The growth of halophytes will be stressed when their salt content reaches the threshold.In this study,the pot experiments were conducted and Suaeda salsa,a typical halophyte,was selected as the object to explore the relationship between growth characteristics and salt content under different soil salinity gradients.Salinity gradients were set according to the maximum soil salinity in the current years of Ebinur Lake Wetland National Nature Reserve(ELWNNR).They were classified using the following threshold values:≤2 g/kg(Control Group),10 g/kg,20 g/kg,30 g/kg,and 40 g/kg.The original spectrum,trilateral position and growth characteristics were used as indexes for analysis.With the increase of soil salt content,the red edge shift occurs first,followed by the blue edge shift.The position of yellow edge,green peak and blue edge were not sensitive to the change of soil salinity.This indicates that the red edge is the best indicator for evaluating plant growth.Compared with the Control Group,when the salt content is less than 10 g/kg,the growth status of plant seems not significantly affected.However,plant growth begins to be stressed when the salt content increases to 20 g/kg,which is a turning point for plant health.The increase of soil salt content can inhibit chlorophyll synthesis and plant growth.Plants begin to die when the salt content reaches 40 g/kg.Therefore,40 g/kg could be regarded as the critical point of salt content which inhibits plant growth.The study also shows that the relationship between plant-height change rate and soil salt content is the most significant,indicating that the plant-height change rate is significantly impacted by soil salt content.展开更多
Michelia macclurei Dandy is a significant tree species that has extensive cultivation for forestry and horticulture purposes in southern China,owing to its economic and practical importance.Light quality influences pl...Michelia macclurei Dandy is a significant tree species that has extensive cultivation for forestry and horticulture purposes in southern China,owing to its economic and practical importance.Light quality influences plantlet growth and development during tissue culture.However,the growth characteristic and molecular regulation of M.macclurei under different light quality conditions are not well understood yet.In this study,we investigated the morphological,chlorophyll content,and transcriptomic responses of M.macclurei plantlets under different light-emitting diode(LED)qualities,including white,blue,and red light.The results showed that blue light significantly increased plant height(21.29%)and leaf number(18.65%),while red light decreased plant height and leaf number by 7.53%and 16.49%,respectively.In addition,the plantlets’chlorophyll content and etiolation rate were significantly reduced by blue and red light quality compared to white light.Compared to white light,blue light had a negative effect,leading to decreased rooting rate(64.28%),root number(72.72%),and root length(75.86%).Conversely,red light had a positive effect,resulting in increased rooting rate(24.99%),root number(109.58%),and root length(72.72%).Transcriptome analysis identified 54 differentially expressed genes(DEGs)in three groups that consisted of blue light vs.white light(BL-vs-WL),red light vs.white light(RL-vs-WL),and red light vs.blue light(RL-vs-BL).Specifically,21,7,and 41 DEGs were identified in the three groups,respectively.The DEGs found in the RL-vs-WL and BL-vs-WL groups were involved in plant hormone signaling,nitrogen metabolism,and phenylpropanoid biosynthesis pathways,which suggests that M.macclurei plantlets adapt to the changes of light quality via modulating gene expression.Overall,our study provides valuable insights for understanding the molecular and morphological responses of M.macclurei plantlets under different light qualities.展开更多
[Objectives]The paper was to provide a reference for screening dwarfing rootstock suitable for main spur-type Fuji cultivars in central and southern Hebei Province.[Methods]With spur-type Fuji‘Tianhong 2’as the mate...[Objectives]The paper was to provide a reference for screening dwarfing rootstock suitable for main spur-type Fuji cultivars in central and southern Hebei Province.[Methods]With spur-type Fuji‘Tianhong 2’as the material,the vegetative growth,yield and fruit quality of 8 different rootstock-scion combinations were compared.[Results]‘Tianhong 2’/SH6 as self-rooted rootstock had large average single fruit weight(256.33 g),large number of fruits per plant(188.68),the highest yield[(3250.08±23.42)kg/667 m ^(2)]and the highest colored area(93.5%),and the soluble solid content reached the requirement of high quality fruit(15.78%).[Conclusions]In central and southern Hebei Province,‘Tianhong 2’grafted on SH6 self-rooted rootstock has moderate growth,high yield and good fruit quality,so it can be considered as the preferred rootstock-scion combination in the local area.展开更多
By using single rice cultivation as a control, the effect of rice-fish culture on growth dynamic, plant type and yield formation of rice was studied. The results showed as follows: rice-fish culture improved the phys...By using single rice cultivation as a control, the effect of rice-fish culture on growth dynamic, plant type and yield formation of rice was studied. The results showed as follows: rice-fish culture improved the physical-chemical properties of arable layer soil of paddy field, extended growth period of rice, increased dry matter and LAI of different growth stages, improved three top leaves area, deterred the degeneration of leaves function, increased the diameter of stem, promoted the growth of roots and the formation of roots in the extended stem. At the same time, rice-fish culture extended the length of basal internodes, increased the number of internodes, uplifted the gravity of plant, and depressed the root vigor. For the grain yield and yield structure office, rice-fish culture decreased ear/tillering ratio, spikelet/panicle and seed set percentage, increased grain weight. If variety choice and cultivation technology were controlled appropriately, rice-fish culture could increase the effective panicles and improve grain yield of rice.展开更多
Based on a series of polyploid indica-japonica hybrid plant lines obtained from a new breeding strategy of using double predominance of wide cross and polyploidization to breed super flee, two polyploid indica-japonic...Based on a series of polyploid indica-japonica hybrid plant lines obtained from a new breeding strategy of using double predominance of wide cross and polyploidization to breed super flee, two polyploid indica-japonica hybrids, PSR073 and PSR120 were studied in their growth periods to show the powerful heterosis in a larger scale and to study the characteristics of polyploid indica-japonica hybrids more elaborately. The leaf age, tiller growth, flowering habits, and agronomic traits of them were observed to analyze their growth habits and characteristics. The results showed that the.agronomic traits of PSR073 and PSR120, such as the plant height, panicle length, grain length, grain width, and 1000-grain weight, all acquired obvious predominance of polyploidy, and that the seed setting rate was more than 83%. No significant difference was observed between the two tetraploids and common diploids in the leaf age, tiller growth, and flowering habits. It was concluded that the characteristics of the two powerful heterosis polyploid hybrids were different from those of the polyploid rice reported earlier. Wide cross and polyploidization had no negative effects on their growth habits and characteristics; on the contrary these had powerful heterosis. This had provided theoretic and practical evidences for their application to agricultural production.展开更多
