Objective:To elucidate the functional characterization of miR-218 in hepatocellualar carcinoma.Methods:miR-218 mimics and anti-miR-218 were transfected into HCC cells,and the cell proliferation and cell cycle were ana...Objective:To elucidate the functional characterization of miR-218 in hepatocellualar carcinoma.Methods:miR-218 mimics and anti-miR-218 were transfected into HCC cells,and the cell proliferation and cell cycle were analyzed by MTT assays and flow cytometry.Lv-miR-218 were constructed and miR-218 overexpression stable HCC cells were generated.The colony formation was assayed and the in vivo tumorigenesis was examined using xenograft tumor model in nude mouse.Results:miR-218 overexpression inhibited cell proliferation and induced cell cycle arrest in vitro,and in vivo study showed that miR-218 suppressed tumorigenesis in nude mouse.Conclusions:miR-218 may be a promising molecular target for HCC therapy.展开更多
目的:探讨肝特异性的miR-122表达在肝癌中的功能。方法:将miR-122类似物及反义序列分别转染HepG2细胞,Real time PCR检测成熟miR-122的表达量,MTT法检测miR-122对细胞增殖的影响,DAPI染色和流式细胞技术分析miR-122诱导的细胞凋亡情况...目的:探讨肝特异性的miR-122表达在肝癌中的功能。方法:将miR-122类似物及反义序列分别转染HepG2细胞,Real time PCR检测成熟miR-122的表达量,MTT法检测miR-122对细胞增殖的影响,DAPI染色和流式细胞技术分析miR-122诱导的细胞凋亡情况。结果:与对照组相比,miR-122转染后表达显著上调(P<0.01),明显抑制肝癌细胞的增殖,并且诱导促进肝癌细胞凋亡(P<0.01)。结论:miR-122参与对肝癌细胞的生长增殖的调控,有希望成为一个肝癌诊断及治疗的新靶标。展开更多
本研究探讨了美洲大蠊多肽PAE2逆转人肝细胞肿瘤耐药细胞株BEL-7402/5-FU的多药耐药性作用及机制。以人肝细胞肿瘤敏感细胞株BEL-7402、人肝细胞肿瘤耐5-氟尿嘧啶(5-FU)细胞株BEL-7402/5-FU和Balb/c-nude小鼠为研究对象,采用噻唑蓝比色...本研究探讨了美洲大蠊多肽PAE2逆转人肝细胞肿瘤耐药细胞株BEL-7402/5-FU的多药耐药性作用及机制。以人肝细胞肿瘤敏感细胞株BEL-7402、人肝细胞肿瘤耐5-氟尿嘧啶(5-FU)细胞株BEL-7402/5-FU和Balb/c-nude小鼠为研究对象,采用噻唑蓝比色分析法检测BEL-7402/5-FU细胞的多药耐药性和交叉耐药性,通过激光共聚焦显微镜观察阿霉素在BEL-7402/5-FU细胞中的累积。使用实时荧光定量聚合酶链式反应和免疫细胞化学染色法检测BEL-7402/5-FU细胞中P-糖蛋白1(P-glycoprotein 1,P-gp1)、多药耐药相关蛋白1(multi-drug resistance protein 1,MRP1)、肺耐药相关蛋白1(lung resistance protein 1,LRP1)以及乳腺癌耐药蛋白1(breast cancer resistance protein 1,BCRP1)的mRNA和蛋白的相对表达量,确定美洲大蠊多肽PAE2逆转多药耐药性的机制。此外,在Balb/c-nude小鼠中,建立BEL-7402/5-FU细胞的腋下移植瘤模型,观察美洲大蠊多肽PAE2对腋下移植瘤小鼠的免疫器官、肝肾功能、肿瘤抑制与诱导肿瘤细胞调亡、调节肿瘤组织多药耐药相关蛋白等环节的影响。结果显示,美洲大蠊多肽PAE2能有效逆转BEL-7402/5-FU细胞的多药耐药性和交叉耐药性,作用机制可能与下调多药耐药相关蛋白的表达水平有关,降低了多药耐药细胞对抗肿瘤药物的外排能力、影响抗肿瘤药物分子代谢,从而达到逆转人肝细胞癌多药耐药的作用。展开更多
基金Basic research project of Shenzhen science and technology innovation commission(No.JCYJ20160428171839409).
文摘Objective:To elucidate the functional characterization of miR-218 in hepatocellualar carcinoma.Methods:miR-218 mimics and anti-miR-218 were transfected into HCC cells,and the cell proliferation and cell cycle were analyzed by MTT assays and flow cytometry.Lv-miR-218 were constructed and miR-218 overexpression stable HCC cells were generated.The colony formation was assayed and the in vivo tumorigenesis was examined using xenograft tumor model in nude mouse.Results:miR-218 overexpression inhibited cell proliferation and induced cell cycle arrest in vitro,and in vivo study showed that miR-218 suppressed tumorigenesis in nude mouse.Conclusions:miR-218 may be a promising molecular target for HCC therapy.
文摘目的:探讨肝特异性的miR-122表达在肝癌中的功能。方法:将miR-122类似物及反义序列分别转染HepG2细胞,Real time PCR检测成熟miR-122的表达量,MTT法检测miR-122对细胞增殖的影响,DAPI染色和流式细胞技术分析miR-122诱导的细胞凋亡情况。结果:与对照组相比,miR-122转染后表达显著上调(P<0.01),明显抑制肝癌细胞的增殖,并且诱导促进肝癌细胞凋亡(P<0.01)。结论:miR-122参与对肝癌细胞的生长增殖的调控,有希望成为一个肝癌诊断及治疗的新靶标。
文摘本研究探讨了美洲大蠊多肽PAE2逆转人肝细胞肿瘤耐药细胞株BEL-7402/5-FU的多药耐药性作用及机制。以人肝细胞肿瘤敏感细胞株BEL-7402、人肝细胞肿瘤耐5-氟尿嘧啶(5-FU)细胞株BEL-7402/5-FU和Balb/c-nude小鼠为研究对象,采用噻唑蓝比色分析法检测BEL-7402/5-FU细胞的多药耐药性和交叉耐药性,通过激光共聚焦显微镜观察阿霉素在BEL-7402/5-FU细胞中的累积。使用实时荧光定量聚合酶链式反应和免疫细胞化学染色法检测BEL-7402/5-FU细胞中P-糖蛋白1(P-glycoprotein 1,P-gp1)、多药耐药相关蛋白1(multi-drug resistance protein 1,MRP1)、肺耐药相关蛋白1(lung resistance protein 1,LRP1)以及乳腺癌耐药蛋白1(breast cancer resistance protein 1,BCRP1)的mRNA和蛋白的相对表达量,确定美洲大蠊多肽PAE2逆转多药耐药性的机制。此外,在Balb/c-nude小鼠中,建立BEL-7402/5-FU细胞的腋下移植瘤模型,观察美洲大蠊多肽PAE2对腋下移植瘤小鼠的免疫器官、肝肾功能、肿瘤抑制与诱导肿瘤细胞调亡、调节肿瘤组织多药耐药相关蛋白等环节的影响。结果显示,美洲大蠊多肽PAE2能有效逆转BEL-7402/5-FU细胞的多药耐药性和交叉耐药性,作用机制可能与下调多药耐药相关蛋白的表达水平有关,降低了多药耐药细胞对抗肿瘤药物的外排能力、影响抗肿瘤药物分子代谢,从而达到逆转人肝细胞癌多药耐药的作用。
