Bacterial Exofactors Modulate Biofilm Growth and Resistivity to Antimicrobial Drugs

Some bacteria have the ability to co-exist, proliferate and survive in a multicellular community, biofilm. Each participating bacteria can form its colonies and encases itself by a self-produced insoluble extracellular matrix substance (EPS). Microcolonies within biofilm are held together by interactions and bonding of the substances present in the EPS with their separation from the water channels. Similar to insoluble EPS, bacterial microcolonies release soluble exofactors that have direct impacts on the survivability, growth and antibacterial resistivity of other microcolonies made of single- or multi-species bacteria in the same biofilm. How the exofactors of microcolonies of one-type bacteria impact on microcolonies of other-type bacteria is still unclear. We studied about the role of exofactors released from Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa, which are common biofilm-forming pathogenic bacteria. Exofactors facilitate to transform the microenvironment where bacteria can acquire alternative lifestyle with a long survival period and resistivity to certain antimicrobial drugs.

Expression and significance of angiostatin, vascular endothelial growth factor and matrix metalloproteinase-9 in brain tissue of diabetic rats with ischemia reperfusion

Objective: To discuss the expression and significance of angiostatin, vascular endothelial growth factor and matrix metalloproteinase-9 in the brain tissue of diabetic rats with ischemia reperfusion. Methods: A total of 60 male Wistar rats were randomly divided into the normal group, sham group, diabetic cerebral infarction group and single cerebral infarction group according to the random number table, with 15 rats in each group. The high sucrose diet and intraperitoneal injection of streptozotocin were performed for the modeling of diabetic rats, while the thread-occlusion method was employed to build the model of cerebral ischemia reperfusion. The immunohistochemical staining was performed to detect the expression of angiostatin, vascular endothelial growth factor(VEGF) and matrix metalloproteinase-9(MMP-9) in the brain tissue. Results: The expression of angiostatin after the reperfusion in the brain tissue of rats in the single cerebral infarction group and diabetic cerebral infarction group was increased 6 h after the reperfusion, reached to the peak on 1 d and then decreased gradually. The expression of angiostatin in the diabetic cerebral infarction group 6 h, 1 d, 3 d and 7 d after the reperfusion was significantly higher than that in the single cerebral infarction group(P<0.05). VEGF began to be increased 1 h after the reperfusion in the single cerebral infarction group and diabetic cerebral infarction group, reached to the peak at 6 h and then decreased gradually. The expression of VEGF in the diabetic cerebral infarction group at each time point after the reperfusion was significantly lower than that in the single cerebral infarction group(P<0.05). MMP-9 began to be be increased 1 h after the reperfusion in the single cerebral infarction group and diabetic cerebral infarction group, reached to the peak on 1 d and then decreased gradually. The expression of MMP-9 in the diabetic cerebral infarction group at each time point after the reperfusion was significantly higher than that in the single cerebral infarction group(P<0.05). Conclusions: The high glucose environment in which the diabetic cerebral infarction is occurred is to induce the formation of MMP-9 at first and then activate and increase the expression of angiostatin. Afterwards, the expression of VEGF is inhibited, resulting in the poor angiogenesis after cerebral infarction, which thus makes the injury of brain tissue after cerebral infarction even worse than the non-diabetes mellitus.

Vascular Endothelial Growth Factorl65-regulated Nasopharyngeal Carcinoma Cell Lines Invasion and Migration Involve Expression and Activation of Matrix Metalloproteinase-2

The effect of vascular endothelial growth factor （VEGF） overexpression on matrix metalloproteinase-2 （MMP-2） in nasopharyngeal carcinoma （NPC） cells in vitro and the possible mechanism involved were investigated, and the correlation between the expression of VEGF and MMP-2 in NPC evaluated. The NPC cells were transfected with PAd-trackVEGF165 plasmid. The expression levels of VEGF and MMP-2 mRNA and protein in NPC cells were detected by semi-quantitative RT-PCR and Western blot respectively. It was found that the expression of VEGF and MMP-2 mRNA and protein was significantly increased in NPC cells after transfection of VEGF 165. It was concluded that the expression of VEGF was correlated to the in vitro invasion of NPC cells, and the induction of MMP-2 by VEGF was a key process of NPC cell invasion.