Wolfberry(Lycium barbarum L.)is important for health care and ecological protection.However,it faces problems of low productivity and resource utilization during planting.Exploring reasonable models for water and nitr...Wolfberry(Lycium barbarum L.)is important for health care and ecological protection.However,it faces problems of low productivity and resource utilization during planting.Exploring reasonable models for water and nitrogen management is important for solving these problems.Based on field trials in 2021 and 2022,this study analyzed the effects of controlling soil water and nitrogen application levels on wolfberry height,stem diameter,crown width,yield,and water(WUE)and nitrogen use efficiency(NUE).The upper and lower limits of soil water were controlled by the percentage of soil water content to field water capacity(θ_(f)),and four water levels,i.e.,adequate irrigation(W0,75%-85%θ_(f)),mild water deficit(W1,65%-75%θ_(f)),moderate water deficit(W2,55%-65%θ_(f)),and severe water deficit(W3,45%-55%θ_(f))were used,and three nitrogen application levels,i.e.,no nitrogen(N0,0 kg/hm^(2)),low nitrogen(N1,150 kg/hm^(2)),medium nitrogen(N2,300 kg/hm^(2)),and high nitrogen(N3,450 kg/hm^(2))were implied.The results showed that irrigation and nitrogen application significantly affected plant height,stem diameter,and crown width of wolfberry at different growth stages(P<0.01),and their maximum values were observed in W1N2,W0N2,and W1N3 treatments.Dry weight per plant and yield of wolfberry first increased and then decreased with increasing nitrogen application under the same water treatment.Dry weight per hundred grains and dry weight percentage increased with increasing nitrogen application under W0 treatment.However,under other water treatments,the values first increased and then decreased with increasing nitrogen application.Yield and its component of wolfberry first increased and then decreased as water deficit increased under the same nitrogen treatment.Irrigation water use efficiency(IWUE,8.46 kg/(hm^(2)·mm)),WUE(6.83 kg/(hm^(2)·mm)),partial factor productivity of nitrogen(PFPN,2.56 kg/kg),and NUE(14.29 kg/kg)reached their highest values in W2N2,W1N2,W1N2,and W1N1 treatments.Results of principal component analysis(PCA)showed that yield,WUE,and NUE were better in W1N2 treatment,making it a suitable water and nitrogen management mode for the irrigation area of the Yellow River in the Gansu Province,China and similar planting areas.展开更多
Here, the ozone-treated domestic sludge was diluted up to four different multiples and utilized as a nutritional source for hydroponic lettuce growth. Additionally, lettuce was cultured using the modified Hoagland nut...Here, the ozone-treated domestic sludge was diluted up to four different multiples and utilized as a nutritional source for hydroponic lettuce growth. Additionally, lettuce was cultured using the modified Hoagland nutrient solution as a control. The effects of ozone-treated domestic sludge on lettuce growth and nutrition were studied. Results showed that the lettuce treated with modified Hoagland inorganic nutrient solution had increased leaf number, plant height, fresh weight and dry weight compared to those treated with the ozone-treated domestic sludge dilution (P〈0.05). However, the lettuce cultivated with the 2-fold ozone-treated sludge dilution showed significantly higher (P〈0.05) contents of chlorophyll, soluble sugar and ascorbic acid (Vc) compared to that treated with modified Hoagland nutrient solution. And the nitrate concentration in the lettuce cultured with the 2-fold ozone-treated sludge dilution was 53.93% less than that cultured with the modified Hoagland nutrient solution, which was a significant improvement (P〈0.05). This study suggested that the 2-fold ozone-treated sludge dilution is optimal for lettuce hydroponic nutrient requirements.展开更多
Taking Cucurbita maxima and Cucurbita moschata as root stocks,and‘Jinyou No 3'cucumber as scion,the effects of different root zone temperature conditions optimal temperature( CK)( 18-20 ℃),suboptimal temperatur...Taking Cucurbita maxima and Cucurbita moschata as root stocks,and‘Jinyou No 3'cucumber as scion,the effects of different root zone temperature conditions optimal temperature( CK)( 18-20 ℃),suboptimal temperature( 13-15 ℃) and low temperature( 8-10 ℃) on the growth and photosynthesis indexes were studied. The results showed that,compared with optimal temperature( CK),suboptimal temperature and low temperature produced a significant inhibition of growth on cucumbers. The plant height,stem diameter,leaf area,number of leaves and dry weight of aboveground part were all reduced,dry weight of underground part and root shoot ratio all increased,while the inhibition was more significant at low temperature. Low and suboptimal temperature conditions also reduced SPAD value,net photosynthetic rate,transpiration rate,intercellular CO2 concentration and stomatal conductance of the grafted cucumber. And there were differences between different grafted seedlings,and seedlings with‘black seeds' as stock performed better than those with‘white seeds' as stock at low temperature.展开更多
[Objective] The paper was to study the effect of chemical-controlled topping technology on spectral and photosynthetic characteristics of cotton. [Method] Two row spacing configurations and four topping treatments wer...[Objective] The paper was to study the effect of chemical-controlled topping technology on spectral and photosynthetic characteristics of cotton. [Method] Two row spacing configurations and four topping treatments were set in the experiment field, and the spectral and photosynthetic characteristics of cotton under different configurations and topping treatments were analyzed. [Result] There was no significant difference in spectral reflectance of cotton leaves and canopy among all treatments(T1-T8) before topping. After topping, the spectral reflectance of cotton leaves showed little difference in visible light band, but significant differences in near-infrared light band under two row spacing configurations(T2-T4, T6-T8).And in near-infrared band, compared with CK(T1, T5), the reflectance increased and then decreased under close planting with equal row spacing configurations(T2-T4) and narrow row dense planting configurations(T6-T8). However, the change of cotton canopy reflectance in short infrared bands increased first and then decreased under close planting with equal row spacing configuration, and increased constantly under narrow row dense planting configuration. The photosynthetic capacity of leaves increased, and the net photosynthetic rate(A), stomatal conductance(GH_(2O)),transpirationrate(E) and intercellular CO_(2) concentration(Ci) significantly increased by controlling the top with chemicals, and differences were ob-served between two configurations. After topping treatments, the leaf area of apical leaf increased, and chemical-controlled topping treatments had significant difference with the control. The apical cotton leaves were shrunk internally, and the plant height increased gradually by 7-15 cm;the number of fruit branches increased by 2-4, and the area of apical leaves decreased significantly in chemical-controlled topping treatments.[Conclusion] After topping with chemicals, the spectral characteristics of cotton change, the photosynthetic capacity of leaves is improved, and the growth of apical leaves and apex of cotton is inhibited.展开更多
