Recently we are witnessing the boom of high-pressure science and technology from a small niche field to becoming a major dimension in physical sciences.One of the most important technological advances is the integrati...Recently we are witnessing the boom of high-pressure science and technology from a small niche field to becoming a major dimension in physical sciences.One of the most important technological advances is the integration of synchrotron nanotechnology with the minute samples at ultrahigh pressures.Applications of high pressure have greatly enhanced our understanding of the electronic,phonon,and doping effects on the newly emerged graphene and related 2D layered materials.High pressure has created exotic stoichiometry even in common Group 17,15,and 14 compounds and drastically altered the basic σ and π bonding of organic compounds.Differential pressure measurements enable us to study the rheology and flow of mantle minerals in solid state,thus quantitatively constraining the geodynamics.They also introduce a new approach to understand defect and plastic deformations of nano particles.These examples open new frontiers of high-pressure research.展开更多
Based on rpoB gene micro array as target gene, we are going to use gene chip technology to test 24 mycobacterium standard specimens, 8 non-mycobacterium specimens and 86 mycobacterium clinical isolated specimens. As a...Based on rpoB gene micro array as target gene, we are going to use gene chip technology to test 24 mycobacterium standard specimens, 8 non-mycobacterium specimens and 86 mycobacterium clinical isolated specimens. As a result, after mycobacterium and non-mycobacterium standard specimens were duplicated by PCR, mycobacterium standard specimens reproduced 360bp DNA fragments; on the other hand, non-mycobacterium specimens did not reproduce any fragments except for hemolytic streptococcus and corynebacterium pseudodiphtheriticum which had the same results as mycobacterium standard specimens. Sensitive test is able to detect lpg tuberculosis mycobacterium DNA. The probe test showed that, among 21 oligonucleotide probes, probe-M. fortuitum and M. marinum were cross-hybrid; the other probes were specific. We used the new method to identify 126 mycobacterium clinical isolated specimens. The test results of this new method matched with conventional method. In conclusion, compared to the traditional method, the use of rpob gene chip technology to identify mycobacterium species will be faster, more accurate and higher value in application.展开更多
Handover location technology was employed for collecting road traffic information in a number of field projects, and the project results demonstrate that it is a supplementary and promising means of road traffic infor...Handover location technology was employed for collecting road traffic information in a number of field projects, and the project results demonstrate that it is a supplementary and promising means of road traffic information collection for further traffic supervision and maintenance. Because handover location technology is one kind of pattern matching based location technologies, offiine handovex location positioning is an essential problem to be studied for successfully matching mobile probes on GIS map. Offline handover location positioning method involves two stages, handover location positioning respectively via two weighted models and an optimized model based on the intermediate results obtained in the first stage. A preliminary field test is conducted on a stretch of freeway in the inner suburban region in Beijing, and performance evaluation results show that the proposed method is superior to standard least square model in location accuracy and location precision, which is an effective method of offline handover location positioning.展开更多
Because the UT （ultrasonic testing） flexible probe technology may be an appropriate answer to examine components with uneven surface, AREVA has developed an industrial application of the CEA＇s （French Atomic Energ...Because the UT （ultrasonic testing） flexible probe technology may be an appropriate answer to examine components with uneven surface, AREVA has developed an industrial application of the CEA＇s （French Atomic Energy and Alternative Energies） flexible phased arrays sensors. As a ＂first of a kind＂ project, the challenges faced were significant, including developing a phased array smart probe suitable for industrial use on rather simple but large scale geometries, permitting UT propagation within a constraining media structure and then targeting a qualification according to ENIQ （European Network for Inspection Qualification） methodology. A prototype flexible probe, designed for UT validation, and final flexible linear array probes permitting the UT behavior （as, e.g., detection and sizing from diffraction type echoes） to be maintained on wavy coupling surfaces, have been manufactured. These probes include a profilemeter with optical sensors control and a specifically designed coupling circuit （avoiding probe housing tightness issues）. Qualification has been performed using open test blocks, （where known ＂defects＂ exist, for procedure qualification）, and blind test blocks, （where ＂defects＂ are unknown, for qualification of testing personnel）. One open test bloc was customized to represent a ＂real＂ surface condition, with gaps up to 2.5 mm under the regular rigid probes. AREVAI/BGSI in Germany was selected to lead the project, with assistance in development and manufacturing sub-contracted to ＂CEA/LIST＂ laboratory, and the companies ＂IMASONIC＂ and ＂M2M＂. This paper describes the development of these probes and explains a few features （ENIQ qualification objectives fulfilled, UT data acquired on actual perturbed surface） that made their industrial implementation successful.展开更多
文摘Recently we are witnessing the boom of high-pressure science and technology from a small niche field to becoming a major dimension in physical sciences.One of the most important technological advances is the integration of synchrotron nanotechnology with the minute samples at ultrahigh pressures.Applications of high pressure have greatly enhanced our understanding of the electronic,phonon,and doping effects on the newly emerged graphene and related 2D layered materials.High pressure has created exotic stoichiometry even in common Group 17,15,and 14 compounds and drastically altered the basic σ and π bonding of organic compounds.Differential pressure measurements enable us to study the rheology and flow of mantle minerals in solid state,thus quantitatively constraining the geodynamics.They also introduce a new approach to understand defect and plastic deformations of nano particles.These examples open new frontiers of high-pressure research.
