The establishment of nucleus herds (NHs) of Native Pigs (NPs) at various R&D stations in the Philippines is currently being undertaken for food security and genetic conservation advocacy. Marker-assisted selection...The establishment of nucleus herds (NHs) of Native Pigs (NPs) at various R&D stations in the Philippines is currently being undertaken for food security and genetic conservation advocacy. Marker-assisted selection (MAS) is being utilized to identify individuals carrying favorable alleles of genes associated with production traits and screen out genetic defects (GD) for breeding purposes. Porcine Stress Syndrome (PSS) caused by a mutation in Halothane (HAL) gene is a GD frequently found in commercial breeds that when expressed, causes pale, soft, exudative (PSE) meat. PSE is inferior quality meat undesirable in the market causing economic losses to the swine industry. Thus, this study was conducted to screen the HAL gene through mutagenically separated-polymerase chain reaction (MSPCR) in selected NP herds and assessed its repeatability in local breeds. Results showed that out of 577 screened individuals, 543 (94.11%) were normal (NN), 0 (0%) were homozygous mutant (nn) and 34 (5.89%) were heterozygous carriers (Nn). Therefore, the optimized PSS screening protocol using MSPCR is also applicable to local breeds. As such, the availability of genetic tests for PSS could be useful in improving the Philippine NPs breeding selection and inhibiting or eliminating PSS mutant incidence within its nucleus herd.展开更多
[Objective] The aim was to investigate the distribution of the halothane （Haln） gene in breeding pig group in Beijing area.[Method] PCR-RFLP method was used to determine Haln genotype of 609 breeding pigs from boar ...[Objective] The aim was to investigate the distribution of the halothane （Haln） gene in breeding pig group in Beijing area.[Method] PCR-RFLP method was used to determine Haln genotype of 609 breeding pigs from boar stations and pig breeding farms in Beijing.[Result] The Haln gene frequencies of Large White,Landrace,Duroc and Pietrain pigs of the boar stations were 0,17.11%,5.56% and 18.75%,respectively; and the Haln gene frequencies of Large White,Landrace and Duroc pigs of pig breeding farms were 4.52%,11.68% and 23.33%,respectively.[Conclusion] The result provided a basis for the rational use of Haln gene.展开更多
A simple preparation using the ear tissue for PCR amplification was established for diagnosis of genotypes for halothane in 181 sows.3 halothane heterozygous pigs were detected.The behaviors of the sows that have diff...A simple preparation using the ear tissue for PCR amplification was established for diagnosis of genotypes for halothane in 181 sows.3 halothane heterozygous pigs were detected.The behaviors of the sows that have different genotypes were observed.The heterozygous sows expressed seem more behavioral stereotypies than halothane resistant.But there is no difference in two genotypes.The behaviour directed trough in heterozygous sows is higher than halothane resistant.展开更多
为了明确肉质相关基因氟烷基因(Hal)、酸肉基因(RN)和脂肪肥胖相关基因(FTO)在国内外不同猪种群体间的遗传变异特性,本研究以大约克、长白、杜洛克和金华猪等猪种群体为研究对象,应用PCR-RFLP技术分别检测了Hal、RN和FTO基因的多态性,...为了明确肉质相关基因氟烷基因(Hal)、酸肉基因(RN)和脂肪肥胖相关基因(FTO)在国内外不同猪种群体间的遗传变异特性,本研究以大约克、长白、杜洛克和金华猪等猪种群体为研究对象,应用PCR-RFLP技术分别检测了Hal、RN和FTO基因的多态性,并分析了FTO基因上2个多态位点g.276G>T和c.594C>G的遗传变异情况。结果表明:(1)在杜洛克猪中发现了Hal的基因型Hal NHal n,其频率为0.166,但未发现基因型Hal n Hal n,其它猪种群体中仅检测到了基因型Hal NHal N;(2)在已检测的所有猪种群体中只检测到RN基因的rn/rn基因型,未发现rn/RN和RN/RN基因型;(3)在FTO的g.276G>T位点上,金华猪呈现单态,其它猪种群体均呈现多态。在FTO的c.594C>G位点上,4个猪种群体均呈现多态,3个外来猪种群体均以CC基因型频率较高,而金华猪则以GG基因型频率较高。研究结果提示,由于Hal基因在养猪生产中的利弊双重性,因此在某些猪种群体中仍然存在较高频率的Hal NHal n基因型。此外,结合FTO基因的生理功能和已有的研究结果,可在特定的猪群中将其作为影响猪肉质性状的候选基因。本研究发现FTO的基因型分布在我国优良地方猪种金华猪与国外3个种猪群间存在较大差异,为深入研究不同品种猪的肉质形成机理提供了基础数据。展开更多
