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Salt Tolerance Mechanisms and Approaches:Future Scope of Halotolerant Genes and Rice Landraces 被引量:7
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作者 Tarun Bhatt Aditi Sharma +1 位作者 Sanjeev Puri Anu Priya Minhas 《Rice science》 SCIE CSCD 2020年第5期368-383,共16页
All rice plant developmental stages are severely affected by soil salinity. Salinity-induced ionic and osmotic stresses affect stomata closure and gaseous exchange, and reduce transpiration and the rate of carbon assi... All rice plant developmental stages are severely affected by soil salinity. Salinity-induced ionic and osmotic stresses affect stomata closure and gaseous exchange, and reduce transpiration and the rate of carbon assimilation, and hence decrease plant yield. Understanding the response of rice plants toward salinity stress at the genetic level and developing salt-tolerant varieties are the vital mandates for its effective management. This review described the present status of salt-tolerance achieved in rice by various mechanisms including the ion homeostasis(Na^+/H^+, OsNHX antiporters), compatible organic solutes(glycine betaine and proline), antioxidative genes(OsECS, OsVTE1, OsAPX and OsMSRA4.1), salt responsive regulatory elements(transcription factors, cis-acting elements and miRNAs) and genes ecoding protein kinases(MAPKs, SAPKs and STRKs). Further, the future perspective of developing salt-tolerant varieties lies in exploring halotolerant gene homologs from rice varieties, especially the landraces. Genetic diversity among rice landraces can serve as a valuable resource for future studies toward variety improvement through breeding and genome editing. Further, identification, multiplication, preservation and utilization of biodiversity among landraces are the urgent buffers to be saved as a heritage for future generations to come. 展开更多
关键词 RICE soil salinity ion homeostasis halotolerant LANDRACE
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Halomonas Erhaiensis sp. nov.,a Moderately Halotolerant Bacterium Isolated from Erhai Lake,Qinghai Province,China
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作者 ZHANG Guojing JIANG Hongchen +2 位作者 YANG Jian WU Geng LIU Wen 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2014年第S1期120-120,共1页
The strain CUG00003T was isolated from the sediment of Erhai Lake of Qing Hai Province,China.Results of 16S r RNA gene sequence analyses indicated a close relationship(97%similarity)of the isolate to
关键词 rod-shaped moderately halotolerant FLAVOBACTERIUM
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Halomonas Xiaochaidanensis sp. nov.,a Halotolerant Bacterium Isolated from Xiaochaidan Lake of Qadam Basin,China
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作者 LIU Wen JIANG Hongchen +2 位作者 YANG Jian WU Geng ZHANG Guojing 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2014年第S1期89-90,共2页
A short-rod-shaped halotolerant bacterium,designated CUG00002T,was isolated from the sediment of Xiaochaidan Lake of Qadam Basin,China,using R2A medium.The cells are Gram-negative,aerobic and forming creamy and circul... A short-rod-shaped halotolerant bacterium,designated CUG00002T,was isolated from the sediment of Xiaochaidan Lake of Qadam Basin,China,using R2A medium.The cells are Gram-negative,aerobic and forming creamy and circular colonies with diameter of 1- 展开更多
关键词 Halomonas xiaochaidanensis sp.nov. GRAM-NEGATIVE halotolerant
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A Novel Halotolerant Bacterium Isolated from El Golea Lake in Algeria and Antimicrobial Potential of this Strain
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作者 Nihel Klouche Khelil Nadia Aissaoui +2 位作者 Fatima Nas Jean Luc Cayol Lotfi Ghellai 《Journal of Agricultural Science and Technology(A)》 2013年第10期825-834,共10页
A halotolerant, gram positive, motile and rod, was isolated from the E1 Gol6a lake of Ghardaia, Alegria and screened for its antimicrobial potential. The strain showed an inhibitory effect to gram positive and gram ne... A halotolerant, gram positive, motile and rod, was isolated from the E1 Gol6a lake of Ghardaia, Alegria and screened for its antimicrobial potential. The strain showed an inhibitory effect to gram positive and gram negative bacteria against Cladosporium spp. and a slight inhibitory effect against C. albicans using chloroform as the extraction solvent and the nutrient broth as production medium. 16S rRNA sequencing showed that the isolate named LMB3981 is a new taxon in the family of Bacillaceae (with 96% similarity). The strain is close to Filobacillus milosensis and Bacillus haloalkaliphilum with 95% similarity. The phenotypic study showed differences between LMB3981 and two strains that are near and have confirmed the results of 16S rRNA sequencing by specific metabolic properties of the strain. 展开更多
关键词 New taxa EXTREMOPHILE antimicrobial molecules halotolerant Bacillus genus El Golea Lake.