文摘Based on rpoB gene micro array as target gene, we are going to use gene chip technology to test 24 mycobacterium standard specimens, 8 non-mycobacterium specimens and 86 mycobacterium clinical isolated specimens. As a result, after mycobacterium and non-mycobacterium standard specimens were duplicated by PCR, mycobacterium standard specimens reproduced 360bp DNA fragments; on the other hand, non-mycobacterium specimens did not reproduce any fragments except for hemolytic streptococcus and corynebacterium pseudodiphtheriticum which had the same results as mycobacterium standard specimens. Sensitive test is able to detect lpg tuberculosis mycobacterium DNA. The probe test showed that, among 21 oligonucleotide probes, probe-M. fortuitum and M. marinum were cross-hybrid; the other probes were specific. We used the new method to identify 126 mycobacterium clinical isolated specimens. The test results of this new method matched with conventional method. In conclusion, compared to the traditional method, the use of rpob gene chip technology to identify mycobacterium species will be faster, more accurate and higher value in application.
基金Projects(71131001,60874078) supported by the National Natural Science Foundation of ChinaProject(NCET-08-0718) supported by Program for New Century Excellent Talents in University of China
文摘Handover location technology was employed for collecting road traffic information in a number of field projects, and the project results demonstrate that it is a supplementary and promising means of road traffic information collection for further traffic supervision and maintenance. Because handover location technology is one kind of pattern matching based location technologies, offiine handovex location positioning is an essential problem to be studied for successfully matching mobile probes on GIS map. Offline handover location positioning method involves two stages, handover location positioning respectively via two weighted models and an optimized model based on the intermediate results obtained in the first stage. A preliminary field test is conducted on a stretch of freeway in the inner suburban region in Beijing, and performance evaluation results show that the proposed method is superior to standard least square model in location accuracy and location precision, which is an effective method of offline handover location positioning.
文摘Because the UT （ultrasonic testing） flexible probe technology may be an appropriate answer to examine components with uneven surface, AREVA has developed an industrial application of the CEA＇s （French Atomic Energy and Alternative Energies） flexible phased arrays sensors. As a ＂first of a kind＂ project, the challenges faced were significant, including developing a phased array smart probe suitable for industrial use on rather simple but large scale geometries, permitting UT propagation within a constraining media structure and then targeting a qualification according to ENIQ （European Network for Inspection Qualification） methodology. A prototype flexible probe, designed for UT validation, and final flexible linear array probes permitting the UT behavior （as, e.g., detection and sizing from diffraction type echoes） to be maintained on wavy coupling surfaces, have been manufactured. These probes include a profilemeter with optical sensors control and a specifically designed coupling circuit （avoiding probe housing tightness issues）. Qualification has been performed using open test blocks, （where known ＂defects＂ exist, for procedure qualification）, and blind test blocks, （where ＂defects＂ are unknown, for qualification of testing personnel）. One open test bloc was customized to represent a ＂real＂ surface condition, with gaps up to 2.5 mm under the regular rigid probes. AREVAI/BGSI in Germany was selected to lead the project, with assistance in development and manufacturing sub-contracted to ＂CEA/LIST＂ laboratory, and the companies ＂IMASONIC＂ and ＂M2M＂. This paper describes the development of these probes and explains a few features （ENIQ qualification objectives fulfilled, UT data acquired on actual perturbed surface） that made their industrial implementation successful.
