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Electrophysiological functional recovery in a rat model of spinal cord hemisection injury following bone marrow-derived mesenchymal stem cell transplantation under hypothermia 被引量:8
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作者 Dong Wang Jianjun Zhang 《Neural Regeneration Research》 SCIE CAS CSCD 2012年第10期749-755,共7页
Following successful establishment of a rat model of spinal cord hemisection injury by resecting right spinal cord tissues, bone marrow stem cells were transplanted into the spinal cord lesions via the caudal vein whi... Following successful establishment of a rat model of spinal cord hemisection injury by resecting right spinal cord tissues, bone marrow stem cells were transplanted into the spinal cord lesions via the caudal vein while maintaining rectal temperature at 34 ± 0.5°C for 6 hours (mild hypothermia). Hematoxylin-eosin staining showed that astrocytes gathered around the injury site and formed scars at 4 weeks post-transplantation. Compared with rats transplanted with bone marrow stem cells under normal temperature, rats transplanted with bone marrow stem cells under hypothermia showed increased numbers of proliferating cells (bromodeoxyuridine-positive cells), better recovery of somatosensory-evoked and motor-evoked potentials, greater Basso, Beattie, and Bresnahan locomotor rating scores, and an increased degree of angle in the incline plate test. These findings suggested that hypothermia combined with bone marrow mesenchymal stem cells transplantation effectively promoted electrical conduction and nerve functional repair in a rat model of spinal cord hemisection injury. 展开更多
关键词 bone marrow mesenchymal stem cells electrophysiological function HYPOTHERMIA spinal cord hemisection injury TRANSPLANTATION
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Laminin-coated multifilament entubulation, combined with Schwann cells and glial cell line-derived neurotrophic factor, promotes unidirectional axonal regeneration in a rat model of thoracic spinal cord hemisection 被引量:5
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作者 Ling-Xiao Deng Nai-Kui Liu +3 位作者 Ryan Ning Wen Shuang-Ni Yang Xuejun Wen Xiao-Ming Xu 《Neural Regeneration Research》 SCIE CAS CSCD 2021年第1期186-191,共6页
Biomaterial bridging provides physical substrates to guide axonal growth across the lesion.To achieve efficient directional guidance,combinatory strategies using permissive matrix,cells and trophic factors are necessa... Biomaterial bridging provides physical substrates to guide axonal growth across the lesion.To achieve efficient directional guidance,combinatory strategies using permissive matrix,cells and trophic factors are necessary.In the present study,we evaluated permissive effect of poly(acrylonitrile-co-vinyl chloride)guidance channels filled by different densities of laminin-precoated unidirectional polypropylene filaments combined with Schwann cells,and glial cell line-derived neurotrophic factor for axonal regeneration through a T10 hemisected spinal cord gap in adult rats.We found that channels with filaments significantly reduced the lesion cavity,astrocytic gliosis,and inflammatory responses at the graft-host boundaries.The laminin coated low density filament provided the most favorable directional guidance for axonal regeneration which was enhanced by co-grafting of Schwann cells and glial cell line-derived neurotrophic factor.These results demonstrate that the combinatorial strategy of filament-filled guiding scaffold,adhesive molecular laminin,Schwann cells,and glial cell line-derived neurotrophic factor,provides optimal topographical cues in stimulating directional axonal regeneration following spinal cord injury.This study was approved by Indiana University Institutional Animal Care and Use Committees(IACUC#:11011)on October 29,2015. 展开更多
关键词 axonal regeneration extracellular molecule filament density hemisection LAMININ neurotrophic factor Schwann cell spinal cord injury THORACIC transplantation
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Different frequencies of electroacupuncture and semen coicis decrease glial fibrillary acidic protein expression in rats with hemisection spinal cord injury
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作者 Chunguang Hou Zhongzheng Li +4 位作者 Yi Guo Yongming Guo Yangyang Liu Chao Wang Ishida Torao 《Neural Regeneration Research》 SCIE CAS CSCD 2011年第24期1870-1875,共6页
This study established the following groups of rats: a normal group, a sham surgery group, a spinal cord injury model group, a low-frequency electroacupuncture group, a high-frequency electroacupuncture group and a s... This study established the following groups of rats: a normal group, a sham surgery group, a spinal cord injury model group, a low-frequency electroacupuncture group, a high-frequency electroacupuncture group and a semen coicis group. In all but the normal and sham surgery groups the left half of Tlo was transected. Four hours after model induction, 5-Hz and 100-Hz electroacupuncture were used to stimulate the acupoints I-luantiao (GB 30), Zusan/i (ST 36), Zhiyan9 (DU 9) and Xuanshu (DU 5), or crude extract from semen coicis was intraperitoneally injected, for 8 consecutive weeks. The results indicated that electroacupuncture stimulation and intraperitoneal injection of semen coicis improved the morphology of spinal cord tissue, promoted the recovery of motion-evoked potentials, suppressed glial fibrillary acidic protein expression, and ameliorated motor function in rats with hemisection spinal cord injury. The effects of high-frequency (100 Hz) electroacupuncture ancl semen coicis were significant. 展开更多
