Herb-partitioned moxibustion alleviates colon injuries in ulcerative colitis rats

AIM To observe the effect of herb-partitioned moxibustion(HPM) on expression of colonic cytokines in ulcerative colitis(UC) rats.METHODS A UC rat model was established by protein immunization in combination with topical chemical stimulation.Rats in the HPM group(n = 8) received HPM at bilateral Tianshu(ST25) points.The gross injury and pathological scores of the colon were recorded.The expression profile of colonic cytokines was assayed using the protein microarray technique.Specific differential cytokines were selected and verified by ELISA.The corresponding Uni Prot Accessions of the differentially expressed cytokines were retrieved in the Uni Prot database.The pathways involved were analyzed with the help of the KEGG PATHWAY database.The DAVID database was used for functional cluster and pathway analysis.RESULTS HPM improved colon injuries in UC rats,manifested by accelerated repair of ulcers and alleviation of inflammation,and the gross injury and pathological scores both significantly decreased(P < 0.01).Fold change > 1.3 or < 0.77 was taken as the screening standard.There were 77 down-regulated and 9 up-regulated differentially expressed colonic cytokines in the HPM group compared with the model group,and expression of 20 differed significantly(P < 0.05).Twelve of the 20 significantly differentially expressed cytokines [β-catenin,interleukin-1 receptor 6(IL-1 R6),IL-1β,B7-1,nerve growth factor receptor,AMP-activated protein kinase-α1,neuropilin-2,orexin A,adipocyte differentiation-related protein,IL-2,Fas and Fas L] were up-regulated in the model group(n = 3,compared with the normal group) but downregulated in the HPM group(n = 3,compared with the model group).Functional cluster analysis showed that the differentially expressed colonic cytokines in the HPM group regulated apoptosis and protein phosphorylation.KEGG pathway analysis showed that 52 down-regulated and 7 up-regulated differentially expressed colonic cytokines in the HPM group had pathways.The pathways that interacted between the cytokines and their receptors accounted for the largest proportion(28 of the downregulated and 5 of the up-regulated cytokines).CONCLUSION HPM promotes the repair of colon injuries in UC rats,which is related to the regulation of several abnormally expressed cytokines.

Extracellular signal-regulated kinase, substance P and neurokinin-1 are involved in the analgesic mechanism of herb-partitioned moxibustion

Herb-partitioned moxibustion can effectively mitigate visceral pain, a major symptom in inflammatory bowel disease, but the analgesic lnechanism is still unclear. Moreover, extracellular signal-regulated kinase, substance P, and neurokinin-1 are involved in formation of central hyperalgesia. Thus, we postulated that the analgesic effect of herb-partitioned moxibustion may be associated with these factors. Accordingly, in this study, we established an inflammatory bowel disease visceral pain model in rat by enema with a mixed solution of 5% trinitrobenzenesulfonic acid and 50% ethanol. Bilateral Tianshu （ST25） and Qihai （CV6） points were selected for herb-partitioned moxi- bustion. Our results showed that herb-partitioned moxibustion improved visceral pain and down-regulated extracellular signal-regulated kinase, substance P, and neurokinin-1 protein and mRNA expression in dorsal root ganglia. These results indicate that down-regulation of extracellular signal-regulated kinase, substance E and neurokinin-1 protein and mRNA may be a central mechanism for the analgesic effect of herb-partitioned moxibustion.

Moxibustion combined with acupuncture increases tight junction protein expression in Crohn's disease patients