Local inhibition of matrix metalloproteinases reduced M2 macrophage activity and impeded recovery in spinal cord transected rats after treatment with fibroblast growth factor-1 and nerve grafts

Alternatively activated macrophages （M2 macrophages） promote central nervous system regeneration. Our previous study demonstrated that treatment with peripheral nerve grafts and fibroblast growth factor-1 recruited more M2 macrophages and improved partial functional recovery in spinal cord transected rats. The migration of macrophages is matrix metalloproteinase （MMP） dependent. We used a general inhibitor of MMPs to influence macrophage migration, and we examined the migration of macrophage populations and changes in spinal function. Rat spinal cords were completely transected at Ts, and 5 mm of spinal cord was removed （group T）. In group R, spinal cord-transected rats received treatment with fibroblast grow th factor- 1 and peripheral nerve grafts. In group RG, rats received the same treatment as group R with the addition of 200 μM GM6001 （an MMP inhibitor） to the fibrin mix. We found that MMP-9, but not MMP- 2, was upregulated in the graft area of rats in group R. Local application of the MMP inhibitor resulted in a reduction in the ratio of arginase-1 （M2 macrophage subset）/inducible nitric oxide synthase-postive cells. When the MMP inhibitor was applied at 8 weeks postoperation, the partial functional recovery observed in group R was lost. This effect was accompanied by a decrease in brain-derived neurotrophic factor levels in the nerve graft. These results suggested that the arginase-1 positive population in spinal cord transected rats is a migratory cell population rather than the phenotypic conversion of early iNOS^＋ cells and that the migration of the arginase-1^＋ population could be regulated locally. Simultaneous application of MMP in- hibitors or promotion of MMP activity for spinal cord injury needs to be considered if the coadministered treatment involves M2 recruitment.

MG63 Osteoblast-like Cells Growth Behaviour on Carbon/Carbon Composites with Different Carbon Matrixes

During the process that implant materials are used for bone replacement,the cell responses to implant materials determine the long-term stability of bone replacement.The microstructure of implant materials is considered as a critical factor that influences the cell responses.Carbon/Carbon composites（C/C composites） are novel implant materials,but there are few reports on the effect of their microstructure,especially the carbon matrixes and holes,on cell behavior.In this paper,C/C composites with different carbon matrixes are prepared by chemical vapor infiltration and pressure impregnation carbonization technique,respectively.The structure of holes is analyzed.The cell responses to C/C composites with different carbon matrixes are evaluated with MG63 osteoblast-like cells.The morphologies of MG63 osteoblast-like cells on the surface of C/C composites,especially in the holes are assessed by scanning electron microscope,and cell proliferation behavior is evaluated by 3-[4,5-dimethylthiozol-2-yl]-2,5-diphenyltetrazolium bromide（MTT） assay. The results show that MG63 osteoblast-like cells have a lamellar morphology with similar sizes and spreading areas as well as the same proliferation behaviors for C/C composites with different carbon matrixes.Carbon matrix shows unapparent influence on the cell growth behavior.Besides,MG63 osteoblast-like cells have various interactions with the holes of C/C composites.The cells stride over the holes with 6～8μm in size,and connect with each other or grow along the curvature wall of the holes with a size of 30-40μm;the cells present three-dimensional morphologies inside the holes and display circular shapes along the ridge of the holes.Diverse cell-material interactions are found according to the size and position of the holes,which provides theoretical foundation for the microstructure design of clinical C/C composites.

The interaction between epidermal growth factor (EGF) and matrix metalloproteinase induces the development of sweat glands in human fetal skin

Objective:The development of sweat glands is a very complicated biological process involving many factors. In this study, we explore the inter-relationship between epidermal growth factor (EGF), matrix metalloproteinases (MMP-2,MMP-7) and development of sweat glands in human embryos. Furthermore, we hope to elucidate the mechanism(s) underlying the induction of epidermal stem cells into sweat gland cells.

Effects of Heparin on Transforming Growth Factor-β_1 and Extracellular Matrix Components in the Glomeruli of Diabetic Rats

The effects of heparin on the expression of transforming growth factor-β 1 (TGF-β 1) and two extracellular matrix components laminin (LN) and fibronectin (FN) in diabetic rat glomeruli were investigated. Twenty-six rats were randomly divided into control group (C, n=8), diabetic group (D, n=9), and diabetes+heparin group (DH, n=9). After 8-week therapy of heparin (200 U once daily by abdominal injection), TGF-β 1, LN and FN expression in glomeruli was detected by immunohistochemical method. The results showed that the expression levels of TGF-β 1, LN and FN were higher in group D than in group C. It was found that heparin could reduce 24-h urinary albumin excretion and inhibit overexpression of TGF-β 1, LN and FN in glomeruli of diabetic rats. It suggested that the inhibitory effect of heparin on diabetic glomerular sclerosis was at least partly related with the inhibition of TGF-β 1 expression.

