Growth performance as well as marbling is the main breeding objectives in Japanese Black cattle, the major beef breed in Japan. A previous study has identified the c.1326T>G single nucleotide polymorphism (SNP) in ...Growth performance as well as marbling is the main breeding objectives in Japanese Black cattle, the major beef breed in Japan. A previous study has identified the c.1326T>G single nucleotide polymorphism (SNP) in the NCAPG (non-SMC condensin I complex, subunit G) gene that leads to the amino acid change p.Ile442Met in the NCAPG protein, which is a candidate causative variation for a bovine carcass weight (CWT) quantitative trait locus (QTL). In this study, we first confirmed the association of the c.1326T>G SNP with the growth-related traits, CWT, rib eye area (REA) and rib thickness (RT), and showed significant effect of the SNP genotypes on the marbling trait, beef marbling score (BMS), in the Japanese Black beef cattle population of the Niigata prefecture, with the G allele being associated with a favorable phenotype of these traits. Thus, we concluded that the c.1326T>G SNP is useful for effective marker-assisted selection to increase meat quality and meat productivity in Japanese Black beef cattle of the Niigata prefecture. Furthermore the frequency of the favorable G allele of the c.1326T>G SNP in the Niigata prefecture population was significantly lower than the frequency of this allele in Japanese Black cattle population of the Kagoshima prefecture. However, no statistically significant difference was detected between the allele frequencies estimated by maternal alleles in the half-sib progeny steers in the Niigata prefecture population and obtained in Japanese Black cattle population of the Kagoshima prefecture.展开更多
Recent studies indicated that interleukin(IL)-17, growth-related oncogene(GRO)-α and IL-8 play an important role in the pathogenesis of nasal polyps. However, the effects of the increased amount of IL-17 and the ...Recent studies indicated that interleukin(IL)-17, growth-related oncogene(GRO)-α and IL-8 play an important role in the pathogenesis of nasal polyps. However, the effects of the increased amount of IL-17 and the production of GRO-α and IL-8 in human nasal polyp fibroblasts are not completely understood. This study aimed to determine the effects of the increased IL-17 on the changes of GRO-α and IL-8 expression in human nasal polyp fibroblasts and further investigate the mechanism of neutrophil infiltration in nasal polyps. Nasal polyp fibroblasts were isolated from six cases of human nasal polyps, and the cells were stimulated with five different concentrations of IL-17. Real-time fluorescence quantitative polymerase chain reaction(RT-PCR) was used to detect the mRNA expression of GRO-α and IL-8. The mRNA of GRO-α and IL-8 was expressed in unstimulated controls and remarkably increased by stimulation with IL-17. Moreover, the levels of GRO-α and IL-8 produced by fibroblasts were increased gradually with the increases in IL-17 concentrations. The present study showed that nasal fibroblasts can produce GRO-α and IL-8, and their production is remarkably enhanced by IL-17 stimulation, thereby clarifying the mechanism of the IL-17 mediated neutrophil infiltration in nasal polyps. These findings might provide a rationale for using IL-17 inhibitors as a treatment for nasal inflammatory diseases such as nasal polyps.展开更多
The common carp(Cyrpinus carpio L.)is the oldest cultured and the most domesticated fish species,as well as one of the most important freshwater fishes in the world.However,scientific studies on evaluating the growth-...The common carp(Cyrpinus carpio L.)is the oldest cultured and the most domesticated fish species,as well as one of the most important freshwater fishes in the world.However,scientific studies on evaluating the growth-related quantitative traits in this fish are limited.Heritability,the most important parameter in selective breeding programs,was extensively studied for the growth-related traits.The values varied widely among the experiments and methods used because of the existence of common environmental,dominance and maternal effects.However,correlations in phenotypic and genetic levels first evaluated several years ago were limited.On the other hand,heterosis was widely reported and easily obtained for growth-related traits in the common carp.Meanwhile,genotype environment interaction and prediction of breeding values have been studied recently,and are very important in conducting selective breeding programs.The developmental quantitative genetics of growth-related traits was first analyzed in the common carp for reasonable selection during ontogeny.It is expected that genetic improvement will be achieved by carrying out direct selective breeding in the common carp.展开更多
Genetic breeding is an important approach to improve the economic traits of aquaculture animals.Unfortunately,the genetic basis for important economic traits of red swamp crayfish,including growth-related traits,has n...Genetic breeding is an important approach to improve the economic traits of aquaculture animals.Unfortunately,the genetic basis for important economic traits of red swamp crayfish,including growth-related traits,has not yet been reported.In the present study,two full-sib families and two natural populations were employed to identify quantitative trait loci(QTLs)for growth-related traits of red swamp crayfish.In total,28 QTLs for five growth-related traits,including body weight,body length,carapace length,carapace width,and abdomen length,were repeatedly identified in more than two families/populations,by means of association analysis;nine of these displayed a heterosis effect.The phenotypic variations of the five investigated traits explained by the 28 QTLs ranged from 4.2%to 19.0%.Eight additive and three heterosis QTLs were further successfully validated using comparative analysis of favoured alleles between the small and big size crayfish groups in the natural population.In conclusion,this study investigated the genetic basis of growth-related traits in red swamp crayfish,which is of great significance for their application in the future molecular breeding of the species.展开更多
