A two-year field experiment conducted under dryland conditions in semi-humid and drought-prone regions of China aimed to assess the effect of ammonia-oxidizing bacterial on maize water use efficiency and yield.A heter...A two-year field experiment conducted under dryland conditions in semi-humid and drought-prone regions of China aimed to assess the effect of ammonia-oxidizing bacterial on maize water use efficiency and yield.A heterotrophic ammonia-oxidizing bacteria(HAOB)strain S2_8_1 was used.Six treatments were applied:(1)no irrigation+HAOB strain(DI),(2)no irrigation+blank culture medium(DM),(3)no irrigation control(DCK),(4)irrigation+HAOB(WI),(5)irrigation+blank culture medium(WM),and(6)irrigation control(WCK).Results revealed that HAOB treatment increased maize growth,yield,and water use efficiency over controls,regardless of whether the year was wet or dry.This improvement was attributed to the accelerated nitrification in the rhizosphere soil due to HAOB inoculation,which subsequently led to increased levels of leaf cytokinins.Overall,these findings suggest that HAOB inoculation holds promise as a strategy to boost water use efficiency and maize productivity in dryland agriculture.展开更多
Interest in the dynamics of soil respiration(R_(S))in subalpine forest ecosystems is increasing due to their high soil carbon density and potential sensitivity to environmental changes.However,as a principal silvicult...Interest in the dynamics of soil respiration(R_(S))in subalpine forest ecosystems is increasing due to their high soil carbon density and potential sensitivity to environmental changes.However,as a principal silvicultural practice,the long-term impacts of thinning on R_(S) and its heterotrophic and autotrophic respiration components(R_(h) and Ra,respectively)in subalpine plantations are poorly understood,espe-cially in winter.A 3-year field observation was carried out with consideration of winter CO_(2) efflux in middle-aged sub-alpine spruce plantations in northwestern China.A trench-ing method was used to explore the long-term impacts of thinning on Rs,Rn and R_(a).Seventeen years after thinning,mean annual Rs,Rn and R_(a) increased,while the contribu-tion of R_(h) to R_(s) decreased with thinning intensity.Thinning significantly decreased winter R,because of the reduction in R_(n) but had no significant effect on Ra.The temperature sensitivity(Q_(10))of R_(h) and R_(a) also increased with thinning intensity,with lower Q_(10) values for R_(h)(2.1-2.6)than for Ra(2.4-2.8).The results revealed the explanatory variables and pathways related to R_(n) and R_(a) dynamics.Thinning increased soil moisture and nitrate nitrogen(NO_(3)^(-)-N),and the enhanced nitrogen and water availability promoted R_(h) and R_(a) by improving fine root biomass and microbial activity.Our results highlight the positive roles of NO_(3)^(-)-N in stimulating R_(s) components following long-term thinning.Therefore,applications of nitrogen fertilizer are not recommended while thinning subalpine spruce plantations from the perspective of reducing soil CO_(2) emissions.The increased Q_(10) values of R_(s) components indicate that a large increase in soil CO_(2) emissions would be expected following thinning because of more pronounced climate warming in alpineregions.展开更多
Bacterium Providencia rettgeri YL was found to exhibit an unusual ability to heterotrophically nitrify and aerobically denitrify various concentrations of ammonium (NH4^+-N). In order to further understand its remo...Bacterium Providencia rettgeri YL was found to exhibit an unusual ability to heterotrophically nitrify and aerobically denitrify various concentrations of ammonium (NH4^+-N). In order to further understand its removal ability, several experiments were conducted to identify the growth and ammonium removal response at different carbon to nitrogen (C/N) mass ratios, shaking speeds, temperatures, ammonium concentrations and to qualitatively verify the production of nitrogen gas using gas chromatography techniques. Results showed that under optimum conditions (C/N 10, 30℃, 120 r/min), YL can significantly remove low and high concentrations of ammonium within 12 to 48 h of growth, respectively. The nitrification products hydroxylamine (NHzOH), nitrite (NO2^-) and nitrate (NO3^-) as well as the denitrification product, nitrogen gas (N2), were detected under completely aerobic conditions.展开更多
The effect of ultraviolet mutagenesis on a heterotrophic strain(Providencia JAT-1) mutation was studied and bioleaching of low grade copper ore with mutant bacteria was investigated. The results show that the activity...The effect of ultraviolet mutagenesis on a heterotrophic strain(Providencia JAT-1) mutation was studied and bioleaching of low grade copper ore with mutant bacteria was investigated. The results show that the activity of bacteria was improved after ultraviolet mutagenesis; the best irradiation time was 120 s. Compared to the original bacteria, the cells density of mutant bacteria at stationary phase increased by 26% and ammonia produced by mutant bacteria increased by 12%. Higher activity of bacteria leads to a higher copper extraction rate. The bioleaching performance of Providencia JAT-1 was improved after UV mutagenesis. The copper extraction rate with mutant bacteria increased by 10.6% compared to the original bacteria. The ore surface was corroded and the fine particles were absent after bioleaching. Free copper oxide and copper silicates could be leached out easily by using JAT-1; a small part of the copper sulfide can also be leached out. Bioleaching using JAT-1 is more effective than ammonia leaching and copper extraction rate with mutant bacteria was 21.1% higher than that by ammonia leaching under the same condition.展开更多
Objective To purify a low-temperature hydroxylamine oxidase (HAO) from a heterotrophic nitrifying bacterium Acinetobacter sp. Y26 and investigate the enzyme property. Methods A HAO was purified by an anion-exchange ...Objective To purify a low-temperature hydroxylamine oxidase (HAO) from a heterotrophic nitrifying bacterium Acinetobacter sp. Y26 and investigate the enzyme property. Methods A HAO was purified by an anion-exchange and gel-filtration chromatography from strain Y16. The purity and molecular mass were determined by RP-HPLC and SDS-PAGE. The HAO activity was detected by monitoring the reduction of potassium ferricyanide using hydroxylamine as substrate and ferricyanide as electron acceptor. The partial amino acid sequence was determined by mass spectrometry. Results The low-temperature HAO with a molecular mass of 61 kDa was purified from strain Y26 by an anion-exchange and gel-filtration chromatography. The enzyme exhibited an ability to oxidize hydroxylamine in wide temperature range (4-40 ℃) in vitro using hydroxylamine as substrate and ferricyanide as electron acceptor. It was stable in the temperature range of 4 to 25 ℃ and pH range of 6.0 to 8.5 with less than 30% change in its activity. The optimal temperature and pH were 15 ℃ and 7.5, respectively. Three peptides were determined by mass spectrometry which were shown to be not identical to other reported HAOs. Conclusion This is the first study to purify a low-temperature HAO from a heterotrophic nitrifier Acinetobecter sp. It differs from other reported HAOs in molecular mass and enzyme properties. The findings of the present study have suggested that the strain Y26 passes through a hydroxylamine-oxidizing process catalyzed by a low-temperature HAO for ammonium removal.展开更多