EXPRESSION OF MATRIX METALLOPROTEINASE-2 AND VASCULAR ENDOTHELIAL GROWTH FACTOR IN HUMAN GLIOMA AND THEIR RELATION TO THE INVASION OF THE TUMOR

Objective To study the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-2 (MMP-2) in different grade human glioma. To investigate their relation to the pathological grade and invasion of the tumor. Methods The expression of MMP-2 and VEGF were determined by immunohistochemical technique in 48 cases of human glioma and 10 specimens of normal brain tissue. Results The expression levels of MMP-2 and VEGF in human glioma were positively related to tumor grades (P<0.01), and their expressions in the glioma of grade Ⅲ and Ⅳ were significantly different from those in the glioma of grade Ⅰ-Ⅱand normal brain tissue (P<0.01). The expression of MMP-2 was positively correlated to that of VEGF (P<0.01). Conclusion MMP-2 and VEGF were highly expression in human glioma and were positively related to the tumor grades. The synergic interaction of MMP-2 and VEGF promoted the angiogenesis and invasion of human glioma.

信迪利单抗联合贝伐珠单抗治疗不可切除肝细胞肝癌患者效果及其血清VEGF、MMP-9水平变化

目的 探讨信迪利单抗联合贝伐珠单抗治疗不可切除肝细胞肝癌患者效果及其血清血管内皮生长因子(VEGF)、基质金属蛋白酶-9(MMP-9)水平变化。方法 选取2021年1月至2022年12月在周口市中心医院确诊的80例不可切除肝细胞肝癌患者,根据患者治疗方式将患者分为单抗联合组和对照组。对照组患者接受口服甲苯磺酸索拉非尼治疗,纳入36例;单抗联合组接受信迪利单抗联合贝伐珠单抗治疗,纳入44例。记录两组患者治疗4个周期后的病情控制率和客观缓解率,比较治疗前、治疗2个周期后、治疗4个周期后血清VEGF和MMP-9水平变化,比较治疗毒副作用发生情况。随访6~30个月,绘制生存曲线,比较两组患者中位总生存时间(OS)和中位无进展生存时间(PFS)。结果 单抗联合组患者病情控制率和客观缓解率分别为77.27%和25.00%,对照组分别为44.44%和5.56%,差异有统计学意义(P<0.05)。治疗后,单抗联合组患者血清VEGF和MMP-9水平均随着时间降低,且低于对照组,差异有统计学意义(P<0.05)。单抗联合组患者甲状腺功能减退发生率为18.18%,对照组为2.78%,差异有统计学意义(P<0.05);单抗联合组患者脱发和手足综合征发生率均为0,对照组分别为22.22%和27.78%,差异均有统计学意义(P<0.05)。所有患者中位随访时间为18个月,单抗联合组患者中位OS和中位PFS分别为16.9个月和4.8个月,对照组患者中位OS和中位PFS分别为10.5个月和3个月,两组比较差异有统计学意义(P<0.05)。结论 信迪利单抗联合贝伐珠单抗治疗可以较好地控制不可切除肝细胞肝癌患者病情发展,显著降低血清肿瘤相关因子水平,提高生存率,且安全性较好。

EGR-1和MMP-2在早期自然流产患者中的表达及临床意义

目的探讨早期生长反应因子(EGR-1)和基质金属蛋白酶-2(MMP-2)在早期自然流产患者绒毛中的表达及临床意义。方法采用RT-PCR和Westernblot检测20例早期自然流产患者(流产组)和20例正常早孕人工流产(对照组)绒毛组织中EGR-1和MMP-2的表达情况。结果流产组中EGR-1、MMP-2 mRNA和蛋白水平明显低于对照组,差异有统计学意义(P<0.05)。结论EGR-1和MMP-2在早孕绒毛组织中表达异常可能参与早期妊娠失败的发生。

