Stiffness-tunable biomaterials provide a good extracellular matrix environment for axon growth and regeneration

Neuronal growth, extension, branching, and formation of neural networks are markedly influenced by the extracellular matrix—a complex network composed of proteins and carbohydrates secreted by cells. In addition to providing physical support for cells, the extracellular matrix also conveys critical mechanical stiffness cues. During the development of the nervous system, extracellular matrix stiffness plays a central role in guiding neuronal growth, particularly in the context of axonal extension, which is crucial for the formation of neural networks. In neural tissue engineering, manipulation of biomaterial stiffness is a promising strategy to provide a permissive environment for the repair and regeneration of injured nervous tissue. Recent research has fine-tuned synthetic biomaterials to fabricate scaffolds that closely replicate the stiffness profiles observed in the nervous system. In this review, we highlight the molecular mechanisms by which extracellular matrix stiffness regulates axonal growth and regeneration. We highlight the progress made in the development of stiffness-tunable biomaterials to emulate in vivo extracellular matrix environments, with an emphasis on their application in neural repair and regeneration, along with a discussion of the current limitations and future prospects. The exploration and optimization of the stiffness-tunable biomaterials has the potential to markedly advance the development of neural tissue engineering.

Telencephalic stab wound injury induces regenerative angiogenesis and neurogenesis in zebrafish:unveiling the role of vascular endothelial growth factor signaling and microglia

After brain damage,regenerative angiogenesis and neurogenesis have been shown to occur simultaneously in mammals,suggesting a close link between these processes.However,the mechanisms by which these processes interact are not well understood.In this work,we aimed to study the correlation between angiogenesis and neurogenesis after a telencephalic stab wound injury.To this end,we used zebrafish as a relevant model of neuroplasticity and brain repair mechanisms.First,using the Tg(fli1:EGFP×mpeg1.1:mCherry)zebrafish line,which enables visualization of blood vessels and microglia respectively,we analyzed regenerative angiogenesis from 1 to 21 days post-lesion.In parallel,we monitored brain cell proliferation in neurogenic niches localized in the ventricular zone by using immunohistochemistry.We found that after brain damage,the blood vessel area and width as well as expression of the fli1 transgene and vascular endothelial growth factor(vegfaa and vegfbb)were increased.At the same time,neural stem cell proliferation was also increased,peaking between 3 and 5 days post-lesion in a manner similar to angiogenesis,along with the recruitment of microglia.Then,through pharmacological manipulation by injecting an anti-angiogenic drug(Tivozanib)or Vegf at the lesion site,we demonstrated that blocking or activating Vegf signaling modulated both angiogenic and neurogenic processes,as well as microglial recruitment.Finally,we showed that inhibition of microglia by clodronate-containing liposome injection or dexamethasone treatment impairs regenerative neurogenesis,as previously described,as well as injury-induced angiogenesis.In conclusion,we have described regenerative angiogenesis in zebrafish for the first time and have highlighted the role of inflammation in this process.In addition,we have shown that both angiogenesis and neurogenesis are involved in brain repair and that microglia and inflammation-dependent mechanisms activated by Vegf signaling are important contributors to these processes.This study paves the way for a better understanding of the effect of Vegf on microglia and for studies aimed at promoting angiogenesis to improve brain plasticity after brain injury.

Transforming growth factor-beta 1 enhances discharge activity of cortical neurons

Transforming growth factor-beta 1(TGF-β1)has been extensively studied for its pleiotropic effects on central nervous system diseases.The neuroprotective or neurotoxic effects of TGF-β1 in specific brain areas may depend on the pathological process and cell types involved.Voltage-gated sodium channels(VGSCs)are essential ion channels for the generation of action potentials in neurons,and are involved in various neuroexcitation-related diseases.However,the effects of TGF-β1 on the functional properties of VGSCs and firing properties in cortical neurons remain unclear.In this study,we investigated the effects of TGF-β1 on VGSC function and firing properties in primary cortical neurons from mice.We found that TGF-β1 increased VGSC current density in a dose-and time-dependent manner,which was attributable to the upregulation of Nav1.3 expression.Increased VGSC current density and Nav1.3 expression were significantly abolished by preincubation with inhibitors of mitogen-activated protein kinase kinase(PD98059),p38 mitogen-activated protein kinase(SB203580),and Jun NH2-terminal kinase 1/2 inhibitor(SP600125).Interestingly,TGF-β1 significantly increased the firing threshold of action potentials but did not change their firing rate in cortical neurons.These findings suggest that TGF-β1 can increase Nav1.3 expression through activation of the ERK1/2-JNK-MAPK pathway,which leads to a decrease in the firing threshold of action potentials in cortical neurons under pathological conditions.Thus,this contributes to the occurrence and progression of neuroexcitatory-related diseases of the central nervous system.