AIM:To investigate the effect of herb-partitioned moxibustion combined with acupuncture on the expression of intestinal epithelial tight junction(TJ) proteins.METHODS:Sixty patients diagnosed with mild to moderate Crohn’s disease(CD)were allocated into the herb-partitioned moxibustion combined with acupuncture(HMA)group(n=30)or the mesalazine(MESA)group(n=30)using a parallel control method.There were 2 sets of acupoints used alternately for HMA treatment.The following points were included in Set A:ST25(Tianshu),RN6(Qihai),and RN9(Shuifen)for herb-partitioned moxibustion and ST36(Zusanli),ST37(Shangjuxu),LI11(Quchi),and LI4(Hegu)for acupuncture.The points for Set B included BL23(Shenshu)and BL25(Dachangshu)for herb-partitioned moxibustion and EX-B2 of T6-T1(Jiajixue)fo r acupuncture.The patients received the same treatment6 times a week for 12 consecutive weeks.The MESA group received 1 g of mesalazine enteric coated tablets4 times daily for 12 consecutive weeks.Intestinaltissues were stained and examined to compare the morphological and ultrastructural changes before and after the treatment session.Immunohistochemistry and in situ hybridization assays were used to detect the expression of intestinal epithelial TJ proteins zonula occludens-1(ZO-1),occludin,and claudin-1.The m RNA levels were also evaluated.RESULTS:After the treatment,both herb-partitioned moxibustion combined with acupuncture and mesalazine improved intestinal morphology and ultrastructure of CD patients;the patients treated with HMA showed better improvement.HMA significantly increased the expression of ZO-1(P=0.000),occludin(P=0.021),and claudin-1(P=0.016).MESA significantly increased the expression of ZO-1(P=0.016)and occludin(P=0.026).However,there was no significant increase in the expression of claudin-1(P=0.935).There was no statistically significant difference between the two groups for the expression of occludin and claudin-1(P>0.05).The HMA group showed a significant improvement in ZO-1 expression compared to the MESA group(2333.34±352.51 vs 2160.38±307.08,P=0.047).HMA significantly increased the expression of ZO-1 m RNA(P=0.000),occludin m RNA(P=0.017),and claudin-1 m RNA(P=0.017).MESA significantly increased the expression of ZO-1 m RNA(P=0.000),occludin m RNA(P=0.042),and claudin-1 m RNA(P=0.041).There was no statistically significant difference between the two groups in the expression of occludin and claudin-1 m RNA(P>0.05).However,the HMA group showed a significant improvement in ZO-1 m RNA expression compared with the MESA group(2378.17±308.77 vs 2200.56±281.88,P=0.023).CONCLUSION:HMA can repair intestinal epithelial barrier lesions and relieve inflammation by upregulating the expression of TJ proteins and their m RNAs.

Moxibustion eases chronic inflammatory visceral pain through regulating MEK, ERK and CREB in rats

AIM To investigate the effects of herb-partitioned moxibustion(HPM) on phosphorylation of mitogen-activated extracellular signal-regulated kinase(MEK)1, extracellular signal-regulated kinase(ERK)1/2 and c AMP response element binding protein(CREB) in spinal cord of rats with chronic inflammatory visceral pain(CIVP), and to explore the central mechanism of HPM in treating CIVP.METHODS Male Sprague-Dawley rats were randomized into normal, model, HPM, sham-HPM, MEK-inhibitor and dimethyl sulfoxide(DMSO) groups. The CIVP model was established using an enema mixture of trinitrobenzene sulfonic acid and ethanol. HPM was applied at bilateral Tianshu(ST25) and Qihai(CV6) acupoints in the HPM group, while in the sham-HPM group, moxa cones and herb cakes were only placed on the same points but not ignited. The MEK-inhibitor and DMSO groups received L5-L6 intrathecal injection of U0126 and 30% DMSO, respectively. Abdominal withdrawal reflex(AWR), mechanical withdrawal threshold(MWT) and thermal withdrawal latency(TWL) were applied for the assessment of pain behavior. The colonic tissue was observed under an optical microscope after hematoxylin-eosin staining. Expression of phosphor(p)MEK1, p ERK1/2 and p CREB in rat spinal cord was detected using Western blotting. The levels of MEK, ERK and CREB m RNA in rat spinal cord were detected using real-time polymerase chain reaction. RESULTS Compared with the normal group, the AWR scores were increased significantly(P < 0.01) and the MWT and TWL scores were decreased significantly(P < 0.05) in the model, sham-HPM and DMSO groups. Compared with the model group, the AWR scores were decreased significantly(P < 0.01) and the MWT and TWL scores were increased significantly in the HPM and MEK-inhibitor groups(P < 0.05). Compared with the sham-HPM and DMSO groups, the AWR scores were decreased significantly(P < 0.01) and the MWT and TWL scores were increased significantly(P < 0.05) in the HPM and MEK-inhibitor groups. Compared with the normal group, the expression of p MEK1, p ERK1/2 and p CREB proteins and the levels of MEK, ERK and CREB m RNA in rat spinal cord were increased significantly in the model, sham-HPM and DMSO groups(P < 0.01 or < 0.05). Compared with the model group, the expression of p MEK1, p ERK1/2 and p CREB proteins and the levels of MEK, ERK and CREB m RNA in rat spinal cord were reduced significantly in the HPM and MEK-inhibitor groups(P < 0.01 or < 0.05). Compared with the sham-HPM and DMSO groups, expression of p MEK1, p ERK1/2 and p CREB proteins and the levels of MEK, ERK and CREB m RNA in rat spinal cord were reduced significantly in the HPM and MEK-inhibitor groups(P < 0.01 or < 0.05). CONCLUSION HPM down-regulates protein phosphorylation of MEK1, ERK1/2 and CREB, and m RNA expression of MEK, ERK and CREB, inhibiting activation of the MEK/ERK/CREB signaling pathway in the spinal cord of CIVP rats, which is possibly a critical central mechanism of the analgesic effect of HPM.