甲磺酸阿帕替尼联合多西他赛二线治疗晚期胃癌的效果及对外周血肿瘤异常蛋白、血管内皮生长因子与基质金属蛋白酶-9的影响

目的探讨甲磺酸阿帕替尼联合多西他赛二线治疗晚期胃癌的效果及对外周血肿瘤异常蛋白、血管内皮生长因子(VEGF)与基质金属蛋白酶-9(MMP-9)的影响。方法选取2020年3月至2022年3月收治的90例晚期胃癌患者为研究对象,随机将其分为对照组与观察组,各45例。对照组采用多西他赛化疗方案,观察组在对照组基础上加甲磺酸阿帕替尼治疗。比较两组的治疗效果。结果观察组的疾病控制率高于对照组(P<0.05)。治疗后,观察组的糖类抗原19-9(CA19-9)、肿瘤特异性生长因子(TSGF)及癌胚抗原(CEA)水平均低于对照组(P<0.05)。治疗后,观察组的VEGF、MMP-9、肿瘤坏死因子-α(TNF-α)及白细胞介素-6(IL-6)水平均低于对照组(P<0.05)。两组的不良反应总发生率比较,差异无统计学意义(P>0.05)。结论甲磺酸阿帕替尼联合多西他赛二线治疗晚期胃癌的效果较好,可对外周血肿瘤异常蛋白有良好的抑制作用,同时还能调节VEGF、MMP-9水平,且不会增加不良反应发生风险,值得应用与推广。

不同类型基质和生长调节剂对橡胶草根扦插生根的影响

创建橡胶草新的无性繁殖技术体系,能为今后橡胶草的良种繁育及有效利用提供新的技术支撑,本研究以当年生橡胶草根部为试验材料,试验方法采用L9(34)正交试验设计,分别对植物生长调节剂的种类、浓度、处理时间以及不同扦插基质对橡胶草根扦插生根的影响进行试验研究。试验结果表明,通过对9种处理进行统计分析,橡胶草根生根率间存在显著差异,其中在8号处理条件下的橡胶草生根率最高,达到88.9%;与清水对照处理相比较,植物生长调节剂可对橡胶草根的扦插生根率达到显著影响,但不同种类的植物生长调节剂,在不同浓度以及处理时间下,对橡胶草根扦插苗不同生长指标的影响在统计学上无显著差异;三种不同类型的扦插基质处理下的橡胶草根生根率差异水平达到了极显著,其中以草炭土:珍珠岩=3:1配比条件下的橡胶草根平均生根率最高,为71.2%;8号处理条件下的隶属函数值最高。由此可得相应结论:扦插基质配比为草炭土:珍珠岩=3:1,植物生长调节剂采用ABT1号生根粉,浓度为300 mg/L,处理时间为0.5 min,是适合橡胶草根扦插的最佳处理组合。

输卵管阻塞性不孕患者血清TGF-β1、IL-2、MMP-9水平及临床意义

目的:分析输卵管阻塞性不孕患者血清转化生长因子-β1(TGF-β1)、白细胞介素-2(IL-2)、基质金属蛋白酶-9(MMP-9)水平变化及临床意义。方法:以本院2022年3月-2023年10月诊治的60例输卵管阻塞性不孕患者为病例组,正常妊娠分娩的女性50例为对照组。测定对比两组血清TGF-β1、IL-2、MMP-9水平,Pearson相关性分析血清指标间相关性,受试者工作特征(ROC)曲线分析血清指标诊断不孕效能,logistic多因素回归分析输卵管阻塞性不孕发生影响因素。结果:病例组术前血清TGF-β1(593.79±148.23 ng/L)、IL-2(3.90±0.75 ng/L)、MMP-9(384.88±86.87 ng/ml)水平均高于对照组(239.42±113.37 ng/L、3.09±0.84 ng/L、178.98±80.85 ng/ml),术后血清TGF-β1、IL-2、MMP-9水平较术前均下降,血清TGF-β1与IL-2、MMP-9,及IL-2与MMP-9均呈正相关(均P<0.05)。ROC曲线分析,诊断不孕TGF-β1曲线下面积(AUC)0.941、敏感度88.3%、特异度94.0%,IL-2 AUC 0.778、敏感度66.7%、特异度84.0%,MMP-9 AUC 0.946、敏感度88.3%、特异度86.0%,3指标联合诊断AUC为0.992,敏感度93.3%、特异度100.0%。logistic回归分析,血清TGF-β1、IL-2、MMP-9异常升高是输卵管阻塞性不孕发发生的危险因素(均P<0.05)。结论:输卵管阻塞性不孕患者血清TGF-β1、IL-2、MMP-9水平均高表达,且是不孕发生的危险因素,3者联合检测对输卵管阻塞性不孕有较高诊断价值。

