Chromatin is the primary carrier of epigenetic information in higher eukaryotes. AtCYP71 contains both cyclophilin domain and WD40 repeats. Loss of AtCYP71 function causes drastic pleiotropic phenotypic defects. Here,...Chromatin is the primary carrier of epigenetic information in higher eukaryotes. AtCYP71 contains both cyclophilin domain and WD40 repeats. Loss of AtCYP71 function causes drastic pleiotropic phenotypic defects. Here, we show that AtCYP71 physically interacts with FAS1 and LHP1, respectively, to modulate their distribution on chromatin. The Ihpl cyp71 double mutant showed more severe phenotypes than the single mutants, suggesting that AtCYP71 and LHP1 synergistically control plant development. Such synergism was in part illustrated by the observation that LHP1 association with its specific target loci requires AtCYP71 function. We also demonstrate that AtCYP71 physically interacts with FAS1 and is indispensable for FAS1 targeting to the KNAT1 locus. Together, our data suggest that AtCYP71 is involved in fundamental processes of chromatin assembly and histone modification in plants.展开更多
[目的]探讨异染色质蛋白1结合蛋白3(heterochromatin protein 1 binding protein 3,HP1BP3)在结直肠癌(colorectal cancer,CRC)中的表达及意义。[方法]通过外源性RNA干扰技术结合细胞增殖、侵袭和迁移体外实验探讨HP1BP3的肿瘤生物学效...[目的]探讨异染色质蛋白1结合蛋白3(heterochromatin protein 1 binding protein 3,HP1BP3)在结直肠癌(colorectal cancer,CRC)中的表达及意义。[方法]通过外源性RNA干扰技术结合细胞增殖、侵袭和迁移体外实验探讨HP1BP3的肿瘤生物学效应。免疫组织化学技术分析313例结直肠癌中HP1BP3的表达,并探讨其表达与患者临床病理特征的相关性。[结果]外源性敲低HP1BP3表达与SW480细胞增殖、侵袭和转移抑制相关。313例结直肠癌中,HP1BP3高表达104例,低表达209例,HP1BP3表达与患者年龄、性别、肿瘤原发部位及病理分期均无明显相关性(P均<0.05),与淋巴结转移情况密切相关(P=0.001)。[结论]HP1BP3低表达与结直肠肿瘤细胞发生发展抑制相关,其高表达与结直肠癌淋巴结转移相关,提示进展的结直肠癌中可能存在异常的染色质重塑。展开更多
文摘Chromatin is the primary carrier of epigenetic information in higher eukaryotes. AtCYP71 contains both cyclophilin domain and WD40 repeats. Loss of AtCYP71 function causes drastic pleiotropic phenotypic defects. Here, we show that AtCYP71 physically interacts with FAS1 and LHP1, respectively, to modulate their distribution on chromatin. The Ihpl cyp71 double mutant showed more severe phenotypes than the single mutants, suggesting that AtCYP71 and LHP1 synergistically control plant development. Such synergism was in part illustrated by the observation that LHP1 association with its specific target loci requires AtCYP71 function. We also demonstrate that AtCYP71 physically interacts with FAS1 and is indispensable for FAS1 targeting to the KNAT1 locus. Together, our data suggest that AtCYP71 is involved in fundamental processes of chromatin assembly and histone modification in plants.
文摘[目的]探讨异染色质蛋白1结合蛋白3(heterochromatin protein 1 binding protein 3,HP1BP3)在结直肠癌(colorectal cancer,CRC)中的表达及意义。[方法]通过外源性RNA干扰技术结合细胞增殖、侵袭和迁移体外实验探讨HP1BP3的肿瘤生物学效应。免疫组织化学技术分析313例结直肠癌中HP1BP3的表达,并探讨其表达与患者临床病理特征的相关性。[结果]外源性敲低HP1BP3表达与SW480细胞增殖、侵袭和转移抑制相关。313例结直肠癌中,HP1BP3高表达104例,低表达209例,HP1BP3表达与患者年龄、性别、肿瘤原发部位及病理分期均无明显相关性(P均<0.05),与淋巴结转移情况密切相关(P=0.001)。[结论]HP1BP3低表达与结直肠肿瘤细胞发生发展抑制相关,其高表达与结直肠癌淋巴结转移相关,提示进展的结直肠癌中可能存在异常的染色质重塑。