The study presented the method for isolating the heterotrophic nitrifiers and the characterization of heterotrophic nitrification. Continuous tests via a membrane bioreactor (MBR) were operated under the controlled co...The study presented the method for isolating the heterotrophic nitrifiers and the characterization of heterotrophic nitrification. Continuous tests via a membrane bioreactor (MBR) were operated under the controlled conditions to proliferate the nitrifiers. Heterotrophic nitrifying bacteria were isolated from the system in which the efficiency of total nitrogen(TN) removal was up to 80%. Since no autotrophic ammonium and nitrite oxidizers could be detected by fluorescence in situ hybridization(FISH), oxidized-N production was unlikely to be catalyzed by autotrophic nitrifiers during the heterotrophic nitrifiers' isolation in this study. The batch test results indicate that the isolated heterotrophic bacteria were able to nitrify. After 3 weeks incubation, the efficiencies of the COD removal by the three isolated bacterial strains B1, B2, and B3 were 52 6%, 71 7%, and 77 7%, respectively. The efficiencies of the TN removal by B1, B2, and B3 were 35 6%, 61 2% and 68 7%, respectively.展开更多
Nitrogen removal from synthetic wastewater was investigated in an airlift bioreactor (ALB), augmented with a novel heterotrophic nitrifier Pseudonocardia ammonioxydans H9^T under organic carbon to nitrogen ratios (...Nitrogen removal from synthetic wastewater was investigated in an airlift bioreactor (ALB), augmented with a novel heterotrophic nitrifier Pseudonocardia ammonioxydans H9^T under organic carbon to nitrogen ratios (Corg/N) ranging from 0 to 12. Effect of the inoculated strain was also determined on the settling properties and the removal of chemical oxygen demand (COD). Two laboratory scale reactors were set up to achieve a stable nitrifying state under the same physicochemical conditions of hydraulic retention time (HRT), temperature, pH and dissolved oxygen (DO), and operated under the sequencing batch mode. The level of DO was kept at 0.5- 1.5 mg/L by periodic stirring and aeration. Each specific Corg/N ratio was continued for duration of 3 weeks. One of the reactors (BR2) was inoculated with P ammonioxydans H9^T periodically at the start of each Corg/N ratio. Sludge volumetric index (SVI) improved with the increasing Corg/N ratio, but no significant difference was detected between the two reactors. BR2 showed higher levels of nitrogen removal with the increasing heterotrophic conditions, and the ammonia removal reached to the level of 82%-88%, up to10% higher than that in the control reactor (BR1) at Corg/N ratios higher than 6; however, the ammonia removal level in experimental reactor was up to 8% lower than that in control reactor at Corg/N ratios lower than 2. The COD removal efficiency progressively increased with the increasing Corg/N ratios in both of the reactors. The COD removal percentage up to peak values of 88%-94% in BR2, up to 11% higher than that in BR1 at Corg/N ratio higher than 4. The peak values of ammonia and COD removal almost coincided with the highest number (18%-27% to total bacterial number) of the exogenous bacterium in the BR2, detected as colony forming units (CFU). Furthermore, the removal of ammonia and COD in BR2 was closely related to the number of the inoculated strain with a coefficient index (R2) up to 0.82 and 0.85 for ammonia and COD, respectively. These results suggest that it was more efficient for both the ammonia and carbon nutrient removals in a reactor inoculated with a heterotrophic nitrifier at high Corg/N ratio, inferring that the heterotrophic nitrifers would be practically more available in the treatment of wastewater with high level of ammonia and COD.展开更多
The abundance of general aerobic heterotrophic bacteria(GAB) from the water and sediment in the Bering Sea and the Chukchi Sea was determined by using petri dish cultivation and counting method. The abundance of GAB a...The abundance of general aerobic heterotrophic bacteria(GAB) from the water and sediment in the Bering Sea and the Chukchi Sea was determined by using petri dish cultivation and counting method. The abundance of GAB among the different sea areas, sampling sites, layers of sediments surveyed and adaptability to differential temperatures was studied. The result obtained showed that: the occurrence percentage of GAB in the surface water was higher than that in sediment, but the abundance was only 0.17% of sediment. The occurrence percentage of GAB in surficial layer of sediment was higher than that in the other layers. The occurrence percentage of GAB in surficial layer of sediment was higher than that in the other layers. The occurrence percentage, abundance and its variation of GAB in the Bering Sea were higher than that in the Chukchi Sea respectively. The average value of the abundance in the all sediment surveyed was 3166.3×10 2 CFU·g -1(wet.). The abundance of GAB in sediment showed a trend: roughly higher in the lower latitudinal area than higher latitude. The results from temperature test mean that: the majority of bacteria tested were cold-adapted ones, minority might be mesophilic bacteria. The results indicated that, Arctic ocean bacteria had a stronger adaptability to environmental temperature.展开更多
The importance of dissolved organic phosphorus(DOP) as a potential nutrient source for primary producers in marine systems has been recognized for up to eight decades, but currently, the understanding of the biogeoche...The importance of dissolved organic phosphorus(DOP) as a potential nutrient source for primary producers in marine systems has been recognized for up to eight decades, but currently, the understanding of the biogeochemistry of DOP is in its infancy. In the present study, monthly data between 2000 and 2014 were used to analyze the temporal and spatial distributions of DOP in the Mir Bay, the northern South China Sea. The DOP residence time(TDOP) was also investigated using a simple regression analysis in combination with chlorophyll a(Chl a) measurements while excess DOP(ΔDOP), produced by the biogeochemical processes of autotrophic production and heterotrophic removal, was determined using a two-component mixing mass-balance model in combination with salinity measurements. The results showed that the DOP concentration was(0.017±0.010) mg/L higher in the surface-water compared with the bottom-water and higher in the inner Tolo Harbour and waters adjacent to Shatoujiao compared with the main zone of the bay. Although seasonal changes and annual variability in the DOP were small, the surface DOP concentration was higher in the wet season(April–September)than in the dry season(October–March) due to the impacts of seaward discharges and atmospheric deposition into the bay. Measurement and regression results showed that the DOP release rate from phytoplankton production was about 1.83(gP)/(gChl a) and the TDOP was about 7 d, which implied that the DOP cycle in the bay was rapid. The ΔDOP was calculated from the model to be about 0.000 mg/L in the main zone of the bay and about 0.002 mg/L in the inner Tolo Harbour and waters adjacent to Shaotoujiao, suggesting that the autotrophic production of DOP was almost balanced by the heterotrophic removal in the main zone of the bay and dominated in the inner Tolo Harbour and waters adjacent to Shaotoujiao. In conclusion, the Mirs Bay is very productive and fairly heterotrophic.展开更多
The seasonal abundance of flagellates has been monitored over a period of 1 year from December 2013 to November 2014(divided into 4 conjugative seasons namely winter,spring,summer,and autumn)in an experimental pond lo...The seasonal abundance of flagellates has been monitored over a period of 1 year from December 2013 to November 2014(divided into 4 conjugative seasons namely winter,spring,summer,and autumn)in an experimental pond located in Rajshahi City Corporation area,Bangladesh.To our knowledge,this study is the fi rst to shed light on the occurrence and possible interrelationships among heterotrophic flagellates(HF),bacteria and zooplankton in Bangladesh and the result obtained by this study will be beneficial for similar water ecosystem all over the world.Standard methods were used to determine the prescribed hydrological parameters and zooplankton cell density.Maximum HF abundance(14 346.00 cells/mL)was found in the spring and the minimum(5 215.00 cells/mL)occurred in the summer.Inverse to HF,significantly(P<0.05)higher zooplankton abundance was found during the winter(782.00±47.62 cells/mL)and the lowest value was found in the autumn(448.00±39.15 cells/mL).Whereas similar to the HF,total bacterial abundance was significantly higher during the spring((2.25±1.05)×10^5 cells/mL)and lower in the summer((0.79±0.06)×10^5 cells/mL).Multivariate analyses(ANOSIM and MDS)have shown significant seasonal differences for cell numbers where MDS ordination plot and cluster analysis based on similarity in the genera abundance of HF revealed overlapping condition between winter and spring.Canonical correspondence analysis(CCA)also showed a distinct separation among the genera based on the prevailing hydrological situation and indicated that temperature,pH,BOD5,and NO3^-were the most important environmental variables in determining the observed variation in HF community structure.Among the biological factors,zooplankton showed negative but total bacteria were positively correlated with HF abundance.展开更多
