Background: A 21-day experiment was conducted to test the hypothesis that Ca requirements to maximize growth performance expressed as the standardized total tract digestible(STTD) Ca to STTD P ratio is less than 1.40:...Background: A 21-day experiment was conducted to test the hypothesis that Ca requirements to maximize growth performance expressed as the standardized total tract digestible(STTD) Ca to STTD P ratio is less than 1.40:1. The second hypothesis was that increasing dietary Ca increases plasma Ca concentration and downregulates abundance of genes related to Ca absorption(TRPV6, S100 G, and ATP2 B1) in the duodenum, and tight junction proteins(OCLN, CLDN1, and ZO1) in the duodenum and ileum.Methods: Twenty corn-soybean meal diets were formulated using a 4 × 5 factorial design with diets containing 0.16%, 0.33%, 0.42%, or 0.50% STTD P, and 0.14%, 0.29%, 0.44%, 0.59%, or 0.74% STTD Ca. Six hundred and forty pigs(initial weight: 11.1 ± 1.4 kg) were allotted to 20 diets and 5 blocks in a randomized complete block design. On day21, weights of pigs and feed left in feeders were recorded and blood, duodenal tissue, ileal mucosa, and the right femur were collected from 1 pig per pen. Abundance of m RNA was determined in duodenal and ileal tissue via quantitative RT-PCR. Data were analyzed using a response surface model.Results: The predicted maximum ADG(614 g), G:F(0.65), and bone ash(11.68 g) was obtained at STTD Ca:STTD P ratios of 1.39:1, 1.25:1, and 1.66:1, respectively, when STTD P was provided at the requirement(0.33%). If dietary STTD P was below the requirement, increasing dietary Ca resulted in reduced(P < 0.05) ADG and G:F. However, if dietary STTD P was above the requirement, negative effects(P < 0.05) on ADG and G:F of increasing STTD Ca were observed only if dietary STTD Ca exceeded 0.6%. Plasma Ca concentration was positively affected by STTD Ca over the range studied(quadratic, P < 0.01) and negatively affected by increasing STTD P(linear, P < 0.01). There was a linear negative effect(P < 0.05) of STTD Ca on the abundance of S100 G, TRPV6, OCLN, and ZO1 in duodenum, and CLDN and ZO1 in ileum.Conclusions: The STTD Ca:STTD P ratio needed to maximize growth performance of 11-to 25-kg pigs is less than1.40:1, if P is at the estimated requirement. Increasing dietary Ca reduces transcellular absorption of Ca and increases paracellular absorption of Ca.展开更多
Chinese Meishan and Jiangquhai pigs are two of the most prolific pigs in the world, but their growth rate is lower than that of Duroc, Landrace and Pietrain pigs. It is suggested that growth rate is regulated by grow...Chinese Meishan and Jiangquhai pigs are two of the most prolific pigs in the world, but their growth rate is lower than that of Duroc, Landrace and Pietrain pigs. It is suggested that growth rate is regulated by growth hormone. The objective of the current study was to analyze the porcine growth hormone (p GH) gene polymorphisms based on the polymerase chain reaction restriction fragment-length polymorphism method (PCR-RFLP) for three western meat-type breeds (Duroc, Landrace and Pietrain) and two local Chinese pigs (Meishan and Jiangquhai) . Five polymorphic restriction sites were detected with the ApaI, MspI, BspI and HhaI restriction enzymes in two amplified fragments (605 bp, - 119 to + 486; 506 bp, + 206 to + 711).Breed difference was found only in the 506 bp fragment. There was no difference in allelic frequencies of BspIand HhaI restriction sites among the five breeds ( P > 0.05) . Landrace and Meishan pigs lacked allele G3 of Msp I site. The allele G3 frequency of restriction Msp I site of the 506 bp fragment in Pietrain pigs was higher than that in Duroc and Jianquhai pigs (P < 0.001 ). For ApaI site, the Meishan pigs lacked allele G1 i no difference was found in allelic frequencies among Pietrain, Duroc, Landrace and Jiangquhai pigs (P >0.05) . This new and rapid PCR-RFLP typing method is an attractive tool for analysis of porcine growth hormone gene restriction sites. The differences in MspI and ApaI restriction sites may explain the growth difference between the foreign meat-type breeds above mentioned and local Chinese pigs.展开更多
Background:This study was conducted to determine the effects of different dietary protein levels and amino acids supplementation patterns in low protein diets on the growth performance,carcass characteristics and nitr...Background:This study was conducted to determine the effects of different dietary protein levels and amino acids supplementation patterns in low protein diets on the growth performance,carcass characteristics and nitrogen excretion in growing-finishing pigs.Forty-two barrows(25.00±0.39 kg)were randomly assigned to 7 diets.Diet 1:the high crude protein diet with balanced for 10 essential amino acids(EAAs).Diet 2:the medium crude protein diet with 2%(approx)decreased protein level of Diet 1 and balanced 10 EAAs.Diet 3:the low crude protein diet with 4%decreased protein level of Diet 1 and balanced 10 EAAs.The protein levels of Diet 4,5,6 and 7 were the same as that of Diet 3.Diet 4 was only balanced for lysine(Lys),methionine(Met),threonine(Thr)and tryptophan(Trp);Diet 5 and 6 were further supplemented with extra isoleucine(Ile)or valine(Val),respectively;Diet 7 was further supplemented with extra Ile+Val.Results:Over the 112 days trial,the reduction of dietary protein by 2%or 4%with balanced10 EAAs significantly decreased nitrogen excretion(P<0.05),but had no effects on growth performance and carcass characteristics(P>0.05).In low protein diet,Val supplementation significantly increased body weight gain at 25–50 kg phase(P<0.05),while Ile supplementation at 75–100 kg phase and 100–125 kg phase significantly reduced the ratio of feed to gain(P<0.05).No effect of different dietary protein levels and amino acids supplementation patterns in low protein diets on carcass characteristics was observed(P>0.05).The total N excretion of pigs supplemented with only Lys,Met,Thr and Trp was numerically higher than that of pigs fed with extra Ile,or Val,or Ile+Val diets.Conclusion:In low protein diet,Val is more required than Ile in the early growing phage(25–50 kg),while Ile becomes more required in the late growing and finishing phage(75–125 kg).展开更多
A total of 72 growing-finishing pigs(56 days old) were used to investigate the effects of dietary probiotics on growing performance, pork quality and serum metabolites. Using single factorial experiment design, pigs...A total of 72 growing-finishing pigs(56 days old) were used to investigate the effects of dietary probiotics on growing performance, pork quality and serum metabolites. Using single factorial experiment design, pigs were allotted to three groups(four pens/group and six pigs/pen). Diet treatments were a corn-soybean meal(control group) and a corn-soybean meal with 1% probiotics(probiotics group) or 80 mg kg-1colistin sulfate(antibiotics group). Pigs were slaughtered when pigs of the lightest averaged 90 kg(157 days of age). There was no difference(p&gt;0.05) in average daily gain or feed/gain, and the average daily feed intake of the antibiotics group was higher than that of the probiotics and the control groups(p&lt;0.05). Diet supplemented with probiotics reduced the drip loss and cooking loss of pork(p&lt;0.05). Dietary supplementation with probiotics significantly decreased the content of triglycerides, lowdensity lipoprotein and urea nitrogen in serum(p&lt;0.05), and increased the content of high-density lipoprotein in serum(p&lt;0.05). These results suggested improving effects of dietary probiotics on pork quality and serum metabolism in growing-finishing pigs.展开更多
