Experimental Study on Inhibitory Effects of Diallyl Sulfide on Growth and Invasion of Human Osteosarcoma MG-63 Cells

The inhibitory effects of diallyl sulfide(DAS) derived from allicin on in vitro and in vivo proliferation of human osteosarcoma MG-63 cells and the action mechanism,and the influence of DAS on invasive capability of MG-63 cells were investigated in order to search for the novel medicines for osteosarcoma.In the in vitro experiment,MG-63 cells were treated with different concentrations of DSA,and the morphological changes of MG-63 cells were observed under an inverted phase microscope.MTT method was used to assay the proliferation of MG-63 cells.Semi-quantitative reverse transcription polymerase chain reaction(RT-PCR) was used to detect the VEGF mRNA expression level in MG-63 cells.By using Transwell invasion assay,the influence of DAS on invasive ability of MG-63 cells was tested.In the in vivo experiment,the nude mice MG-63 cells tumor-bearing model was established,and different concentrations of DAS were injected beside the tumor.Twenty-one days after treatment,the mice were killed,the tumor size and tumor inhibition rate were calculated.The microvessel density(MVD) was determined by using immunohistochemistry.In the in vitro experiment,different concentrations of DAS could obviously inhibit proliferation of MG-63 cells in a time-and concentration-dependent manner.RT-PCR revealed that the expression levels of VEGF mRNA in DSA groups(different concentrations) were significant reduced as compared with those in control group(all P<0.05).Transwell invasion assay indicated that in 20 and 40 μg/mL DAS groups,the number of migratory cells was 91.4±8.3 and 81.8±7.4 respectively,which was significantly declined as compared with that in control group(150.4±14.7,both P<0.05).In the in vivo experiment,DAS could significantly suppress the growth of MG-63 tumor-bearing tissue.Immunohistochemistry demonstrated that different concentrations(20 and 40 μg/mL) of DAS could significantly decrease MVD of MG-63 tumor-bearing tissue(all P<0.05).It was suggested that DAS could inhibit the growth of MG-63 cells probably by suppressing the expression of VEGF mRNA.

Inhibitory effects of methanolic Olea europaea and acetonic Acacia laeta on growth of Babesia and Theileria

Objective:To evaluate the antipiroplasmic activities of methanolic extract of Olea europaea(MOE)and acetonic extract of Acacia laeta(AAL)against Babesia and Theileria parasites in vitro and evaluate the chemotherapeutic effects of these extracts against Babesia(B.)microti in vivo.Methods:Fluorescence assay using SYBR Green 1 nucleic acid stain was used to detect inhibitory effects of the two extracts as well as the combination effects of the two extracts with diminazene aceturate and atovaquone on four Babesia species and Theileria equi in vitro while for in vivo experiments,8-weekold female BALB/c mice were injected intraperitoneally with 1× 107 B.microti-iRBCs and treated orally at a dose of 150 mg/kg of both extracts.Results:The half maximal inhibitory concentration(IC50)values of AAL against B.bovis,B.bigemina,B.divergens,B.caballi,and Theileria equi were lower than those of MOE extracts.Toxicity assay on Madin-Darby bovine kidney,mouse embryonic fibroblast(NIH/3T3),and human foreskin fibroblast cell lines showed that MOE and AAL affected only the viability of Madin-Darby bovine kidney cell line with half maximal effective concentrations(EC50)of(794.7±41.9)and(873.9±17.5)μg/mL,respectively.The oral treatments of MOE and AAL at 150 mg/kg inhibited the growth of B.microti in mice by 80.4% and 64.4%,respectively.The MOE and diminazene aceturate combination showed a higher chemotherapeutic effect than that of monotherapy.Conclusions:MOE and AAL have the potential to be an alternative remedy for treating piroplasmosis.Furthermore,the combination therapy of MOE + DA was more potent against B.microti infection in mice than their monotherapies.