Nearly 20%–50% of the annual terrestrial dissolved organic carbon(DOC)from the Huanghe(Yellow)River was transported to the estuary during the 5-14d of water and sediment regulation.The concentration of DOC increased ...Nearly 20%–50% of the annual terrestrial dissolved organic carbon(DOC)from the Huanghe(Yellow)River was transported to the estuary during the 5-14d of water and sediment regulation.The concentration of DOC increased sharply during the period of water and sediment regulation,which may promote the terrestrial DOC consumption by heterotrophic bacterioplankton.Water and sediment regulation provides an ideal condition for the study of terrestrial DOC consumption by heterotrophic bacterioplankton when terrestrial DOC increases sharply in rainy season,which may help to seek the fates of terrestrial DOC in the estuaries and coasts.In this study,the concentration and stable isotope of DOC,the biomass,growth,and respiration of heterotrophic bacterioplankton were determined.By the study,we found both average percent contribution of terrestrial DOC to the DOC pool and Contribution of terrestrial DOC to the carbon composition of heterotrophic bacterioplankton decreased as distance from the river mouth increased off shore,which was deceased from(39.2±4.0)%,(37.5±4.3)%to(30.3±3.9)%,(28.2±3.9)%respectively.255-484μg C/(L·d)terrestrial DOC was consumed by heterotrophic bacterioplankton.And 29%-45%terrestrial DOC consumed by heterotrophic bacterioplankton releasing as CO2 by respiration.Comparing with tropical estuary,terrestrial DOC consumed by heterotrophic bacterioplankton was lower in temperate estuary(this study).Temperature may limit the consumption of terrestrial DOC by heterotrophic bacterioplankton.展开更多
The community composition,abundance and culturablity of heterotrophic bacterioplankton in the Dapeng Bay of the South China Sea were investigated by morphological method,bacterial cultivation and biochemical methods o...The community composition,abundance and culturablity of heterotrophic bacterioplankton in the Dapeng Bay of the South China Sea were investigated by morphological method,bacterial cultivation and biochemical methods over an annual cycle.Aeromonas and Pseudomonas were the predominant genera among the Gram-negative bacteria.The bacterioplankton abundance fluctuated from 2.07×10 8 to 2.44×10 9 cells/dm 3 ,and culturable bacteria abundance ranged between 0.1×10 6 and 16.07×10 6 CFU/dm 3 .The Culturablity of heterotrophic bacteria ranged between 0.01% and 1.31%.The relationship between bacterial culturablity and the environmental factors was also studied in different sites of the Dapeng Bay.Throughout the bay,a significantly positive relationship was evidenced between bacterial culturability and temperature,meanswhile bacterial culturability was significantly inversely related with salinity.The positive relationship was shown at the central bay while a negative relationship was observed at the bay mouth and there was no significant correlation at top bay between bacterial culturablity and chlorophyll a of sea water.A positive relationship was found between culturability and DIN all the bay,but DIP showed a significantly negative relationship with bacterial culturability only at the mouth of the bay.展开更多
Objective To characterize the heterotrophic nitrifying bacteria. Methods The bacteria were isolated from membrane bioreactor for treating synthetic wastewater using the method newly introduced in this study. Fluoresce...Objective To characterize the heterotrophic nitrifying bacteria. Methods The bacteria were isolated from membrane bioreactor for treating synthetic wastewater using the method newly introduced in this study. Fluorescence in situ hybridization (FISH) was used to validate the nonexistence of autotrophic ammonia oxidizers and nitrite oxidizers. Batch tests were carried out to investigate the capability of heterotrophic nitrification by the pure culture. Phylogenetic analysis of the pure culture was performed. Results A heterotrophic nitrifier, named Bacillus sp. LY, was newly isolated from the membrane bioreactor system in which the efficiency of TN removal was up to 80%. After 24-day, incubation, the removal efficiency of COD by Bacillus sp. LY was 71.7 %. The ammonium nitrogen removal rate after assimilation nearly ceased by Bacillus sp. LY was 74.7% The phylogenetic tree of Bacillus sp. LY and the neighbouring nitrifiers were given. Conclusions The batch test results indicate that Bacillus sp. LY can utilize the organic carbon as the source of assimilation when it grows on glucose and ammonium chloride medium accompanying the formation of oxidized-nitrogen. It also can denitrify nitrate while nitrifying. Bacillus sp. LY may become a new bacterial resource for heterotrophic nitrification and play a bioremediation role in nutrient removal.展开更多
An ammonia-oxidizing strain of Penicillium sp., M25-22 which was isolated from chicken feces compost, was studied to make clear its characteristics of ammonium oxidation and provide references for its application in a...An ammonia-oxidizing strain of Penicillium sp., M25-22 which was isolated from chicken feces compost, was studied to make clear its characteristics of ammonium oxidation and provide references for its application in aerobic composting. In media containing (NH4)2SO4 at a concentration of 0.5 g 100 mL^-1, mycelium weight and utilization ratio of ammonium nitrogen increased markedly during the 1st 5 d, concentration of nitrate increased markedly from the 3rd to the 5th d and concentration of nitrite remained at a low level. The strain showed the ability to oxidize ammonium nitrogen growing in media containing glucose, sucrose, starch, or cellulose as the sole carbon source and to oxidize the negative trivalent nitrogen of ammonium sulfate, peptone, acetamide, urea, or L-aspartate. Slowly available carbon or nitrogen sources such as starch, cellulose, and peptone were beneficial to ammonium oxidation. In media containing sucrose as the sole carbon source and ammonium sulfate as the sole nitrogen source, nitrate formation was the most pronounced with sucrose present at a concentration of 12 g L^-1 and ammonium nitrogen present at a concentration of 2.438 mg mL% at the initial pH level of 7.5 and at 30℃.展开更多
A strain capable of phenol degradation, heterotrophic nitrification and aerobic denitrification was isolated from activated sludge of coking-plant wastewater ponds under aerobic condition. Based on its morphology, phy...A strain capable of phenol degradation, heterotrophic nitrification and aerobic denitrification was isolated from activated sludge of coking-plant wastewater ponds under aerobic condition. Based on its morphology, physiology,biochemical analysis and phylogenetic characteristics, the isolate was identified as Diaphorobacter sp. PD-7.Biodegradation tests of phenol showed that the maximum phenol degradation occurred at the late phase of exponential growth stages, with 1400 mg·L-1phenol completely degraded within 85 h. Diaphorobacter sp. PD-7accumulated a vast quantity of phenol hydroxylase in this physiological phase, ensuring that the cells quickly utilize phenol as a sole carbon and energy source. The kinetic behavior of Diaphorobacter sp. PD-7 in batch cultures was investigated over a wide range of initial phenol concentrations(0–1400 mg·L-1) by using the Haldane model,which adequately describes the dynamic behavior of phenol biodegradation by strain Diaphorobacter sp. PD-7. At initial phenol concentration of 1400 mg·L-1, batch experiments(0.25 L flask) of nitrogen removal under aerobic condition gave almost entirely removal of 120.69 mg·L-1ammonium nitrogen within 75 h, while nitrate nitrogen removal reached 91% within 65 h. Moreover, hydroxylamine oxidase, periplasmic nitrate reductase and nitrite reductase were successfully expressed in the isolate.展开更多