[Objectives] A field experiment was carried out with biogas manure replacing partial chemical fertilizer to discuss the effects on growth and development of summer maize and soil environment,so as to provide theoretic...[Objectives] A field experiment was carried out with biogas manure replacing partial chemical fertilizer to discuss the effects on growth and development of summer maize and soil environment,so as to provide theoretical basis for efficient utilization and zero growth of chemical fertilizer. [Methods]The summer maize field experiment was carried out in Muping District,Yantai City,with conventional fertilization as control,and sole application of biogas manure,biogas manure + 80% chemical fertilizer and biogas manure + 60% chemical fertilizer as three treatments. Growth indices,yield and yield components of summer maize and soil nutrient utilization status were determined. [Results] On the basis of reduced fertilizer application,the application of biogas manure could significantly promote growth of summer maize,and its plant height,stem diameter,ear position and dry weight per plant significantly increased compared with the CK. Remarkable yield increasing effect was obtained on summer maize,and among the various treatments,treatment BM + CF80 had the highest yield,which was 19. 91% higher than the CK. Furthermore,the combined application of biogas manure and chemical fertilizer not only could improve soil acidity( soil pH was improved by 0. 12 unit),but also could improve soil rapidly available potassium content and reduce soil alkali-hydrolyzable nitrogen content. [Conclusions]Comprehensively,combined application of biogas manure and 80% of chemical fertilizer is the best for growth and development of summer maize and improvement of soil character.展开更多
In modern terminology,“organoids”refer to cells that grow in a specific three-dimensional(3D)environment in vitro,sharing similar structures with their source organs or tissues.Observing themorphology or growth char...In modern terminology,“organoids”refer to cells that grow in a specific three-dimensional(3D)environment in vitro,sharing similar structures with their source organs or tissues.Observing themorphology or growth characteristics of organoids through a microscope is a commonly used method of organoid analysis.However,it is difficult,time-consuming,and inaccurate to screen and analyze organoids only manually,a problem which cannot be easily solved with traditional technology.Artificial intelligence(AI)technology has proven to be effective in many biological and medical research fields,especially in the analysis of single-cell or hematoxylin/eosin stained tissue slices.When used to analyze organoids,AI should also provide more efficient,quantitative,accurate,and fast solutions.In this review,we will first briefly outline the application areas of organoids and then discuss the shortcomings of traditional organoid measurement and analysis methods.Secondly,we will summarize the development from machine learning to deep learning and the advantages of the latter,and then describe how to utilize a convolutional neural network to solve the challenges in organoid observation and analysis.Finally,we will discuss the limitations of current AI used in organoid research,as well as opportunities and future research directions.展开更多
The quantity and survival time of astrocytes,which were differentiated from adult adipose-derived stromal cells after exposure to an inducer containing 3-isobutyl-1-methylxanthine,have thus far been unsatisfactory.The...The quantity and survival time of astrocytes,which were differentiated from adult adipose-derived stromal cells after exposure to an inducer containing 3-isobutyl-1-methylxanthine,have thus far been unsatisfactory.The present study investigated the growth and differentiation characteristics of induced astrocytes by observing their growth curves.After induction for 48 hours with an inducer containing 0.5% ethanol,some adult adult adipose-derived stromal cells displayed typical astrocytic morphology.The cell quantity gradually decreased with prolonged induction time.Nestin,glial fibrillary acidic protein,and S-100 expression reached peak levels at 14 days,but neuron-specific enolase was not expressed.These results suggest that the induced astrocytes reached their peak at 14 days.Further optimization of the culture environment may yield mature astrocytes with normal functions,in greater quantity,and prolonged survival time.展开更多
基金Supported by the National"the 13th Five-Year"Key R&D Program(2016YFD0701905)。
文摘Arugula(Eruca sativa Mill.),as an edible medicinal vegetable of peculiar flavor,is served as uncooked dish.The influence of variable lighting intensity(LI)on the growth characteristics and nutritional quality of hydroponically grown arugula was investigated by using light-emitting diodes(LEDs)to light the hydroponically grown arugula for a reference for industrialized arugula production.The dynamic demands of arugula for LI in the seedling stage,initial growth stage and vigorous growth stage were tested under two light quality conditions including a red/blue light ratio of 7:1 and a light/dark photoperiod of 12 h/12 h.Then,the curves of variable LI-induced changes in the growth indices of arugula in different development periods were drawn.Next,the influence of variable LI on the growth characteristics and nutritional quality of arugula was investigated by measuring the dry/fresh weight ratio,chlorophyll content,vitamin C content and soluble protein content.Variable LI significantly increased the height,stem diameter,leaf width,dry/fresh weight ratio,chlorophyll content and soluble protein content of arugula plant.Plant height,stem diameter,dry/fresh weight ratio,chlorophyll content and soluble protein content were the highest in the group exposed to LI of 200,300 and 300μmol•m^(-2)•s^(-1)during the seedling stage,initial growth stage and vigorous growth stage,respectively.The greatest leaf width was achieved at LI of 100,250 and 350μmol•m^(-2)•s^(-1),respectively.High intensity LI markedly repressed the synthesis of vitamin C.