Aqueous extract of Taxus Chinensis (Pilger) Rehd inhibits lung carcinoma A549 cells through the epidermal growth factor receptor/mitogen-activated protein kinase pathway in vitro and in vivo

OBJECTIVE:To explore the anticancer mechanismof aqueous extract of Taxus Chinensis (Pilger) Rehd (AETC).METHODS: The serum pharmacological method was used to avoid interference from administration of the crude medicinal herbs. Eight purebred New Zealand rabbits were used for preparation of serum containing various concentrations of AETC. Forty-eightBalb/c-nu mice were used for in vivo experiments. The effects of serum containing AETC on the proliferation of A549 cells and expression levels of the epidermal growth factor receptor/mitogen-activated protein kinase(EGFR/MAPK) pathway-related proteins in vitro were investigated. Additionally, the effects on the growth of A549 xenografts in nudemice,and expression levels of the EGFR/MAPK pathway-related proteins in the xenografts,were investigated.RESULTS: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) assay revealed that the serum containing AETC significantly decreased the viability of A549 cells in a dose-dependent manner.Western blot showed that the serum containing various concentrations of AETC strongly reduced the levels of phospho-Jun N-terminal kinase(p-JNK) and phospho-extracellular signal-regulated kinase1/2(ERK1/2) while it increased the level of p-p38. However, no significant effects on the expression levels of JNK, ERK1/2, and p38 MAPK were found. In addition, an anticancer effect from AETC was observed invivo in the Balb/c-nu mice bearing A549xenografts.CONCLUSION: AETC has significant effects on the growth of A549 xenografts and on the activity of the EGFR/MAPK pathway. Therefore, AETC may be beneficial in lung carcinoma treatment.

Effects of insulin-like growth factor binding protein 7 on cell cycle and proliferation of gastric cancer in vitro and in vivo

Objective To investigate the effects of insulin-like growth factor binding protein 7(IGFBP7)on the proliferation,cell cycle of gastric cancer cell and the expression of cynlin D1,cyclin-dependent kinase(CDK)4,and to observe the effects of IGFBP7 on the growth of gastric tumor xenografts in nude mice.Methods The MKN-28cell line was interfered by small interfere ribonucleic acid(siRNA)(interfered group),and blank control group,

黄芩体外抑菌作用研究

目的 :探讨黄芩的体外抑菌作用。方法 :用 K- B纸片扩散法。 1 0 0 %黄芩浸出液滤纸片对大肠杆菌、金黄色葡萄球菌、白色葡萄球菌、绿脓杆菌、乙型链球菌抑菌作用进行了研究。结果 :黄芩对以上细菌均有明显抑菌作用。结论

艾叶挥发油体内外抑菌作用的实验研究

目的检测艾叶挥发油体内外抑菌作用。方法艾叶挥发油的最低抑菌浓度(MIC)检测采用琼脂二倍稀释法,并以红霉素为对照;其致死保护率检测是通过对感染小鼠进行试验性治疗来实现。结果艾叶挥发油对实验菌的MIC90值范围0.78～25μL/mL;对实验菌感染小鼠致死保护率40%～50%。结论艾叶挥发油体外对金黄色葡萄球菌、大肠杆菌、绿脓杆菌具有抑菌作用,其抑菌作用优于或等同于红霉素;体内对金黄色葡萄球菌、大肠杆菌、绿脓杆菌感染小鼠具有较好的保护作用。

蒲公英体外抑菌作用研究

目的 :探讨蒲公英的体外抑菌作用。方法 :用 K- B纸片扩散法 10 0 %蒲公英浸出液滤纸片对金黄色葡萄球菌、变形杆菌、甲型链球菌、乙型链球菌抑菌作用进行了研究。结果 :蒲公英对以上细菌均有明显抑菌作用。结论 :蒲公英在体外有明显的抑菌作用。

连翘体外抑菌作用的研究

目的 :探讨连翘的体外抑菌作用。方法 :用 K- B纸片扩散法。 10 0 %连翘浸出液滤纸片对大肠杆菌、金黄色葡萄球菌、白色葡萄球菌、甲型链球菌、乙型链球菌抑菌作用进行了研究。结果 :连翘对以上细菌均有明显抑菌作用。结论