-Marine chroococcoid phycoerythrin - containing Synechococcus spp. recently have been implicated as a substantial component of the photosynthetic picoplankton in the ocean. Although the importance of Synechococcus as ...-Marine chroococcoid phycoerythrin - containing Synechococcus spp. recently have been implicated as a substantial component of the photosynthetic picoplankton in the ocean. Although the importance of Synechococcus as food sources for heterotrophic nanoplankton are now recognized, the information about its cycling of biomass and diel patterns is limited and the methodology used varies according to different authors. A selective metabolic inhibitor method was used to allow simultanous estimation of both growth rates and grazing disappearance rates of Synechococcus. Results obtained in the English Channel show growth rates ranging from 0. 25 to 0. 72 d-1 with an average value of 0. 51 d -1and grazing disappearance rates ranged from 0. 21 to 0. 64 d-1 (mean = 0. 44 d-1). Offshore in the Celtic Sea of the Northeast Atlantic Ocean, both rates were lower than in the channel. The similarity between average growth and grazing rates suggests a rapid recycling of Synechococcus biomass. In diel pattern, Synechococcus grazing mortality rates were higher during the day (mean = 0. 61 d-1) than during the night (mean = 0. 21d-1) in all the experiments. A positive correlation was observed between growth rates and in situ temperature ranged from 9 to 20 ℃. Sire-fractionated experiments demonstrate that up to about 7 0 % of Synechococcus disappearance could be attributed to the grazer smaller than 2 μm in diameter. The variations of Synechococcus cell characteristics such as size and phycoerythin contents in the growth and grazing experiments were determined with a flow cytometer. The methodology recently used on estimating dynamics of Synechococcus population is reviewed.展开更多
Alcaligenes faecalis C16 was found to have the ability to heterotrophically nitrify and aerobically denitrify. In order to further understand its nitrogen removal ability and mechanism, the growth and ammonium removal...Alcaligenes faecalis C16 was found to have the ability to heterotrophically nitrify and aerobically denitrify. In order to further understand its nitrogen removal ability and mechanism, the growth and ammonium removal response were investigated at different C/N ratios and ammonium concentrations in the medium with citrate and acetate as carbon source separately. Furthermore, experiments of nitrogen sources, production of nitrogen gas and enzyme assay were conducted. Results show that the bacterium converts NH+4-N and produces NH2 OH during the growing phase and nitrite accumulation is its distinct metabolic feature. A. faecalis C16 is able to tolerate not only high ammonium concentration but also high C/N ratio, and the ammonium tolerance is associated with carbon source and C/N ratio. The nitrogen balance under different conditions shows that approximately28%–45% of the initial ammonium is assimilated into the cells, 44%–60% is denitrified and several percent is converted to nitrification products. A. faecalis C16 cannot utilize hydroxylamine, nitrite or nitrate as the sole nitrogen source for growth. However, nitrate can be used when ammonium is simultaneously present in the medium. A possible pathway for nitrogen removal by C16 is suggested. The preliminary enzyme assay provides more evidence for this nitrogen removal pathway.展开更多
The dust storms from the continent usually affect the abundance and diversity of planktons by supplying trace elements. As such, the response of heterotrophic planktonic bacteria to dusts, nutrients(i.e., nitrogen and...The dust storms from the continent usually affect the abundance and diversity of planktons by supplying trace elements. As such, the response of heterotrophic planktonic bacteria to dusts, nutrients(i.e., nitrogen and phosphorus) or ferrous dosages was investigated in the Kuroshio Extension region of the Northwest Pacific Ocean(NWPO) through on-board incubation experiments during an oceanographic survey in spring 2014. The flow cytometry and 16S rRNA high-throughput sequencing methods were applied to explore the abundance and community structure of bacteria, and the percentage of high nucleic acid bacteria(HNA%). The results showed that the heterotrophic bacteria abundance was low(average 2.55×10^5 cells mL^-1) and subjected to both nitrogen(N) and ferrous(Fe) limitation. Sand-dust deposition observably promoted the activity of heterotrophic planktonic bacteria. The maximum abundance of heterotrophic bacteria was 6.98×10^5 cells mL^-1 in the dust-dosage group, which was 44% higher than the control(P < 0.05). The HNA% in the dust-dosage group was 1.37 times higher than the control(P < 0.05). The activation mechanism was mainly related to the dissolution of N and Fe in the dusts. The relative abundance of genus Winogradskyella was significantly increased by dust deposition while the relative abundance of the genera Tenacibaculum and Hyphomonas was decreased. These variations of bacterial community structure were ascribed to the dissolution of nutrients N and P. Comparing the results of different experimental groups, this study concluded that dust storm improved the abundance of heterotrophic bacteria by dissolution of N and Fe.展开更多
From Oct., 1999 to Oct., 2000, the heterotrophic bacterial flora in the aquaculture area around Xuejiadao was investigated. The result shows that the populations of the heterotrophic bacteria are heavier in summer and...From Oct., 1999 to Oct., 2000, the heterotrophic bacterial flora in the aquaculture area around Xuejiadao was investigated. The result shows that the populations of the heterotrophic bacteria are heavier in summer and autumn than those in winter and spring. The average populations in seawater, sediment, the surface of seaweed and the surface of fish are 1.4×10 4 cfu mL -1, 5.4×10 6 cfu g -1, 1.5×10 6 cfu g -1 and 1.8×10 3 cfu cm -2, respectively. A total of 301 strains were isolated, among them 259 were Gram-negative. All the Gram-negative bacteria belong to 13 genera and some genera of Enterobacteriaceae. The communities of bacteria are slightly different among the samples. In the body surface of fish, Genus vibrio is dominant. In the remaining samples, dominant genus is Aeromonas.展开更多
基金supported by the National Natural Science Foundation of China(U1304326)the Natural Science Foundation of Henan Provincial(242300421242)。
文摘A two-year field experiment conducted under dryland conditions in semi-humid and drought-prone regions of China aimed to assess the effect of ammonia-oxidizing bacterial on maize water use efficiency and yield.A heterotrophic ammonia-oxidizing bacteria(HAOB)strain S2_8_1 was used.Six treatments were applied:(1)no irrigation+HAOB strain(DI),(2)no irrigation+blank culture medium(DM),(3)no irrigation control(DCK),(4)irrigation+HAOB(WI),(5)irrigation+blank culture medium(WM),and(6)irrigation control(WCK).Results revealed that HAOB treatment increased maize growth,yield,and water use efficiency over controls,regardless of whether the year was wet or dry.This improvement was attributed to the accelerated nitrification in the rhizosphere soil due to HAOB inoculation,which subsequently led to increased levels of leaf cytokinins.Overall,these findings suggest that HAOB inoculation holds promise as a strategy to boost water use efficiency and maize productivity in dryland agriculture.