Iso-nitrogenous and iso-lipidic diets containing 0%,3%,6%,9%,and 12%hydrolyzed porcine mucosa(namely,HPM0,HPM3,HPM6,HPM9,and HPM12)were prepared to evaluate their effects on the growth performance,muscle nutrition com...Iso-nitrogenous and iso-lipidic diets containing 0%,3%,6%,9%,and 12%hydrolyzed porcine mucosa(namely,HPM0,HPM3,HPM6,HPM9,and HPM12)were prepared to evaluate their effects on the growth performance,muscle nutrition composition,texture property,and gene expression related to muscle growth of hybrid groupers(Epinephelus fuscoguttatus\Epinephelus lanceolatus_).Groupers were fed to apparent satiation at 08:00 and 16:00 every day for a total of 56 days.It was found that the weight gain percentage in the HPM0,HPM3,and HPM6 groups did not differ(P>0.05).The cooking loss and drip loss of the dorsal muscle in the HPM3 group were lower than those in the HPM6 and HPM9 groups(P<0.05).The hardness and chewiness of the dorsal muscle in the HPM3 group were higher than those in the HPM0,HPM9,and HPM12 groups(P<0.05).The gumminess in the HPM3 group was higher than that in the HPM9 and HPM12 groups(P<0.05).The total essential amino acid content of the dorsal muscle in the HPM12 group was higher than that in the HPM0 group(P<0.05).The contents of total n-3 polyunsaturated fatty acid and total n-3 highly unsaturated fatty acid,as well as the ratio of n-3/n-6 polyunsaturated fatty acid in the dorsal muscle was higher in the HPM0 group than in all other groups(P<0.05).The relative expressions of gene myogenic factor 5,myocyte enhancer factor 2c,myocyte enhancer factor 2a,myosin heavy chain,transforming growth factor-beta 1(TGF-b1),and follistatin(FST)were the highest in the dorsal muscle of the HPM3 group.The results indicated that the growth performance of hybrid grouper fed a diet with 6%HPM and 27%fish meal was as good as that of the HPM0 group.When fish ingested a diet containing 3%HPM,the expression of genes TGF-β1 and FST involved in muscle growth were upregulated,and then the muscle quality related to hardness and chewiness were improved.An appropriate amount of HPM could be better used in grouper feed.展开更多
文摘Growth performance as well as marbling is the main breeding objectives in Japanese Black cattle, the major beef breed in Japan. A previous study has identified the c.1326T>G single nucleotide polymorphism (SNP) in the NCAPG (non-SMC condensin I complex, subunit G) gene that leads to the amino acid change p.Ile442Met in the NCAPG protein, which is a candidate causative variation for a bovine carcass weight (CWT) quantitative trait locus (QTL). In this study, we first confirmed the association of the c.1326T>G SNP with the growth-related traits, CWT, rib eye area (REA) and rib thickness (RT), and showed significant effect of the SNP genotypes on the marbling trait, beef marbling score (BMS), in the Japanese Black beef cattle population of the Niigata prefecture, with the G allele being associated with a favorable phenotype of these traits. Thus, we concluded that the c.1326T>G SNP is useful for effective marker-assisted selection to increase meat quality and meat productivity in Japanese Black beef cattle of the Niigata prefecture. Furthermore the frequency of the favorable G allele of the c.1326T>G SNP in the Niigata prefecture population was significantly lower than the frequency of this allele in Japanese Black cattle population of the Kagoshima prefecture. However, no statistically significant difference was detected between the allele frequencies estimated by maternal alleles in the half-sib progeny steers in the Niigata prefecture population and obtained in Japanese Black cattle population of the Kagoshima prefecture.
基金supported by grants from Research and National Promotion of Early Detection,Standardized Diagnosisand Treatment,and Preventive Strategy for Major Otology and Rhinologic Diseases(No.201202005)Wu Jieping Medical Foundation(No.LC1345)Foundation of Hubei ProvinceKey Laboratory of Molecular Imaging(No.02.03.2013-64)
文摘Recent studies indicated that interleukin(IL)-17, growth-related oncogene(GRO)-α and IL-8 play an important role in the pathogenesis of nasal polyps. However, the effects of the increased amount of IL-17 and the production of GRO-α and IL-8 in human nasal polyp fibroblasts are not completely understood. This study aimed to determine the effects of the increased IL-17 on the changes of GRO-α and IL-8 expression in human nasal polyp fibroblasts and further investigate the mechanism of neutrophil infiltration in nasal polyps. Nasal polyp fibroblasts were isolated from six cases of human nasal polyps, and the cells were stimulated with five different concentrations of IL-17. Real-time fluorescence quantitative polymerase chain reaction(RT-PCR) was used to detect the mRNA expression of GRO-α and IL-8. The mRNA of GRO-α and IL-8 was expressed in unstimulated controls and remarkably increased by stimulation with IL-17. Moreover, the levels of GRO-α and IL-8 produced by fibroblasts were increased gradually with the increases in IL-17 concentrations. The present study showed that nasal fibroblasts can produce GRO-α and IL-8, and their production is remarkably enhanced by IL-17 stimulation, thereby clarifying the mechanism of the IL-17 mediated neutrophil infiltration in nasal polyps. These findings might provide a rationale for using IL-17 inhibitors as a treatment for nasal inflammatory diseases such as nasal polyps.