Activation of adult endogenous neurogenesis by a hyaluronic acid collagen gel containing basic fibroblast growth factor promotes remodeling and functional recovery of the injured cerebral cortex

The presence of endogenous neural stem/progenitor cells in the adult mammalian brain suggests that the central nervous system can be repaired and regenerated after injury.However,whether it is possible to stimulate neurogenesis and reconstruct cortical layers II to VI in non-neurogenic regions,such as the cortex,remains unknown.In this study,we implanted a hyaluronic acid collagen gel loaded with basic fibroblast growth factor into the motor cortex immediately following traumatic injury.Our findings reveal that this gel effectively stimulated the proliferation and migration of endogenous neural stem/progenitor cells,as well as their differentiation into mature and functionally integrated neurons.Importantly,these new neurons reconstructed the architecture of cortical layers II to VI,integrated into the existing neural circuitry,and ultimately led to improved brain function.These findings offer novel insight into potential clinical treatments for traumatic cerebral cortex injuries.

Finite Element Analysis on Plane Stress Crack Growth in a Power-Law Hardening Material

Aim To determine numerically the field characteristics in the vied at the tip of a place crack growing steadily in a power-law hardening material. Meteods. Methods on the Euler mode and small-scale yield assumption, the numerical results were given by nonlinear finite element analysis. Results The numerical results of the shape of the active plastic sone, the angular distribution of stresseses and Clack tip opening displacement (CTOD) in the vicinity at the hp of the steadily groWing CraCk are determined. Conclusion The comparison between the numerical results given by the present wort and those given by analytic asymptotic analysis shows that the present work reached a very high accuracy.

Study on the Growth Law of 23-year-old Castanopsis fissa Plantation

This paper studies the growth law of 23-year-old Castanopsis fissa planta- tion in Haiming forest farm （Pingguo County, Baise City, Guangxi Province） with the method of stem analysis. The results show that the average DBH, tree height and volume of the 23-year-old C. fissa plantation are 19.4 cm, 20.4 m and 0.326 1 m3 respectively. The fast growing periods of DBH, tree height and volume are the 2^nd- 8^th, 6^th-11^th, 6^th-23^nd year. Annual increment curve and average increment curve of volume haven＇t intersected in the 23 years, which means the 23-year-old C. fissais plantation has not reached the quantitative mature age. The growth equations of DBH, tree height and volume are D=1.713 3T-0.050 6T2＋0.000 7T2-1.942 2, H= 1.925 7T-0.039 8T2-2.489 0, V=-0.010 4T-0.002 4T2-4.084 8e^-0.005 T2＋0.012 7. Ac- cording to the volume regression equation of Castanopsis fissa, it will reach quanti- tative maturity in the 24~ year.

Analysis of Growth Laws of Leaves of Flue-cured Tobacco in Various Periods

The growth laws of leaves of flue-cured tobacco in different positions in various periods were summarized. The results showed that the growth speed of new leaves of flue-cured tobacco increased constantly until their growth was the fastest, and then it reduced irreversibly. The length and width of leaves in each position increased fastest when they grew depending on photosynthates instead of or- ganic compounds from functional leaves. Therefore, it is concluded that the increases of leaf length, width and area, and dry matter, and the peak of photosynthetic rate appear successively during the process of leaves of flue-cured tobacco, and the leaf area correction coefficient of leaves of flue-cured tobacco is related to the shape of leaves, which are affected by leaf position and the variety of flue-cured tobacco. The study can provide scientific reference for further research on the growth laws of leaves of flue-cured tobacco.

The Fractional Power Law of Wind Wave Growth in Deep Water for Short Fetch

Combining the 3/2 power law proposed by Toba with the significant wave energy balance equation for wind waves, wave growth in deep water for short fetch is investigated. It is found that the variations of wave height and period with fetch have the form of power function with fractional exponents 3/8 and 1/4 respectively. Using these exponents in the power functions and through data fitting, the concise wind wave growth relations for short fetch are obtained.

The Quantitative Law of Network Information Growth

Based on the assumptions of ＂information transfer＂ and ＂information creation＂, this paper educes the multiplied growth mechanism of network information： that the gross quantity of network information （Im） is about n times as much as the quantity of real network information （Ir）. According to this theoretical model, we give a uniform explanation to all kinds of information growth models in existence, and put forward some proposals, such as ＂forbidding information transfer＂ and ＂building up the central information base＂, to control the repeated information flooding on the network and facilitate the full use of network information.