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Study on halotolerance of phyllosoma larva of Chinese spiny lobster (Panulirus stimpsoni Holthuis)
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作者 Chen Changsheng, Chen Zhengqiang, Hu Jiacai, Ye Zhaohong, Ji Dehua, Lan Liangchun 1. Institute of Aquaculture Biotechnogy, Jimei University, Xiamen 361021, China 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2001年第3期409-415,共7页
The halotolerance of phyllosoma larva of Chinese spiny lobster (Panulirus stimpsoni Holthuis) was experimentally studied in a man-made seawater of 13, 15, 19, 23, 27, 31, 35, 39, 43, 47 and 49 salinity. The experiment... The halotolerance of phyllosoma larva of Chinese spiny lobster (Panulirus stimpsoni Holthuis) was experimentally studied in a man-made seawater of 13, 15, 19, 23, 27, 31, 35, 39, 43, 47 and 49 salinity. The experimental results showed that phyllosoma larve survived for 4~6 d with a rate of 25.0% to 47.5% if seawater salinity was increased or decreased gradually from 31 to 49 or to 13 respectively. In a range of 23 to 39 salinity, the phyllosoma larva grew, developed and metamorphosed normally, the metamorphosis rate of which in Stages II and III being 85% and 48% , respectively, when cultured in seawater of 31 salinity. It seems that some regular dispersion of larvae appeared in various salinities of seawater; In lower salinity, they mainly dispersed in the lower layer and in higher salinity,almost stayed in the upper and middle layers. Also, the results indicated that the phyllosoma larva of Chinese spiny lobster had a strong halotolerance to various salinities of seawater ranging from 15 to 47,in which 27 to 35 salinities were appropriate for larva growth and development. 展开更多
关键词 Panulirus stimpsoni Holthuis phyllosoma larva halotolerANCE
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Damage Action of Alkali-resistant Glass Fiber in Cement-based Material
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作者 吴会军 ZHAO Jing +1 位作者 WANG Zhongchang SONG Ting 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2013年第4期761-765,共5页
The compressive strength and flexural strength with the same strength class cement mortar of the alkali-resistant glass fiber cement mortar were tested in standard and hot-water curing condition, and the damage mechan... The compressive strength and flexural strength with the same strength class cement mortar of the alkali-resistant glass fiber cement mortar were tested in standard and hot-water