The traditional nutritional and medical hemp(Cannabis sativa L.)seed protein were explored for the discovery and directional preparation of new xanthine oxidase inhibitory(XOI)peptides by structure-based virtual scree...The traditional nutritional and medical hemp(Cannabis sativa L.)seed protein were explored for the discovery and directional preparation of new xanthine oxidase inhibitory(XOI)peptides by structure-based virtual screening,compound synthesis,in vitro bioassay and proteolysis.Six subtypes of hemp seed edestin and albumin were in silico hydrolyzed by 29 proteases,and 192 encrypted bioactive peptides were screened out.Six peptides showed to be XOI peptides,of which four(about 67%)were released by elastase hydrolysis.The peptide DDNPRRFY displayed the highest XOI activity(IC50=(2.10±0.06)mg/mL),acting as a mixed inhibitor.The pancreatic elastase directionally prepared XOI hemp seed protein hydrolysates,from which 6 high-abundance XOI peptides encrypted 3 virtually-screened ones including the DDNPRRFY.The novel outstanding hemp seed protein-derived XOI peptides and their virtual screening and directed preparation methods provide a promising and applicable approach to conveniently and efficiently explore food-derived bioactive peptides.展开更多
With changing dietary habits and increasing awareness of the nutraceutical role of dietary foods,the demand for natural plant proteins and interest in non-traditional protein sources in the food industry are increasin...With changing dietary habits and increasing awareness of the nutraceutical role of dietary foods,the demand for natural plant proteins and interest in non-traditional protein sources in the food industry are increasing.Industrial hemp,belonging to the plant family Cannabaceae,is cultivated for its fibre and edible seeds.Due to its nutritional value,it has also been used in the food industry and medicine.In particular,hemp seed proteins have drawn considerable attention in both scientific and industrial fields because of their excellent nutraceutical values,superior digestibility,low allergenicity and diverse techno-functional properties.In this review,we provide a summary of the current research progress on the extraction and purification processes,physiochemical properties,nutraceutical functions,and applications of hemp seed proteins.Perspectives in the application of advanced technologies for hemp seed bioactive peptide mining are also discussed.This review provides up-to-date insights into the nutraceutical values,health benefits,and future applications of this emerging plant source protein.展开更多
以火麻籽为原料,对碱提酸沉法提取火麻蛋白工艺参数进行研究,在单因素试验的基础上,采用响应面分析法对提取温度、提取时间、提取pH和料液比进行优化并得到回归模型。确定的碱提酸沉法提取火麻蛋白最佳工艺参数为:提取温度60℃,提取时间...以火麻籽为原料,对碱提酸沉法提取火麻蛋白工艺参数进行研究,在单因素试验的基础上,采用响应面分析法对提取温度、提取时间、提取pH和料液比进行优化并得到回归模型。确定的碱提酸沉法提取火麻蛋白最佳工艺参数为:提取温度60℃,提取时间1 h,提取pH 10,料液比1∶9。在最佳工艺条件下,火麻蛋白的提取率为63.5%。回归模型的预测值与实测值的相对误差为2.3%,该回归方程与实际情况拟合较好。DSC分析得出火麻蛋白的变性温度为83.0℃,纯化后的火麻蛋白相对分子质量分布均小于40 k Da。展开更多
采用碱提酸沉和经典Osborne分级分离的方法,分别制备得到了汉麻分离蛋白以及清蛋白、球蛋白、醇溶蛋白和谷蛋白,并研究了这5种分离蛋白的氨基酸组成、等电点、吸水吸油性、起泡性、乳化性、成膜性及凝胶性。结果表明:4种蛋白分离产物中...采用碱提酸沉和经典Osborne分级分离的方法,分别制备得到了汉麻分离蛋白以及清蛋白、球蛋白、醇溶蛋白和谷蛋白,并研究了这5种分离蛋白的氨基酸组成、等电点、吸水吸油性、起泡性、乳化性、成膜性及凝胶性。结果表明:4种蛋白分离产物中汉麻球蛋白的占比最高为46.80%,其次为清蛋白18.47%和谷蛋白18.00%,醇溶蛋白最少;汉麻球蛋白的多数氨基酸含量高于其他组分,并表现出了优异的氨基酸组成特性;5种分离蛋白的等电点依次为pH 4.5、p H 4.0、p H 5.0、p H 5.5和pH 5.5;汉麻分离蛋白起泡性最好,汉麻清蛋白的乳化性最好,汉麻分离蛋白和谷蛋白吸水性较强,清蛋白和球蛋白的吸油能力较强,5种分离蛋白在质量浓度50 mg/L下均可以揭膜,在200 mg/m L时汉麻分离蛋白、清蛋白和谷蛋白能够形成凝胶。展开更多
基金funded by National Natural Science Foundation of China(21868003)Bama County Program for Talents in Science and Technology(BaRenKe20210045).