Moxibustion inhibits interleukin-12 and tumor necrosis factor alpha and modulates intestinal flora in rat with ulcerative colitis

AIM: To investigate the effect of moxibustion on intestinal flora and release of interleukin-12 (IL-12) and tumor necrosis factor-α (TNF-α) from the colon in rat with ulcerative colitis (UC). METHODS: A rat model of UC was established by local stimulation of the intestine with supernatant from colonic contents harvested from human UC patients. A total of 40 male Sprague-Dawley rats were randomly divided into the following groups: normal (sham), model (UC), herb-partition moxibustion (HPM-treated), and positive control sulfasalazine (SA-treated). Rats treated with HPM received HPM at acupuncture points ST25 and RN6, once a day for 15 min, for a total of 8 d. Rats in the SA group were perfused with SA twice a day for 8 d. The colonic histopathology was observed by hematoxylin-eosin. The levels of intestinal flora, including Bifidobacterium, Lactobacillus, Escherichia coli (E. coli), and Bacteroides fragilis (B. fragilis), were tested by real-time quantitative polymerase chain reaction to detect bacterial 16S rRNA/DNA in order to determine DNA copy numbers of each specific species. Immunohistochemical assays were used to observe the expression of TNF-α and IL-12 in the rat colons. RESULTS: HPM treatment inhibited immunopathology in colonic tissues of UC rats; the general morphological score and the immunopathological score were significantly decreased in the HPM and SA groups compared with the model group [3.5 (2.0-4.0), 3.0 (1.5-3.5) vs 6.0 (5.5-7.0), P < 0.05 for the general morphological score, and 3.00 (2.00-3.50), 3.00 (2.50-3.50) vs 5.00 (4.50-5.50), P < 0.01 for the immunopathological score]. As measured by DNA copy number, we found that Bifidobacterium and Lactobacillus, which are associated with a healthy colon, were significantly higher in the HPM and SA groups than in the model group (1.395 ± 1.339, 1.461 ± 1.152 vs 0.045 ± 0.036, P < 0.01 for Bifidobacterium, and 0.395 ± 0.325, 0.851 ± 0.651 vs 0.0015 ± 0.0014, P < 0.01 for Lactobacillus). On the other hand, E. coli and B. fragilis, which are associated with an inflamed colon, were significantly lower in the HPM and SA groups than in the model group (0.244 ± 0.107, 0.628 ± 0.257 vs 1.691 ± 0.683, P < 0.01 for E. coli, and 0.351 ± 0.181, 0.416 ± 0.329 vs 1.285 ± 1.039, P < 0.01 for B. fragilis). The expression of TNF-α and IL-12 was decreased after HPM and SA treatment as compared to UC model alone (4970.81 ± 959.78, 6635.45 ± 1135.16 vs 12333.81 ± 680.79, P < 0.01 for TNF-α, and 5528.75 ± 1245.72, 7477.38 ± 1259.16 vs 12550.29 ± 1973.30, P < 0.01 for IL-12). CONCLUSION: HPM treatment can regulate intestinal flora and inhibit the expression of TNF-α and IL-12 in the colon tissues of UC rats, indicating that HPM can improve colonic immune response.