curing condition, and the damage mechanism of alkali-resistant glass fiber was studied. The interaction mechanisms of the chemical erosion and physical injury in different curing conditions were studied in order to summarize the damage mechanism of alkali-resistant glass fiber in cement-based materials, and chloride diffusivity coefficient and porosity of cement mortar were tested in the different curing conditions. The experimental results are that the strength of cement based materials and fiber cement slurry interface zone were closely related, and heat curing could accelerate the hydration of cement, but inevitably enlarge the defect. 展开更多
关键词 alkali-resistant glass fiber cement-based materials DAMAGE heat curing condition MICRO-STRUCTURE
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Isolation and Identification of Alkali-Resistant 1,3-Propanediol Producing Strain
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作者 Zhifang Zhao Caifang Wen +3 位作者 Guang Rong Ruiqing Liu Jianguo Xu Qingping Hu 《Advances in Microbiology》 2016年第12期917-926,共11页
1,3-Propanediol is a promising renewable resource produced by microbial production. It is mainly used in many synthetic reactions, particularly applied to the polymer synthesis and cosmetics industry. We described her... 1,3-Propanediol is a promising renewable resource produced by microbial production. It is mainly used in many synthetic reactions, particularly applied to the polymer synthesis and cosmetics industry. We described here the isolation of strain ZH-1, which has the ability of high production with 1,3-propanediol, from Fenhe River in China. It was classified as a member of K. pneumoniae after the study of phenotypic, physio-logical, biochemical and phylogenetic (16S rDNA). The initial glycerol concentration, fermentation time and pH value of strain ZH-1 were determined to be 50 g·L<sup>-1</sup>, 36 h and 8.0. Under these conditions, the practical yield of 1,3-PD was 18.53 g·L<sup>-1</sup> and a molar yield (mol<sub>1,3-PD</sub> mol<sub>Glycerol</sub>-1</sup> of 1,3-propanediol to glycerol of 0.497. In addition, we found that for the strain ZH-1, the optimum grown pH was 9.0, so we can deter-mine that it is a new member of alkali-resistant strains. 展开更多
关键词 1 3-Propanediol K. pneumoniae Identification alkali-resistant
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Effect of salinization on soil properties and mechanisms beneficial to microorganisms in salinized soil remediation-a review