关键词 ELECTROACUPUNCTURE semen coicis hemisection spinal cord injury traditional Chinese medicine neural regeneration
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Coronal-Radicular Amputation or Hemisection? A Report Case in Lower Molar
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作者 Diana Araujo Espino Vicente Esparza Cesar Gaitan Fonseca 《Open Journal of Stomatology》 2016年第5期140-143,共4页
The coronal-radicular amputation or radicular hemisection is defined as the sectioning fragments coronal-radicular of the lower molar with clinical damage followed endodontic treatment and pros-thetics rehabilitation.... The coronal-radicular amputation or radicular hemisection is defined as the sectioning fragments coronal-radicular of the lower molar with clinical damage followed endodontic treatment and pros-thetics rehabilitation. This clinical treatment is viable in presence of the radicular decay or furca damage. This is a report case of radicular hemisection of lower molar with decay and bone loss that compromise distal root. The objective was elimination of distal root and conserved mesial root with endodontic and prosthetics treatment. 展开更多
关键词 Radicular hemisection Lower Molar Inferior Distal Root
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Biodegradable chitin conduit tubulation combined with bone marrow mesenchymal stem cell transplantation for treatment of spinal cord injury by reducing glial scar and cavity formation 被引量:7
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作者 Feng Xue Er-jun Wu +4 位作者 Pei-xun Zhang Li-ya A Yu-hui Kou Xiao-feng Yin Na Han 《Neural Regeneration Research》 SCIE CAS CSCD 2015年第1期104-111,共8页
We examined the restorative effect of modified biodegradable chitin conduits in combination with bone marrow mesenchymal stem cell transplantation after right spinal cord hemisection injury. Immunohistochemical staini... We examined the restorative effect of modified biodegradable chitin conduits in combination with bone marrow mesenchymal stem cell transplantation after right spinal cord hemisection injury. Immunohistochemical staining revealed that biological conduit sleeve bridging reduced glial scar formation and spinal muscular atrophy after spinal cord hemisection. Bone marrow mesenchymal stem cells survived and proliferated after transplantation in vivo, and differentiated into cells double-positive for S100 (Schwann cell marker) and glial fibrillary acidic protein (glial cell marker) at 8 weeks. Retrograde tracing showed that more nerve fibers had grown through the injured spinal cord at 14 weeks after combination therapy than either treatment alone. Our findings indicate that a biological conduit combined with bone marrow mesenchymal stem cell transplantation effectively prevented scar formation and provided a favorable local microenvi- ronment for the proliferation, migration and differentiation of bone marrow mesenchymal stem cells in the spinal cord, thus promoting restoration following spinal cord hemisection injury. 展开更多
关键词 nerve regeneration spinal cord injury spinal cord hemisection biological conduit bonemarrow mesenchymal stem cells stem cells transmission electron microscope cell transplantation neurons nerve fibers NSFC grants neural regeneration
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A comparative analysis of differentially expressed genes in rostral and caudal regions after spinal cord injury in rats
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作者 Xue-Min Cao Sheng-Long Li +4 位作者 Yu-Qi Cao Ye-Hua Lv Ya-Xian Wang Bin Yu Chun Yao 《Neural Regeneration Research》 SCIE CAS CSCD 2022年第10期2267-2271,共5页
The initial mechanical damage of a spinal cord injury(SCI)triggers a progressive secondary injury cascade,which is a complicated process integrating multiple systems and cells.It is crucial to explore the molecular an... The initial mechanical damage of a spinal cord injury(SCI)triggers a progressive secondary injury cascade,which is a complicated process integrating multiple systems and cells.It is crucial to explore the molecular and biological process alterations that occur after SCI for therapy development.The differences between the rostral and caudal regions around an SCI lesion have received little attention.Here,we analyzed the differentially expressed genes between rostral and caudal sites after injury to determine the biological processes in these two segments after SCI.We identified a set of differentially expressed genes,including Col3a1,Col1a1,Dcn,Fn1,Kcnk3,and Nrg1,between rostral and caudal regions at different time points following SCI.Functional enrichment analysis indicated that these genes were involved in response to mechanical stimulus,blood vessel development,and brain development.We then chose Col3a1,Col1a1,Dcn,Fn1,Kcnk3,and Nrg1 for quantitative real-time PCR and Fn1 for immunostaining validation.Our results indicate alterations in different biological events enriched in the rostral and caudal lesion areas,providing new insights into the pathology of SCI. 展开更多
关键词 biological process CAUDAL differentially expressed genes Gene Ontology hemisection IMMUNOSTAINING Rattus norvegicus RNA-sequencing rostral spinal cord injury
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