肝细胞癌患者血清ECM1、MMP-9和VEGF水平的变化及其意义

目的 探究肝细胞癌患者血清细胞外基质蛋白-1(ECM1)、基质金属蛋白酶-9(MMP-9和血管内皮生长因子(VEGF)水平的变化及其意义。方法 对60例肝细胞癌患者进行回顾性分析,患者主要使用甲磺酸阿帕替尼进行治疗,必要时也可联合GEMOX方案肝动脉化疗栓塞术进行治疗。采用酶联免疫吸附法测定患者治疗前后的血清ECM1、VEGF、MMP-9水平。比较患者治疗前后ECM1、MMP-9和VEGF水平;比较不同临床病理特征(年龄、性别、血管侵犯、淋巴结转移、TNM分期、Edmondson分期和肿瘤直径)患者ECM1、MMP-9和VEGF水平。结果 治疗后,患者ECM1、MMP-9以及VEGF水平分别为(135.23±44.58)pg/ml、(421.25±87.56)μg/L和(657.56±54.56)pg/ml,均低于治疗前的(215.56±30.80)pg/ml、(499.56±30.56)μg/L、(798.02±50.79)pg/ml(P<0.05)。不同年龄、性别患者ECM1、MMP-9、VEGF水平比较差异无统计学意义(P>0.05);不同血管侵犯、淋巴结转移、TNM分期、Edmondson分期和肿瘤直径患者ECM1、MMP-9、VEGF水平比较差异具有统计学意义(P<0.05)。结论 ECM1、MMP-9、VEGF三者相互作用可促进肝癌细胞的转移,可根据其水平情况,了解患者肝细胞、肝功能健康状况。

MMP-9与IGF-1在垂体腺瘤患者中的表达及其临床预测价值

目的探究MMP-9与IGF-1在垂体腺瘤患者中的表达及其临床预测价值。方法回顾性分析84例垂体腺瘤患者临床资料,分析不同病理垂体腺瘤患者的血清指标[基质金属蛋白酶9(matrix metalloproteinase 9,MMP-9)、胰岛素样生长因子-1(insulin-like growth factor 1,IGF-1)]水平差异。84例垂体腺瘤患者依据治疗后肿瘤直径大小和转移率分为缓解组(n=40)与未缓解组(n=44),采用受试者工作特征(ROC)曲线分析MMP-9、IGF-1对垂体腺瘤患者预后的评估效能。结果不同肿瘤直径的垂体腺瘤患者MMP-9、IGF-1水平比较无显著性差异(P>0.05);未发生淋巴结转移的患者MMP-9水平明显低于发生转移的患者,而IGF-1水平高于发生转移的患者,差异有统计学意义(P<0.05);临床分期为Ⅰ~Ⅱ期的垂体腺瘤患者MMP-9水平明显低于Ⅲ~Ⅳ期患者,而IGF-1水平高于Ⅲ~Ⅳ期患者,差异有统计学意义(P<0.05)。缓解组患者MMP-9低于同期未缓解组,IGF-1水平明显高于同期未缓解组患者,差异有统计学意义(P<0.05)。ROC曲线结果显示血清中MMP-9与IGF-1的截断值分别为16.61 ng/L、229.06 ng/L,MMP-9预测ROC曲线下面积(AUC)为0.871(95%CI=0.778~0.935),敏感度75.61%,特异度92.50%,IGF-1预测ROC曲线AUC为0.851(95%CI=0.755~0.921),敏感度75.61%,特异度87.50%。结论血清中MMP-9,IGF-1水平与垂体腺瘤患者临床病理特征及预后情况密切相关,可通过早期检测结果进一步优化诊治措施。