文摘The establishment of nucleus herds (NHs) of Native Pigs (NPs) at various R&D stations in the Philippines is currently being undertaken for food security and genetic conservation advocacy. Marker-assisted selection (MAS) is being utilized to identify individuals carrying favorable alleles of genes associated with production traits and screen out genetic defects (GD) for breeding purposes. Porcine Stress Syndrome (PSS) caused by a mutation in Halothane (HAL) gene is a GD frequently found in commercial breeds that when expressed, causes pale, soft, exudative (PSE) meat. PSE is inferior quality meat undesirable in the market causing economic losses to the swine industry. Thus, this study was conducted to screen the HAL gene through mutagenically separated-polymerase chain reaction (MSPCR) in selected NP herds and assessed its repeatability in local breeds. Results showed that out of 577 screened individuals, 543 (94.11%) were normal (NN), 0 (0%) were homozygous mutant (nn) and 34 (5.89%) were heterozygous carriers (Nn). Therefore, the optimized PSS screening protocol using MSPCR is also applicable to local breeds. As such, the availability of genetic tests for PSS could be useful in improving the Philippine NPs breeding selection and inhibiting or eliminating PSS mutant incidence within its nucleus herd.
基金Supported by Excellent Talents Training Funded Projects of Organization Department of CPC Beijing Municipal Committee(20071D0200100039)Swine Industry Technology~~
文摘[Objective] The aim was to investigate the distribution of the halothane （Haln） gene in breeding pig group in Beijing area.[Method] PCR-RFLP method was used to determine Haln genotype of 609 breeding pigs from boar stations and pig breeding farms in Beijing.[Result] The Haln gene frequencies of Large White,Landrace,Duroc and Pietrain pigs of the boar stations were 0,17.11%,5.56% and 18.75%,respectively; and the Haln gene frequencies of Large White,Landrace and Duroc pigs of pig breeding farms were 4.52%,11.68% and 23.33%,respectively.[Conclusion] The result provided a basis for the rational use of Haln gene.
文摘A simple preparation using the ear tissue for PCR amplification was established for diagnosis of genotypes for halothane in 181 sows.3 halothane heterozygous pigs were detected.The behaviors of the sows that have different genotypes were observed.The heterozygous sows expressed seem more behavioral stereotypies than halothane resistant.But there is no difference in two genotypes.The behaviour directed trough in heterozygous sows is higher than halothane resistant.
文摘为了明确肉质相关基因氟烷基因(Hal)、酸肉基因(RN)和脂肪肥胖相关基因(FTO)在国内外不同猪种群体间的遗传变异特性,本研究以大约克、长白、杜洛克和金华猪等猪种群体为研究对象,应用PCR-RFLP技术分别检测了Hal、RN和FTO基因的多态性,并分析了FTO基因上2个多态位点g.276G>T和c.594C>G的遗传变异情况。结果表明:(1)在杜洛克猪中发现了Hal的基因型Hal NHal n,其频率为0.166,但未发现基因型Hal n Hal n,其它猪种群体中仅检测到了基因型Hal NHal N;(2)在已检测的所有猪种群体中只检测到RN基因的rn/rn基因型,未发现rn/RN和RN/RN基因型;(3)在FTO的g.276G>T位点上,金华猪呈现单态,其它猪种群体均呈现多态。在FTO的c.594C>G位点上,4个猪种群体均呈现多态,3个外来猪种群体均以CC基因型频率较高,而金华猪则以GG基因型频率较高。研究结果提示,由于Hal基因在养猪生产中的利弊双重性,因此在某些猪种群体中仍然存在较高频率的Hal NHal n基因型。此外,结合FTO基因的生理功能和已有的研究结果,可在特定的猪群中将其作为影响猪肉质性状的候选基因。本研究发现FTO的基因型分布在我国优良地方猪种金华猪与国外3个种猪群间存在较大差异,为深入研究不同品种猪的肉质形成机理提供了基础数据。