基金supported by the National Natural Science Foundation of China (Grant Nos.41701296 and 42277481)the Natural Science Foundation of Gansu Province (GrantNo.22JR5RA058)the Youth Science and Technology Fund Program of Gansu Province (Grant No.22JR5RA087).
文摘Interest in the dynamics of soil respiration(R_(S))in subalpine forest ecosystems is increasing due to their high soil carbon density and potential sensitivity to environmental changes.However,as a principal silvicultural practice,the long-term impacts of thinning on R_(S) and its heterotrophic and autotrophic respiration components(R_(h) and Ra,respectively)in subalpine plantations are poorly understood,espe-cially in winter.A 3-year field observation was carried out with consideration of winter CO_(2) efflux in middle-aged sub-alpine spruce plantations in northwestern China.A trench-ing method was used to explore the long-term impacts of thinning on Rs,Rn and R_(a).Seventeen years after thinning,mean annual Rs,Rn and R_(a) increased,while the contribu-tion of R_(h) to R_(s) decreased with thinning intensity.Thinning significantly decreased winter R,because of the reduction in R_(n) but had no significant effect on Ra.The temperature sensitivity(Q_(10))of R_(h) and R_(a) also increased with thinning intensity,with lower Q_(10) values for R_(h)(2.1-2.6)than for Ra(2.4-2.8).The results revealed the explanatory variables and pathways related to R_(n) and R_(a) dynamics.Thinning increased soil moisture and nitrate nitrogen(NO_(3)^(-)-N),and the enhanced nitrogen and water availability promoted R_(h) and R_(a) by improving fine root biomass and microbial activity.Our results highlight the positive roles of NO_(3)^(-)-N in stimulating R_(s) components following long-term thinning.Therefore,applications of nitrogen fertilizer are not recommended while thinning subalpine spruce plantations from the perspective of reducing soil CO_(2) emissions.The increased Q_(10) values of R_(s) components indicate that a large increase in soil CO_(2) emissions would be expected following thinning because of more pronounced climate warming in alpineregions.
基金supported by the National Natural Science Foundation of China (No 20676078)
文摘Bacterium Providencia rettgeri YL was found to exhibit an unusual ability to heterotrophically nitrify and aerobically denitrify various concentrations of ammonium (NH4^+-N). In order to further understand its removal ability, several experiments were conducted to identify the growth and ammonium removal response at different carbon to nitrogen (C/N) mass ratios, shaking speeds, temperatures, ammonium concentrations and to qualitatively verify the production of nitrogen gas using gas chromatography techniques. Results showed that under optimum conditions (C/N 10, 30℃, 120 r/min), YL can significantly remove low and high concentrations of ammonium within 12 to 48 h of growth, respectively. The nitrification products hydroxylamine (NHzOH), nitrite (NO2^-) and nitrate (NO3^-) as well as the denitrification product, nitrogen gas (N2), were detected under completely aerobic conditions.
基金Project(2012BAB08B02)supported by the National Key Technologies R&D Program for the 12th Five-year Plan,ChinaProjects(51304011,51374035)supported by the National Natural Science Foundation of China
文摘The effect of ultraviolet mutagenesis on a heterotrophic strain(Providencia JAT-1) mutation was studied and bioleaching of low grade copper ore with mutant bacteria was investigated. The results show that the activity of bacteria was improved after ultraviolet mutagenesis; the best irradiation time was 120 s. Compared to the original bacteria, the cells density of mutant bacteria at stationary phase increased by 26% and ammonia produced by mutant bacteria increased by 12%. Higher activity of bacteria leads to a higher copper extraction rate. The bioleaching performance of Providencia JAT-1 was improved after UV mutagenesis. The copper extraction rate with mutant bacteria increased by 10.6% compared to the original bacteria. The ore surface was corroded and the fine particles were absent after bioleaching. Free copper oxide and copper silicates could be leached out easily by using JAT-1; a small part of the copper sulfide can also be leached out. Bioleaching using JAT-1 is more effective than ammonia leaching and copper extraction rate with mutant bacteria was 21.1% higher than that by ammonia leaching under the same condition.
基金supported by grants from National Natural Science Foundation of China(51078106)Heilongjiang Provincial Science Foundation for Distinguished Youth Scholar(JC200708)Heilongjiang Provincial Finance Foundation for Basic Sciences(CZ12BZSM06)
文摘Objective To purify a low-temperature hydroxylamine oxidase (HAO) from a heterotrophic nitrifying bacterium Acinetobacter sp. Y26 and investigate the enzyme property. Methods A HAO was purified by an anion-exchange and gel-filtration chromatography from strain Y16. The purity and molecular mass were determined by RP-HPLC and SDS-PAGE. The HAO activity was detected by monitoring the reduction of potassium ferricyanide using hydroxylamine as substrate and ferricyanide as electron acceptor. The partial amino acid sequence was determined by mass spectrometry. Results The low-temperature HAO with a molecular mass of 61 kDa was purified from strain Y26 by an anion-exchange and gel-filtration chromatography. The enzyme exhibited an ability to oxidize hydroxylamine in wide temperature range (4-40 ℃) in vitro using hydroxylamine as substrate and ferricyanide as electron acceptor. It was stable in the temperature range of 4 to 25 ℃ and pH range of 6.0 to 8.5 with less than 30% change in its activity. The optimal temperature and pH were 15 ℃ and 7.5, respectively. Three peptides were determined by mass spectrometry which were shown to be not identical to other reported HAOs. Conclusion This is the first study to purify a low-temperature HAO from a heterotrophic nitrifier Acinetobecter sp. It differs from other reported HAOs in molecular mass and enzyme properties. The findings of the present study have suggested that the strain Y26 passes through a hydroxylamine-oxidizing process catalyzed by a low-temperature HAO for ammonium removal.