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作者 Jing Pan Xian Xue +6 位作者 CuiHua Huang QuanGang You PingLin Guo RuiQi Yang FuWen Da ZhenWei Duan Fei Peng 《Research in Cold and Arid Regions》 CSCD 2024年第3期121-128,共8页
Salinized soil is an important reserved arable land resource in China.The management and utilization of salinized soil can safeguard the current size of arable land and a stable grain yield.Salt accumulation will lead... Salinized soil is an important reserved arable land resource in China.The management and utilization of salinized soil can safeguard the current size of arable land and a stable grain yield.Salt accumulation will lead to the deterioration of soil properties,destroy soil production potential and damage soil ecological functions,which in turn will threaten global water and soil resources and food security,and affect sustainable socio-economic development.Microorganisms are important components of salinized soil.Microbial remediation is an important research tool in improving salinized soil and is key to realizing sustainable development of agriculture and the ecosystem.Knowledge about the impact of salinization on soil properties and measures using microorganisms in remediation of salinized soil has grown over time.However,the mechanisms governing these impacts and the ecological principles for microbial remediation are scarce.Thus,it is imperative to summarize the effects of salinization on soil physical,chemical,and microbial properties,and then review the related mechanisms of halophilic and halotolerant microorganisms in salinized soil remediation via direct and indirect pathways.The stability,persistence,and safety of the microbial remediation effect is also highlighted in this review to further promote the application of microbial remediation in salinized soil.The objective of this review is to provide reference and theoretical support for the improvement and utilization of salinized soil. 展开更多
关键词 Salinized soil Microbial remediation Halophilic and halotolerant microorganisms Soil properties
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青藏高原尕斯库勒盐湖可培养嗜盐耐盐微生物的群落结构与分离方法对比研究
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作者 马欣 马想蓉 +3 位作者 朱德锐 李轩领 沈国平 邢江娃 《海洋与湖沼》 CAS CSCD 北大核心 2024年第4期916-930,共15页