基金the National High-Tech Research and Development Program of China(Grant No.2002AA2Z4331)for generous financial support
文摘A long-term field experiment (started at 2003) was conducted to determine the effects of different dce cultivation methods on growth characteristics and grain yield of late-season rice under double-rice cropping system in seasonal drought region of southeast China (Yujiang County, Jiangxi Province). The rice cultivation methods included no-tillage and flooded rice cultivation (N-F), no-tillage and non-flooded rice cultivation with straw mulching (N-SM), and no-tillage and non-flooded rice cultivation without straw mulching (N-ZM). There was no significant difference in rice grain yield between the N-SM and N-F treatments. However, the rice grain yields in the N-SM and N-F treatments were significantly higher than that in the N-ZM treatment. The late-season rice plants in the N-SM treatment had significantly higher numbers of effective panicles and total grains per hill compared with those in the N-ZM treatment. The above-ground dry matter of late-season rice was similar between the N-SM and N-F treatments. Compared with the N-F treatment, the N-ZM and N-SM treatments significantly decreased the leaf area at the heading stage. Moreover, the N-SM treatment could significantly increase total root length and root tip number at the grain-filling stage compared with the N-ZM treatment.
基金Supported by Yantai Scientific and Technological Development Program(2015YD014)
文摘[Objectives] This study was conducted to discuss the possibility of applying microbial fertilizer to production of facility tomato,so as to determine rational application rate for facility tomato. [Methods]A field experiment was carried out to investigate the effects of the application of microbial fertilizer onto acidic soil on yield,quality and soil chemical properties of facility tomato. [Results] Yield of facility tomato was improved after the application of microbial fertilizer.Treatment ABA-2 showed the highest yield,which was higher than the CK by 7. 98%. On the basis of conventional fertilization,the combined application with microbial fertilizer could promote growth and development of tomato,and could significantly improve fruit weight,and Vc,soluble solid and lycopene contents. Furthermore,the microbial fertilizer could remarkably improve soil p H,alkali-hydrolyzale nitrogen,available phosphorus and rapidly available potassium contents.[Conclusions]The application of microbial fertilizer at 80 kg/667 m;has better effects on tomato growth and development and soil status.
基金supported by a research Grant from Yeungnam University in 2015the National Research Foundation of Korea Grant(NRF No.2016M3C1B6929073)funded by the Ministry of Science,ICT and Future Planning of the Republic of Korea
文摘We estimated the growth volume of artificially reforested Pinus densiflora in a post-fire area on three different contour conditions by comparing and analyzing the vertical and horizontal distributions of below-ground roots on each contour. The main roots at the south-facing slope (SS) developed in a long and straight form, and those on the north-facing slope (NS) in a twisted form. The side roots developed more than the main roots on the Ridge. The depth of taproots decreased in the following order: SS?>?NS?>?Ridge. The roots on the SS developed in a pile-form root structure whereas those in the Ridge and NS developed concentrically near the root collar. The amount of root development decreased in the following order: SS?>?NS?>?Ridge. The ratio of fine roots from the whole-root development decreased in the following order: Ridge?>?SS?>?NS. These results can guide considerations of growth differences according to the planting contour conditions for future establishment of P. densiflora artificial plantations.
基金the National Natural Science Foundation of China(No.31772062)Gannan Camellia Industry Development and Innovative Center Open Fund(Grant No.YK201610).