Effect of Herb-Partitioned Moxibustion on Structure and Functional Prediction of Gut Microbiota in Rats with Irritable Bowel Syndrome with Diarrhea

Objective:The objective of this study was to observe the effect of herb-partitioned moxibustion(HPM)on the gut microbiota of rats with diarrhea-predominant irritable bowel syndrome(IBS-D).Materials and Methods:A total of 48 male rats were randomly divided into a normal control group and an irritable bowel syndrome(IBS)model group.Using acetic acid irrigation and constraint stress,an IBS-D rat model was developed.After the model was made,the IBS rats were divided into IBS,HPM group,and pinaverium bromide(PB)group.The HPM received HPM for 20 min every day,while the PB was given gastric perfusion once a day for 14 days.After modeling and treatment,the abdominal withdrawal reflex,fecal character score,and fecal water content of rats were scored,and a 16S rRNA sequencing analysis was performed on the gut microbiota.Results:After treatment,the fecal character score and fecal water content in the HPM increased significantly,while visceral sensitivity decreased.Investigation of 16S rDNA sequencing revealed that α-diversity was reduced in the IBS,and HPM could increase the diversity of flora.The flora structure of IBS-D rats changed.HPM can increase the abundance of probiotics such as Akkermansia and reduce the abundance of opportunistic pathogens such as Bacteroides and Prevotella.Functional prediction analysis showed that the HPM was mainly related to the bacillary secret system,tricarboxylic acid cycle,and other pathways.Conclusion:HPM can regulate the gut microbiota of rats with IBS-D.

改良隔药脐灸联合敷贴序贯治疗小儿厌食症(脾气虚证)的临床观察

目的:探究与分析改良隔药脐灸联合敷贴序贯治疗小儿厌食症(脾气虚证)的临床疗效及对预后复发的影响。方法:选取我院2019年2月至2022年3月收治的小儿厌食症(脾气虚证)120例患儿,采取随机数字表法将其随机分为3组,每组各40例,对照组采用中药制剂厌食贴外敷治疗;治疗A组在对照组的基础上加用传统隔姜脐灸治疗;在对照组的基础上治疗B组加用改良隔药脐灸治疗,3组患儿均连续治疗4周,对比3组患儿的临床疗效、治疗前后主症积分和次症积分,同时对比3组治疗后4周、8周及12周的复发率。结果:治疗B组、治疗A组、对照组临床总有效率分别为95.00%(38/40)、80.00%(32/40)、62.5%(25/40),治疗B组临床总有效率均高于治疗A组和对照组(P<0.05)。3组患儿治疗后主症积分及次症积分均较治疗前降低,治疗B组患儿治疗后主症积分及次症积分均低于治疗A组、对照组,治疗A组患儿治疗后主症积分及次症积分均低于对照组,差异均有统计学意义(P<0.05)。随访8周时,治疗B组复发率[2.50%(1/40)]低于对照组[20.00%(8/40)],随访12周时,治疗B组复发率[2.50%(1/40)]低于治疗A组[17.50%(7/40)]和对照组[30.00%(12/40)],差异均有统计学意义(P<0.05)。结论:改良隔药脐灸与敷贴序贯联合治疗小儿厌食症(脾气虚证)可获得显著的临床疗效,促进改善患儿的临床症状及体征,同时在短期时间内有效避免了疾病的复发,具有较高的应用价值。