嗜盐耐盐微生物在生物医药、生物酶等领域有着重要的应用潜力。为更好地利用青藏高原的盐湖微生物资源,以青藏高原尕斯库勒盐湖为研究对象,借助Illumina MiSeq测序平台分析盐湖微生物的群落组成,并采用寡营养培养、富集培养和梯度稀释... 嗜盐耐盐微生物在生物医药、生物酶等领域有着重要的应用潜力。为更好地利用青藏高原的盐湖微生物资源,以青藏高原尕斯库勒盐湖为研究对象,借助Illumina MiSeq测序平台分析盐湖微生物的群落组成,并采用寡营养培养、富集培养和梯度稀释等方法对嗜盐耐盐微生物进行大规模分离与培养。结果表明,高通量测序获得明确分类地位的细菌有30门50纲81目162科417属,古菌6门7纲9目15科39属。细菌优势属类群以矿生菌属(Fodinibius)和纤维单胞菌属(Gracilimonas)为主。古菌优势属类群以盐红菌属(Halorubrum)为主;纯培养法共获得502株嗜盐耐盐微生物,其中嗜盐耐盐细菌472株,分布在4门5纲7目9科13属,优势属为芽孢杆菌属(Bacillus)和盐单胞菌属(Halomonas),其中巴纽尔斯菌(Balneolota)门Fodinibius属可培养细菌是首次从盐湖中分离得到;嗜盐古菌30株,分布在1门1纲1目3科5属,优势属为Halorubrum。对比不同培养条件下的分离结果发现,1/2 TSA培养基和1/2 RCA培养基更有利于嗜盐耐盐细菌的分离,最佳富集天数为7天和30天,稀释梯度10-1和10-2可与未稀释样本互为补充来增加可培养嗜盐耐盐细菌的多样性;1/102216E培养基更适合于古菌的分离,最佳富集天数为7天和14天,且样本不稀释分离效果更好。研究结果可为青藏高原盐湖可培养微生物的分离方案优化提供理论依据和数据支撑。 展开更多
关键词 尕斯库勒盐湖 嗜盐耐盐微生物 分离培养 生物多样性 高盐环境
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微藻盐胁迫响应分子机制研究进展
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作者 沈天虹 齐孝博 +1 位作者 赵瑞丰 马欣荣 《生物技术通报》 CAS CSCD 北大核心 2024年第3期89-99,共11页
微藻是地球上光合微生物的原始种类之一,由于其生长周期较短、生长速率较快和生产高附加值产物的潜力而被广泛开发利用。然而,在微藻放大生产的过程中极易受到高盐等非生物胁迫的不利影响,极大地限制了微藻的生产力。因此,了解微藻盐胁... 微藻是地球上光合微生物的原始种类之一,由于其生长周期较短、生长速率较快和生产高附加值产物的潜力而被广泛开发利用。然而,在微藻放大生产的过程中极易受到高盐等非生物胁迫的不利影响,极大地限制了微藻的生产力。因此,了解微藻盐胁迫响应的分子机制将有助于耐盐藻株的快速建立。本文总结了真核微藻和原核蓝藻响应盐胁迫的各种参与蛋白及其具体作用机制,包括转运蛋白维持离子稳态、积累渗透调节物质、抗氧化防御机制、信号蛋白和脂质积累等;同时综述了已被开发利用的天然耐盐藻包括杜氏盐藻(Dunaliella salina)、盐生隐杆藻(Aphanothece halophytica)、皮克绿球藻(Picochlorum sp.)和海洋绿藻(Chlamydomonas W80)等微藻及其耐盐基因的研究进展;最后讨论了典型盐响应基因在优良藻种选育中的价值与应用前景。 展开更多
关键词 真核微藻 原核蓝藻 盐胁迫 耐盐藻 盐胁迫响应分子机制
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耐盐微生物对鯷制鱼露风味形成的影响 被引量:14
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作者 江津津 曾庆孝 +2 位作者 朱志伟 晁岱秀 颜楠楠 《食品与发酵工业》 CAS CSCD 北大核心 2008年第11期25-28,共4页
研究了鯷制鱼露中的耐盐微生物(耐盐乳酸菌M1、M2和耐盐产蛋白酶菌T1)对其发酵初期挥发性风味物质形成的影响。将从鱼露发酵液中分离出来的耐盐微生物经过扩大培养后添加到鱼盐混合物中进行鱼露发酵。在发酵初期,总酸、氨基酸态氮、总... 研究了鯷制鱼露中的耐盐微生物(耐盐乳酸菌M1、M2和耐盐产蛋白酶菌T1)对其发酵初期挥发性风味物质形成的影响。将从鱼露发酵液中分离出来的耐盐微生物经过扩大培养后添加到鱼盐混合物中进行鱼露发酵。在发酵初期,总酸、氨基酸态氮、总氮、非酶褐变指数等理化指标都明显优于未添加发酵剂的空白对照鱼露发酵液。用气相色谱-质谱联用(GC-MS)分析其挥发性风味物质。结果表明,鱼露初期发酵液中分离出来的耐盐乳酸菌M2、产蛋白酶菌T1对鱼露挥发性特征风味成分的形成,尤其是挥发性酸的形成有积极的贡献,而自然发酵的鱼露则很难在发酵初期产生特征挥发性风味物质。 展开更多
关键词 鱼露 挥发性风味 耐盐乳酸菌 耐盐产蛋白酶菌
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一株耐盐菌的分离及其降解特性 被引量:16
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作者 王丽娟 钱子雯 +4 位作者 沈海波 朱君 吴彦霏 陈孟君 王利群 《化工进展》 EI CAS CSCD 北大核心 2017年第3期1047-1051,共5页
煤化工废水是一种含盐量较高的难降解工业废水,为了提高其生化处理的效果,从某煤化工企业生产污水中分离得到一株耐盐菌CCZU-R6,经形态观察和16S rDNA序列分析,鉴定该菌株属于芽孢杆菌属(Bacillus sp.)。采用单因素实验方法考察了pH、... 煤化工废水是一种含盐量较高的难降解工业废水,为了提高其生化处理的效果,从某煤化工企业生产污水中分离得到一株耐盐菌CCZU-R6,经形态观察和16S rDNA序列分析,鉴定该菌株属于芽孢杆菌属(Bacillus sp.)。采用单因素实验方法考察了pH、温度、接种量、废水盐度等工艺条件对降解模拟含盐废水的影响,确定了菌株CCZU-R6处理模拟含盐废水的最适条件为pH 7.0、温度35℃,接种量500mg/L,能耐受的最高盐度约为11%。底物谱分析显示菌株CCZU-R6对苯酚、苯甲醇、甲醇等物质具有显著的降解效果。在最适降解条件下,菌株CCZU-R6处理实际煤化工含盐废水24h,COD去除率达到93%。表明该菌株对含盐煤化工废水有较强的降解能力,具有工业应用前景。 展开更多