子宫肌瘤组织中MMP-2、MLCK、VEGF及IGF-2表达及其临床意义

目的分析基质金属蛋白酶-2(MMP-2)、肌球蛋白轻链激酶(MLCK)、血管内皮生长因子(VEGF)、胰岛素样生长因子-2(IGF-2)在子宫肌瘤组织内的表达及临床意义。方法选取76例子宫肌瘤患者,收集其子宫肌瘤组织及正常子宫组织,采用免疫组织化学法测定MMP-2、MLCK、VEGF及IGF-2表达,统计对比两者的阳性率。结果子宫肌瘤组织中MMP-2、MLCK、VEGF及IGF-2阳性表达率分别为69.74%(53/76)、60.53%(46/76)、81.58%(62/76)、92.11%(70/76),高于正常子宫组织的15.79%(12/76)、10.53%(8/76)、17.11%(13/76)、30.26%(23/76),差异有统计学意义(P<0.05)。结论MMP-2、MLCK、VEGF及IGF-2在子宫肌瘤组织中呈异常的高表达,与疾病的发生与发展存在紧密联系。

大鼠周围神经损伤后外周血内皮祖细胞动员及相关因子含量变化

目的探讨大鼠周围神经损伤(PNI)后外周血内皮祖细胞(EPCs)、碱性成纤维细胞生长因子(bFGF)、血管内皮生长因子(VEGF)及基质金属蛋白酶-9(MMP-9)水平变化及EPCs与其他指标的相关性。方法42只SD大鼠按随机数字表法分为对照组、PNI 1 d组、PNI 3 d组、PNI 5 d组、PNI 7 d组及PNI 14 d组,每组7只,除对照组外其余组均采用钳夹法建立坐骨神经损伤模型。对每组在预定时间点采用活体心脏穿刺法采血;采用Ficoll密度梯度离心法提取单个核细胞,CD34和CD133双阳性细胞标记EPCs,应用流式细胞仪检测各组EPCs数量。采用酶联免疫吸附试验检测各组外周血bFGF、VEGF及MMP-9含量,分析EPCs数量与bFGF、VEGF、MMP-9水平的相关性。结果与对照组相比,PNI 3 d组、PNI 5 d组、PNI 7 d组外周血EPCs数量升高,PNI 3 d组、PNI 5 d组、PNI 7 d组及PNI 14 d组外周血bFGF含量升高,其余各组外周血VEGF含量升高,PNI 5 d组、PNI 7 d组及PNI 14 d组外周血MMP-9含量升高(P<0.05)。PNI 5 d组和PNI 7 d组外周血EPCs数量与血清bFGF水平呈正相关(r分别为0.784和0.788,P<0.05),与血清VEGF水平呈正相关(r分别为0.889和0.852,P<0.05);PNI 5 d组、PNI 7 d组和PNI 14 d组外周血EPCs数量与血清MMP-9水平呈正相关(r分别为0.788、0.852和0.873,P<0.05)。结论EPCs与bFGF、VEGF和MMP-9共同参与了PNI后血供修复的病理生理过程。