文摘The traditional nutritional and medical hemp(Cannabis sativa L.)seed protein were explored for the discovery and directional preparation of new xanthine oxidase inhibitory(XOI)peptides by structure-based virtual screening,compound synthesis,in vitro bioassay and proteolysis.Six subtypes of hemp seed edestin and albumin were in silico hydrolyzed by 29 proteases,and 192 encrypted bioactive peptides were screened out.Six peptides showed to be XOI peptides,of which four(about 67%)were released by elastase hydrolysis.The peptide DDNPRRFY displayed the highest XOI activity(IC50=(2.10±0.06)mg/mL),acting as a mixed inhibitor.The pancreatic elastase directionally prepared XOI hemp seed protein hydrolysates,from which 6 high-abundance XOI peptides encrypted 3 virtually-screened ones including the DDNPRRFY.The novel outstanding hemp seed protein-derived XOI peptides and their virtual screening and directed preparation methods provide a promising and applicable approach to conveniently and efficiently explore food-derived bioactive peptides.
基金The financial support received from the Shenzhen Science and Technology Innovation Commission(KCXFZ20201221173207022)Youth Science Foundation Project(32101936)China Postdoctoral Science Foundation,No.15 Special Fund(In-Station)(2022T150366)。
文摘With changing dietary habits and increasing awareness of the nutraceutical role of dietary foods,the demand for natural plant proteins and interest in non-traditional protein sources in the food industry are increasing.Industrial hemp,belonging to the plant family Cannabaceae,is cultivated for its fibre and edible seeds.Due to its nutritional value,it has also been used in the food industry and medicine.In particular,hemp seed proteins have drawn considerable attention in both scientific and industrial fields because of their excellent nutraceutical values,superior digestibility,low allergenicity and diverse techno-functional properties.In this review,we provide a summary of the current research progress on the extraction and purification processes,physiochemical properties,nutraceutical functions,and applications of hemp seed proteins.Perspectives in the application of advanced technologies for hemp seed bioactive peptide mining are also discussed.This review provides up-to-date insights into the nutraceutical values,health benefits,and future applications of this emerging plant source protein.
文摘以火麻籽为原料,对碱提酸沉法提取火麻蛋白工艺参数进行研究,在单因素试验的基础上,采用响应面分析法对提取温度、提取时间、提取pH和料液比进行优化并得到回归模型。确定的碱提酸沉法提取火麻蛋白最佳工艺参数为:提取温度60℃,提取时间1 h,提取pH 10,料液比1∶9。在最佳工艺条件下,火麻蛋白的提取率为63.5%。回归模型的预测值与实测值的相对误差为2.3%,该回归方程与实际情况拟合较好。DSC分析得出火麻蛋白的变性温度为83.0℃,纯化后的火麻蛋白相对分子质量分布均小于40 k Da。
文摘采用碱提酸沉和经典Osborne分级分离的方法,分别制备得到了汉麻分离蛋白以及清蛋白、球蛋白、醇溶蛋白和谷蛋白,并研究了这5种分离蛋白的氨基酸组成、等电点、吸水吸油性、起泡性、乳化性、成膜性及凝胶性。结果表明:4种蛋白分离产物中汉麻球蛋白的占比最高为46.80%,其次为清蛋白18.47%和谷蛋白18.00%,醇溶蛋白最少;汉麻球蛋白的多数氨基酸含量高于其他组分,并表现出了优异的氨基酸组成特性;5种分离蛋白的等电点依次为pH 4.5、p H 4.0、p H 5.0、p H 5.5和pH 5.5;汉麻分离蛋白起泡性最好,汉麻清蛋白的乳化性最好,汉麻分离蛋白和谷蛋白吸水性较强,清蛋白和球蛋白的吸油能力较强,5种分离蛋白在质量浓度50 mg/L下均可以揭膜,在200 mg/m L时汉麻分离蛋白、清蛋白和谷蛋白能够形成凝胶。