Background: There are various fat sources with different energy values and fatty acid compositions that may affect growth performance and carcass composition of grow-finishing pigs. A higher net energy was recently re...Background: There are various fat sources with different energy values and fatty acid compositions that may affect growth performance and carcass composition of grow-finishing pigs. A higher net energy was recently reported in choice white grease compared with soybean oil. Therefore, two experiments were conducted to determine whether practical responses confirm that difference between choice white grease and soybean oil, and to extend the observations to other fat sources.Results: In Exp. 1, pigs fed fats had lower(P < 0.05) average daily feed intake in phase II and overall period,greater(P < 0.05) gain:feed in phase I, phase II, and overall period than pigs fed the control diet. Pigs fed fats tended(P = 0.057) to have thicker backfat depth at the last rib than those fed control. Pigs fed 6% fats had greater(P < 0.01) gain:feed in phase II and overall period than pigs fed 3% fats. During phase I, pigs fed choice white grease grew faster(P < 0.05) than pigs fed soybean oil. In Exp. 2, pigs fed dietary fats(soybean oil, choice white grease, animal-vegetable blend, palm oil, or tallow) had greater(P < 0.01) gain:feed in each phase and overall period, greater(P < 0.01) average daily gain in phase I, but lower(P < 0.01) average daily feed intake in phase II an overall than pigs fed the control diets. The choice white grease also increased(P < 0.05) average daily gain during phase I compared with soybean oil. Pigs fed palm oil had thicker(P < 0.05) backfat depth at the 10 thrib than those fed soybean oil, animal-vegetable blend, or tallow.Conclusions: Inclusion of 6% dietary fat improved feed efficiency of finishing pigs, while different fats produced different practical results that may be consistent with their different energy values. Results from the early stage indicate that dietary fats with relatively more saturated fatty acids may provide greater energy than those with relatively more unsaturated fatty acids for growing pigs.展开更多
Feed fats and oils provide significant amounts of energy to swine diets, but there is large variation in composition,quality, feeding value, and price among sources. Common measures of lipid quality include moisture, ...Feed fats and oils provide significant amounts of energy to swine diets, but there is large variation in composition,quality, feeding value, and price among sources. Common measures of lipid quality include moisture, insolubles,and unsaponifiables(MIU), titer, and free fatty acid content, but provide limited information regarding their feeding value. Lipid peroxidation is an important quality factor related to animal growth performance and health, but maximum tolerable limits in various lipids have not been established. Several indicative assays can be used to detect the presence of various peroxidation compounds, but due to the complexity and numerous compounds produced and degraded during peroxidation process, no single method can adequately determine the extent of peroxidation. Until further information is available, using a combination of peroxide value, thiobarbituric acid reactive substances(TBARS), and anisidine value appear to provide a reasonable assessment of the extent of peroxidation in a lipid at a reasonable cost. However, fatty acid composition of the lipid being evaluated should be considered when selecting specific assays. Predictive tests can also be used to estimate the stability or susceptibility of lipids to peroxidation and include active oxygen method, oil stability index, and oxygen bomb method. A review of 16 published studies with pigs has shown an average decrease of 11.4% in growth rate, 8.8% feed intake fed isocaloric diets containing peroxidized lipids compared to diets containing unperoxidized lipids of the same source.Furthermore, serum vitamin E content was generally reduced and serum TBARS content was increased when peroxidized lipids were fed in these studies, suggesting that feeding peroxidized lipids negatively affects metabolic oxidative status of pigs. However, it is unclear if antioxidants are useful additions to lipids to maintain optimal nutritional value, or if their addition to swine diets is beneficial in overcoming a metabolic oxidative challenge.展开更多
[Objective] The paper was to study the effects on growth performance and feed digestibility of Hainan black pigs by adding lysine in low prntein diet. [Method] A tntal of 36 Hainan black pigs with the body weight of ...[Objective] The paper was to study the effects on growth performance and feed digestibility of Hainan black pigs by adding lysine in low prntein diet. [Method] A tntal of 36 Hainan black pigs with the body weight of (46.34±3.06) kg were randomly divided into three groups: con- trol group (protein level 15%, lysine level 0.83%), experimental grnup 1 (protein level 13%, 0.83%), and experimental group II (protein level 13%, lysine level 1.03), each group had three replicates of four pigs. The trial lasted 35 d. [Result] The growth performance of Hainan black pig in grnup II had no significan! difference with that in contrail group (P〉0.05). The apparent digestibility of chide protein and phosphorus of Hainan black pigs in Group II increased significantly (P〈0.05), which were increased by 10.08% and 11.85% respectively compared to the control group; the apparent digestibility of calcium was increased, but the difference was not signifieant (P〉0.05). [Conclusion] It will not reduce the growth performance of Hainan blaek pig when the protein level of diet is reduced by 2% and Ihe lysine level is increased to 1.03%. It is recommended that the diet can be used in the production of llainan black pig.展开更多
Background: The protein/energy ratio is important for the production performance and utilization of available feed resources by animals. Increased protein consumption by mammals leads to elevated feed costs and incre...Background: The protein/energy ratio is important for the production performance and utilization of available feed resources by animals. Increased protein consumption by mammals leads to elevated feed costs and increased nitrogen release into the environment. This study aimed to evaluate the effects of dietary protein/energy ratio on the growth performance, carcass traits, meat quality, and plasma metabolites of pigs of different genotypes. Methods: Bama mini-pigs and Landrace pigs were randomly assigned to two dietary treatment groups (Chinese conventional diet with low protein/energy ratio or National Research Council diet with high protein/energy ratio; n = 24 per treatment) in a 2 x 2 factorial arrangement. Blood and muscle samples were collected at the end of the nursery, growing, and finishing phases. Results: We observed significant interactions (P 〈 0.05) between breed and diet for total fat percentage, intramuscular fat (IMF) content, protein content in biceps femoris (BF) muscle, and plasma urea nitrogen (UN) concentration in the nursery phase; for average daily gain (ADG), average daily feed intake (ADFI), dry matter, IMF content in psoas major (PM) muscle, and plasma total protein and albumin concentrations in the growing phase; and for drip loss and plasma UN concentration in the finishing phase. Breed influenced (P 〈 0.05) growth performance, carcass traits, and meat quality, but not plasma metabolites. Throughout the trial, Landrace pigs showed significantly higher (P 〈 0.0_5) ADG, ADFI, dressing percentage, lean mass rate, and loin-eye area than did Bama mini-pigs, but significantly lower (P 〈 0.0.5) feed/gain ratio, fat percentage, backfat thickness, and IMF content. Dietary protein/energy ratio influenced the pH value, chemical composition of BF and PM muscles, and plasma activities of glutamic-pyruvic transaminase and gamma-glutamyl transpeptidase, and plasma concentration of UN. Conclusions: Compared with Landrace pigs, Bama mini-pigs showed slower growth and lower carcass performance, but had better meat quality. Moreover, unlike Landrace pigs, the dietary protein/energy ratio did not affect the growth performance of Bama mini-pigs. These results suggest that, in swine production, low dietary protein/energy ratio may be useful for reducing feed costs and minimizing the adverse effects of ammonia release into the environment.展开更多
