Zur Aufklarung der Rolle dreier DNA Reparaturenzyme in der Hepatokarzino genese haben wir die hMTH 1,hOGG 1 und hMYHα mRNA Expression mittels RT/semi quantitativer Echtzeit PCR und der 8 OHdG Gehalt mittels H...Zur Aufklarung der Rolle dreier DNA Reparaturenzyme in der Hepatokarzino genese haben wir die hMTH 1,hOGG 1 und hMYHα mRNA Expression mittels RT/semi quantitativer Echtzeit PCR und der 8 OHdG Gehalt mittels HPLC/ECD in Tumor und Peritumorgewebe von 21 Patienten mit hepatozellularem Karzinom (HCC ) bestimmt. Es wurde gezeigt, da der 8 OHdG Gehalt im Peritumorgewebe signif ikant hoher als im Tumorgeweb e war ( P =0.006) und mit dem Entzundungsgrad korrelierte. Die hMTH 1 E xpression war im Tumorgewebe gegenuber dem Peritumorgewebe erhoht ( P =0.014). Umgekehrt war die hMYHα Expression im Peritumorgewebe signifikant hoher als im Tumorgewebe ( P =0.039). Fur die hOGG 1 Expression wurde kein deutlicher Unterschied zwischen Tumor und Peritumorgewebe beobachte t. Eine deutliche lineare Korrelation zwischen der hMTH 1 und der hOGG 1 Exp ression wurde sowohl in Tumor ( r =0.809, P < 0.001) als auch in Peritum orgewebe ( r =0.883, P <0.001) gefunden. Diese Daten sprechen fur ein e reaktive und gegen eine pathogenetische Rolle der untersuchten DNA Reparature nzyme in der Hepatokarzinogenese.展开更多
To observe the alteration in the expression of DNA repair enzymes hOGG1 and hMYHa and the change in 8-OHdG levels in the HBx gene-transfected cells HepG2/HBx and to explore the mechanisms of the HBV-associated hepatoc...To observe the alteration in the expression of DNA repair enzymes hOGG1 and hMYHa and the change in 8-OHdG levels in the HBx gene-transfected cells HepG2/HBx and to explore the mechanisms of the HBV-associated hepatocellular carcinoma, the gene-transfected cells HepG2/HBx which stably expressed HBx was established, and the effect of HBx on the cell cycle and proliferation of HepG2 was examined. By using the β-actin as the interior control, real-time polymerase chain reaction （Real-time qPCR） was employed to quantitatively detect the expression of DNA repair enzymes hOGG1 and hMYHα in the HepG2/HBx, the control cells HepG2 and HepG2 transfected with pcDNA3.1 vector （HepG2/pDNA3.1）. The 8-OHdG levels were determined by HPLC/ECD in the established gene-transfected cells HepG2/HBx and the control cells HepG2 and HepG2/pcDNA3.1. Our results showed that the expression of DNA repair enzyme hMYHα in the HepG2/HBx （0.021±0.007） was significantly lower than that of HepG2 （0.099±0.041） （P〈0.05） and HepG2/pDNA3.1 （0.121±0.005） （P〈0.05）. However, the no significant differences existed in the expression of DNA repair enzyme hOGG1 among the three cell strains （P〉0.05）. The 8-OHdG level in the HepG2/HBx was significantly higher than that in HepG2 and HepG2/pcDNA3.1 （P〈0.05）. It is concluded that HBx gene may inhibit the expression of DNA repair enzyme hMYHα mRNA to impair the ability to repair the intracellular DNA oxidative damage, to increase the oxidative DNA-adduct 8-OHdG and to affect the nucleotide excision repair function, thus participate in the occurrence and development of hepatocellular carcinoma.展开更多
Two new genera of the ant subfamilies Dorylinae and Ponerinae are collected and described from the tropical rain forest of Xishuangbanna Nature Reserve,Yunnan Province,China. Yunodorylus gen nov belongs to the subf...Two new genera of the ant subfamilies Dorylinae and Ponerinae are collected and described from the tropical rain forest of Xishuangbanna Nature Reserve,Yunnan Province,China. Yunodorylus gen nov belongs to the subfamily Dorylinae,which is monotypic and terrestrial with 2 colonies of the species Y sexspinus sp nov. collected from the soil sample of seasonal rain forest and deciduous monsoon forest. Bannapone gen nov. belongs to the tribe Amblyoponini of Ponerinae,which is also monotypic and known only from a dealate female of the species B mulanae sp nov.collected from a soil sample in semi evergreen monsoon forest.展开更多