文摘The growth characteristics of Aspergillus parasitic us incubated on two culture media were ex-amined using shortwave infrared(SWIR,1000-2500 nm)hyperspectral imaging(HSI)in this work.HSI images of the A.parasiticus colonies growing on rose bengal medium(RBM)and maize agar medium(MAM)were recorded daily for 6 days.The growth phases of A.parasiticus were indicated through the pixel number and average spectra of colonies.On score plot of the first principal component(PC1)and PC2,four growth zones with varying mycelium densities were identified.Eight characteristic wavelengths(1095,1145,1195,1279,1442,1655,1834 and 1929 nm)were selected from PC1 loading,average spectra of each colony as well as each growth zone.F urthermore,support vector machine(S VM)classifier based on the eight wavelengths was built,and the classification accuracies for the four zones(from outer to inner zones)on the colonies on RBM were 99.77%,9935%,99.75%and 99.60%and 99.77%,9939%,99.31%and 98.22%for colonies on MAM.In addition,a new score plot of PC2 and PC3 was used to differ-entiate the colonies incubated on RBM and MAM for 6 days.Then characteristic wavelengths of 1067,1195,1279,1369,1459,1694,1834 and 1929 nm were selected from the loading of PC2 and PCg.Based on them,a new SVM model was developed to diferentiate colonies on RBM and MAM with accuracy of 100.00%and 9999%,respectively.In conclusion,SWIR hyperspectral image is a powerful tool for evaluation of growth characteristics of A.parasiticus incubated in diferent culture media.
基金Chongqing Technology Innovation and Application Development Special Project(Grant No.cstc2021jscx-gksbX0064,CSTB2023TIAD-KPX0040,cstc2019 jscx-gksbX0092)National Key R&D Program(Grant No.2021YFD1601103)of China.
文摘The tea plant is a valuable and evergreen crop that is extensively cultivated in China and many other countries.Currently,there is growing research interest in this plant.For the tea industry,it is crucial to develop rapid and non-invasive methods to evaluate tea plants in their natural environment.This article provides a comprehensive overview of non-invasive sensing techniques used for in-situ detection of tea plants.The topics covered include leaf,canopy,and field-level assessments,as well as statistical analysis techniques and characteristics specific to the research.Non-invasive testing technology is primarily used for monitoring and predicting tea pests and diseases,monitoring quality,and nutrients,determining tenderness and grade,identifying tea plant varieties,automatically detecting,and identifying tea buds,monitoring tea plant growth,and extracting tea garden areas through remote sensing.It also helps to evaluate planting suitability,assess disasters,and estimate yields.Additionally,the article examines the challenges and prospects of emerging techniques aimed at resolving the in-situ detection problem for tea plants.It can assist researchers and producers in comprehensively understanding the tea environment,quality characteristics,and growth process,thereby enhancing tea production quality,and fostering tea industry development.
文摘The use of titanium dioxide nanoparticles (nTiO<sub>2</sub>) is gaining interest in agriculture because of their impact on many aspects of plant growth. The present study examines the effects of nTiO<sub>2</sub> (5 nm and 10 nm) applied to seeds and the seedlings as a foliar application on various aspects of growth characteristics and biomass accumulation in lettuce (Lactuca sativa, cv. Grand Rapids). Application of 10 nm nTiO<sub>2</sub> to seeds through imbibition resulted in a significant reduction in shoot biomass accumulation while 5 nm nTiO<sub>2</sub> did not affect the biomass accumulation in lettuce. The application of 10 nm nTiO<sub>2</sub> reduced the fresh shoot biomass accumulation by about 18% compared to the control plants. Other growth characteristics such as shoot dry biomass, root fresh and dry biomass, plant height, and leaf area were not affected by the application of both 5 nm and 10 nm nTiO<sub>2</sub>. In addition, foliar application of these nanoparticles to the lettuce seedlings did not have a significant effect on most of the growth parameters examined, and the increasing concentration ranging from 5 nm/L to 400 mg/L did not produce a consistent response in lettuce. Thus, nTiO<sub>2</sub> application to lettuce seeds had a notable negative impact on shoot growth while foliar application did not have a significant effect on many plant growth characteristics. However, foliar applications produced some symptoms of toxicity to the foliage in the form of necrotic or chlorotic patches on the leaves, which were more pronounced with increasing concentrations of both 5 nm and 10 nm nTiO<sub>2</sub>. However, these symptoms were apparent at a concentration as low as 50 mg/L of nTiO<sub>2</sub>. Thus, foliar application of nTiO<sub>2</sub> may not have a significant impact on many of the growth characteristics in lettuce, but it can result in foliar toxicity.
基金This research was financially supported by the National Natural Science Foundation of China (No. 11502282), the China Scholarship Council Fund (No. 201506425040), the Natural Science Foundation of Jiangsu Province (No. BK20140178).
文摘Under harsh conditions (such as high temperature, high pressure, and millisecond lifetime chemical reaction), a long-standing challenge remains to accurately predict the growth characteristics of nanosize spherical particles and to determine the rapid chemical reaction flow field characteristics, The growth characteristics of similar spherical oxide nanoparticles are further studied by successfully introducing the space-time conservation element-solution element (CE/SE) algorithm with the monodisperse Kruis model. This approach overcomes the nanosize particle rapid growth limit set and successfully captures the characteristics of the rapid gaseous chemical reaction process. The results show that this approach quantitatively captures the characteristics of the rapid chemical reaction, nanosize particle growth and size distribution. To reveal the growth mechanism for numerous types of oxide nanoparticles, it is very important to choose a rational numerical method and particle physics model.
基金support provided by the National Natural Science Foundation of China(Grant No.U1503302,U1603241)the Tianshan Talent Project(Phase III)of the Xinjiang Uygur Autonomous Region and the Local People’s Government of the Xinjiang Uygur Autonomous Region in China sent abroad to study abroad as a complete set of projects(Grant No.L06).
