Hydro-Pericardium Hepatitis (HPH) is an emerging infectious disease of commercial poultry, caused by different serotypes of Fowl Adeno Virus. The vertical transmission of the virus into the progeny may results in deva...Hydro-Pericardium Hepatitis (HPH) is an emerging infectious disease of commercial poultry, caused by different serotypes of Fowl Adeno Virus. The vertical transmission of the virus into the progeny may results in devastating damage, causing huge economic losses to its farmers. In present study, molecular typing is performed on basis of partially conserved hexon gene sequences, using a unique set of primers having common reverse oligo for simultaneous detection of FAdV1, FAdV-4 and FAdV-11. A total of 14 fowl adeno virus strains were isolated from 100 suspected adeno virus liver samples, collected from different districts in Pakistan, between 2018 and 2019. FASTA’s sequence alignment and phylogenetic analysis revealed that out of the 14, one isolate which belonged to group A showed 27% similarity with FAdV-1, while three isolates showed 99%, 95% & 45% similarity to FAdV-4 (Group C). Whereas, ten isolates showed more than 99% similarity to FAdV-11 (Group D). The serotypes FAdV1, FAdV-4 and FAdV-11 are prevailing in the breeder and broilers. These results hold great importance in rapid, reliable and simultaneous detection of the three FAdV serotypes. Therefore, fowl adeno virus vaccine production for commercial poultry shall be according to the prevalent field serotypes.展开更多
The plasmid PE which harbored the whole hexon-encoding gene of egg drop syndrome virus(EDSV) was identified and the hexon protein gene was obtained from it by restriction endonucleases.The gene was then directionally ...The plasmid PE which harbored the whole hexon-encoding gene of egg drop syndrome virus(EDSV) was identified and the hexon protein gene was obtained from it by restriction endonucleases.The gene was then directionally inserted into the PphI/KpnI sites of pQE32 and fused with the 6×His gene.This recombinant plasmid was transformed into E.coli M15 for expression and induced with IPTG.It was demonstrated by SDS-PAGE and Western blot that one expressed protein,110kD in size,could be specifically recognized by polyclonal anti-EDSV serum.The objective product was principally soluble and accounted for 21% of the total cellular proteins.The protein was used to detect the existence of antiEDSV IgG in 41 clinical chicken sera by agar diffusion test,and the result was in accordance with that when EDSV was used as antigen.These results showed that the expressed hexon recombinant protein can be used as diagnostic antigen of EDSV.展开更多
文摘Hydro-Pericardium Hepatitis (HPH) is an emerging infectious disease of commercial poultry, caused by different serotypes of Fowl Adeno Virus. The vertical transmission of the virus into the progeny may results in devastating damage, causing huge economic losses to its farmers. In present study, molecular typing is performed on basis of partially conserved hexon gene sequences, using a unique set of primers having common reverse oligo for simultaneous detection of FAdV1, FAdV-4 and FAdV-11. A total of 14 fowl adeno virus strains were isolated from 100 suspected adeno virus liver samples, collected from different districts in Pakistan, between 2018 and 2019. FASTA’s sequence alignment and phylogenetic analysis revealed that out of the 14, one isolate which belonged to group A showed 27% similarity with FAdV-1, while three isolates showed 99%, 95% & 45% similarity to FAdV-4 (Group C). Whereas, ten isolates showed more than 99% similarity to FAdV-11 (Group D). The serotypes FAdV1, FAdV-4 and FAdV-11 are prevailing in the breeder and broilers. These results hold great importance in rapid, reliable and simultaneous detection of the three FAdV serotypes. Therefore, fowl adeno virus vaccine production for commercial poultry shall be according to the prevalent field serotypes.
文摘The plasmid PE which harbored the whole hexon-encoding gene of egg drop syndrome virus(EDSV) was identified and the hexon protein gene was obtained from it by restriction endonucleases.The gene was then directionally inserted into the PphI/KpnI sites of pQE32 and fused with the 6×His gene.This recombinant plasmid was transformed into E.coli M15 for expression and induced with IPTG.It was demonstrated by SDS-PAGE and Western blot that one expressed protein,110kD in size,could be specifically recognized by polyclonal anti-EDSV serum.The objective product was principally soluble and accounted for 21% of the total cellular proteins.The protein was used to detect the existence of antiEDSV IgG in 41 clinical chicken sera by agar diffusion test,and the result was in accordance with that when EDSV was used as antigen.These results showed that the expressed hexon recombinant protein can be used as diagnostic antigen of EDSV.