文摘Zur Aufklarung der Rolle dreier DNA Reparaturenzyme in der Hepatokarzino genese haben wir die hMTH 1,hOGG 1 und hMYHα mRNA Expression mittels RT/semi quantitativer Echtzeit PCR und der 8 OHdG Gehalt mittels HPLC/ECD in Tumor und Peritumorgewebe von 21 Patienten mit hepatozellularem Karzinom (HCC ) bestimmt. Es wurde gezeigt, da der 8 OHdG Gehalt im Peritumorgewebe signif ikant hoher als im Tumorgeweb e war ( P =0.006) und mit dem Entzundungsgrad korrelierte. Die hMTH 1 E xpression war im Tumorgewebe gegenuber dem Peritumorgewebe erhoht ( P =0.014). Umgekehrt war die hMYHα Expression im Peritumorgewebe signifikant hoher als im Tumorgewebe ( P =0.039). Fur die hOGG 1 Expression wurde kein deutlicher Unterschied zwischen Tumor und Peritumorgewebe beobachte t. Eine deutliche lineare Korrelation zwischen der hMTH 1 und der hOGG 1 Exp ression wurde sowohl in Tumor ( r =0.809, P < 0.001) als auch in Peritum orgewebe ( r =0.883, P <0.001) gefunden. Diese Daten sprechen fur ein e reaktive und gegen eine pathogenetische Rolle der untersuchten DNA Reparature nzyme in der Hepatokarzinogenese.
基金supported by a grant from the National Natural Sciences Foundation of China (No.30570821)
文摘To observe the alteration in the expression of DNA repair enzymes hOGG1 and hMYHa and the change in 8-OHdG levels in the HBx gene-transfected cells HepG2/HBx and to explore the mechanisms of the HBV-associated hepatocellular carcinoma, the gene-transfected cells HepG2/HBx which stably expressed HBx was established, and the effect of HBx on the cell cycle and proliferation of HepG2 was examined. By using the β-actin as the interior control, real-time polymerase chain reaction （Real-time qPCR） was employed to quantitatively detect the expression of DNA repair enzymes hOGG1 and hMYHα in the HepG2/HBx, the control cells HepG2 and HepG2 transfected with pcDNA3.1 vector （HepG2/pDNA3.1）. The 8-OHdG levels were determined by HPLC/ECD in the established gene-transfected cells HepG2/HBx and the control cells HepG2 and HepG2/pcDNA3.1. Our results showed that the expression of DNA repair enzyme hMYHα in the HepG2/HBx （0.021±0.007） was significantly lower than that of HepG2 （0.099±0.041） （P〈0.05） and HepG2/pDNA3.1 （0.121±0.005） （P〈0.05）. However, the no significant differences existed in the expression of DNA repair enzyme hOGG1 among the three cell strains （P〉0.05）. The 8-OHdG level in the HepG2/HBx was significantly higher than that in HepG2 and HepG2/pcDNA3.1 （P〈0.05）. It is concluded that HBx gene may inhibit the expression of DNA repair enzyme hMYHα mRNA to impair the ability to repair the intracellular DNA oxidative damage, to increase the oxidative DNA-adduct 8-OHdG and to affect the nucleotide excision repair function, thus participate in the occurrence and development of hepatocellular carcinoma.
文摘Two new genera of the ant subfamilies Dorylinae and Ponerinae are collected and described from the tropical rain forest of Xishuangbanna Nature Reserve,Yunnan Province,China. Yunodorylus gen nov belongs to the subfamily Dorylinae,which is monotypic and terrestrial with 2 colonies of the species Y sexspinus sp nov. collected from the soil sample of seasonal rain forest and deciduous monsoon forest. Bannapone gen nov. belongs to the tribe Amblyoponini of Ponerinae,which is also monotypic and known only from a dealate female of the species B mulanae sp nov.collected from a soil sample in semi evergreen monsoon forest.