文摘Xinjiang is the province with the largest saline-alkali land in China.The growth of halophytes will be stressed when their salt content reaches the threshold.In this study,the pot experiments were conducted and Suaeda salsa,a typical halophyte,was selected as the object to explore the relationship between growth characteristics and salt content under different soil salinity gradients.Salinity gradients were set according to the maximum soil salinity in the current years of Ebinur Lake Wetland National Nature Reserve(ELWNNR).They were classified using the following threshold values:≤2 g/kg(Control Group),10 g/kg,20 g/kg,30 g/kg,and 40 g/kg.The original spectrum,trilateral position and growth characteristics were used as indexes for analysis.With the increase of soil salt content,the red edge shift occurs first,followed by the blue edge shift.The position of yellow edge,green peak and blue edge were not sensitive to the change of soil salinity.This indicates that the red edge is the best indicator for evaluating plant growth.Compared with the Control Group,when the salt content is less than 10 g/kg,the growth status of plant seems not significantly affected.However,plant growth begins to be stressed when the salt content increases to 20 g/kg,which is a turning point for plant health.The increase of soil salt content can inhibit chlorophyll synthesis and plant growth.Plants begin to die when the salt content reaches 40 g/kg.Therefore,40 g/kg could be regarded as the critical point of salt content which inhibits plant growth.The study also shows that the relationship between plant-height change rate and soil salt content is the most significant,indicating that the plant-height change rate is significantly impacted by soil salt content.
基金funded and supported by Fundamental Research Funds of the Chinese Academy of Forestry,No.CAFYBB2017MB10.
文摘Michelia macclurei Dandy is a significant tree species that has extensive cultivation for forestry and horticulture purposes in southern China,owing to its economic and practical importance.Light quality influences plantlet growth and development during tissue culture.However,the growth characteristic and molecular regulation of M.macclurei under different light quality conditions are not well understood yet.In this study,we investigated the morphological,chlorophyll content,and transcriptomic responses of M.macclurei plantlets under different light-emitting diode(LED)qualities,including white,blue,and red light.The results showed that blue light significantly increased plant height(21.29%)and leaf number(18.65%),while red light decreased plant height and leaf number by 7.53%and 16.49%,respectively.In addition,the plantlets’chlorophyll content and etiolation rate were significantly reduced by blue and red light quality compared to white light.Compared to white light,blue light had a negative effect,leading to decreased rooting rate(64.28%),root number(72.72%),and root length(75.86%).Conversely,red light had a positive effect,resulting in increased rooting rate(24.99%),root number(109.58%),and root length(72.72%).Transcriptome analysis identified 54 differentially expressed genes(DEGs)in three groups that consisted of blue light vs.white light(BL-vs-WL),red light vs.white light(RL-vs-WL),and red light vs.blue light(RL-vs-BL).Specifically,21,7,and 41 DEGs were identified in the three groups,respectively.The DEGs found in the RL-vs-WL and BL-vs-WL groups were involved in plant hormone signaling,nitrogen metabolism,and phenylpropanoid biosynthesis pathways,which suggests that M.macclurei plantlets adapt to the changes of light quality via modulating gene expression.Overall,our study provides valuable insights for understanding the molecular and morphological responses of M.macclurei plantlets under different light qualities.
基金Key Research and Development Program of Hebei Province(19226817D)China Apple Research System(CARS-27)+1 种基金Key Technology R&D Program of Hebei Province(16226312D-2)Basic Research Fund Youth Project of Hebei Academy of Agriculture and Forestry Sciences(2021100102).
文摘[Objectives]The paper was to provide a reference for screening dwarfing rootstock suitable for main spur-type Fuji cultivars in central and southern Hebei Province.[Methods]With spur-type Fuji‘Tianhong 2’as the material,the vegetative growth,yield and fruit quality of 8 different rootstock-scion combinations were compared.[Results]‘Tianhong 2’/SH6 as self-rooted rootstock had large average single fruit weight(256.33 g),large number of fruits per plant(188.68),the highest yield[(3250.08±23.42)kg/667 m ^(2)]and the highest colored area(93.5%),and the soluble solid content reached the requirement of high quality fruit(15.78%).[Conclusions]In central and southern Hebei Province,‘Tianhong 2’grafted on SH6 self-rooted rootstock has moderate growth,high yield and good fruit quality,so it can be considered as the preferred rootstock-scion combination in the local area.
文摘By using single rice cultivation as a control, the effect of rice-fish culture on growth dynamic, plant type and yield formation of rice was studied. The results showed as follows: rice-fish culture improved the physical-chemical properties of arable layer soil of paddy field, extended growth period of rice, increased dry matter and LAI of different growth stages, improved three top leaves area, deterred the degeneration of leaves function, increased the diameter of stem, promoted the growth of roots and the formation of roots in the extended stem. At the same time, rice-fish culture extended the length of basal internodes, increased the number of internodes, uplifted the gravity of plant, and depressed the root vigor. For the grain yield and yield structure office, rice-fish culture decreased ear/tillering ratio, spikelet/panicle and seed set percentage, increased grain weight. If variety choice and cultivation technology were controlled appropriately, rice-fish culture could increase the effective panicles and improve grain yield of rice.
文摘Based on a series of polyploid indica-japonica hybrid plant lines obtained from a new breeding strategy of using double predominance of wide cross and polyploidization to breed super flee, two polyploid indica-japonica hybrids, PSR073 and PSR120 were studied in their growth periods to show the powerful heterosis in a larger scale and to study the characteristics of polyploid indica-japonica hybrids more elaborately. The leaf age, tiller growth, flowering habits, and agronomic traits of them were observed to analyze their growth habits and characteristics. The results showed that the.agronomic traits of PSR073 and PSR120, such as the plant height, panicle length, grain length, grain width, and 1000-grain weight, all acquired obvious predominance of polyploidy, and that the seed setting rate was more than 83%. No significant difference was observed between the two tetraploids and common diploids in the leaf age, tiller growth, and flowering habits. It was concluded that the characteristics of the two powerful heterosis polyploid hybrids were different from those of the polyploid rice reported earlier. Wide cross and polyploidization had no negative effects on their growth habits and characteristics; on the contrary these had powerful heterosis. This had provided theoretic and practical evidences for their application to agricultural production.