Background: Supplementation of feed with long-chain fatty acids(LCFAs) during the grower and finisher phases has long been discussed as a growth promotion strategy in pigs, but its effects are inconsistent. The purpos...Background: Supplementation of feed with long-chain fatty acids(LCFAs) during the grower and finisher phases has long been discussed as a growth promotion strategy in pigs, but its effects are inconsistent. The purpose of our study was to comprehensively evaluate its effects on the growth performance based on the average daily gain(ADG), average daily feed intake(ADFI) and gain: feed(G:F) ratio and to unveil the roles of the basal diet, LCFA concentration and LCFA saturation.Results: We searched the Pub Med and Web of Science databases(articles published from Jan 1 st, 2000, to Sep 30 th,2018;restricted to English) and compared LCFA-supplemented diets with control diets. We retrieved 2346 studies, 18 of which(1314 pigs, 26 records) were eligible for our analysis. We used a random-effects model to calculate the weighted mean differences(WMDs) and 95% confidence intervals(CIs). LCFA supplementation in the grower-finisher phase improved the ADG(WMD = 41.74 g/d, 95% CI: 8.81 to 74.66, P = 0.013) and G:F ratio(WMD = 0.019, 95% CI: 0.006 to 0.032, P = 0.003). For supplementation solely in the finisher phase, we found a similar performance in the ADG(WMD = 39.93 g/d, 95% CI: 26.48 to 53.38, P < 0.001) and G:F ratio(WMD = 0.019, 95% CI: 0.006 to 0.032, P < 0.001) but a reduction in the ADFI(WMD =-83.863 g/d, 95% CI:-156.157 to-11.569, P = 0.023). Specifically, approximately 5%LCFA supplementation in the finisher phase had significant effects on the ADG(WMD = 51.385 g/d, 95% CI: 35.816 to66.954, P < 0.001), ADFI(WMD =-102.869 g/d, 95% CI:-189.236 to-16.502, P = 0.02) and G:F ratio(WMD = 0.028, 95%CI: 0.018 to 0.039, P < 0.001), whereas a concentration of approximately 1% exhibited no effects.Conclusions: Overall, regardless of the basal diet and saturation, LCFA supplementation greatly improves the growth performance of grower and finisher pigs, primarily by increasing the energy density.展开更多
Background: Genomic growth curves are general y defined only in terms of population mean; an alternative approach that has not yet been exploited in genomic analyses of growth curves is the Quantile Regression(QR). Th...Background: Genomic growth curves are general y defined only in terms of population mean; an alternative approach that has not yet been exploited in genomic analyses of growth curves is the Quantile Regression(QR). This methodology allows for the estimation of marker effects at different levels of the variable of interest. We aimed to propose and evaluate a regularized quantile regression for SNP marker effect estimation of pig growth curves, as well as to identify the chromosome regions of the most relevant markers and to estimate the genetic individual weight trajectory over time(genomic growth curve) under different quantiles(levels).Results: The regularized quantile regression(RQR) enabled the discovery, at different levels of interest(quantiles), of the most relevant markers al owing for the identification of QTL regions. We found the same relevant markers simultaneously affecting different growth curve parameters(mature weight and maturity rate): two(ALGA0096701 and ALGA0029483)for RQR(0.2), one(ALGA0096701) for RQR(0.5), and one(ALGA0003761) for RQR(0.8). Three average genomic growth curves were obtained and the behavior was explained by the curve in quantile 0.2, which differed from the others.Conclusions: RQR allowed for the construction of genomic growth curves, which is the key to identifying and selecting the most desirable animals for breeding purposes. Furthermore, the proposed model enabled us to find, at different levels of interest(quantiles), the most relevant markers for each trait(growth curve parameter estimates) and their respective chromosomal positions(identification of new QTL regions for growth curves in pigs). These markers can be exploited under the context of marker assisted selection while aiming to change the shape of pig growth curves.展开更多
Both growth hormone-releasing peptide 6 (GHRP-6) and growth hormone-releasing hormone (GHRH) have potent GH-releasing activity in animals. We have previously demonstrated that the administration of a plasmid encod...Both growth hormone-releasing peptide 6 (GHRP-6) and growth hormone-releasing hormone (GHRH) have potent GH-releasing activity in animals. We have previously demonstrated that the administration of a plasmid encoding the GHRH gene to pregnant mice and pig augmented long-term growth in first generation progeny,and that the administration of GHRP-6 results in growth augmentation in mice and rabbits. However,it has not yet been reported if GHRP-6 induces intergenerational growth effects in pigs. Ploy lactic-co-glycolic acid (PLGA) microsphere adsorption of treatment proteins enhances gene expression,genetic immunization and the ability to protect plasmid DNA and peptides from degradation. The cur-rent study was conducted to determine the growth performance of piglets born to gilts treated with GHRP-6 incorporated into thermosensitive PLGA-PEG-PLGA triblock copolymers. Gilts were injected intra-muscularly once at day 85 of gestation with 30 mg of GHRP6-loaded thermosensitive PLGA-PEG-PLGA triblock copolymers. Piglets were weighed periodically between birth and 28 days. Mean body weights of piglets born to GHRP-6-treated gilts were 6. 58% to 18. 89% (P 〈 0. 05 ) greater than those of piglets born to control gilts. This study confirms that enhanced maternal GHRP-6 mediated by thermosensitive PLGA-PEG-PLGA can augment growth of piglets.展开更多
Background: Limited availability of fish meal and whey protein concentrate increases overall feed costs. Availability of increased number of supplemental amino acids including Lys, Met, Thr, Trp, Val, and lie allows ...Background: Limited availability of fish meal and whey protein concentrate increases overall feed costs. Availability of increased number of supplemental amino acids including Lys, Met, Thr, Trp, Val, and lie allows replacing expensive protein supplements to reduce feed costs. This study was to evaluate the effect of replacing fish meal and/or whey protein concentrate in nursery diets with 6 supplemental amino acids on growth performance and gut health of post-weaning pigs. Treatments were 1) FM-WPC: diet with fish meal (FM) and whey protein concentrate (WPC); 2) FM-AA: diet with FM and crystalline amino acids (L-Lys, L-Thr, L-Trp, DL-Met, L-Val, and L-lie); 3) WPC-AA: diet with WPC and crystalline amino acid; and 4) AA: diet with crystalline amino acid. Results: Pigs in FM-AA, WPC-AA, and AA had greater (P 〈 0.0.5) ADG and gain:feed than pigs in FM-WPC during wk ] (phase 1). Plasma insulin concentration of pigs in AA tended to be greater (P = 0.064) than that of FM-WPC at the end of wk 1 (phase 1). Plasma concentrations of IgG in AA was lower (P 〈 0.05) compared with WPC-AA and FW, and FM-AA had lower (P 〈 0.05) IgG concentration than WPC-AA at the end of wk 1 (phase 1). Concentration of acetate in cecum digesta in FM-AA tended to be greater (P = 0.054) than that of FM-WPC and WPC-AA. Concentration of isovalerate in cecum digesta of pigs in FM-AA was greater (P 〈 0.05) than that of FW and WPC-AA. Conclusions: This study indicates that use of 6 supplemental amino acids can replace fish meal and/or whey protein concentrate without adverse effects on growth performance, immune status, and gut health of pigs at d 21 to 49 of age. Positive response with the use of 6 supplemental amino acids in growth during the first week of post-weaning may due to increased plasma insulin potentially improving uptake of nutrients for protein synthesis and energy utilization. The replacement of fish meal and/or whey protein concentrate with 6 supplemental amino acids could decrease the crude protein level in nursery diets, and potentially lead to substantial cost savings in expensive nursery diets.展开更多
