The herbal drink “Attoté” has been widely used in the Abidjan district to treat a number of illnesses, notably erectile dysfunction. Despite the popularity of its therapeutic effects, very few studies have been...The herbal drink “Attoté” has been widely used in the Abidjan district to treat a number of illnesses, notably erectile dysfunction. Despite the popularity of its therapeutic effects, very few studies have been carried out on its effects on the health of users. The aim of this study was to identify the constituents contained in the phytomedicinal product and to assess their potential adverse effects in vivo. Phytochemical screening was conducted to identify the bioactive molecules in “Attoté” and to evaluate its hepatic effects in vivo. Forty (40) Wistar rats, randomly divided into 4 groups, with 10 animals per group (5 males and 5 females) were used to study potential hepatotoxic effects. Group 1 animals (control group) received distilled water. Batches I, II and III received by gavage a volume of Attoté extract corresponding to 1 ml/100 g body weight at 200 mg/kg, 400 mg/kg and 800 mg/kg, respectively. Attoté extract was administered daily at the same time for 28 days, and serum was collected every two weeks to assess hepatic biochemical markers by spectrophotometry using a Cobas C311® HITACHI biochemistry system. After one month of study, the rats were euthanized by ether overdose and the livers were harvested for morphological and histopathological analysis. Phytochemical screening revealed the presence of alkaloids, polyphenols, leucoanthocyanes, anthraquinones and quinones. Hepatic biochemical and hematological parameters such as red globular, hemoglobin, alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT), alkaline phosphatase (ALPs) and gamma glutamyl transferase (GGT) showed no significant change (p > 0.05) in the treated rat group compared with controls. However, these variations were moderate and transient, with values remaining almost within their standard limits. Microscopic observations of liver tissue sections from treated rats showed no liver damage or dysfunction. This study merits further investigation, with a view to gaining a better understanding of the cytotoxic mechanisms of herbal medicinal beverages, with a view to their reformulation as improved traditional medicines (ITMs).展开更多
建立了高效液相色谱快速测定草本植物饮料中28种外源性药物和内源性成分的分析方法。样品经甲醇-水(70∶30,v/v)超声提取后,使用Thermo Accucore C18色谱柱(100 mm×4.6 mm,2.6μm),以甲醇、乙腈和20m m o l/L乙酸铵溶液(p H 4.2)...建立了高效液相色谱快速测定草本植物饮料中28种外源性药物和内源性成分的分析方法。样品经甲醇-水(70∶30,v/v)超声提取后,使用Thermo Accucore C18色谱柱(100 mm×4.6 mm,2.6μm),以甲醇、乙腈和20m m o l/L乙酸铵溶液(p H 4.2)为流动相进行梯度洗脱,流速为1.2 m L/m in,柱温为35℃,采用二极管阵列检测器,检测波长为254和220 nm,外标法定量。结果表明,28种成分在1~100 mg/L范围内线性关系良好,相关系数(r)均不小于0.999。液体样品和固体样品的检出限(LODs)分别为1~10和20~200 mg/kg。28种成分在液体和固体样品中的加标回收率分别为88.8%~118.6%和92.7%~112.3%,相对标准偏差(RSDs)分别为0.1%~6.7%和0.1%~6.4%。按上述方法检测草本植物饮料456批,检出阳性样品55批次,检出率为12.1%。该法简便快速、准确可靠,适用于草本植物饮料中28种成分的测定。展开更多
文摘The herbal drink “Attoté” has been widely used in the Abidjan district to treat a number of illnesses, notably erectile dysfunction. Despite the popularity of its therapeutic effects, very few studies have been carried out on its effects on the health of users. The aim of this study was to identify the constituents contained in the phytomedicinal product and to assess their potential adverse effects in vivo. Phytochemical screening was conducted to identify the bioactive molecules in “Attoté” and to evaluate its hepatic effects in vivo. Forty (40) Wistar rats, randomly divided into 4 groups, with 10 animals per group (5 males and 5 females) were used to study potential hepatotoxic effects. Group 1 animals (control group) received distilled water. Batches I, II and III received by gavage a volume of Attoté extract corresponding to 1 ml/100 g body weight at 200 mg/kg, 400 mg/kg and 800 mg/kg, respectively. Attoté extract was administered daily at the same time for 28 days, and serum was collected every two weeks to assess hepatic biochemical markers by spectrophotometry using a Cobas C311® HITACHI biochemistry system. After one month of study, the rats were euthanized by ether overdose and the livers were harvested for morphological and histopathological analysis. Phytochemical screening revealed the presence of alkaloids, polyphenols, leucoanthocyanes, anthraquinones and quinones. Hepatic biochemical and hematological parameters such as red globular, hemoglobin, alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT), alkaline phosphatase (ALPs) and gamma glutamyl transferase (GGT) showed no significant change (p > 0.05) in the treated rat group compared with controls. However, these variations were moderate and transient, with values remaining almost within their standard limits. Microscopic observations of liver tissue sections from treated rats showed no liver damage or dysfunction. This study merits further investigation, with a view to gaining a better understanding of the cytotoxic mechanisms of herbal medicinal beverages, with a view to their reformulation as improved traditional medicines (ITMs).
文摘建立了高效液相色谱快速测定草本植物饮料中28种外源性药物和内源性成分的分析方法。样品经甲醇-水(70∶30,v/v)超声提取后,使用Thermo Accucore C18色谱柱(100 mm×4.6 mm,2.6μm),以甲醇、乙腈和20m m o l/L乙酸铵溶液(p H 4.2)为流动相进行梯度洗脱,流速为1.2 m L/m in,柱温为35℃,采用二极管阵列检测器,检测波长为254和220 nm,外标法定量。结果表明,28种成分在1~100 mg/L范围内线性关系良好,相关系数(r)均不小于0.999。液体样品和固体样品的检出限(LODs)分别为1~10和20~200 mg/kg。28种成分在液体和固体样品中的加标回收率分别为88.8%~118.6%和92.7%~112.3%,相对标准偏差(RSDs)分别为0.1%~6.7%和0.1%~6.4%。按上述方法检测草本植物饮料456批,检出阳性样品55批次,检出率为12.1%。该法简便快速、准确可靠,适用于草本植物饮料中28种成分的测定。