肉桂体外抑菌作用研究

目的 :探讨肉桂的体外抑菌作用。方法 :用 K-B纸片扩散法 10 0 %肉桂水浸出液滤纸片对大肠杆菌、痢疾杆菌、伤寒杆菌、金黄色葡萄球菌、白色葡萄球菌及白色念珠抑菌作用进行了研究。结果 :肉桂对以上细菌均有明显的抑菌作用。结论 :肉桂在体外有明显的抑菌作用及抗真菌作用。

茶氨酸溴香酰胺对肺癌细胞生长的影响与其作用分子机制的研究

以茶氨酸作为对照,评估现已合成的茶氨酸衍生物茶氨酸溴香酰胺(TBrC)对肺癌细胞生长的抑制作用与其分子机制。采用苔芬兰染色等方法检测不同浓度的TBrC对肺癌细胞和正常细胞生长的影响,运用Hoechst33258荧光染色观察分析有关活性成分对肺癌细胞凋亡的诱导作用,应用蛋白质印迹法检测解析这些肺癌细胞中与凋亡和生长密切相关蛋白的表达和药物可能的作用靶点。此外,通过建立动物肿瘤模型,评价TBrC对荷瘤小鼠肺癌生长的抑制效果。实验结果显示,TBrC抑制肺癌细胞体内外生长的活性超过其母体化合物茶氨酸多倍,对正常细胞和小鼠生长无明显毒性;TBrC显示了诱导肺癌细胞凋亡的作用,其分子机制可能与抑制VEGFR1-Akt-NF-κB信号传导通路相关。本研究结果提示,TBrC具有广泛应用于临床治疗和(或)辅助治疗高转移肺癌和其他癌症的潜力。

山豆根体外抑菌作用研究

目的 :探讨山豆根的体外抑菌作用。方法 :用 K- B纸片扩散法。10 0 %山豆根浸出液滤纸片对大肠杆菌、金黄色葡萄球菌、白色葡萄球菌、甲型链球菌、乙型链球菌抑菌作用进行了研究。结果 :山豆根对以上细菌均有明显抑菌作用。结论 :山豆根在体外有明显的抑菌作用。

苦参体外抑菌作用的研究

目的探讨苦参的体外抑菌作用。方法采用K-B纸片扩散法检测苦参对大肠杆菌、金黄色葡萄球菌、甲型链球菌、乙型链球菌以及变形杆菌的影响。结果与生理盐水组比较,苦参对上述细菌均有明显抑制作用。结论苦参在体外有明显的抑菌作用。

紫草体外抑菌作用研究

目的探讨紫草的体外抑菌作用。方法用K-B纸片扩散法。100%紫草浸出液滤纸片时金黄色葡萄球菌、白色葡萄球菌、绿脓杆菌、大肠杆菌、伤寒杆菌、甲型链球菌、乙型链球菌抑菌作用进行了研究。结果紫草对以上细菌均有明显抑菌作用。结论紫草在体外有明显的抑菌作用。

厚朴体外抑菌作用研究

目的探讨厚朴的体外抑菌作用。方法用K-B纸片扩散法。100%厚朴浸出液滤纸片对金黄色葡萄球菌、白色葡萄球菌、绿脓杆菌、大肠杆菌、伤寒杆菌、甲型链球菌、乙型链球菌抑菌作用进行研究。结果厚朴对以上细菌均有明显抑菌作用。结论厚朴在体外有明显的抑菌作用。

CYP3A4酶介导的人类药物代谢性别差异

药代动力学的性别差异是临床上药物疗效的重要影响因素。引起药代动力学性别差异的比较重要的分子因素包括药物代谢酶和药物转运体等。CYP3A4是人体含量最丰富的酶,具有广泛的代谢底物。大部分体内、外研究表明CYP3A4底物的代谢具有明显的性别差异,女性体内的代谢快于男性。该文系统综述人肝脏CYP3A4酶介导的药物代谢性别差异的研究进展。