Background: This study was designed to evaluate the efficacy of DL-selenomethionine (DL^SeMet) supplementation on growth performance, antioxidant status, plasma selenium (Se) concentration, and immune function of...Background: This study was designed to evaluate the efficacy of DL-selenomethionine (DL^SeMet) supplementation on growth performance, antioxidant status, plasma selenium (Se) concentration, and immune function of weaning pigs. 216 weaning pigs were randomly allocated to 6 treatments with 6 replicates each according to a complete randomized block design. Each replicate had six pigs. Diet of group one was corn-soybean basal diet without any additional Se supplement. Group 2 was supplemented with 0.3 mg/kg of Se from sodium selenite. Groups 3-6 were supplemented with 0.1, 0.3, 0.5, and 0.7 mg/kg of Se from DL-SeMet, respectively. The trial lasted for 42 days. Results: Pigs supplemented with 0.3 and 0.7 mg/kg DL-SeMet obtained better feed gain ratio (P 〈 0.05). The best antioxidant ability (serum, liver, and muscle) was shown in 0.1-0.3 mg/kg DL-SeMet groups (P〈 0.05). The plasma Se concentration increased as the dietary DL-SeMet level elevated. The immunity among groups was not affected. Conclusions: DL-SeMet supplementation in the diet significantly improved the growth performance, antioxidant ability and plasma Se content of weaning pigs. DL-SeMet can replace sodium selenite in the diet of weaning pigs.展开更多
[Objective] The paper was to determine whether the germplasm resources of Congjiang fragrance pig had changed over the past 30 years. [Method]The growth development and reproductive performance of 100 pigs from six to...[Objective] The paper was to determine whether the germplasm resources of Congjiang fragrance pig had changed over the past 30 years. [Method]The growth development and reproductive performance of 100 pigs from six towns in central fragrance pig producing area and Congjiang fragrance pig stock seed farm were measured,and further compared with the data in Guizhou Livestock and Poultry Breeds 1986. [Result]The growth and development status was basically consistent,and the average litter size was at a downward trend: the first litter decreased by 26. 2%; the second litter decreased by 7. 0%; the third litter decreased by8. 55%. [Conclusion] The study laid the foundation for vigorous promotion of Congjiang fragrance pig in industrial development process,acceleration of breeding pace,gradual updating of farming breeds by farmers and improvement of production efficiency of fragrance pig.展开更多
Two experiments were conducted to investigate the effect of dietary supplementation with phytase transgenic corn (PTC) on growth performance,phosphorus (P) utilization and excretion in growing pigs.In Exp.1,180 pi...Two experiments were conducted to investigate the effect of dietary supplementation with phytase transgenic corn (PTC) on growth performance,phosphorus (P) utilization and excretion in growing pigs.In Exp.1,180 pigs (Large White × Landrace,BW=37.7 kg) were randomly allotted to 4 treatments with 5 replicates of 9 pigs each in order to evaluate the effect of PTC supplementation in low-P diets on growth performance.Four corn soybean meal-based diets consisted of a positive control (PC) diet,a diet containing 500 units (U) of exogenous phytase kg-1 (EP) on the basis of low-P (inorganic P reduced by 0.05% from PC diet) and the low-P+500 (PTC1) or 750 (PTC2) phytase U of PTC kg-1.In Exp.2,20 barrows (Large White×Landrace,BW=31 kg,4 treatments with 5 replicates of 1 pig each) were randomly selected to evaluate the effect of PTC in low-P diets on serum parameters and nutrient utilization.Diets in Exp.2 were similar to those in Exp.1 except that the EP group was replaced by a low-P diet without exogenous phytase supplementation as a negative control (NC) group.The results from Exp.1 showed that the average daily gain (ADG) in the PTC2 group was significantly higher (P〈0.05) than that in the EP group over all periods.On the other hand,the feed:gain (F:G) ratio of the EP group was significantly higher (P〈0.05) than that of the PTC2 group during 1-21 and 1-42 d,respectively.There were no differences in average daily feed intake (ADFI) among all treatments (P〉0.05).The results from Exp.2 showed that the concentration of serum Ca in the NC group was the highest (P〈0.05),while the concentration of serum P in the PTC2 group was the highest (P〈0.05) among all treatments.There was a significant decrease (P〈0.05) in the P apparent digestibility of the NC group compared with the other groups,and that of PTC2 group was the best.Furthermore,fecal P excretion was reduced (P〈0.05) from 1.80 g d-1 in the PC group to 1.28 g d-1 in the PTC2 group.In conclusion,dietary supplementation with PTC could reduce the application of inorganic P,decrease fecal P excretion,and improve the growth performance of growing pigs.展开更多
Both quantitatively and qualitatively, underfeeding materialized by underconsumption of meat remains one of the things most felt in Africa. To make up for this deficit, emphasis is placed on the production of short-cy...Both quantitatively and qualitatively, underfeeding materialized by underconsumption of meat remains one of the things most felt in Africa. To make up for this deficit, emphasis is placed on the production of short-cycle animals such as poultry, rabbits and pork. Among these animals, pig plays a very important socio-economic role in the life of local populations. Its meat is required in various ceremonies such as the dowry and funerals. For this purpose, twenty four males cross piglets with Large White × Landrace × Duroc × Pietrain phenotypic traits, aged between 58 and 62 days and having an average weight of 9.978 ± 1.11 kg were used to evaluate the influence of graded levels of bovine rumen content on the growth performances of grower finisher pigs. The piglets were submitted to 4 treatments, housed in pairs and fed ad libitum with a diet containing 0, 5%, 10% and 20% bovine rumen content. The trial which lasted for 18 weeks was carried out at the Teaching and Research Farm of the University of Dschang. The results showed that drying process destroyed most microbes found in the rumen content. These results also showed that the incorporation of bovine rumen content in diets had no significant (p > 0.05) effect on feed conversion ratio efficiency. However, feed intake, body weight and body weight gain were significantly higher in pigs fed with a diet (R<sub>5</sub>) containing 5% of bovine rumen content. Feed cost per kg of live body weight was significantly (p <sub>20</sub> diet compared to the animals receiving the control diet which was lower than that of the other two diets (R<sub>0</sub>) and (R<sub>5</sub>) but comparable with animals fed diet R<sub>10</sub>. The analysis of the carcass showed that increasing incorporation level of bovine rumen content in the diet reduces abdominal fat and back fat thickness. For offal, the lowest weight was recorded in animals fed the control diet (R<sub>0</sub>) and the highest weight with animals fed a diet containing 5% bovine rumen content. Carcass length of animals fed the control diet (R<sub>0</sub>) was significantly greater compared with the carcass of animals fed diet R<sub>10</sub> and comparable to that of the other two treatments. The pH of the pork was comparable (p > 0.05) in all the treatments. In conclusion, a diet containing 5% bovine rumen content induced the best growth performances compared to all the other groups, while diet R<sub>20</sub> produced lean pork with the higher production cost.展开更多
Ninety finishing pigs were selected to study the effect of N-methyl-aspartate and betaine on the internal growth hormone level in the serum and the correlation between the growth hormone level, growth performance and ...Ninety finishing pigs were selected to study the effect of N-methyl-aspartate and betaine on the internal growth hormone level in the serum and the correlation between the growth hormone level, growth performance and carcass characteristic of finishing pig. The study showed that the two matters could improve pig growth and carcass composition significantly. The correlation analyses indicated that the growth hormone and IGF-I have a positive correlation with the growth rate. Carcass lean ratio, longissimus dorsi area, serum free fatty acid and lipase activity have a negative correlation with the feed conversion ratio, carcass fat ratio and urine nitrogen. But the growth hormone is more effective than IGF-I (P<0.01). The results implicated that both the two matters may act through growth hormone axis (growth hormone - IGF-I) to manipulate pig growth.展开更多
