Hellebrigenin induces apoptosis in colorectal cancer Cells through induction of excessive reactive oxygen species

Traditional Chinese medicine(TCM)has been increasingly applied in both preventing and treating a variety of cancers in the last decades,attributing to its fewer side effects as compared with chemotherapy drugs.Hellebrigenin,a component of Chanpi from the skin of Bufo bufogargarizans Cantor or Duttaphrynus melanostictus has been reported to have an obvious anti-cancer activity on various cancers.However,the effect and mechanism of hellebrigenin on colorectal cancers were still unknown.Herein,the present study demonstrated hellebrigenin significantly reduced viability and triggered apoptosis via the intrinsic pathway in colorectal cancer cell lines HCT116 and HT29 in vitro and in vivo.Moreover,hellebrigenin led to a reduction of mitochondrial membrane potential.In addition,treatment with hellebrigenin could result in the induction of excessive reactive oxygen species,which led to cell apoptosis.These results indicated that hellebrigenin had anti-cancer potential in the treatment of colorectal cancers.

中华大蟾蜍皮的化学成分

目的研究中华大蟾蜍皮的化学成分。方法利用反复硅胶柱、ODS反相柱、制备液相、重结晶等手段分离分离纯化化学成分,根据化合物的理化性质和光谱数据并参考文献鉴定其化学结构。结果从中华大蟾蜍皮中分离得到6个化合物:胆甾醇(cholesterol,1)、棕榈酸胆甾烯酯(palmitatic acid cholesteryl ester,2)、蟾毒它灵(bufotalin,3)、沙蟾毒精(arenobufagin,4)、嚏根草配基(hellebrigenin,5)、嚏根草配基-3-辛二酸半酯(hellebrigenin-3-hemisuberate,6)。结论化合物6为新化合物。