基金funded by the National Natural Science Foundation of China(51969003)the Key Research and Development Project of Gansu Province(22YF7NA110)+4 种基金the Discipline Team Construction Project of Gansu Agricultural Universitythe Gansu Agricultural University Youth Mentor Support Fund Project(GAU-QDFC-2022-22)the Innovation Fund Project of Higher Education in Gansu Province(2022B-101)the Research Team Construction Project of College of Water Conservancy and Hydropower Engineering,Gansu Agricultural University(Gaucwky-01)the Gansu Water Science Experimental Research and Technology Extension Program(22GSLK023)。
文摘Wolfberry(Lycium barbarum L.)is important for health care and ecological protection.However,it faces problems of low productivity and resource utilization during planting.Exploring reasonable models for water and nitrogen management is important for solving these problems.Based on field trials in 2021 and 2022,this study analyzed the effects of controlling soil water and nitrogen application levels on wolfberry height,stem diameter,crown width,yield,and water(WUE)and nitrogen use efficiency(NUE).The upper and lower limits of soil water were controlled by the percentage of soil water content to field water capacity(θ_(f)),and four water levels,i.e.,adequate irrigation(W0,75%-85%θ_(f)),mild water deficit(W1,65%-75%θ_(f)),moderate water deficit(W2,55%-65%θ_(f)),and severe water deficit(W3,45%-55%θ_(f))were used,and three nitrogen application levels,i.e.,no nitrogen(N0,0 kg/hm^(2)),low nitrogen(N1,150 kg/hm^(2)),medium nitrogen(N2,300 kg/hm^(2)),and high nitrogen(N3,450 kg/hm^(2))were implied.The results showed that irrigation and nitrogen application significantly affected plant height,stem diameter,and crown width of wolfberry at different growth stages(P<0.01),and their maximum values were observed in W1N2,W0N2,and W1N3 treatments.Dry weight per plant and yield of wolfberry first increased and then decreased with increasing nitrogen application under the same water treatment.Dry weight per hundred grains and dry weight percentage increased with increasing nitrogen application under W0 treatment.However,under other water treatments,the values first increased and then decreased with increasing nitrogen application.Yield and its component of wolfberry first increased and then decreased as water deficit increased under the same nitrogen treatment.Irrigation water use efficiency(IWUE,8.46 kg/(hm^(2)·mm)),WUE(6.83 kg/(hm^(2)·mm)),partial factor productivity of nitrogen(PFPN,2.56 kg/kg),and NUE(14.29 kg/kg)reached their highest values in W2N2,W1N2,W1N2,and W1N1 treatments.Results of principal component analysis(PCA)showed that yield,WUE,and NUE were better in W1N2 treatment,making it a suitable water and nitrogen management mode for the irrigation area of the Yellow River in the Gansu Province,China and similar planting areas.
基金supported by the Special Fund for Agro-scientific Research in the Public Interest, China (20130310104)the Key Technology R&D Program of Tianjin, China (12ZCZDNC09600)
文摘Here, the ozone-treated domestic sludge was diluted up to four different multiples and utilized as a nutritional source for hydroponic lettuce growth. Additionally, lettuce was cultured using the modified Hoagland nutrient solution as a control. The effects of ozone-treated domestic sludge on lettuce growth and nutrition were studied. Results showed that the lettuce treated with modified Hoagland inorganic nutrient solution had increased leaf number, plant height, fresh weight and dry weight compared to those treated with the ozone-treated domestic sludge dilution (P〈0.05). However, the lettuce cultivated with the 2-fold ozone-treated sludge dilution showed significantly higher (P〈0.05) contents of chlorophyll, soluble sugar and ascorbic acid (Vc) compared to that treated with modified Hoagland nutrient solution. And the nitrate concentration in the lettuce cultured with the 2-fold ozone-treated sludge dilution was 53.93% less than that cultured with the modified Hoagland nutrient solution, which was a significant improvement (P〈0.05). This study suggested that the 2-fold ozone-treated sludge dilution is optimal for lettuce hydroponic nutrient requirements.
基金Supported by National Natural Science Foundation of China(31060269)Science and Technology Planning Project of the Inner Mongolia Autonomous Region(20110710)+2 种基金Doctor Station Fund of Ministry of Education(20101515110005)Program of Research and Innovation for Graduate Students in Inner Mongolia(B20151012904Z)Science Research Project of Colleges and Universities in the Inner Mongolia Autonomous Region(NJZY060,NJZC17068)
文摘Taking Cucurbita maxima and Cucurbita moschata as root stocks,and‘Jinyou No 3'cucumber as scion,the effects of different root zone temperature conditions optimal temperature( CK)( 18-20 ℃),suboptimal temperature( 13-15 ℃) and low temperature( 8-10 ℃) on the growth and photosynthesis indexes were studied. The results showed that,compared with optimal temperature( CK),suboptimal temperature and low temperature produced a significant inhibition of growth on cucumbers. The plant height,stem diameter,leaf area,number of leaves and dry weight of aboveground part were all reduced,dry weight of underground part and root shoot ratio all increased,while the inhibition was more significant at low temperature. Low and suboptimal temperature conditions also reduced SPAD value,net photosynthetic rate,transpiration rate,intercellular CO2 concentration and stomatal conductance of the grafted cucumber. And there were differences between different grafted seedlings,and seedlings with‘black seeds' as stock performed better than those with‘white seeds' as stock at low temperature.