文摘The study presented the method for isolating the heterotrophic nitrifiers and the characterization of heterotrophic nitrification. Continuous tests via a membrane bioreactor (MBR) were operated under the controlled conditions to proliferate the nitrifiers. Heterotrophic nitrifying bacteria were isolated from the system in which the efficiency of total nitrogen(TN) removal was up to 80%. Since no autotrophic ammonium and nitrite oxidizers could be detected by fluorescence in situ hybridization(FISH), oxidized-N production was unlikely to be catalyzed by autotrophic nitrifiers during the heterotrophic nitrifiers' isolation in this study. The batch test results indicate that the isolated heterotrophic bacteria were able to nitrify. After 3 weeks incubation, the efficiencies of the COD removal by the three isolated bacterial strains B1, B2, and B3 were 52 6%, 71 7%, and 77 7%, respectively. The efficiencies of the TN removal by B1, B2, and B3 were 35 6%, 61 2% and 68 7%, respectively.
基金This work was supported by the National Natural Science Foundation of China (No. 30470024).
文摘Nitrogen removal from synthetic wastewater was investigated in an airlift bioreactor (ALB), augmented with a novel heterotrophic nitrifier Pseudonocardia ammonioxydans H9^T under organic carbon to nitrogen ratios (Corg/N) ranging from 0 to 12. Effect of the inoculated strain was also determined on the settling properties and the removal of chemical oxygen demand (COD). Two laboratory scale reactors were set up to achieve a stable nitrifying state under the same physicochemical conditions of hydraulic retention time (HRT), temperature, pH and dissolved oxygen (DO), and operated under the sequencing batch mode. The level of DO was kept at 0.5- 1.5 mg/L by periodic stirring and aeration. Each specific Corg/N ratio was continued for duration of 3 weeks. One of the reactors (BR2) was inoculated with P ammonioxydans H9^T periodically at the start of each Corg/N ratio. Sludge volumetric index (SVI) improved with the increasing Corg/N ratio, but no significant difference was detected between the two reactors. BR2 showed higher levels of nitrogen removal with the increasing heterotrophic conditions, and the ammonia removal reached to the level of 82%-88%, up to10% higher than that in the control reactor (BR1) at Corg/N ratios higher than 6; however, the ammonia removal level in experimental reactor was up to 8% lower than that in control reactor at Corg/N ratios lower than 2. The COD removal efficiency progressively increased with the increasing Corg/N ratios in both of the reactors. The COD removal percentage up to peak values of 88%-94% in BR2, up to 11% higher than that in BR1 at Corg/N ratio higher than 4. The peak values of ammonia and COD removal almost coincided with the highest number (18%-27% to total bacterial number) of the exogenous bacterium in the BR2, detected as colony forming units (CFU). Furthermore, the removal of ammonia and COD in BR2 was closely related to the number of the inoculated strain with a coefficient index (R2) up to 0.82 and 0.85 for ammonia and COD, respectively. These results suggest that it was more efficient for both the ammonia and carbon nutrient removals in a reactor inoculated with a heterotrophic nitrifier at high Corg/N ratio, inferring that the heterotrophic nitrifers would be practically more available in the treatment of wastewater with high level of ammonia and COD.
文摘The abundance of general aerobic heterotrophic bacteria(GAB) from the water and sediment in the Bering Sea and the Chukchi Sea was determined by using petri dish cultivation and counting method. The abundance of GAB among the different sea areas, sampling sites, layers of sediments surveyed and adaptability to differential temperatures was studied. The result obtained showed that: the occurrence percentage of GAB in the surface water was higher than that in sediment, but the abundance was only 0.17% of sediment. The occurrence percentage of GAB in surficial layer of sediment was higher than that in the other layers. The occurrence percentage of GAB in surficial layer of sediment was higher than that in the other layers. The occurrence percentage, abundance and its variation of GAB in the Bering Sea were higher than that in the Chukchi Sea respectively. The average value of the abundance in the all sediment surveyed was 3166.3×10 2 CFU·g -1(wet.). The abundance of GAB in sediment showed a trend: roughly higher in the lower latitudinal area than higher latitude. The results from temperature test mean that: the majority of bacteria tested were cold-adapted ones, minority might be mesophilic bacteria. The results indicated that, Arctic ocean bacteria had a stronger adaptability to environmental temperature.
基金The National Key Basic Research Special Foundation of China under contract No.2013CB965101the Marine Science and Technology Foundation of the South China Sea Sub-administration,SOA,China under contract No.1624
文摘The importance of dissolved organic phosphorus(DOP) as a potential nutrient source for primary producers in marine systems has been recognized for up to eight decades, but currently, the understanding of the biogeochemistry of DOP is in its infancy. In the present study, monthly data between 2000 and 2014 were used to analyze the temporal and spatial distributions of DOP in the Mir Bay, the northern South China Sea. The DOP residence time(TDOP) was also investigated using a simple regression analysis in combination with chlorophyll a(Chl a) measurements while excess DOP(ΔDOP), produced by the biogeochemical processes of autotrophic production and heterotrophic removal, was determined using a two-component mixing mass-balance model in combination with salinity measurements. The results showed that the DOP concentration was(0.017±0.010) mg/L higher in the surface-water compared with the bottom-water and higher in the inner Tolo Harbour and waters adjacent to Shatoujiao compared with the main zone of the bay. Although seasonal changes and annual variability in the DOP were small, the surface DOP concentration was higher in the wet season(April–September)than in the dry season(October–March) due to the impacts of seaward discharges and atmospheric deposition into the bay. Measurement and regression results showed that the DOP release rate from phytoplankton production was about 1.83(gP)/(gChl a) and the TDOP was about 7 d, which implied that the DOP cycle in the bay was rapid. The ΔDOP was calculated from the model to be about 0.000 mg/L in the main zone of the bay and about 0.002 mg/L in the inner Tolo Harbour and waters adjacent to Shaotoujiao, suggesting that the autotrophic production of DOP was almost balanced by the heterotrophic removal in the main zone of the bay and dominated in the inner Tolo Harbour and waters adjacent to Shaotoujiao. In conclusion, the Mirs Bay is very productive and fairly heterotrophic.
