Objective:This study was designed to explore the characteristics of gut microbiota in CHB patients with two most common traditional Chinese medicine(TCM)Syndromes—damp heat syndrome and liver depression and spleen de...Objective:This study was designed to explore the characteristics of gut microbiota in CHB patients with two most common traditional Chinese medicine(TCM)Syndromes—damp heat syndrome and liver depression and spleen deficiency syndrome.Methods:According to the diagnostic criteria of Western medicine,TCM and screening exclusion criteria,65 cases of CHB with damp heat syndrome and 28 cases of CHB with liver depression and spleen deficiency syndrome were finally included in the study.All the basic information was gathered and the fresh fecal samples were collected for 16S rDNA sequencing.16S rDNA of gut microbiota was sequenced using Illumina hiseq 2,500 high-throughput sequencing platform.Based on the optimized sequence,Operational Taxonomic Units(OTU)clustering analysis and taxonomic annotation were carried out.Results:The difference in relative abundance of gut microbiota was significant between damp heat syndrome and liver depression and spleen deficiency syndrome in CHB patients.Cyanobacteria was only found in damp heat syndrome.The relative abundance of Erysipelotrichia and Subdoligranulum were higher in liver depression and spleen deficiency syndrome,while the relative abundance of Rhodospirillales,Alphaproteobacteria and Lachnospira were higher in the damp heat syndrome.LDA Effect Size(LEfSe)analysis showed that Lachnospira,Olsenella and Subdoligranulum had significant difference in species among the two TCM syndromes.Conclusion:The different characteristics of gut microbiota in the two TCM syndromes of CHB patients may play an important role in syndrome formation of TCM,which provides a new field of vision for the accurate diagnosis and treatment of TCM.展开更多
目的观察濬川散联合自拟更衣贴治疗便秘型肠易激综合征(Irritable bowel syndrome with constipation,IBS-C)胃肠积热证的临床疗效。方法将100例IBS-C患者随机分为濬川散组(50例)和西药组(50例)。西药组口服枸橼酸莫沙必利片;濬川散组...目的观察濬川散联合自拟更衣贴治疗便秘型肠易激综合征(Irritable bowel syndrome with constipation,IBS-C)胃肠积热证的临床疗效。方法将100例IBS-C患者随机分为濬川散组(50例)和西药组(50例)。西药组口服枸橼酸莫沙必利片;濬川散组口服濬川散,外敷自拟更衣贴治疗。2组治疗20 d后比较相关指标。结果濬川散组总有效率明显高于西药组(P<0.05);治疗后,2组血清胃动力指标较治疗前升高,且濬川散组高于西药组(P<0.05);治疗后,濬川散组肠鸣音频率更高(P<0.05),而首次排便时间更短,便秘症状积分、心理应激程度评分(IBS-QOL)均低于西药组(均P<0.05);濬川散组复发率更低(P<0.05)。结论应用濬川散联合自拟更衣贴可有效改善IBS-C症状,促进胃功能恢复。展开更多
基金The Sixth Group of National Senior Chinese Medicine Experts’ Academic Experience Inheritance Project [approval:State Administration of Traditional Chinese Medicine (2017) No.29]the Foshan High Level Medical Key Discipline Construction Project and "Peak Climbing Plan" for Foshan High Level Hospital Construction[approval:Foshan Municipal Government Office,No. 2019 (01)]
文摘Objective:This study was designed to explore the characteristics of gut microbiota in CHB patients with two most common traditional Chinese medicine(TCM)Syndromes—damp heat syndrome and liver depression and spleen deficiency syndrome.Methods:According to the diagnostic criteria of Western medicine,TCM and screening exclusion criteria,65 cases of CHB with damp heat syndrome and 28 cases of CHB with liver depression and spleen deficiency syndrome were finally included in the study.All the basic information was gathered and the fresh fecal samples were collected for 16S rDNA sequencing.16S rDNA of gut microbiota was sequenced using Illumina hiseq 2,500 high-throughput sequencing platform.Based on the optimized sequence,Operational Taxonomic Units(OTU)clustering analysis and taxonomic annotation were carried out.Results:The difference in relative abundance of gut microbiota was significant between damp heat syndrome and liver depression and spleen deficiency syndrome in CHB patients.Cyanobacteria was only found in damp heat syndrome.The relative abundance of Erysipelotrichia and Subdoligranulum were higher in liver depression and spleen deficiency syndrome,while the relative abundance of Rhodospirillales,Alphaproteobacteria and Lachnospira were higher in the damp heat syndrome.LDA Effect Size(LEfSe)analysis showed that Lachnospira,Olsenella and Subdoligranulum had significant difference in species among the two TCM syndromes.Conclusion:The different characteristics of gut microbiota in the two TCM syndromes of CHB patients may play an important role in syndrome formation of TCM,which provides a new field of vision for the accurate diagnosis and treatment of TCM.