Ten healthy weaning piglets of Landrace x Yorkshire (LY) in good body condition with body weight of (24 ± 1.27) kg were selected, and ten weaning piglets of Berkshire × LY (BLY) and Duroc × LY (...Ten healthy weaning piglets of Landrace x Yorkshire (LY) in good body condition with body weight of (24 ± 1.27) kg were selected, and ten weaning piglets of Berkshire × LY (BLY) and Duroc × LY (DLY) with similar situation were also selected respectively to study the growth performance of different cross combinations. Three groups of experimental pigs were bred with conventional diet. The results showed that the average dry matter intakes of combinations of BLY and DLY were slightly decreased by 4.97% and 5.47% compared with the combination of LY, while average daily gain was significantly increased by 3.13% and 1.14%, and feed-weight ratio was decreased by 7.94% and 6.35%, respectively; economic benefit per pig was increased by 0.61 and 0.41 yuan, respectively. The combinations of BLY and DLY have better growth performance, which can be promoted in the production.展开更多
基金Financial support for this research from AB Vista,Marlborough,UK
文摘Background: A 21-day experiment was conducted to test the hypothesis that Ca requirements to maximize growth performance expressed as the standardized total tract digestible(STTD) Ca to STTD P ratio is less than 1.40:1. The second hypothesis was that increasing dietary Ca increases plasma Ca concentration and downregulates abundance of genes related to Ca absorption(TRPV6, S100 G, and ATP2 B1) in the duodenum, and tight junction proteins(OCLN, CLDN1, and ZO1) in the duodenum and ileum.Methods: Twenty corn-soybean meal diets were formulated using a 4 × 5 factorial design with diets containing 0.16%, 0.33%, 0.42%, or 0.50% STTD P, and 0.14%, 0.29%, 0.44%, 0.59%, or 0.74% STTD Ca. Six hundred and forty pigs(initial weight: 11.1 ± 1.4 kg) were allotted to 20 diets and 5 blocks in a randomized complete block design. On day21, weights of pigs and feed left in feeders were recorded and blood, duodenal tissue, ileal mucosa, and the right femur were collected from 1 pig per pen. Abundance of m RNA was determined in duodenal and ileal tissue via quantitative RT-PCR. Data were analyzed using a response surface model.Results: The predicted maximum ADG(614 g), G:F(0.65), and bone ash(11.68 g) was obtained at STTD Ca:STTD P ratios of 1.39:1, 1.25:1, and 1.66:1, respectively, when STTD P was provided at the requirement(0.33%). If dietary STTD P was below the requirement, increasing dietary Ca resulted in reduced(P < 0.05) ADG and G:F. However, if dietary STTD P was above the requirement, negative effects(P < 0.05) on ADG and G:F of increasing STTD Ca were observed only if dietary STTD Ca exceeded 0.6%. Plasma Ca concentration was positively affected by STTD Ca over the range studied(quadratic, P < 0.01) and negatively affected by increasing STTD P(linear, P < 0.01). There was a linear negative effect(P < 0.05) of STTD Ca on the abundance of S100 G, TRPV6, OCLN, and ZO1 in duodenum, and CLDN and ZO1 in ileum.Conclusions: The STTD Ca:STTD P ratio needed to maximize growth performance of 11-to 25-kg pigs is less than1.40:1, if P is at the estimated requirement. Increasing dietary Ca reduces transcellular absorption of Ca and increases paracellular absorption of Ca.
文摘Chinese Meishan and Jiangquhai pigs are two of the most prolific pigs in the world, but their growth rate is lower than that of Duroc, Landrace and Pietrain pigs. It is suggested that growth rate is regulated by growth hormone. The objective of the current study was to analyze the porcine growth hormone (p GH) gene polymorphisms based on the polymerase chain reaction restriction fragment-length polymorphism method (PCR-RFLP) for three western meat-type breeds (Duroc, Landrace and Pietrain) and two local Chinese pigs (Meishan and Jiangquhai) . Five polymorphic restriction sites were detected with the ApaI, MspI, BspI and HhaI restriction enzymes in two amplified fragments (605 bp, - 119 to + 486; 506 bp, + 206 to + 711).Breed difference was found only in the 506 bp fragment. There was no difference in allelic frequencies of BspIand HhaI restriction sites among the five breeds ( P > 0.05) . Landrace and Meishan pigs lacked allele G3 of Msp I site. The allele G3 frequency of restriction Msp I site of the 506 bp fragment in Pietrain pigs was higher than that in Duroc and Jianquhai pigs (P < 0.001 ). For ApaI site, the Meishan pigs lacked allele G1 i no difference was found in allelic frequencies among Pietrain, Duroc, Landrace and Jiangquhai pigs (P >0.05) . This new and rapid PCR-RFLP typing method is an attractive tool for analysis of porcine growth hormone gene restriction sites. The differences in MspI and ApaI restriction sites may explain the growth difference between the foreign meat-type breeds above mentioned and local Chinese pigs.
基金supported by the Grant from the Science and Technology Support Program of Sichuan Province(2015NZ0042,2016NZ006)National Key R&D Program of China(2018YFD0500605).
文摘Background:This study was conducted to determine the effects of different dietary protein levels and amino acids supplementation patterns in low protein diets on the growth performance,carcass characteristics and nitrogen excretion in growing-finishing pigs.Forty-two barrows(25.00±0.39 kg)were randomly assigned to 7 diets.Diet 1:the high crude protein diet with balanced for 10 essential amino acids(EAAs).Diet 2:the medium crude protein diet with 2%(approx)decreased protein level of Diet 1 and balanced 10 EAAs.Diet 3:the low crude protein diet with 4%decreased protein level of Diet 1 and balanced 10 EAAs.The protein levels of Diet 4,5,6 and 7 were the same as that of Diet 3.Diet 4 was only balanced for lysine(Lys),methionine(Met),threonine(Thr)and tryptophan(Trp);Diet 5 and 6 were further supplemented with extra isoleucine(Ile)or valine(Val),respectively;Diet 7 was further supplemented with extra Ile+Val.Results:Over the 112 days trial,the reduction of dietary protein by 2%or 4%with balanced10 EAAs significantly decreased nitrogen excretion(P<0.05),but had no effects on growth performance and carcass characteristics(P>0.05).In low protein diet,Val supplementation significantly increased body weight gain at 25–50 kg phase(P<0.05),while Ile supplementation at 75–100 kg phase and 100–125 kg phase significantly reduced the ratio of feed to gain(P<0.05).No effect of different dietary protein levels and amino acids supplementation patterns in low protein diets on carcass characteristics was observed(P>0.05).The total N excretion of pigs supplemented with only Lys,Met,Thr and Trp was numerically higher than that of pigs fed with extra Ile,or Val,or Ile+Val diets.Conclusion:In low protein diet,Val is more required than Ile in the early growing phage(25–50 kg),while Ile becomes more required in the late growing and finishing phage(75–125 kg).
基金Supported by China Agriculture Research System(CARS-36)Program for Innovative Research Team of Universities in Heilongjiang Province
文摘A total of 72 growing-finishing pigs(56 days old) were used to investigate the effects of dietary probiotics on growing performance, pork quality and serum metabolites. Using single factorial experiment design, pigs were allotted to three groups(four pens/group and six pigs/pen). Diet treatments were a corn-soybean meal(control group) and a corn-soybean meal with 1% probiotics(probiotics group) or 80 mg kg-1colistin sulfate(antibiotics group). Pigs were slaughtered when pigs of the lightest averaged 90 kg(157 days of age). There was no difference(p&gt;0.05) in average daily gain or feed/gain, and the average daily feed intake of the antibiotics group was higher than that of the probiotics and the control groups(p&lt;0.05). Diet supplemented with probiotics reduced the drip loss and cooking loss of pork(p&lt;0.05). Dietary supplementation with probiotics significantly decreased the content of triglycerides, lowdensity lipoprotein and urea nitrogen in serum(p&lt;0.05), and increased the content of high-density lipoprotein in serum(p&lt;0.05). These results suggested improving effects of dietary probiotics on pork quality and serum metabolism in growing-finishing pigs.
