Assessment of railway wheel slide protection(WSP) systems involves the execution of complex experimental activities that are quite expensive and time-consuming, since they involve the physical reproduction of degraded...Assessment of railway wheel slide protection(WSP) systems involves the execution of complex experimental activities that are quite expensive and time-consuming, since they involve the physical reproduction of degraded adhesion conditions on a real railway line. WSP is devoted to regulating applied braking forces to avoid excessive wheel sliding in case of degraded adhesion conditions between wheel and rail. WSP must be also compliant to safety specifications related to assured braking performances and consumed air. Hardware in the loop(HIL) testing offers an affordable and sustainable way to accelerate these activities optimizing cost, duration and safety of experimental activities performed online. HIL test rigs are subjected to continuous updates, customization and natural ageing of their components. This work investigates the criteria that can be adopted to assure a continuous monitoring and validation of a real WSP test rig, the Italian test rig of Firenze Osmannoro.展开更多
目的探讨中国流行株 HIV- 1gag与 h IL - 2 /h IL - 6共表达重组核酸疫苗质粒的免疫效果。方法以核酸疫苗质粒 p IRES1neo为表达载体 ,构建重组核酸疫苗质粒 p IRES1- gag、p IRES1- gag- h IL- 2、p IRES1- gag- h IL- 6 ,通过间接免...目的探讨中国流行株 HIV- 1gag与 h IL - 2 /h IL - 6共表达重组核酸疫苗质粒的免疫效果。方法以核酸疫苗质粒 p IRES1neo为表达载体 ,构建重组核酸疫苗质粒 p IRES1- gag、p IRES1- gag- h IL- 2、p IRES1- gag- h IL- 6 ,通过间接免疫荧光试验、Dot- EL ISA检测 gag/h IL - 2 /h IL - 6基因的表达产物。另将此重组核酸疫苗质粒免疫 Balb/c小鼠 ,进行淋巴细胞转化试验、CD4+ 、CD8+ T淋巴细胞数量测定、细胞毒性 T淋巴细胞 (CTL )特异性杀伤作用检测及血清抗体检测 ,结果构建的重组质粒转染 BHK细胞后可表达目的基因 ,免疫小鼠后可有效地刺激淋巴细胞增殖、诱导特异性 CTL 反应 ,当和 h IL- 2 /h IL- 6共表达时免疫效果更加显著。讨论与 Gag蛋白共表达的 h IL- 2 /h IL- 6能够进一步增强免疫鼠的细胞免疫与体液免疫水平 ,构建的重组质粒为 HIV-展开更多
文摘Assessment of railway wheel slide protection(WSP) systems involves the execution of complex experimental activities that are quite expensive and time-consuming, since they involve the physical reproduction of degraded adhesion conditions on a real railway line. WSP is devoted to regulating applied braking forces to avoid excessive wheel sliding in case of degraded adhesion conditions between wheel and rail. WSP must be also compliant to safety specifications related to assured braking performances and consumed air. Hardware in the loop(HIL) testing offers an affordable and sustainable way to accelerate these activities optimizing cost, duration and safety of experimental activities performed online. HIL test rigs are subjected to continuous updates, customization and natural ageing of their components. This work investigates the criteria that can be adopted to assure a continuous monitoring and validation of a real WSP test rig, the Italian test rig of Firenze Osmannoro.