茶氨酸溴香酰胺对人肝癌细胞体内外生长的抑制作用

以L-茶氨酸作对照,评估本实验室合成的新颖的茶氨酸衍生物-茶氨酸溴香酰胺(TBrC)对人肝癌HepG2细胞系体内外生长的抑制作用,并初步探究其作用的分子机制。采用MTT法检测不同浓度的TBrC对HepG2细胞体外生长的影响,应用蛋白质印迹法检测解析HepG2细胞中与癌细胞凋亡和生长密切相关蛋白的表达和药物可能作用的分子靶点。此外,建立动物肿瘤模型,与对照组茶氨酸和临床常用抗癌药物五氟尿嘧啶组相比较,评价TBrC对荷瘤裸鼠人肝癌HepG2肿瘤生长的抑制效果。实验结果显示,TBrC抑制人肝癌细胞体内外生长的活性超过其母体化合物茶氨酸分别为3倍和4倍以上,对小鼠生长无明显毒性。TBrC比茶氨酸更显著地抑制肝癌细胞生长因子受体c-Met和抗凋亡的Bcl-2等蛋白的表达;此外,TBrC大大上调促进凋亡的Bax蛋白的表达。TBrC抑制c-Met信号传导通路,下调Bcl-2/Bax蛋白比率可能是其作用的分子机制之一。这些结果提示,TBrC具有广泛应用于临床治疗和(或)辅助治疗人肝癌和其他癌症的潜力。

甘草的体外抑菌作用研究

目的 :探讨甘草的体外抑菌作用。方法 :用 K- B纸片扩散法。 10 0 %甘草浸出液滤纸片对大肠杆菌、金黄色葡萄球菌、白色葡萄球菌、绿脓杆菌、乙型链球菌抑菌作用进行了研究。结果 :甘草对以上细菌均有明显抑菌作用。结论

茶氨酸溴香酰胺对人宫颈癌细胞体内外生长的抑制作用

将茶氨酸为母体合成的茶氨酸衍生物茶氨酸溴香酰胺(TBrC)与茶氨酸作比较,评估茶氨酸溴香酰胺对人宫颈癌细胞体内外生长的抑制作用与其分子机制。应用MTT等方法检测不同浓度的TBrC对人宫颈癌细胞体外生长的影响,运用蛋白质印迹法检测解析这些人宫颈癌细胞中与凋亡和生长密切相关蛋白的表达和药物可能的作用靶点。此外,通过建立动物肿瘤模型,评价TBrC对荷瘤裸鼠人宫颈癌生长的抑制效果。实验结果显示,TBrC抑制人宫颈癌细胞体内外生长的活性超过其母体化合物茶氨酸多倍,对小鼠生长无明显毒性;TBrC作用的分子机制之一可能与抑制VEGFR1-Bcl-2/Bax信号传导通路相关。研究结果提示,TBrC具有广泛应用于临床治疗和(或)辅助治疗人宫颈癌和其他癌症的潜力。

知母体外抑菌作用研究

目的探讨知母的体外抑菌作用。方法用K—B纸片扩散法。采用100%知母浸出液滤纸片对金黄色葡萄球菌、白色葡萄球菌、绿脓杆菌、大肠杆菌、伤寒杆菌、甲型链球菌、乙型链球菌抑菌作用进行了研究。结果知母对以上细菌均有明显抑菌作用。结论知母在体外有明显的抑菌作用。

土茯苓体外抑菌作用研究

目的探讨土茯苓的体外抑菌作用。方法用K-B纸片扩散法。100%土茯苓浸出液滤纸片对金黄色葡萄球菌、白色葡萄球菌、绿脓杆菌、大肠杆菌、伤寒杆菌、甲型链球菌、乙型链球菌抑菌作用进行了研究。结果土茯苓对以上细菌均有明显抑菌作用。结论土茯苓在体外有明显的抑菌作用。