文摘The seasonal abundance of flagellates has been monitored over a period of 1 year from December 2013 to November 2014(divided into 4 conjugative seasons namely winter,spring,summer,and autumn)in an experimental pond located in Rajshahi City Corporation area,Bangladesh.To our knowledge,this study is the fi rst to shed light on the occurrence and possible interrelationships among heterotrophic flagellates(HF),bacteria and zooplankton in Bangladesh and the result obtained by this study will be beneficial for similar water ecosystem all over the world.Standard methods were used to determine the prescribed hydrological parameters and zooplankton cell density.Maximum HF abundance(14 346.00 cells/mL)was found in the spring and the minimum(5 215.00 cells/mL)occurred in the summer.Inverse to HF,significantly(P<0.05)higher zooplankton abundance was found during the winter(782.00±47.62 cells/mL)and the lowest value was found in the autumn(448.00±39.15 cells/mL).Whereas similar to the HF,total bacterial abundance was significantly higher during the spring((2.25±1.05)×10^5 cells/mL)and lower in the summer((0.79±0.06)×10^5 cells/mL).Multivariate analyses(ANOSIM and MDS)have shown significant seasonal differences for cell numbers where MDS ordination plot and cluster analysis based on similarity in the genera abundance of HF revealed overlapping condition between winter and spring.Canonical correspondence analysis(CCA)also showed a distinct separation among the genera based on the prevailing hydrological situation and indicated that temperature,pH,BOD5,and NO3^-were the most important environmental variables in determining the observed variation in HF community structure.Among the biological factors,zooplankton showed negative but total bacteria were positively correlated with HF abundance.
基金Supported by the Guangdong Provincial Key Laboratory of Fishery Ecology and Environment(No.LFE-2015-7)the China Agriculture Research System(No.CARS-47-Z14)the Scientific and Technological Innovation Project of Shandong Marine and Fishery(No.2017HY10)
文摘Nearly 20%–50% of the annual terrestrial dissolved organic carbon(DOC)from the Huanghe(Yellow)River was transported to the estuary during the 5-14d of water and sediment regulation.The concentration of DOC increased sharply during the period of water and sediment regulation,which may promote the terrestrial DOC consumption by heterotrophic bacterioplankton.Water and sediment regulation provides an ideal condition for the study of terrestrial DOC consumption by heterotrophic bacterioplankton when terrestrial DOC increases sharply in rainy season,which may help to seek the fates of terrestrial DOC in the estuaries and coasts.In this study,the concentration and stable isotope of DOC,the biomass,growth,and respiration of heterotrophic bacterioplankton were determined.By the study,we found both average percent contribution of terrestrial DOC to the DOC pool and Contribution of terrestrial DOC to the carbon composition of heterotrophic bacterioplankton decreased as distance from the river mouth increased off shore,which was deceased from(39.2±4.0)%,(37.5±4.3)%to(30.3±3.9)%,(28.2±3.9)%respectively.255-484μg C/(L·d)terrestrial DOC was consumed by heterotrophic bacterioplankton.And 29%-45%terrestrial DOC consumed by heterotrophic bacterioplankton releasing as CO2 by respiration.Comparing with tropical estuary,terrestrial DOC consumed by heterotrophic bacterioplankton was lower in temperate estuary(this study).Temperature may limit the consumption of terrestrial DOC by heterotrophic bacterioplankton.
基金The National Natural Science Foundation of China under contract Nos 30770340 and 30470281the National Major Program of Science and Technology for Water Pollution Control and Restoration in China under contract Nos 2009ZX07423-003 and 2009ZX07101-011Shenzhen Grant Plan for Science and Technology
文摘The community composition,abundance and culturablity of heterotrophic bacterioplankton in the Dapeng Bay of the South China Sea were investigated by morphological method,bacterial cultivation and biochemical methods over an annual cycle.Aeromonas and Pseudomonas were the predominant genera among the Gram-negative bacteria.The bacterioplankton abundance fluctuated from 2.07×10 8 to 2.44×10 9 cells/dm 3 ,and culturable bacteria abundance ranged between 0.1×10 6 and 16.07×10 6 CFU/dm 3 .The Culturablity of heterotrophic bacteria ranged between 0.01% and 1.31%.The relationship between bacterial culturablity and the environmental factors was also studied in different sites of the Dapeng Bay.Throughout the bay,a significantly positive relationship was evidenced between bacterial culturability and temperature,meanswhile bacterial culturability was significantly inversely related with salinity.The positive relationship was shown at the central bay while a negative relationship was observed at the bay mouth and there was no significant correlation at top bay between bacterial culturablity and chlorophyll a of sea water.A positive relationship was found between culturability and DIN all the bay,but DIP showed a significantly negative relationship with bacterial culturability only at the mouth of the bay.
基金supported by the National Natural Science Foundation of China (No. 20176027).
文摘Objective To characterize the heterotrophic nitrifying bacteria. Methods The bacteria were isolated from membrane bioreactor for treating synthetic wastewater using the method newly introduced in this study. Fluorescence in situ hybridization (FISH) was used to validate the nonexistence of autotrophic ammonia oxidizers and nitrite oxidizers. Batch tests were carried out to investigate the capability of heterotrophic nitrification by the pure culture. Phylogenetic analysis of the pure culture was performed. Results A heterotrophic nitrifier, named Bacillus sp. LY, was newly isolated from the membrane bioreactor system in which the efficiency of TN removal was up to 80%. After 24-day, incubation, the removal efficiency of COD by Bacillus sp. LY was 71.7 %. The ammonium nitrogen removal rate after assimilation nearly ceased by Bacillus sp. LY was 74.7% The phylogenetic tree of Bacillus sp. LY and the neighbouring nitrifiers were given. Conclusions The batch test results indicate that Bacillus sp. LY can utilize the organic carbon as the source of assimilation when it grows on glucose and ammonium chloride medium accompanying the formation of oxidized-nitrogen. It also can denitrify nitrate while nitrifying. Bacillus sp. LY may become a new bacterial resource for heterotrophic nitrification and play a bioremediation role in nutrient removal.
基金study was supported by the National Natural Science Foundation of China (NSFC-30371054)
文摘An ammonia-oxidizing strain of Penicillium sp., M25-22 which was isolated from chicken feces compost, was studied to make clear its characteristics of ammonium oxidation and provide references for its application in aerobic composting. In media containing (NH4)2SO4 at a concentration of 0.5 g 100 mL^-1, mycelium weight and utilization ratio of ammonium nitrogen increased markedly during the 1st 5 d, concentration of nitrate increased markedly from the 3rd to the 5th d and concentration of nitrite remained at a low level. The strain showed the ability to oxidize ammonium nitrogen growing in media containing glucose, sucrose, starch, or cellulose as the sole carbon source and to oxidize the negative trivalent nitrogen of ammonium sulfate, peptone, acetamide, urea, or L-aspartate. Slowly available carbon or nitrogen sources such as starch, cellulose, and peptone were beneficial to ammonium oxidation. In media containing sucrose as the sole carbon source and ammonium sulfate as the sole nitrogen source, nitrate formation was the most pronounced with sucrose present at a concentration of 12 g L^-1 and ammonium nitrogen present at a concentration of 2.438 mg mL% at the initial pH level of 7.5 and at 30℃.