小儿推拿联合脐灸治疗慢性腹泻临床观察

目的分析小儿推拿与脐灸联合方案应用于慢性腹泻患儿中的临床效果及对其免疫功能产生的影响。方法以南昌市洪都中医院收治的满足纳入标准的60例慢性腹泻患儿为研究对象,采用随机数字表法分为对照组与观察组,每组30例。所有患儿均行常规基础治疗,对照组联合应用双歧杆菌三联活菌胶囊,观察组则予以小儿推拿与脐灸联合方案治疗,2周后比较两组的症状缓解时间、体征复常时间、免疫功能水平与临床效果。结果治疗后,观察组患儿的腹痛缓解、大便复常及便常规复常的时间均短于对照组(P<0.05);观察组患儿治疗后的IgA、IgG与CD^(8+)/CD^(4+)水平高于对照组(P<0.05);观察组患儿的总有效率是90.00%(27/30),高于对照组的73.33%(22/30)(P<0.05)。结论小儿推拿与脐灸联合方案应用于慢性腹泻患儿中的临床效果显著,有助于缩短患儿症状的缓解时间,增强机体免疫力。

穴位贴敷联合脐灸改善肺癌化疗患者癌因性疲乏的治疗效果

目的探究穴位贴敷联合脐灸改善肺癌化疗患者癌因性疲乏(CRF)的治疗效果。方法选取威海市中医院2022年7月—2023年1月收治的100例肺癌患者为受试对象,采用随机数字表法分为对照组与试验组,各50例。对照组给予中西医结合基础治疗,试验组在中西医结合基础治疗的同时采用穴位贴敷联合脐灸治疗。比较治疗前与治疗14 d后两组患者的中医证候评分、CRF情况[癌症疲乏量表(CFS)]、不良反应情况[MD Anderson症状评估(MDASITCM)]、生活质量情况(QLQ-LC43)评分。结果治疗14 d后,试验组总有效率(94.00%)高于对照组(78.00%,P<0.05);两组患者中医证候评分、CFS评分、MDASI-TCM评分及QLQ-LC43评分中的QLQ-C30、QLQ-LC13评分均较治疗前降低(P<0.01,P<0.05),且试验组均低于同一时间对照组(P<0.01,P<0.05)。结论穴位贴敷联合脐灸临床疗效显著,可有效缓解肺癌化疗患者CRF,减轻化疗不良反应,且有助于提高患者生活质量。

保和颗粒联合推拿及脐灸治疗小儿肠系膜淋巴结炎乳食积滞证的效果

目的探讨保和颗粒联合推拿及脐灸治疗小儿肠系膜淋巴结炎(ML)乳食积滞证的效果。方法选取2021年7月至2023年7月单县妇幼保健院收治的80例ML乳食积滞证患儿,按随机数字表法分为两组,每组各40例。对照组行推拿及脐灸治疗,观察组在对照组基础上采用保和颗粒治疗,比较两组临床疗效、中医证候积分、肠系膜淋巴结(MLNs)横径与纵径、不良反应发生情况。结果观察组治疗总有效率高于对照组(P<0.05);治疗后,观察组中医证候积分低于对照组(P<0.05),MLNs横径、纵径小于对照组(P<0.05);观察组腹痛、纳差、发热及恶心呕吐改善时间短于对照组(P<0.05);两组均无明显不良反应发生。结论保和颗粒联合推拿及脐灸治疗小儿ML乳食积滞证的效果较好,能够缩小MLNs横、纵径,降低中医证候积分。

Effect of herb-partitioned moxibustion on dopamine levels and dopamine receptor 1 expression in the colon and central nervous system in rats with Crohn’s disease

OBJECTIVE:To observe the effect of herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints in rats with Crohn's disease,and explore the underlying mechanism from dopamine (DA) and dopamine receptor 1 (D1 R) in the colon,spinal dorsal horn and hypothalamus.METHODS:The rats were randomly divided into the normal,model (CD),herb-partitioned moxibustion (Mox) and mesalazine (Mesa) groups.Damage in the colons was scored and observed by hematoxylin and eosin staining.DA and D1R protein expression in the colonic mucosa were detected by immunohistochemistry.The concentrations of DA and D1R in the spinal dorsal horn and hypothalamus were measured by enzyme-linked immunosorbent assay,and D1R mRNA expression was evaluated by quantitative real-time polymerase chain reaction.RESULTS:In the colon,compared with the normal group,DA,D1 R protein expressions and D1 R mRNA expression were significantly higher in the model group,while decreased in the Mox group and the Mesa group.In the spinal dorsal horn and hypothalamus,compared with the normal group,the concentrations of DA and D1 R,and the D1R mRNA expressions were significantly higher in the model group,and decreased in the Mox group and the Mesa group.CONCLUSION:Herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints relieved ulceration in CD rats,the underlying mechanism maybe relative with the regulation of DA and D1R in the colon,spinal dorsal horn and hypothalamus by moxibustion.