关键词 耐盐菌 分离 降解 含盐废水
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MBR在垃圾渗滤液处理中的应用研究 被引量:11
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作者 季民 杨拓 +3 位作者 张亮 李征 霍贞 刘卫华 《给水排水》 CSCD 北大核心 2007年第9期47-52,共6页
对比研究了UASB-MBAC工艺、普通MBR工艺及投加专性耐盐菌强化MBR工艺处理垃圾渗滤液,特别是对高含盐垃圾渗滤液的效果。结果表明:采用普通MBR处理高含盐垃圾渗滤液时,对有机物去除能力有限,运行时间越长,处理效率越低;投加耐盐菌可有效... 对比研究了UASB-MBAC工艺、普通MBR工艺及投加专性耐盐菌强化MBR工艺处理垃圾渗滤液,特别是对高含盐垃圾渗滤液的效果。结果表明:采用普通MBR处理高含盐垃圾渗滤液时,对有机物去除能力有限,运行时间越长,处理效率越低;投加耐盐菌可有效改善MBR对高含盐渗滤液中有机物的去除效果。试验发现不论是普通MBR还是投加耐盐菌强化的MBR处理高含盐渗滤液时,对氨氮都具有较高的去除率,几乎达到100%。 展开更多
关键词 膜生物反应器 垃圾渗滤液 耐盐菌
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枯草芽孢杆菌渗透压调节基因proB的克隆和表达 被引量:20
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作者 张小青 曹军卫 +1 位作者 翟超 陈建军 《微生物学报》 CAS CSCD 北大核心 2002年第2期163-168,共6页
用PCR扩增的方法从耐盐的枯草杆菌中克隆出一个 1 3kb长的DNA片段 ,经功能检测 ,证明正向插入片段与大肠杆菌的脯氨酸营养缺陷特性 (proB- )能够营养互补。含有该重组质粒的大肠杆菌DH5α在基本培养基上的耐盐能力从 2 %提高至 4%。通... 用PCR扩增的方法从耐盐的枯草杆菌中克隆出一个 1 3kb长的DNA片段 ,经功能检测 ,证明正向插入片段与大肠杆菌的脯氨酸营养缺陷特性 (proB- )能够营养互补。含有该重组质粒的大肠杆菌DH5α在基本培养基上的耐盐能力从 2 %提高至 4%。通过引物步行法测定了该插入片段的核苷酸序列。利用DNAsis软件进行序列分析发现 ,该片段第 1 2 2~ 1 2 3 5bp核苷酸编码一个由 3 70个氨基酸组成的蛋白质分子 ,其上游存在非典型的 - 1 0区 ,典型的 -3 5区和核糖体结合位点 ,起始密码子处有最佳翻译起始效率的侧翼核苷酸序列。将其与Genebank中的已知基因的序列和编码的氨基酸序列进行同源性比较 ,结果表明该片段与枯草杆菌 1 6 8的核苷酸序列、氨基酸序列的同源性分别为 81 %和 90 %。证明该基因确实是一个proB基因。通过与三十个不同种属微芽生物proB基因的氨基酸序列比较 。 展开更多
关键词 耐盐枯草芽孢杆菌 proB基因 克隆 序列分析 表达 渗透压调节基因
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中国龙虾叶状幼体的盐度耐受力 被引量:6
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作者 陈昌生 陈政强 +3 位作者 胡家财 叶兆弘 纪德华 蓝良纯 《海洋学报》 CAS CSCD 北大核心 2003年第S2期24-28,共5页
用盐度为13,15,19,23,27,31,35,39,43,47,49等多个梯度的海水,对中国龙虾叶状幼体进行盐度耐受力试验.当盐度从31逐渐升高至49或降低至13时,叶状幼体可生存4~6d,成活率为25 0%~47 5%.在盐度为23~39范围内,叶状幼体能正常生长、发育... 用盐度为13,15,19,23,27,31,35,39,43,47,49等多个梯度的海水,对中国龙虾叶状幼体进行盐度耐受力试验.当盐度从31逐渐升高至49或降低至13时,叶状幼体可生存4~6d,成活率为25 0%~47 5%.在盐度为23~39范围内,叶状幼体能正常生长、发育和变态,其中盐度为31时,叶状幼体Ⅱ,Ⅲ期的变态率分别为85%和48%;叶状幼体在不同盐度海水的水层中分布呈现一定规律,低盐组的叶状幼体,主要分布在水体的下层,高盐组的叶状幼体多数分布在水体的上、中层.试验结果表明,中国龙虾叶状幼体忍受盐度变化能力较强,忍受的范围为15~47,其生长发育的适宜盐度为27~35. 展开更多
关键词 中国龙虾 叶状幼体 盐度耐受力
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作物耐盐性的分子生物学研究进展 被引量:56
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作者 李玉全 张海艳 沈法富 《山东科学》 CAS 2002年第2期8-14,共7页
本文从作物耐离子胁迫、耐渗透胁迫和细胞编程性死亡调节三个方面概述了作物耐盐的生理生化机制 ;介绍了目前利用分子生物学技术克隆与植物耐盐相关的基因 ,并根据作物的耐盐机理 ,对这些与耐盐相关的基因进行了分类 ;总结分析了转耐盐... 本文从作物耐离子胁迫、耐渗透胁迫和细胞编程性死亡调节三个方面概述了作物耐盐的生理生化机制 ;介绍了目前利用分子生物学技术克隆与植物耐盐相关的基因 ,并根据作物的耐盐机理 ,对这些与耐盐相关的基因进行了分类 ;总结分析了转耐盐基因植物的研究现状及其转基因植物的耐盐性状况 ,并提出了利用转基因技术 ,改良植物耐盐性的方法和对策。 展开更多
关键词 作物 耐盐性 分子生物学 耐盐机制 耐盐基因 转基因植物