文摘Background: There are various fat sources with different energy values and fatty acid compositions that may affect growth performance and carcass composition of grow-finishing pigs. A higher net energy was recently reported in choice white grease compared with soybean oil. Therefore, two experiments were conducted to determine whether practical responses confirm that difference between choice white grease and soybean oil, and to extend the observations to other fat sources.Results: In Exp. 1, pigs fed fats had lower(P < 0.05) average daily feed intake in phase II and overall period,greater(P < 0.05) gain:feed in phase I, phase II, and overall period than pigs fed the control diet. Pigs fed fats tended(P = 0.057) to have thicker backfat depth at the last rib than those fed control. Pigs fed 6% fats had greater(P < 0.01) gain:feed in phase II and overall period than pigs fed 3% fats. During phase I, pigs fed choice white grease grew faster(P < 0.05) than pigs fed soybean oil. In Exp. 2, pigs fed dietary fats(soybean oil, choice white grease, animal-vegetable blend, palm oil, or tallow) had greater(P < 0.01) gain:feed in each phase and overall period, greater(P < 0.01) average daily gain in phase I, but lower(P < 0.01) average daily feed intake in phase II an overall than pigs fed the control diets. The choice white grease also increased(P < 0.05) average daily gain during phase I compared with soybean oil. Pigs fed palm oil had thicker(P < 0.05) backfat depth at the 10 thrib than those fed soybean oil, animal-vegetable blend, or tallow.Conclusions: Inclusion of 6% dietary fat improved feed efficiency of finishing pigs, while different fats produced different practical results that may be consistent with their different energy values. Results from the early stage indicate that dietary fats with relatively more saturated fatty acids may provide greater energy than those with relatively more unsaturated fatty acids for growing pigs.
文摘Feed fats and oils provide significant amounts of energy to swine diets, but there is large variation in composition,quality, feeding value, and price among sources. Common measures of lipid quality include moisture, insolubles,and unsaponifiables(MIU), titer, and free fatty acid content, but provide limited information regarding their feeding value. Lipid peroxidation is an important quality factor related to animal growth performance and health, but maximum tolerable limits in various lipids have not been established. Several indicative assays can be used to detect the presence of various peroxidation compounds, but due to the complexity and numerous compounds produced and degraded during peroxidation process, no single method can adequately determine the extent of peroxidation. Until further information is available, using a combination of peroxide value, thiobarbituric acid reactive substances(TBARS), and anisidine value appear to provide a reasonable assessment of the extent of peroxidation in a lipid at a reasonable cost. However, fatty acid composition of the lipid being evaluated should be considered when selecting specific assays. Predictive tests can also be used to estimate the stability or susceptibility of lipids to peroxidation and include active oxygen method, oil stability index, and oxygen bomb method. A review of 16 published studies with pigs has shown an average decrease of 11.4% in growth rate, 8.8% feed intake fed isocaloric diets containing peroxidized lipids compared to diets containing unperoxidized lipids of the same source.Furthermore, serum vitamin E content was generally reduced and serum TBARS content was increased when peroxidized lipids were fed in these studies, suggesting that feeding peroxidized lipids negatively affects metabolic oxidative status of pigs. However, it is unclear if antioxidants are useful additions to lipids to maintain optimal nutritional value, or if their addition to swine diets is beneficial in overcoming a metabolic oxidative challenge.
基金Supported by Technical Development Project of Provincial Scientific Research Institutes in Hainan(KYYS-2016-12)
文摘[Objective] The paper was to study the effects on growth performance and feed digestibility of Hainan black pigs by adding lysine in low prntein diet. [Method] A tntal of 36 Hainan black pigs with the body weight of (46.34±3.06) kg were randomly divided into three groups: con- trol group (protein level 15%, lysine level 0.83%), experimental grnup 1 (protein level 13%, 0.83%), and experimental group II (protein level 13%, lysine level 1.03), each group had three replicates of four pigs. The trial lasted 35 d. [Result] The growth performance of Hainan black pig in grnup II had no significan! difference with that in contrail group (P〉0.05). The apparent digestibility of chide protein and phosphorus of Hainan black pigs in Group II increased significantly (P〈0.05), which were increased by 10.08% and 11.85% respectively compared to the control group; the apparent digestibility of calcium was increased, but the difference was not signifieant (P〉0.05). [Conclusion] It will not reduce the growth performance of Hainan blaek pig when the protein level of diet is reduced by 2% and Ihe lysine level is increased to 1.03%. It is recommended that the diet can be used in the production of llainan black pig.
基金supported by the National Basic Research Program of China(No.2012CB124704 and 2013CB127305)KC.Wong Education Foundation,Hong Kong
文摘Background: The protein/energy ratio is important for the production performance and utilization of available feed resources by animals. Increased protein consumption by mammals leads to elevated feed costs and increased nitrogen release into the environment. This study aimed to evaluate the effects of dietary protein/energy ratio on the growth performance, carcass traits, meat quality, and plasma metabolites of pigs of different genotypes. Methods: Bama mini-pigs and Landrace pigs were randomly assigned to two dietary treatment groups (Chinese conventional diet with low protein/energy ratio or National Research Council diet with high protein/energy ratio; n = 24 per treatment) in a 2 x 2 factorial arrangement. Blood and muscle samples were collected at the end of the nursery, growing, and finishing phases. Results: We observed significant interactions (P 〈 0.05) between breed and diet for total fat percentage, intramuscular fat (IMF) content, protein content in biceps femoris (BF) muscle, and plasma urea nitrogen (UN) concentration in the nursery phase; for average daily gain (ADG), average daily feed intake (ADFI), dry matter, IMF content in psoas major (PM) muscle, and plasma total protein and albumin concentrations in the growing phase; and for drip loss and plasma UN concentration in the finishing phase. Breed influenced (P 〈 0.05) growth performance, carcass traits, and meat quality, but not plasma metabolites. Throughout the trial, Landrace pigs showed significantly higher (P 〈 0.0_5) ADG, ADFI, dressing percentage, lean mass rate, and loin-eye area than did Bama mini-pigs, but significantly lower (P 〈 0.0.5) feed/gain ratio, fat percentage, backfat thickness, and IMF content. Dietary protein/energy ratio influenced the pH value, chemical composition of BF and PM muscles, and plasma activities of glutamic-pyruvic transaminase and gamma-glutamyl transpeptidase, and plasma concentration of UN. Conclusions: Compared with Landrace pigs, Bama mini-pigs showed slower growth and lower carcass performance, but had better meat quality. Moreover, unlike Landrace pigs, the dietary protein/energy ratio did not affect the growth performance of Bama mini-pigs. These results suggest that, in swine production, low dietary protein/energy ratio may be useful for reducing feed costs and minimizing the adverse effects of ammonia release into the environment.
