In this work the effects of O_2 concentration on the pulsed dielectric barrier discharge in helium-oxygen mixture at atmospheric pressure have been numerically researched by using a one-dimensional fluid model in conj...In this work the effects of O_2 concentration on the pulsed dielectric barrier discharge in helium-oxygen mixture at atmospheric pressure have been numerically researched by using a one-dimensional fluid model in conjunction with the chosen key species and chemical reactions.The reliability of the used model has been examined by comparing the calculated discharge current with the reported experiments. The present work presents the following significant results. The dominative positive and negative particles are He_2~＋ and O_2^-, respectively, the densities of the reactive oxygen species（ROS） get their maxima nearly at the central position of the gap, and the density of the ground state O is highest in the ROS. The increase of O_2 concentration results in increasingly weak discharge and the time lag of the ignition. For O_2 concentrations below 1.1%,the density of O is much higher than other species, the averaged dissipated power density presents an evident increase for small O_2 concentration and then the increase becomes weak. In particular,the total density of the reactive oxygen species reaches its maximums at the O_2 concentration of about 0.5%. This characteristic further convinces the experimental observation that the O_2 concentration of 0.5% is an optimal O_2/He ratio in the inactivation of bacteria and biomolecules when radiated by using the plasmas produced in a helium oxygen mixture.展开更多
OBJECTIVE In order to investigate the possible anti-tumor molecular mechanisms of gecko polypeptide mixture(GPM).METHODS RNA-seq technology was used to identify the differentially expressed genes of human hepatocellul...OBJECTIVE In order to investigate the possible anti-tumor molecular mechanisms of gecko polypeptide mixture(GPM).METHODS RNA-seq technology was used to identify the differentially expressed genes of human hepatocellular carcinoma(HCC)HepG2 cells treated with or without GPM.The HepG2 cells were treated with different concentration of GPM(0,0.1,0.2,0.3,0.4 mg·mL^(-1))for 6 h,12 h and 24 h,respectively.MTT assay was used to detect the viability of HepG2 cells.DAPI fluorescence staining was performed to observe nucleus morphological changes of HepG2 cells.Western blot analysis was applied to observe the expression of apoptosis-related proteins in HepG2 cells.RESULTS The results showed that GPM could induce HepG2 cells apoptosis and influence HepG2 cells proliferation in a dose-dependent manner.We applied many analysis methods,including differentially expressed genes analysis,Gene Ontology(GO)enrichment analysis,KEGG pathway enrichment analysis,protein-protein interaction network analysis to screen out possible molecular mechanisms.ER-nucleus signaling pathway,cellular response to stress and apoptotic processes were identified the potential anti-cancer molecular biological process of GPM.GPM may also induce apoptosis in HepG2 cells via endoplasmic reticulum stress pathway.The mechanism is closely related to ERs,which might be beneficial for clinical therapy of HCC.CONCLUSION GPM can inhibit cells proliferation and induce apoptosis in HepG2 cells.The gene expression profile of GPM in HepG2 cells was obtained.The present study revealed the potential anti-tumor mechanism of GPM.展开更多
基金supported by the Fundamental Research Funds of Shandong University,China(No.2016JC016)
文摘In this work the effects of O_2 concentration on the pulsed dielectric barrier discharge in helium-oxygen mixture at atmospheric pressure have been numerically researched by using a one-dimensional fluid model in conjunction with the chosen key species and chemical reactions.The reliability of the used model has been examined by comparing the calculated discharge current with the reported experiments. The present work presents the following significant results. The dominative positive and negative particles are He_2~＋ and O_2^-, respectively, the densities of the reactive oxygen species（ROS） get their maxima nearly at the central position of the gap, and the density of the ground state O is highest in the ROS. The increase of O_2 concentration results in increasingly weak discharge and the time lag of the ignition. For O_2 concentrations below 1.1%,the density of O is much higher than other species, the averaged dissipated power density presents an evident increase for small O_2 concentration and then the increase becomes weak. In particular,the total density of the reactive oxygen species reaches its maximums at the O_2 concentration of about 0.5%. This characteristic further convinces the experimental observation that the O_2 concentration of 0.5% is an optimal O_2/He ratio in the inactivation of bacteria and biomolecules when radiated by using the plasmas produced in a helium oxygen mixture.
基金supported by Science and Technology Key Research Fund of Henan Province(142102310031)
文摘OBJECTIVE In order to investigate the possible anti-tumor molecular mechanisms of gecko polypeptide mixture(GPM).METHODS RNA-seq technology was used to identify the differentially expressed genes of human hepatocellular carcinoma(HCC)HepG2 cells treated with or without GPM.The HepG2 cells were treated with different concentration of GPM(0,0.1,0.2,0.3,0.4 mg·mL^(-1))for 6 h,12 h and 24 h,respectively.MTT assay was used to detect the viability of HepG2 cells.DAPI fluorescence staining was performed to observe nucleus morphological changes of HepG2 cells.Western blot analysis was applied to observe the expression of apoptosis-related proteins in HepG2 cells.RESULTS The results showed that GPM could induce HepG2 cells apoptosis and influence HepG2 cells proliferation in a dose-dependent manner.We applied many analysis methods,including differentially expressed genes analysis,Gene Ontology(GO)enrichment analysis,KEGG pathway enrichment analysis,protein-protein interaction network analysis to screen out possible molecular mechanisms.ER-nucleus signaling pathway,cellular response to stress and apoptotic processes were identified the potential anti-cancer molecular biological process of GPM.GPM may also induce apoptosis in HepG2 cells via endoplasmic reticulum stress pathway.The mechanism is closely related to ERs,which might be beneficial for clinical therapy of HCC.CONCLUSION GPM can inhibit cells proliferation and induce apoptosis in HepG2 cells.The gene expression profile of GPM in HepG2 cells was obtained.The present study revealed the potential anti-tumor mechanism of GPM.