基金supported by the Natural Science Foundation of China(Grant No.30700622)the Shanghai Education Committee Project(No.07ZZ136)the Shanghai Leading Academic Discipline Project(No.Y1101).
文摘The common carp(Cyrpinus carpio L.)is the oldest cultured and the most domesticated fish species,as well as one of the most important freshwater fishes in the world.However,scientific studies on evaluating the growth-related quantitative traits in this fish are limited.Heritability,the most important parameter in selective breeding programs,was extensively studied for the growth-related traits.The values varied widely among the experiments and methods used because of the existence of common environmental,dominance and maternal effects.However,correlations in phenotypic and genetic levels first evaluated several years ago were limited.On the other hand,heterosis was widely reported and easily obtained for growth-related traits in the common carp.Meanwhile,genotype environment interaction and prediction of breeding values have been studied recently,and are very important in conducting selective breeding programs.The developmental quantitative genetics of growth-related traits was first analyzed in the common carp for reasonable selection during ontogeny.It is expected that genetic improvement will be achieved by carrying out direct selective breeding in the common carp.
基金the key research and development program of Hubei province(2021BBA232)the earmarked fund for CARS-48,and the research start-up fund of high level talent introduction of Huazhong Agricultural University.
文摘Genetic breeding is an important approach to improve the economic traits of aquaculture animals.Unfortunately,the genetic basis for important economic traits of red swamp crayfish,including growth-related traits,has not yet been reported.In the present study,two full-sib families and two natural populations were employed to identify quantitative trait loci(QTLs)for growth-related traits of red swamp crayfish.In total,28 QTLs for five growth-related traits,including body weight,body length,carapace length,carapace width,and abdomen length,were repeatedly identified in more than two families/populations,by means of association analysis;nine of these displayed a heterosis effect.The phenotypic variations of the five investigated traits explained by the 28 QTLs ranged from 4.2%to 19.0%.Eight additive and three heterosis QTLs were further successfully validated using comparative analysis of favoured alleles between the small and big size crayfish groups in the natural population.In conclusion,this study investigated the genetic basis of growth-related traits in red swamp crayfish,which is of great significance for their application in the future molecular breeding of the species.
基金This study was supported by the Fund of The National Key R&D Program of China(2019YFD0900200)Southern Marine Science and Engineering Guangdong Laboratory(Zhanjiang)(No.ZJW2019-06)+1 种基金China Agricultural Research System(CARS-47)Guangdong Modern Agriculture Research System-Innovation Team(2020KJ150).
文摘Iso-nitrogenous and iso-lipidic diets containing 0%,3%,6%,9%,and 12%hydrolyzed porcine mucosa(namely,HPM0,HPM3,HPM6,HPM9,and HPM12)were prepared to evaluate their effects on the growth performance,muscle nutrition composition,texture property,and gene expression related to muscle growth of hybrid groupers(Epinephelus fuscoguttatus\Epinephelus lanceolatus_).Groupers were fed to apparent satiation at 08:00 and 16:00 every day for a total of 56 days.It was found that the weight gain percentage in the HPM0,HPM3,and HPM6 groups did not differ(P>0.05).The cooking loss and drip loss of the dorsal muscle in the HPM3 group were lower than those in the HPM6 and HPM9 groups(P<0.05).The hardness and chewiness of the dorsal muscle in the HPM3 group were higher than those in the HPM0,HPM9,and HPM12 groups(P<0.05).The gumminess in the HPM3 group was higher than that in the HPM9 and HPM12 groups(P<0.05).The total essential amino acid content of the dorsal muscle in the HPM12 group was higher than that in the HPM0 group(P<0.05).The contents of total n-3 polyunsaturated fatty acid and total n-3 highly unsaturated fatty acid,as well as the ratio of n-3/n-6 polyunsaturated fatty acid in the dorsal muscle was higher in the HPM0 group than in all other groups(P<0.05).The relative expressions of gene myogenic factor 5,myocyte enhancer factor 2c,myocyte enhancer factor 2a,myosin heavy chain,transforming growth factor-beta 1(TGF-b1),and follistatin(FST)were the highest in the dorsal muscle of the HPM3 group.The results indicated that the growth performance of hybrid grouper fed a diet with 6%HPM and 27%fish meal was as good as that of the HPM0 group.When fish ingested a diet containing 3%HPM,the expression of genes TGF-β1 and FST involved in muscle growth were upregulated,and then the muscle quality related to hardness and chewiness were improved.An appropriate amount of HPM could be better used in grouper feed.