基金Supported by National Natural Science Foundation of China (41961054)Second Level Project of Xinjiang Corps Talents & Leading Talents Project of Xinjiang Production and Construction Corps(2019CB018)。
文摘[Objective] The paper was to study the effect of chemical-controlled topping technology on spectral and photosynthetic characteristics of cotton. [Method] Two row spacing configurations and four topping treatments were set in the experiment field, and the spectral and photosynthetic characteristics of cotton under different configurations and topping treatments were analyzed. [Result] There was no significant difference in spectral reflectance of cotton leaves and canopy among all treatments(T1-T8) before topping. After topping, the spectral reflectance of cotton leaves showed little difference in visible light band, but significant differences in near-infrared light band under two row spacing configurations(T2-T4, T6-T8).And in near-infrared band, compared with CK(T1, T5), the reflectance increased and then decreased under close planting with equal row spacing configurations(T2-T4) and narrow row dense planting configurations(T6-T8). However, the change of cotton canopy reflectance in short infrared bands increased first and then decreased under close planting with equal row spacing configuration, and increased constantly under narrow row dense planting configuration. The photosynthetic capacity of leaves increased, and the net photosynthetic rate(A), stomatal conductance(GH_(2O)),transpirationrate(E) and intercellular CO_(2) concentration(Ci) significantly increased by controlling the top with chemicals, and differences were ob-served between two configurations. After topping treatments, the leaf area of apical leaf increased, and chemical-controlled topping treatments had significant difference with the control. The apical cotton leaves were shrunk internally, and the plant height increased gradually by 7-15 cm;the number of fruit branches increased by 2-4, and the area of apical leaves decreased significantly in chemical-controlled topping treatments.[Conclusion] After topping with chemicals, the spectral characteristics of cotton change, the photosynthetic capacity of leaves is improved, and the growth of apical leaves and apex of cotton is inhibited.
基金Supported by Key Research and Development Project of Shandong Province(2016DJ11A07)
文摘[Objectives] A field experiment was carried out with biogas manure replacing partial chemical fertilizer to discuss the effects on growth and development of summer maize and soil environment,so as to provide theoretical basis for efficient utilization and zero growth of chemical fertilizer. [Methods]The summer maize field experiment was carried out in Muping District,Yantai City,with conventional fertilization as control,and sole application of biogas manure,biogas manure + 80% chemical fertilizer and biogas manure + 60% chemical fertilizer as three treatments. Growth indices,yield and yield components of summer maize and soil nutrient utilization status were determined. [Results] On the basis of reduced fertilizer application,the application of biogas manure could significantly promote growth of summer maize,and its plant height,stem diameter,ear position and dry weight per plant significantly increased compared with the CK. Remarkable yield increasing effect was obtained on summer maize,and among the various treatments,treatment BM + CF80 had the highest yield,which was 19. 91% higher than the CK. Furthermore,the combined application of biogas manure and chemical fertilizer not only could improve soil acidity( soil pH was improved by 0. 12 unit),but also could improve soil rapidly available potassium content and reduce soil alkali-hydrolyzable nitrogen content. [Conclusions]Comprehensively,combined application of biogas manure and 80% of chemical fertilizer is the best for growth and development of summer maize and improvement of soil character.
基金the National Key R&D Program of China(No.2017YFA0700500)the National Natural Science Foundation of China(No.62172202)+1 种基金the Experiment Project of ChinaManned Space Program(No.HYZHXM01019)the Fundamental Research Funds for the Central Universities from Southeast University(No.3207032101C3).
文摘In modern terminology,“organoids”refer to cells that grow in a specific three-dimensional(3D)environment in vitro,sharing similar structures with their source organs or tissues.Observing themorphology or growth characteristics of organoids through a microscope is a commonly used method of organoid analysis.However,it is difficult,time-consuming,and inaccurate to screen and analyze organoids only manually,a problem which cannot be easily solved with traditional technology.Artificial intelligence(AI)technology has proven to be effective in many biological and medical research fields,especially in the analysis of single-cell or hematoxylin/eosin stained tissue slices.When used to analyze organoids,AI should also provide more efficient,quantitative,accurate,and fast solutions.In this review,we will first briefly outline the application areas of organoids and then discuss the shortcomings of traditional organoid measurement and analysis methods.Secondly,we will summarize the development from machine learning to deep learning and the advantages of the latter,and then describe how to utilize a convolutional neural network to solve the challenges in organoid observation and analysis.Finally,we will discuss the limitations of current AI used in organoid research,as well as opportunities and future research directions.
文摘The quantity and survival time of astrocytes,which were differentiated from adult adipose-derived stromal cells after exposure to an inducer containing 3-isobutyl-1-methylxanthine,have thus far been unsatisfactory.The present study investigated the growth and differentiation characteristics of induced astrocytes by observing their growth curves.After induction for 48 hours with an inducer containing 0.5% ethanol,some adult adult adipose-derived stromal cells displayed typical astrocytic morphology.The cell quantity gradually decreased with prolonged induction time.Nestin,glial fibrillary acidic protein,and S-100 expression reached peak levels at 14 days,but neuron-specific enolase was not expressed.These results suggest that the induced astrocytes reached their peak at 14 days.Further optimization of the culture environment may yield mature astrocytes with normal functions,in greater quantity,and prolonged survival time.