创面渗出液VEGF、MMP-13、TIMP-1水平对负压封闭引流术联合人工真皮修复难愈性创面效果的预测价值

目的观察难愈性创面患者创面渗出液血管内皮生长因子(vascular endothelial growth factor,VEGF)、基质金属蛋白酶13(matrix metalloproteinase-13,MMP-13)、金属蛋白酶抑制剂1(tissue inhibitor of matrix metalloproteinases-1,TIMP-1)水平,并分析其对负压封闭引流术(vacuum sealing drainage,VSD)联合人工真皮修复治疗难愈性创面临床效果的预测价值。方法选取难愈性创面患者60例纳入难愈组,普通创面患者60例为对照组。所有入选者均检测创面渗出液VEGF、MMP-13、TIMP-1水平,并计算MMP-13/TIMP-1值。采用VSD联合人工真皮修复治疗难愈性创面患者,观察临床效果,并依据临床效果将其分为有效组与无效组,比较有效组与无效组创面渗出液VEGF、MMP-13、TIMP-1水平与MMP-13/TIMP-1值,采用Logistic回归分析上述指标对VSD联合人工真皮修复治疗难愈性创面患者效果的影响,并绘制受试者工作特征曲线(receiver operating characteristic curve,ROC),分析上述指标对VSD联合人工真皮修复治疗难愈性创面患者效果的预测价值。结果难愈组MMP-13水平及MMP-13/TIMP-1值高于对照组,VEGF、TIMP-1水平低于对照组(P<0.05)。60例难愈性创面患者经VSD联合人工真皮修复治疗有效49例(81.67%),无效11例(18.33%)。无效组MMP-13、MMP-13/TIMP-1值高于有效组,VEGF、TIMP-1水平低于有效组(P<0.05)。Logistic回归分析结果显示,MMP-13(95%CI:1.037~1.165)及MMP-13/TIMP-1值(95%CI:1.410~3.458)是难愈性创面患者治疗效果的危险因素,VEGF(95%CI:0.972~0.995)、TIMP-1(95%CI:0.264~0.756)是保护因素(P<0.05)。点二列相关性分析结果显示,VEGF、TIMP-1水平与难愈性创面患者VSD联合人工真皮修复治疗效果呈正相关(r=0.410、0.448,P<0.05),MMP-13水平、MMP-13/TIMP-1值与其治疗效果呈负相关(r=-0.477、0.570,P<0.05)。绘制ROC曲线,结果显示,VEGF(95%CI:0.643~0.908)、MMP-13(95%CI:0.706~0.986)、TIMP-1(95%CI:0.712~0.943)水平及MMP-13/TIMP-1值(95%CI:0.829~0.981)对难愈性创面患者治疗效果具有一定预测价值(AUC=0.776、0.846、0.827、0.905)。结论创面渗出液VEGF、MMP-13、TIMP-1、MMP-13/TIMP-1值能够对VSD联合人工真皮修复治疗难愈性创面效果产生重要影响,临床可通过测定VEGF、MMP-13、TIMP-1、MMP-13/TIMP-1值早期预测治疗效果。

Effects of transforming growth factor β2 and connective tissue growth factor on induction of epithelial mesenchymal transition and extracellular matrix synthesis in human lens epithelial cells

AIM:To Investigate the effects of transforming growth factorβ2(TGF-β2)and connective tissue growth factor(CTGF)on transdifferentiation of human lens epithelial cells(HLECs)cultured in vitro and synthesis of extracellular matrix(ECM).METHODS:HLECs were treated with TGF-β2(0,0.5,1.0,5,10μg/L)and CTGF(0,15,30,60,100μg/L)for different times(0,24,48,72h)in vitro and the expression ofα-smooth muscle actin(α-SMA),the main component of the extracellular matrix typeⅠcollagen(Col-1)and fibronectin(Fn)were measured by using real-time polymerase chain reaction(PCR)and western-blot.RESULTS:TGF-β2 and CTGF significantly increased expression ofα-SMA mRNA and protein(P【0.05,P【0.001),Fn mRNA and protein(P【0.001),Col-1 mRNA and protein(P【0.001).TGF-β2 could induce HLECs expression of CTGF mRNA and protein in dosedependent manner(P【0.05,P【0.001).TGF-β2 and CTGF could induce HLECs to expressα-SMA,Fn and Col-1 in time-dependent manner.Each time of TGF-β2and CTGF induced HELCs expression ofα-SMA,Fn,Col-1 mRNA and protein was significant increase compared with control(P【0.05,P【0.001).CONCLUSION:TGF-β2 and CTGF could induce HLECs epithelial mesenchymal transition and ECM synthesis.

大丽花扦插繁殖影响因子研究

为研究大丽花扦插影响因子,完善大丽花扦插技术体系,以大丽花"樱花"品种为试验材料,研究了不同扦插时期、不同插穗规格、不同基质、不同生长调节剂及其浓度对扦插生根的影响。研究结果表明,不同扦插时期对扦插生根效果有显著影响,以5月扦插的生根率、生根数和根系效果指数显著好于其他时期;基质种类、生长调节剂种类及浓度对生根效果均有显著影响,以处理6(1000 mg/L IBA+珍珠岩)扦插生根效果最好,其存活率、生根率、生根数、最大根直径和根系效果指数分别为95.00%、80.00%、9.54条、4.25 mm和41.93,均高于其他处理,且生根率、生根数和根系效果指数显著优于其他所有处理;插穗粗度相同时,8 cm长的插穗在多个指标上均显著好于5 cm长的插穗,而长度相同时,4~6 mm粗的插穗在最大根长和最大根直径上显著好于2~4 mm,因此插穗规格以S3(长8 cm粗4~6 mm)扦插生根效果最好。综合而言,以5月份用长8 cm粗4~6 mm的插穗经1000 mg/L IBA处理30 s后在珍珠岩扦插其生根情况最好。