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耐温耐盐深部调剖体系研究 被引量:27
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作者 杨文军 赵光 +3 位作者 刘奎 赵辉 王苹 戴彩丽 《断块油气田》 CAS 北大核心 2011年第2期257-260,共4页
采用非离子聚合物与酚醛树脂制备了一种耐温耐盐的深部调剖剂,并考察了深部调剖剂的成冻性能。根据成冻强度级别的划分,定性地测定了成冻时间,用突破真空度法定量地测定了成冻强度,并绘制出成冻时间和成冻强度等值图。评价了聚合物... 采用非离子聚合物与酚醛树脂制备了一种耐温耐盐的深部调剖剂,并考察了深部调剖剂的成冻性能。根据成冻强度级别的划分,定性地测定了成冻时间,用突破真空度法定量地测定了成冻强度,并绘制出成冻时间和成冻强度等值图。评价了聚合物与交联剂的质量分数、温度、pH值、矿化度及剪切速率对成冻性能的影响,也评价了油井的封堵性能,并分析了注入时机对采收率的影响。结果表明:交联体系成冻时间在1~18d可调,成冻后强度高,具有很好的耐温耐盐性;pH值适用范围广,抗剪切性强,封堵性能好。在注入相同量的调剖剂时,注入时机越早,提高采收率增值也就越大。 展开更多
关键词 耐温耐盐 深部调剖 冻胶 非离子聚合物 注入时机
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一株耐温耐盐烃降解菌Geobacillus sp. XDF-4性能 被引量:12
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作者 夏文杰 董汉平 +2 位作者 俞理 黄立信 赵婷 《化工学报》 EI CAS CSCD 北大核心 2010年第11期2951-2959,共9页
从大庆油田龙虎泡区块采油地层水中分离得到一株性能很好的耐盐耐温的兼性烃降解菌XDF-4,经形态观察、生理生化实验和16S rDNA基因序列分析,初步鉴定为地芽孢杆菌Geobacillus sp.。该菌在45~75℃、pH6.5~9.0、盐的质量分数0~10%下生长良... 从大庆油田龙虎泡区块采油地层水中分离得到一株性能很好的耐盐耐温的兼性烃降解菌XDF-4,经形态观察、生理生化实验和16S rDNA基因序列分析,初步鉴定为地芽孢杆菌Geobacillus sp.。该菌在45~75℃、pH6.5~9.0、盐的质量分数0~10%下生长良好,其最适生长温度为65℃,最适盐的质量分数为3.0%。研究发现,该菌株能以原油为唯一碳源生长并合成生物表面活性剂,发酵7d,其发酵液表面张力从68.59mN.m-1降到29.58mN.m-1。薄层色谱和显色反应表明,XDF-4产出的表面活性剂主要包含:糖类50.26%(质量)、脂类28.47%(质量)、蛋白质15.35%(质量);其临界胶束浓度为22mg·mL-1。GC气相色谱和族组分柱色谱分析表明,烃降解菌Geobacillussp.XDF-4作用后,原油轻质组分含量明显增加,重质组分含量降低。物理模拟实验表明,该菌可在一次水驱基础上进一步提高采收率5.69%,可有效应用于高温高盐油藏微生物驱现场实验。 展开更多
关键词 耐温耐盐 表面张力 生物表面活性剂 族组分 物理模拟 采收率
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一株耐盐菌对苯酚的降解及其影响因素研究 被引量:4
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作者 黄中子 李辉 +2 位作者 刘勇弟 陆志艳 郭晓觉 《水处理技术》 CAS CSCD 北大核心 2015年第6期52-56,共5页
从青海察尔汗盐湖的沉积物中分离出1株耐盐苯酚降解菌,经16S rRNA序列分析鉴定为Brachybacterium sp.PDB-G1(Gen Bank序列号为KM658980)。该菌株在8%的总盐度下最高能耐受质量浓度1 200 mg/L的苯酚,在72 h内对初始质量浓度为500 mg/L... 从青海察尔汗盐湖的沉积物中分离出1株耐盐苯酚降解菌,经16S rRNA序列分析鉴定为Brachybacterium sp.PDB-G1(Gen Bank序列号为KM658980)。该菌株在8%的总盐度下最高能耐受质量浓度1 200 mg/L的苯酚,在72 h内对初始质量浓度为500 mg/L苯酚的降解率可达90%以上。PDB-G1可在5%~12%的高盐条件下有效去除高含量苯酚。培养温度和p H对菌株PDB-G1生长和苯酚降解率具有较大影响,其适宜生长和降解温度为30℃、p H为7.0~7.5。PDB-G1具有盐适应范围广、耐盐含量高、苯酚降解能力强及降解速率快等优点,在高盐含酚废水生物处理中具有较大的应用潜力。 展开更多
关键词 苯酚 生物降解 耐盐菌 短杆菌属
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盐碱土壤中耐盐细菌的分离与鉴定 被引量:18
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作者 张广志 周红姿 +1 位作者 杨合同 赵晓燕 《山东农业科学》 2008年第9期49-50,54,共3页
从盐碱土壤中分离筛选耐盐菌株12株,经鉴定分别为假单胞菌属(Pseudomonas),黄杆菌属(Flavobacterium),盐单胞菌属(Halomonas),微球菌属(Micrococcus)和芽孢杆菌(Bacillus)。各菌株均耐盐至10%,且对小麦、番茄、辣椒和黄瓜没有潜在致病性。
关键词 耐盐细菌 分离 鉴定 耐盐性
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