目的探讨转化生长因子β1(transforming growth factor-β1,TGF-β1)和iRoot BP Plus联合盖髓的可行性,以期为提高活髓保存成功率提供实验参考。方法获取4周龄雄性SD大鼠的上、下颌第一磨牙共40颗,截根,保留第一磨牙牙冠及冠髓组织,随...目的探讨转化生长因子β1(transforming growth factor-β1,TGF-β1)和iRoot BP Plus联合盖髓的可行性,以期为提高活髓保存成功率提供实验参考。方法获取4周龄雄性SD大鼠的上、下颌第一磨牙共40颗,截根,保留第一磨牙牙冠及冠髓组织,随机分为4组进行盖髓:对照组、iRoot BP Plus组、TGF-β1组、iRoot BP Plus+TGF-β1组。将盖髓术后的实验牙样本移植入6周龄雄性BALB/c裸鼠背部两侧的囊袋中。培养6周后,处死裸鼠并取出样本,进行HE染色,分析评价样本牙髓炎症反应程度和修复性牙本质或矿化组织形成情况;免疫组织化学检测牙本质基质蛋白1(dentin matrix protein 1,DMP-1)在成牙本质细胞中的表达情况。结果iRoot BP Plus组、TGF-β1组、iRoot BP Plus+TGF-β1组牙髓炎症反应程度、修复性牙本质或矿化组织形成程度优于对照组(P<0.05);iRoot BP Plus+TGF-β1组修复性牙本质或矿化组织形成程度明显优于iRoot BP Plus组、TGF-β1组(P<0.05)。iRoot BP Plus组、TGF-β1组、iRoot BP Plus+TGF-β1组DMP-1在成牙本质细胞中的表达明显强于对照组,iRoot BP Plus+TGF-β1组DMP-1在成牙本质细胞中的表达明显强于iRoot BP Plus组和TGF-β1组(P<0.05)。结论iRoot BP Plus、TGF-β1均能促进修复性牙本质或矿化组织的形成,iRoot BP Plus与TGF-β1联合应用于直接盖髓术时,形成修复性牙本质或矿化组织的能力优于单独使用iRoot BP Plus或TGF-β1。展开更多
The tasseled cap transformation of remote sensing data has been widely used in agriculture,forest,ecology,and landscape.In this paper,tasseled cap transformation coefficients appropriate for data from a new sensor(Chi...The tasseled cap transformation of remote sensing data has been widely used in agriculture,forest,ecology,and landscape.In this paper,tasseled cap transformation coefficients appropriate for data from a new sensor(China & Brazil Earth Resource Satellite(CBERS-02B)) are presented.The first three components after transformation captured 98% of the four-band variance,and represent the physical characteristics of brightness(coefficients:0.509,0.431,0.330,and 0.668),greenness(coefficients:0.494,0.318,0.324,and 0.741),and blueness(coefficients:0.581,0.070,0.811,and 0.003),respectively.We hope these results will enhance the application of CBERS-02B charge-coupled device(CCD) data in the areas of agriculture,forest,ecology,and landscape.展开更多
文摘目的探讨转化生长因子β1(transforming growth factor-β1,TGF-β1)和iRoot BP Plus联合盖髓的可行性,以期为提高活髓保存成功率提供实验参考。方法获取4周龄雄性SD大鼠的上、下颌第一磨牙共40颗,截根,保留第一磨牙牙冠及冠髓组织,随机分为4组进行盖髓:对照组、iRoot BP Plus组、TGF-β1组、iRoot BP Plus+TGF-β1组。将盖髓术后的实验牙样本移植入6周龄雄性BALB/c裸鼠背部两侧的囊袋中。培养6周后,处死裸鼠并取出样本,进行HE染色,分析评价样本牙髓炎症反应程度和修复性牙本质或矿化组织形成情况;免疫组织化学检测牙本质基质蛋白1(dentin matrix protein 1,DMP-1)在成牙本质细胞中的表达情况。结果iRoot BP Plus组、TGF-β1组、iRoot BP Plus+TGF-β1组牙髓炎症反应程度、修复性牙本质或矿化组织形成程度优于对照组(P<0.05);iRoot BP Plus+TGF-β1组修复性牙本质或矿化组织形成程度明显优于iRoot BP Plus组、TGF-β1组(P<0.05)。iRoot BP Plus组、TGF-β1组、iRoot BP Plus+TGF-β1组DMP-1在成牙本质细胞中的表达明显强于对照组,iRoot BP Plus+TGF-β1组DMP-1在成牙本质细胞中的表达明显强于iRoot BP Plus组和TGF-β1组(P<0.05)。结论iRoot BP Plus、TGF-β1均能促进修复性牙本质或矿化组织的形成,iRoot BP Plus与TGF-β1联合应用于直接盖髓术时,形成修复性牙本质或矿化组织的能力优于单独使用iRoot BP Plus或TGF-β1。
基金Project (No. 40871158/D0106) supported by the National Natural Science Foundation of China
文摘The tasseled cap transformation of remote sensing data has been widely used in agriculture,forest,ecology,and landscape.In this paper,tasseled cap transformation coefficients appropriate for data from a new sensor(China & Brazil Earth Resource Satellite(CBERS-02B)) are presented.The first three components after transformation captured 98% of the four-band variance,and represent the physical characteristics of brightness(coefficients:0.509,0.431,0.330,and 0.668),greenness(coefficients:0.494,0.318,0.324,and 0.741),and blueness(coefficients:0.581,0.070,0.811,and 0.003),respectively.We hope these results will enhance the application of CBERS-02B charge-coupled device(CCD) data in the areas of agriculture,forest,ecology,and landscape.