基金supported by the Key Program of the National Natural Science Foundation of China(grant#3163000269)National Special Fund for Modern Industrial Technology System(grant#CARS-35)Major Science and Technology Special Fund of Zhejiang Province(grant#2015C02022)
文摘Background: Supplementation of feed with long-chain fatty acids(LCFAs) during the grower and finisher phases has long been discussed as a growth promotion strategy in pigs, but its effects are inconsistent. The purpose of our study was to comprehensively evaluate its effects on the growth performance based on the average daily gain(ADG), average daily feed intake(ADFI) and gain: feed(G:F) ratio and to unveil the roles of the basal diet, LCFA concentration and LCFA saturation.Results: We searched the Pub Med and Web of Science databases(articles published from Jan 1 st, 2000, to Sep 30 th,2018;restricted to English) and compared LCFA-supplemented diets with control diets. We retrieved 2346 studies, 18 of which(1314 pigs, 26 records) were eligible for our analysis. We used a random-effects model to calculate the weighted mean differences(WMDs) and 95% confidence intervals(CIs). LCFA supplementation in the grower-finisher phase improved the ADG(WMD = 41.74 g/d, 95% CI: 8.81 to 74.66, P = 0.013) and G:F ratio(WMD = 0.019, 95% CI: 0.006 to 0.032, P = 0.003). For supplementation solely in the finisher phase, we found a similar performance in the ADG(WMD = 39.93 g/d, 95% CI: 26.48 to 53.38, P < 0.001) and G:F ratio(WMD = 0.019, 95% CI: 0.006 to 0.032, P < 0.001) but a reduction in the ADFI(WMD =-83.863 g/d, 95% CI:-156.157 to-11.569, P = 0.023). Specifically, approximately 5%LCFA supplementation in the finisher phase had significant effects on the ADG(WMD = 51.385 g/d, 95% CI: 35.816 to66.954, P < 0.001), ADFI(WMD =-102.869 g/d, 95% CI:-189.236 to-16.502, P = 0.02) and G:F ratio(WMD = 0.028, 95%CI: 0.018 to 0.039, P < 0.001), whereas a concentration of approximately 1% exhibited no effects.Conclusions: Overall, regardless of the basal diet and saturation, LCFA supplementation greatly improves the growth performance of grower and finisher pigs, primarily by increasing the energy density.
基金supported by Coordination for the Improvement of Higher Education Personnel(Capes)Foundation Arthur Bernardes(Funarbe)Foundation of research Support of the state of Minas Gerais(FAPEMIG)
文摘Background: Genomic growth curves are general y defined only in terms of population mean; an alternative approach that has not yet been exploited in genomic analyses of growth curves is the Quantile Regression(QR). This methodology allows for the estimation of marker effects at different levels of the variable of interest. We aimed to propose and evaluate a regularized quantile regression for SNP marker effect estimation of pig growth curves, as well as to identify the chromosome regions of the most relevant markers and to estimate the genetic individual weight trajectory over time(genomic growth curve) under different quantiles(levels).Results: The regularized quantile regression(RQR) enabled the discovery, at different levels of interest(quantiles), of the most relevant markers al owing for the identification of QTL regions. We found the same relevant markers simultaneously affecting different growth curve parameters(mature weight and maturity rate): two(ALGA0096701 and ALGA0029483)for RQR(0.2), one(ALGA0096701) for RQR(0.5), and one(ALGA0003761) for RQR(0.8). Three average genomic growth curves were obtained and the behavior was explained by the curve in quantile 0.2, which differed from the others.Conclusions: RQR allowed for the construction of genomic growth curves, which is the key to identifying and selecting the most desirable animals for breeding purposes. Furthermore, the proposed model enabled us to find, at different levels of interest(quantiles), the most relevant markers for each trait(growth curve parameter estimates) and their respective chromosomal positions(identification of new QTL regions for growth curves in pigs). These markers can be exploited under the context of marker assisted selection while aiming to change the shape of pig growth curves.
基金supported by grants from the National Natural Science Foundation of China (u0731004, 30771572 )the National Basic Research Program of China (2009CB941601)the Hebei Province Nature Science Foundation (0722553)
文摘Both growth hormone-releasing peptide 6 (GHRP-6) and growth hormone-releasing hormone (GHRH) have potent GH-releasing activity in animals. We have previously demonstrated that the administration of a plasmid encoding the GHRH gene to pregnant mice and pig augmented long-term growth in first generation progeny,and that the administration of GHRP-6 results in growth augmentation in mice and rabbits. However,it has not yet been reported if GHRP-6 induces intergenerational growth effects in pigs. Ploy lactic-co-glycolic acid (PLGA) microsphere adsorption of treatment proteins enhances gene expression,genetic immunization and the ability to protect plasmid DNA and peptides from degradation. The cur-rent study was conducted to determine the growth performance of piglets born to gilts treated with GHRP-6 incorporated into thermosensitive PLGA-PEG-PLGA triblock copolymers. Gilts were injected intra-muscularly once at day 85 of gestation with 30 mg of GHRP6-loaded thermosensitive PLGA-PEG-PLGA triblock copolymers. Piglets were weighed periodically between birth and 28 days. Mean body weights of piglets born to GHRP-6-treated gilts were 6. 58% to 18. 89% (P 〈 0. 05 ) greater than those of piglets born to control gilts. This study confirms that enhanced maternal GHRP-6 mediated by thermosensitive PLGA-PEG-PLGA can augment growth of piglets.
文摘Background: Limited availability of fish meal and whey protein concentrate increases overall feed costs. Availability of increased number of supplemental amino acids including Lys, Met, Thr, Trp, Val, and lie allows replacing expensive protein supplements to reduce feed costs. This study was to evaluate the effect of replacing fish meal and/or whey protein concentrate in nursery diets with 6 supplemental amino acids on growth performance and gut health of post-weaning pigs. Treatments were 1) FM-WPC: diet with fish meal (FM) and whey protein concentrate (WPC); 2) FM-AA: diet with FM and crystalline amino acids (L-Lys, L-Thr, L-Trp, DL-Met, L-Val, and L-lie); 3) WPC-AA: diet with WPC and crystalline amino acid; and 4) AA: diet with crystalline amino acid. Results: Pigs in FM-AA, WPC-AA, and AA had greater (P 〈 0.0.5) ADG and gain:feed than pigs in FM-WPC during wk ] (phase 1). Plasma insulin concentration of pigs in AA tended to be greater (P = 0.064) than that of FM-WPC at the end of wk 1 (phase 1). Plasma concentrations of IgG in AA was lower (P 〈 0.05) compared with WPC-AA and FW, and FM-AA had lower (P 〈 0.05) IgG concentration than WPC-AA at the end of wk 1 (phase 1). Concentration of acetate in cecum digesta in FM-AA tended to be greater (P = 0.054) than that of FM-WPC and WPC-AA. Concentration of isovalerate in cecum digesta of pigs in FM-AA was greater (P 〈 0.05) than that of FW and WPC-AA. Conclusions: This study indicates that use of 6 supplemental amino acids can replace fish meal and/or whey protein concentrate without adverse effects on growth performance, immune status, and gut health of pigs at d 21 to 49 of age. Positive response with the use of 6 supplemental amino acids in growth during the first week of post-weaning may due to increased plasma insulin potentially improving uptake of nutrients for protein synthesis and energy utilization. The replacement of fish meal and/or whey protein concentrate with 6 supplemental amino acids could decrease the crude protein level in nursery diets, and potentially lead to substantial cost savings in expensive nursery diets.