Herb-partitioned moxibustion regulated the miRNA expression profile in the thyroid tissues of rats with experimental autoimmune thyroiditis

OBJECTIVE:To observe the effect of herb-partitioned moxibustion(HPM)on the miRNA expression profile of thyroid tissue in experimental autoimmune thyroiditis(EAT)rats.METHODS:Rats were randomly divided into normal control(NC)group,EAT model(EAT)group,HPM group and western medicine(Med)group.EAT model rats were prepared by a combined immunization with complete and incomplete Freund’s adjuvant emulsified with porcine thyroglobulin and iodine.Rats in the HPM group were treated with HPM,while rats in the Med group were treated with levothyrocine(1μg/2 m L)by gavage.HE staining was used to observe the pathological morphological changes of thyroid tissue,ELISAs was uaed to detect the serum concentrations of TGAb,TPOAb,FT3,FT4,TSH.We then performed high-throughput mi RNA sequencing to analyse the mi RNA expression profiles in the thyroid tissues,followed by a bioinformatics analysis.RT-q PCR was used to verify the identified differentially expressed mi RNAs.RESULTS:HPM improved the thyroid tissue morphology and reduced serum TPOAb,TGAb,TSH concentration in EAT rats(P<0.05),but with no obvious effect on FT3 and FT4 concentration.While the TSH,FT3 and FT4 concentration was significantly changed in the Med group(P<0.01 or P<0.05)compared with that of EAT group.Sequencing results showed that a total of 17 mi RNAs were upregulated,and 4 were downregulated in the EAT rats,in which the expression levels of mi R-346 and mi R-331-5 p were reversed by HPM.The target genes of the mi RNAs that regulated by HPM wereassociated with a variety of immune factors and immune signals.RT-q PCR verification showed that the expression of mi RNA-346 and mi RNA-331-5 p was consistent with the sequencing results.CONCLUSIONS:HPM could regulate the the expression of mi RNA-346 and mi RNA-331-5 p,then act on their target genes to immune and inflammation-related pathways,which may be one of the mechanisms of HPM on EAT rats.

基于春夏养阳理论评价隔姜灸脐疗在慢性心力衰竭护理中的应用

[目的]观察隔姜灸脐疗对慢性心力衰竭患者临床症状及生活质量改善的效果。[方法]于2021年3月—2021年7月选择在中国中医科学院西苑医院心血管科住院治疗的心力衰竭患者80例,采用简单随机数字表法,分为观察组和对照组,每组各40例。对照组采用心力衰竭标准药物治疗及护理,观察组在常规治疗及护理的基础上辅以隔姜灸脐疗,每日3柱,14 d为1个疗程。比较两组心力衰竭患者心功能、血清N末端B型脑钠肽前体(NT-proBNP)水平、中医症状积分及明尼苏达心衰生活质量量表(MLHFQ)评分的改善情况。[结果]两组患者均完成研究,观察组心功能等级改善优于对照组,差异有统计学意义(P<0.05);两组患者中医证候积分均有所下降,且观察组优于对照组(P<0.05);两组患者干预后NT-proBNP水平均显著降低(P<0.05),与对照组比较,观察组治疗后血浆NT-proBNP水平明显降低(P<0.05);两组MLHFQ评分比较,与对照组比较,观察组治疗MLHFQ评分降低显著(P<0.05)。[结论]隔姜灸脐疗可有助于慢性心力衰竭患者的心功能及中医症状的改善,提高生活质量,操作简便易行,值得临床推广。

蔡圣朝教授重灸培阳治阳痿临床经验

阳痿是男科常见病,现代医学治疗本病存在药物副作用大、依赖性高等缺陷,而经数代医家孜孜探索,传统医学对阳痿治疗独具见解、颇具优势。全国名老中医蔡圣朝教授师承灸界泰斗周楣声的学术思想,认为阳痿的本质在于阳道不兴,而灸常不足,治疗上主张以温阳振痿为根本大法,提倡重灸培阳、扶阳培阴,振奋宗筋。