Study on Growth Laws of Marine Science and Technology Talents Based on the Perspective of Big Data

Based on the perspective of big data,the growth characteristics of marine science and technology talents were analyzed,and the growth of marine science and technology talents was divided into five periods:study period,adaptation period,growth period,promotion period and stability period.Moreover,some suggestions for the training of marine science and technology talents were proposed from the aspects of students,families,schools and society.

Study on Growth and Decline Law of Contagious Pleuro-pneumonia in Piglets

In order to establish the immune procedure for Actinobacillus pleuropneumonia and to determine the date for the first immunization on piglets, the growth and decline law of A. pleuropneumonia material antibodies in the piglets borne by cows inoculated with A. pleuropneumonia vaccines （type I, II, and VII） before delivery was detected. The results showed that type I, II, and VII maternal antibodies in piglets decreased gradually with the age growing overall, and was at the critical protection value at the ages of 42-50 days （type I） and 28 days （type VII）, lower than the quantification rate; and the antibodies all turned to be negative until the ages of 70 days （type I）, 60 days （type VII） and 35 days （type II）. The first immunization should be carried out at the age of 42-50 days using type I A. pleuropneumonia vaccine, and at the age of 28 days using type VII A. pleuropneumonia vaccine. However, type II A. pleuropneumonia maternal antibody had lower level and positive rate and could not well protect piglets, so the various A. pleuropneumonia vaccines differed in the date for the first immunization. In order to achieve a better immunization effect, A. pleuropneumonia vaccines with different valences should be further researched and developed.

基于FP-growth算法的交通事故数据关联规则挖掘研究

为了探寻多种事故影响因素共同作用下诱发交通事故的某种规律以及各因素间的关系,本文采用FP-growth算法对收集到的交通事故数据进行分析研究,挖掘其中的潜在价值信息,找出事故发生的原因,根据分析结果给相关部门提出建议,帮助城市交通管理者制定更有效的管理措施,以达到降低交通事故发生频率的目的。

The Evolutive Law concerning Relationship between Economic Growth and Farmers' Income Increase in Xinjiang since the Reform and Opening-up:An Empirical Analysis of the VEC Model

We select the per capita gross domestic product and rural residents' per capita net income in Xinjiang as the indicator variables to measure economic growth and farmers' income increase in Xinjiang, respectively. We establish the cointegration equation, the vector error correction (VEC) model, and use the impulse response function to conduct empirical analysis of the evolutive law concerning relationship between economic growth and farmers' income increase in Xinjiang during the period 1978-2010. The results show that there is a cointegration relationship between economic growth in Xinjiang and farmers' income increase; the former plays an important role in promoting the latter, following the evolutive law "first intensify, then abate, intensify again, and finally become stable". We put the following policy recommendations: providing preferential policies for farmers, scientifically and rationally guiding the transfer of rural labor; improving the function of agriculture, increasing the proportion of non-farm income; gradually bridging the widening income gap between urban and rural areas, coordinating urban and rural development.

基于FP-growth的老年行人交通事故损伤致因研究

为研究老年行人交通事故相关因素及其对损伤程度的影响,以中国交通事故深度调查数据库中2013—2023年710起机动车-老年行人事故为分析对象,利用FP-growth算法挖掘出4类影响因素28个类型变量共5594项关联规则。研究结果表明:行人年龄、碰撞速度、事故时间段及事故地点是影响老年行人事故死亡率的显著因素。特别是,行人年龄和碰撞速度对死亡率有显著影响,随着碰撞速度的增加,死亡率显著上升;60岁及以上老年行人年龄每增加1岁,其死亡率提升0.037倍。此外,事故发生在夜间、郊区或村庄的死亡率更高。研究结果可为提高老年行人交通安全,制定相关安全措施提供一定参考。

STRONG LAW OF LARGE NUMBERS AND GROWTH RATE FOR NOD SEQUENCES

In the paper,we get the precise results of Hájek-Rényi type inequalities for the partial sums of negatively orthant dependent sequences,which improve the results of Theorem 3.1and Corollary 3.2 in Kim(2006)and the strong law of large numbers and strong growth rate for negatively orthant dependent sequences.

ASYMPTOTIC ANALYSIS OF MODE Ⅱ STATIONARY GROWTH CRACK ON ELASTIC-ELASTIC POWER LAW CREEPING BIMATERIAL INTERFACE

A mechanical model was established for modeⅡinterfacial crack static growing along an elastic_elastic power law creeping bimaterial interface. For two kinds of boundary conditions on crack faces, traction free and frictional contact, asymptotic solutions of the stress and strain near tip_crack were given. Results derived indicate that the stress and strain have the same singularity, there is not the oscillatory singularity in the field; the creep power_hardening index n and the ratio of Young's module notably influence the crack_tip field in region of elastic power law creeping material and n only influences distribution of stresses and strains in region of elastic material. When n is bigger, the creeping deformation is dominant and stress fields become steady,which does not change with n. Poisson's ratio does not affect the distributing of the crack_tip field.

