人胃癌组织中herg1基因和HERG1蛋白过度表达的临床病理意义的研究

目的探讨herg1基因及其表达的HERG1蛋白在胃癌中的表达情况,研究其与胃癌间的相关性。方法应用免疫组化方法(SP法),RT-PCR技术及Western blot方法对64例胃癌组织标本及对应正常胃粘膜组织中的herg1基因及HERG1蛋白的表达进行检测,并进行回顾性随访。结果(1)RT-PCR分析胃癌组织中herg1基因mRNA阳性率为100%(64/64),正常组织中为0%(0/64)。(2)Western blot方法检测胃癌组织中HERG1蛋白表达阳性率为100%(64/64),正常组织中为0%(0/64)。(3)免疫组化分析HERG1蛋白在胃癌组织的阳性率为81.25%(52/64),与其病理类型无关,而在正常组织中未见明显表达,在伴有淋巴结转移或肝转移的胃癌herg1的阳性表达率为100%(10/10),无转移者为78%(42/54)。Hegr1基因的表达与胃癌的淋巴转移、肝转移有相关性(P<0.01)。结论Herg1基因和HERG1蛋白的过度表达参与了胃癌的发生发展过程,同时提示该基因和蛋白的检测可以预测胃癌组织的侵袭、转移倾向,为建立肿瘤转移的分子标志提供了理论和实验依据。

HERG K+ channels expression in gastric cancers and analysis of its regulation in tumor cell proliferation and apoptosis

Objective： To investigate the expression of hergl gene in tumor tissues from gastric carcinomas and gastric carcinoma cell lines, and study the relationship between HERG K＋ channel expressions and tumor cell proliferation and apoptosis. Methods： RT-PCR and PCR assays were used to detect the expression of hergl gene in 64 gastric carcinomas and the gastric cancer cell line SGC-7901. Blocking the HERG K＋ channels was used to evaluate their effects on tumor cell proliferation and apoptosis. Results：The statistically significant expression of hergl gene was detected in all the gastric cancers and SGC-7901 cells, but not in normal tissues. The HERG K＋ channel blocker, E-4031, increased the cell population in G0/G1（P 〈 0.05） and the number of apoptotic tumor cells（P 〈 0.05）. Conclusion： HERG K＋ channels were expressed in all gastric carcinomas tested and these channels appear to modulate tumor cell proliferation and apoptosis.