基金supported by NOVUS Intl.(MO.USA)The authors are grateful to the company for supplying fund and DL-SeMet samples
文摘Background: This study was designed to evaluate the efficacy of DL-selenomethionine (DL^SeMet) supplementation on growth performance, antioxidant status, plasma selenium (Se) concentration, and immune function of weaning pigs. 216 weaning pigs were randomly allocated to 6 treatments with 6 replicates each according to a complete randomized block design. Each replicate had six pigs. Diet of group one was corn-soybean basal diet without any additional Se supplement. Group 2 was supplemented with 0.3 mg/kg of Se from sodium selenite. Groups 3-6 were supplemented with 0.1, 0.3, 0.5, and 0.7 mg/kg of Se from DL-SeMet, respectively. The trial lasted for 42 days. Results: Pigs supplemented with 0.3 and 0.7 mg/kg DL-SeMet obtained better feed gain ratio (P 〈 0.05). The best antioxidant ability (serum, liver, and muscle) was shown in 0.1-0.3 mg/kg DL-SeMet groups (P〈 0.05). The plasma Se concentration increased as the dietary DL-SeMet level elevated. The immunity among groups was not affected. Conclusions: DL-SeMet supplementation in the diet significantly improved the growth performance, antioxidant ability and plasma Se content of weaning pigs. DL-SeMet can replace sodium selenite in the diet of weaning pigs.
文摘[Objective] The paper was to determine whether the germplasm resources of Congjiang fragrance pig had changed over the past 30 years. [Method]The growth development and reproductive performance of 100 pigs from six towns in central fragrance pig producing area and Congjiang fragrance pig stock seed farm were measured,and further compared with the data in Guizhou Livestock and Poultry Breeds 1986. [Result]The growth and development status was basically consistent,and the average litter size was at a downward trend: the first litter decreased by 26. 2%; the second litter decreased by 7. 0%; the third litter decreased by8. 55%. [Conclusion] The study laid the foundation for vigorous promotion of Congjiang fragrance pig in industrial development process,acceleration of breeding pace,gradual updating of farming breeds by farmers and improvement of production efficiency of fragrance pig.
基金supported by the Key Program of Transgenic Plant Breeding,China (2008ZX08003-002)the Major Special Project of Guangdong Province,China(2009A080303009)+1 种基金the National Major Science Research Program of China (2009CB941601)the Special Fund for Agro-Scientific Research in the Public Interest,China (Agriculture,201003011)
文摘Two experiments were conducted to investigate the effect of dietary supplementation with phytase transgenic corn (PTC) on growth performance,phosphorus (P) utilization and excretion in growing pigs.In Exp.1,180 pigs (Large White × Landrace,BW=37.7 kg) were randomly allotted to 4 treatments with 5 replicates of 9 pigs each in order to evaluate the effect of PTC supplementation in low-P diets on growth performance.Four corn soybean meal-based diets consisted of a positive control (PC) diet,a diet containing 500 units (U) of exogenous phytase kg-1 (EP) on the basis of low-P (inorganic P reduced by 0.05% from PC diet) and the low-P+500 (PTC1) or 750 (PTC2) phytase U of PTC kg-1.In Exp.2,20 barrows (Large White×Landrace,BW=31 kg,4 treatments with 5 replicates of 1 pig each) were randomly selected to evaluate the effect of PTC in low-P diets on serum parameters and nutrient utilization.Diets in Exp.2 were similar to those in Exp.1 except that the EP group was replaced by a low-P diet without exogenous phytase supplementation as a negative control (NC) group.The results from Exp.1 showed that the average daily gain (ADG) in the PTC2 group was significantly higher (P〈0.05) than that in the EP group over all periods.On the other hand,the feed:gain (F:G) ratio of the EP group was significantly higher (P〈0.05) than that of the PTC2 group during 1-21 and 1-42 d,respectively.There were no differences in average daily feed intake (ADFI) among all treatments (P〉0.05).The results from Exp.2 showed that the concentration of serum Ca in the NC group was the highest (P〈0.05),while the concentration of serum P in the PTC2 group was the highest (P〈0.05) among all treatments.There was a significant decrease (P〈0.05) in the P apparent digestibility of the NC group compared with the other groups,and that of PTC2 group was the best.Furthermore,fecal P excretion was reduced (P〈0.05) from 1.80 g d-1 in the PC group to 1.28 g d-1 in the PTC2 group.In conclusion,dietary supplementation with PTC could reduce the application of inorganic P,decrease fecal P excretion,and improve the growth performance of growing pigs.
文摘Both quantitatively and qualitatively, underfeeding materialized by underconsumption of meat remains one of the things most felt in Africa. To make up for this deficit, emphasis is placed on the production of short-cycle animals such as poultry, rabbits and pork. Among these animals, pig plays a very important socio-economic role in the life of local populations. Its meat is required in various ceremonies such as the dowry and funerals. For this purpose, twenty four males cross piglets with Large White × Landrace × Duroc × Pietrain phenotypic traits, aged between 58 and 62 days and having an average weight of 9.978 ± 1.11 kg were used to evaluate the influence of graded levels of bovine rumen content on the growth performances of grower finisher pigs. The piglets were submitted to 4 treatments, housed in pairs and fed ad libitum with a diet containing 0, 5%, 10% and 20% bovine rumen content. The trial which lasted for 18 weeks was carried out at the Teaching and Research Farm of the University of Dschang. The results showed that drying process destroyed most microbes found in the rumen content. These results also showed that the incorporation of bovine rumen content in diets had no significant (p > 0.05) effect on feed conversion ratio efficiency. However, feed intake, body weight and body weight gain were significantly higher in pigs fed with a diet (R<sub>5</sub>) containing 5% of bovine rumen content. Feed cost per kg of live body weight was significantly (p <sub>20</sub> diet compared to the animals receiving the control diet which was lower than that of the other two diets (R<sub>0</sub>) and (R<sub>5</sub>) but comparable with animals fed diet R<sub>10</sub>. The analysis of the carcass showed that increasing incorporation level of bovine rumen content in the diet reduces abdominal fat and back fat thickness. For offal, the lowest weight was recorded in animals fed the control diet (R<sub>0</sub>) and the highest weight with animals fed a diet containing 5% bovine rumen content. Carcass length of animals fed the control diet (R<sub>0</sub>) was significantly greater compared with the carcass of animals fed diet R<sub>10</sub> and comparable to that of the other two treatments. The pH of the pork was comparable (p > 0.05) in all the treatments. In conclusion, a diet containing 5% bovine rumen content induced the best growth performances compared to all the other groups, while diet R<sub>20</sub> produced lean pork with the higher production cost.
基金funded by the National Nature Science Foundation(No.30000120).
文摘Ninety finishing pigs were selected to study the effect of N-methyl-aspartate and betaine on the internal growth hormone level in the serum and the correlation between the growth hormone level, growth performance and carcass characteristic of finishing pig. The study showed that the two matters could improve pig growth and carcass composition significantly. The correlation analyses indicated that the growth hormone and IGF-I have a positive correlation with the growth rate. Carcass lean ratio, longissimus dorsi area, serum free fatty acid and lipase activity have a negative correlation with the feed conversion ratio, carcass fat ratio and urine nitrogen. But the growth hormone is more effective than IGF-I (P<0.01). The results implicated that both the two matters may act through growth hormone axis (growth hormone - IGF-I) to manipulate pig growth.
基金Supported by Research Program of Science and Technology Department of Guizhou Province(QKH[2011]5020)
文摘Ten healthy weaning piglets of Landrace x Yorkshire (LY) in good body condition with body weight of (24 ± 1.27) kg were selected, and ten weaning piglets of Berkshire × LY (BLY) and Duroc × LY (DLY) with similar situation were also selected respectively to study the growth performance of different cross combinations. Three groups of experimental pigs were bred with conventional diet. The results showed that the average dry matter intakes of combinations of BLY and DLY were slightly decreased by 4.97% and 5.47% compared with the combination of LY, while average daily gain was significantly increased by 3.13% and 1.14%, and feed-weight ratio was decreased by 7.94% and 6.35%, respectively; economic benefit per pig was increased by 0.61 and 0.41 yuan, respectively. The combinations of BLY and DLY have better growth performance, which can be promoted in the production.