Effects of thinning on the understory light environment of different stands and the photosynthetic performance and growth of the reforestation species Phoebe bournei

Light levels determine regeneration in stands and a key concern is how to regulate the light environment of different stand types to the requirements of the understory.In this study,we selected three stands typical in south China(a Cryptomeria japonica plantation,a Quercus acutissima plantation,and a mixed stand of both)and three thinning intensities to determine the best understory light environ-ment for 3-year-old Phoebe bournei seedlings.The canopy structure,understory light environment,and photosynthe-sis and growth indicators were assessed following thin-ning.Thinning improved canopy structure and understory light availability of each stand;species composition was the reason for differences in the understory light environ-ment.Under the same thinning intensity,the mixed stand had the greatest light radiation and most balanced spectral composition.P.bournei photosynthesis and growth were closely related to the light environment;all three stands required heavy thinning to create an effective and sustained understory light environment.In a suitable understory light environment,the efficiency of light interception,absorption,and use by seedlings was enhanced,resulting in a higher carbon assimilation the main limiting factor was stomatal conductance.As a shade-avoidance signal,red/far-red radia-tion is a critical factor driving changes in photosynthesis and growth of P.bournei seedlings,and a reduction increased light absorption and use capacity and height:diameter ratios.The growth advantage transformed from diameter to height,enabling seedlings to access more light.Our findings suggest that the regeneration of shade-tolerant species such as P.bournei could be enhanced if a targeted approach to thinning based on stand type was adopted.

Analysis on Growth and Development Laws of Different Mink Varieties

This study was conducted to investigate the growth and development laws of different mink varieties. The weights of six mink varieties were determined at different growth and development stages, respectively. The growth curves were drawn, and the differences in 150-day-old weight were analyzed. The results showed that the weight of each mink variety grew rapidly after weaning to adult weight; the weight of sliver blue mink was significantly higher than those of imported regal white mink and imported palomino mink; and there were no significant differences between other varieties.

GbLMI1 over-expression improves cotton aboveground vegetative growth

Leaves are the main organ for photosynthesis and organic synthesis in cotton.Leaf shape has important effects on photosynthetic efficiency and canopy formation,thereby affecting cotton yield.Previous studies have shown that LMI1(LATE MERISTEM IDENTITY1)is the main gene regulating leaf shape.In this study,the LMI1 gene was inserted into the 35S promoter expression vector,and cotton plants overexpressing LMI1(OE)were obtained through genetic transformation.Statistical analysis of the biological traits of the T_(1) and T_(2) populations showed that compared to the wild type(WT),OE plants had significantly larger leaves,thicker stems and significantly greater dry weight.Furthermore,plant sections of the main vein and petiole showed that the numbers of cells in those tissues of OE plants were significantly greater.In addition,RNA-seq analysis revealed the differential expression of genes related to gibberellin synthesis and NAC gene family(genes containing the NAC domain)between the OE and WT plants,suggesting that LMI1 is involved in secondary wall formation and cell proliferation,which promotes stem thickening.Moreover,Gene Ontology(GO)analysis revealed enrichment in the terms of calcium ion binding,and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis showed enrichment in the terms of fatty acid degradation,phosphatidylinositol signal transduction system,and c AMP(cyclic adenosine monophosphate)signal pathway.These results suggested that LMI1 OE plants are responsive to gibberellin hormone signals,and have altered messenger signals(c AMP,Ca^(2+))which amplify this function,to promote stronger aboveground vegetative growth.This study found the LMI1 greatly increased the vegetative growth in cotton,which is the basic requirement for higher yield.

The Entropy Law and the Impossibility of Perpetual Economic Growth

Every production-recycling iteration accumulates an inevitable proportion of its matter-energy in the environment, lest the production process itself will be a system in perpetual motion, violating the second law of Thermodynamics. Such high-entropy matter depletes finite stocks of ecosystem services provided by the ecosphere, hence is incompatible with the long-term growth in the material scale of the economic process. Moreover, the complex natural systems governing such stocks respond to depletion by possibly sudden environmental transitions, thus hindering markets' very ability to adapt to the new equilibrium conditions. Consequently, uncertainty of critical resilience thresholds constrains material economic growth.