基金the National Natural Science Foundation of China(51378330 and51408396)the Natural Science Foundation of Shanxi Province(2013021023-3)
文摘A strain capable of phenol degradation, heterotrophic nitrification and aerobic denitrification was isolated from activated sludge of coking-plant wastewater ponds under aerobic condition. Based on its morphology, physiology,biochemical analysis and phylogenetic characteristics, the isolate was identified as Diaphorobacter sp. PD-7.Biodegradation tests of phenol showed that the maximum phenol degradation occurred at the late phase of exponential growth stages, with 1400 mg·L-1phenol completely degraded within 85 h. Diaphorobacter sp. PD-7accumulated a vast quantity of phenol hydroxylase in this physiological phase, ensuring that the cells quickly utilize phenol as a sole carbon and energy source. The kinetic behavior of Diaphorobacter sp. PD-7 in batch cultures was investigated over a wide range of initial phenol concentrations(0–1400 mg·L-1) by using the Haldane model,which adequately describes the dynamic behavior of phenol biodegradation by strain Diaphorobacter sp. PD-7. At initial phenol concentration of 1400 mg·L-1, batch experiments(0.25 L flask) of nitrogen removal under aerobic condition gave almost entirely removal of 120.69 mg·L-1ammonium nitrogen within 75 h, while nitrate nitrogen removal reached 91% within 65 h. Moreover, hydroxylamine oxidase, periplasmic nitrate reductase and nitrite reductase were successfully expressed in the isolate.
文摘-Marine chroococcoid phycoerythrin - containing Synechococcus spp. recently have been implicated as a substantial component of the photosynthetic picoplankton in the ocean. Although the importance of Synechococcus as food sources for heterotrophic nanoplankton are now recognized, the information about its cycling of biomass and diel patterns is limited and the methodology used varies according to different authors. A selective metabolic inhibitor method was used to allow simultanous estimation of both growth rates and grazing disappearance rates of Synechococcus. Results obtained in the English Channel show growth rates ranging from 0. 25 to 0. 72 d-1 with an average value of 0. 51 d -1and grazing disappearance rates ranged from 0. 21 to 0. 64 d-1 (mean = 0. 44 d-1). Offshore in the Celtic Sea of the Northeast Atlantic Ocean, both rates were lower than in the channel. The similarity between average growth and grazing rates suggests a rapid recycling of Synechococcus biomass. In diel pattern, Synechococcus grazing mortality rates were higher during the day (mean = 0. 61 d-1) than during the night (mean = 0. 21d-1) in all the experiments. A positive correlation was observed between growth rates and in situ temperature ranged from 9 to 20 ℃. Sire-fractionated experiments demonstrate that up to about 7 0 % of Synechococcus disappearance could be attributed to the grazer smaller than 2 μm in diameter. The variations of Synechococcus cell characteristics such as size and phycoerythin contents in the growth and grazing experiments were determined with a flow cytometer. The methodology recently used on estimating dynamics of Synechococcus population is reviewed.
基金National Natural Science Foundation of China(51078252)the Science and Technology Program of Taiyuan(120233)the Natural Science Foundation of Shanxi Province(2010011016–1)
文摘Alcaligenes faecalis C16 was found to have the ability to heterotrophically nitrify and aerobically denitrify. In order to further understand its nitrogen removal ability and mechanism, the growth and ammonium removal response were investigated at different C/N ratios and ammonium concentrations in the medium with citrate and acetate as carbon source separately. Furthermore, experiments of nitrogen sources, production of nitrogen gas and enzyme assay were conducted. Results show that the bacterium converts NH+4-N and produces NH2 OH during the growing phase and nitrite accumulation is its distinct metabolic feature. A. faecalis C16 is able to tolerate not only high ammonium concentration but also high C/N ratio, and the ammonium tolerance is associated with carbon source and C/N ratio. The nitrogen balance under different conditions shows that approximately28%–45% of the initial ammonium is assimilated into the cells, 44%–60% is denitrified and several percent is converted to nitrification products. A. faecalis C16 cannot utilize hydroxylamine, nitrite or nitrate as the sole nitrogen source for growth. However, nitrate can be used when ammonium is simultaneously present in the medium. A possible pathway for nitrogen removal by C16 is suggested. The preliminary enzyme assay provides more evidence for this nitrogen removal pathway.
基金funded by the National Natural Science Foundation of China (No.41210008)the Major State Basic Research Development Program of China (973 Program No.2014CB953701)。
文摘The dust storms from the continent usually affect the abundance and diversity of planktons by supplying trace elements. As such, the response of heterotrophic planktonic bacteria to dusts, nutrients(i.e., nitrogen and phosphorus) or ferrous dosages was investigated in the Kuroshio Extension region of the Northwest Pacific Ocean(NWPO) through on-board incubation experiments during an oceanographic survey in spring 2014. The flow cytometry and 16S rRNA high-throughput sequencing methods were applied to explore the abundance and community structure of bacteria, and the percentage of high nucleic acid bacteria(HNA%). The results showed that the heterotrophic bacteria abundance was low(average 2.55×10^5 cells mL^-1) and subjected to both nitrogen(N) and ferrous(Fe) limitation. Sand-dust deposition observably promoted the activity of heterotrophic planktonic bacteria. The maximum abundance of heterotrophic bacteria was 6.98×10^5 cells mL^-1 in the dust-dosage group, which was 44% higher than the control(P < 0.05). The HNA% in the dust-dosage group was 1.37 times higher than the control(P < 0.05). The activation mechanism was mainly related to the dissolution of N and Fe in the dusts. The relative abundance of genus Winogradskyella was significantly increased by dust deposition while the relative abundance of the genera Tenacibaculum and Hyphomonas was decreased. These variations of bacterial community structure were ascribed to the dissolution of nutrients N and P. Comparing the results of different experimental groups, this study concluded that dust storm improved the abundance of heterotrophic bacteria by dissolution of N and Fe.
文摘From Oct., 1999 to Oct., 2000, the heterotrophic bacterial flora in the aquaculture area around Xuejiadao was investigated. The result shows that the populations of the heterotrophic bacteria are heavier in summer and autumn than those in winter and spring. The average populations in seawater, sediment, the surface of seaweed and the surface of fish are 1.4×10 4 cfu mL -1, 5.4×10 6 cfu g -1, 1.5×10 6 cfu g -1 and 1.8×10 3 cfu cm -2, respectively. A total of 301 strains were isolated, among them 259 were Gram-negative. All the Gram-negative bacteria belong to 13 genera and some genera of Enterobacteriaceae. The communities of bacteria are slightly different among the samples. In the body surface of fish, Genus vibrio is dominant. In the remaining samples, dominant genus is Aeromonas.