浮针疗法结合隔药灸脐治疗腹泻型肠易激综合征及对患者胃肠激素水平和菌群结构的影响

目的:观察浮针疗法结合隔药灸脐治疗腹泻型肠易激综合征及对患者胃肠激素和菌群结构水平的影响。方法:将92例已经确诊为肝脾不和证腹泻型肠易激综合征患者采用简单随机法分为西药组和中医组,各46例。西药组给予马来酸曲美布汀胶囊治疗,而中医组给予浮针疗法结合隔药灸脐治疗。比较两组中医症候积分、胃肠激素、菌群结构、IBS症状严重程度评分量表(IBS-SSS)评分、直肠感觉功能参数的差异,并统计两组疗效。结果:治疗前两组中医症候积分比较,差异无统计学意义(P>0.05),治疗后两组中医症候主症和次症积分较前下降,中医组治疗后中医症候积分低于西药组(P<0.05)。中医组总有效率为93.48%(43/46),明显高于西药组的78.26%(36/46),差异有统计学意义(P<0.05)。治疗前两组胃肠激素、菌群结构比较,差异无统计学意义(P>0.05),治疗后两组胃动素(MTL)、血管活性肠肽(VIP)、生长抑素(SS)及肠杆菌较前下降,双歧杆菌、乳杆菌及拟杆菌较治疗前升高,中医组治疗后胃肠激素、肠杆菌低于西药组,双歧杆菌、拟杆菌高于西药组(P<0.05)。治疗前两组IBS-SSS评分、直肠感觉功能参数比较,差异无统计学意义(P>0.05),治疗后两组初始感觉阈值、初始排便冲动阈值、最大耐受阈值较前升高,IBS-SSS评分较前下降,中医组治疗后直肠感觉功能参数高于西药组,IBS-SSS评分低于西药组(P<0.05)。结论:浮针疗法结合隔药灸脐治疗腹泻型肠易激综合征疗效较好,可有效降低内脏敏感性,改善临床症状,可能与调节胃肠激素和菌群结构有关。

温经汤联合脐灸治疗寒凝血瘀型原发性痛经的临床效果

目的:探讨温经汤联合脐灸治疗寒凝血瘀型原发性痛经的临床效果。方法:选取2021年5月—2022年8月于黔西市中医医院接受治疗的126例寒凝血瘀型原发性痛经患者为研究对象。根据随机数表法将其分为对照组和观察组,各63例。对照组给予西药治疗,观察组给予温经汤联合脐灸治疗。比较两组治疗前及治疗1个月经周期、2个月经周期、3个月经周期后的中医症候积分。比较两组治疗前、治疗3个月经周期后痛经情况、子宫动脉血流参数及不良反应。结果:治疗1个月经周期、2个月经周期、3个月经周期后,观察组中医症候积分低于对照组,差异有统计学意义(P<0.05)。治疗3个月经周期后,观察组视觉模拟评分法(VAS)、COX痛经症状量表(CMSS)评分均低于对照组,差异有统计学意义(P<0.05)。治疗3个月经周期后,观察组组搏动指数(PI)与阻力指数(RI)均低于对照组,差异有统计学意义(P<0.05)。观察组不良反应发生率低于对照组,差异有统计学意义(P<0.05)。结论:温经汤联合脐灸治疗寒凝血瘀型原发性痛经患者可有效缓解症状,减轻疼痛程度,调节子宫动脉血流动力学指标,安全性较高。

扶元固本方穴位贴敷联合脐灸对肺癌化疗患者癌因性疲乏症状、睡眠质量的影响

目的观察扶元固本方穴位贴敷联合脐灸对肺癌化疗患者癌因性疲乏症状、睡眠质量的影响,为改善肺癌化疗所致癌因性疲乏、睡眠质量差提供参考。方法选取2022年8月—2023年2月本院治疗的肺癌化疗患者86例为研究对象。按照随机数字表法分为对照组与观察组,每组43例。对照组进行常规治疗,观察组在对照组的基础上使用扶元固本方穴位贴敷联合脐灸进行治疗。持续治疗至患者化疗周期结束,比较两组癌因性疲乏评分、睡眠质量评分、生存质量评分。结果治疗后观察组临床总有效率高于对照组(P<0.01);观察组治疗后的癌因性疲乏评分显著低于对照组(P<0.01);观察组治疗后的睡眠质量评分显著低于对照组(P<0.01);观察组治疗后的生活质量各项评分显著高于对照组(P<0.01)。结论扶元固本方穴位贴敷联合脐灸在临床上有良好的疗效,能明显降低肺癌化疗患者癌因性疲乏评分,改善睡眠质量,同时提高患者的生活质量,值得临床上借鉴研究。

脐灸疗法治疗消化系统疾病的现状与探讨

目的分析目前脐灸疗法治疗消化系统疾病的文献研究,探讨脐灸治疗消化系统疾病的理论。方法在中国知网搜索了收录的篇名包含"脐灸"或"隔药灸脐"的文献,筛选出关于消化系统疾病的文献,进行分类处理,提炼出操作方法、治疗的病种、临床效应及作用机理。结果共发现脐灸治疗19种消化系统病症有效,其中隔药灸脐法应用最广,隔药灸脐还可以与针刺、中药内服、中药外敷、穴位贴敷、督灸、电针、西药内服等几种疗法的联合应用。结论脐灸治疗消化系统疾病的中医理论与神阙穴穴位特点、所选药物、灸法三者相关,现代理论机制尚未明确。

隔药灸脐对卵巢早衰模型大鼠Bcl-2、Bax蛋白和mRNA表达的影响

目的观察隔药灸脐对卵巢早衰模型大鼠Bcl-2和Bax表达的影响,探讨其对卵巢的保护机制。方法采用腹腔注射环磷酰胺建立大鼠卵巢早衰模型。48只雌性Wistar大鼠随机分为正常组、模型组、隔药灸脐组、西药组,每组12只。隔药灸脐组大鼠隔药灸"神阙",30 min/d,隔日1次,连续14 d;西药组大鼠予戊酸雌二醇灌胃,每日1次,连续14 d。采用免疫组化、Western blot和实时荧光定量PCR检测大鼠卵巢凋亡相关因子Bcl-2、Bax蛋白和mRNA的表达。结果模型组大鼠卵巢质量明显低于正常组(P<0.05)。Bcl-2、Bax蛋白主要表达在细胞胞浆中,隔药灸脐组和西药组较模型组卵泡颗粒细胞Bax表达范围明显缩小,Bcl-2表达范围明显增加。与正常组比较,模型组大鼠卵巢Bcl-2蛋白和mRNA表达明显降低(P<0.05,P<0.01),Bax蛋白和mRNA表达显著升高(P<0.05,P<0.01);与模型组比较,隔药灸脐组和西药组大鼠卵巢Bcl-2蛋白和mRNA表达显著升高(P<0.05,P<0.01),Bax蛋白和mRNA表达明显降低(P<0.05,P<0.01)。结论隔药灸脐可能通过上调卵巢早衰模型大鼠卵巢Bcl-2和下调Bax的表达,改善卵巢功能。

隔药灸脐法治疗肾虚血瘀型排卵障碍性不孕症患者的疗效观察

目的：以口服克罗米芬（CC）为对照,观察隔药灸脐法对肾虚血瘀型排卵障碍性不孕症患者的疗效。方法：采用双盲双模拟随机对照设计,将92例排卵障碍性不孕症患者分为治疗组和对照组,每组各46例。治疗组采用隔药灸脐法和口服安慰剂的治疗方法,对照组采用隔模拟剂灸脐法和口服CC的治疗方法,均治疗3个月经周期。观察妊娠率、排卵率、中医证候积分和总有效率。结果：两组妊娠率、排卵率差异无统计学意义（P〉0.05）,总有效率差异有统计学意义（P〈0.05）。两组中医证候积分均较治疗前明显降低（P〈0.05）,治疗后组间比较,差异具有统计学意义（P〈0.05,P〈0.01）。结论：治疗组能够促排助孕,明显改善中医证候积分,效果优于对照组。