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Broadband high-efficiency dielectric metalenses based on quasi-continuous nanostrips 被引量:1
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作者 Xiaohu Zhang Qinmiao Chen +6 位作者 Dongliang Tang Kaifeng Liu Haimo Zhang Lintong Shi Mengyao He Yongcai Guo Shumin Xiao 《Opto-Electronic Advances》 SCIE EI CAS CSCD 2024年第5期43-51,共9页
Benefiting from the abrupt phase changes within subwavelength thicknesses,metasurfaces have been widely applied for lightweight and compact optical systems.Simultaneous broadband and high-efficiency characteristics ar... Benefiting from the abrupt phase changes within subwavelength thicknesses,metasurfaces have been widely applied for lightweight and compact optical systems.Simultaneous broadband and high-efficiency characteristics are highly attractive for the practical implementation of metasurfaces.However,current metasurface devices mostly adopt discrete micro/nano structures,which rarely realize both merits simultaneously.In this paper,dielectric metasurfaces composed of quasi-continuous nanostrips are proposed to overcome this limitation.Via quasi-continuous nanostrips metasurface,a normal focusing metalens and a superoscillatory lens overcoming the diffraction limit are designed and experimentally demonstrated.The quasi-continuous metadevices can operate in a broadband wavelength ranging from 450 nm to 1000nm and keep a high power efficiency.The average efficiency of the fabricated metalens reaches 54.24%,showing a significant improvement compared to the previously reported metalenses with the same thickness.The proposed methodology can be easily extended to design other metadevices with the advantages of broadband and high-efficiency in practical optical systems. 展开更多
关键词 BROADBAND high-efficiency metalens sub-diffraction QUASI-CONTINUOUS
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High-efficiency Carbonation Modification Methods of Recycled Coarse Aggregates
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作者 张美香 YANG Xiaolin +3 位作者 丁亚红 SUN Bo ZHANG Xianggang LÜXiuwen 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS CSCD 2024年第2期386-398,共13页
To solve the problem of only surface carbonation and realize high-efficiency carbonation of recycled coarse aggregate,the method of carbonated recycled coarse aggregate with nano materials pre-soaking was first put fo... To solve the problem of only surface carbonation and realize high-efficiency carbonation of recycled coarse aggregate,the method of carbonated recycled coarse aggregate with nano materials pre-soaking was first put forward.The carbonation effect of modified recycled coarse aggregate with three different carbonation methods was evaluated,and water absorption,apparent density and crush index of modified recycled coarse aggregate were measured.Combined with XRD,SEM,and MIP microscopic analysis,the high-efficiency carbonation strengthening mechanism of modified recycled coarse aggregate was revealed.The experimental results show that,compared with the non-carbonated recycled coarse aggregate,the physical and microscopic properties of carbonated recycled coarse aggregate are improved.The method of carbonation with nano-SiO_(2) pre-soaking can realize the high-efficiency carbonation of recycled coarse aggregate,for modified recycled coarse aggregate with the method,water absorption is reduced by 23.03%,porosity is reduced by 44.06%,and the average pore diameter is 21.82 nm.The high-efficiency carbonation strengthening mechanism show that the pre-socked nano-SiO_(2) is bound to the hydration product Ca(OH)_(2) of the old mortar with nano-scale C-S-H,which can improve the CO_(2) absorption rate,accelerate the carbonation reaction,generate more stable CaCO_(3) and nano-scale silica gel,and bond to the dense three-dimensional network structure to realize the bidirectional enhancement of nano-materials and pressurized carbonation.It is concluded that the method of carbonation with nano-SiO_(2) pre-soaking is a novel high-efficiency carbonation modification of recycled coarse aggregate. 展开更多
关键词 recycled coarse aggregate pressurized carbonation high-efficiency carbonation NANO-SIO2 strengthening mechanism
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Toward high-efficiency perovskite solar cells with one-dimensional oriented nanostructured electron transport materials 被引量:1
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作者 Yinhua Lv Bing Cai +3 位作者 Ruihan Yuan Yihui Wu Quinn Qiao Wen-Hua Zhang 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2023年第7期66-87,I0003,共23页
The unique advantages of one-dimensional(1D)oriented nanostructures in light-trapping and chargetransport make them competitive candidates in photovoltaic(PV)devices.Since the emergence of perovskite solar cells(PSCs)... The unique advantages of one-dimensional(1D)oriented nanostructures in light-trapping and chargetransport make them competitive candidates in photovoltaic(PV)devices.Since the emergence of perovskite solar cells(PSCs),1D nanostructured electron transport materials(ETMs)have drawn tremendous interest.However,the power conversion efficiencies(PCEs)of these devices have always significantly lagged behind their mesoscopic and planar counterparts.High-efficiency PSCs with 1D ETMs showing efficiency over 22%were just realized in the most recent studies.It yet lacks a comprehensive review covering the development of 1D ETMs and their application in PSCs.We hence timely summarize the advances in 1D ETMs-based solar cells,emphasizing on the fundamental and optimization issues of charge separation and collection ability,and their influence on PV performance.After sketching the classification and requirements for high-efficiency 1D nanostructured solar cells,we highlight the applicability of 1D TiO_(2)nanostructures in PSCs,including nanotubes,nanorods,nanocones,and nanopyramids,and carefully analyze how the electrostatic field affects cell performance.Other kinds of oriented nanostructures,e.g.,ZnO and SnO_(2)ETMs,are also described.Finally,we discuss the challenges and propose some potential strategies to further boost device performance.This review provides a broad range of valuable work in this fast-developing field,which we hope will stimulate research enthusiasm to push PSCs to an unprecedented level. 展开更多
关键词 1D nanostructures Perovskite solar cells Electron transport materials Electrostatic field high-efficiency
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鸽新城疫病毒可视化LAMP检测方法的建立与应用 被引量:1
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作者 唐艳荣 张小梅 +3 位作者 郭建立 曹院章 张海云 李壹 《中国家禽》 北大核心 2024年第3期120-124,共5页
为建立一种简便、快速,适用于基层检测鸽Ⅰ型副黏病毒的方法,研究针对鸽Ⅰ型副黏病毒全基因组保守序列,设计了1组可检测基因Ⅵ、Ⅶ、ⅩⅩ、ⅩⅪ型分离株的环介导等温扩增(LAMP)引物,通过优化反应条件及体系,建立鸽Ⅰ型副黏病毒可视化LAM... 为建立一种简便、快速,适用于基层检测鸽Ⅰ型副黏病毒的方法,研究针对鸽Ⅰ型副黏病毒全基因组保守序列,设计了1组可检测基因Ⅵ、Ⅶ、ⅩⅩ、ⅩⅪ型分离株的环介导等温扩增(LAMP)引物,通过优化反应条件及体系,建立鸽Ⅰ型副黏病毒可视化LAMP检测方法。结果显示:建立的鸽Ⅰ型副黏病毒可视化LAMP检测方法可检测基因Ⅵ、Ⅶ、ⅩⅩ、ⅩⅪ型鸽新城疫病毒,与H9亚型禽流感病毒、马立克病病毒、禽网状内皮组织增殖病病毒、小鹅瘟病毒以及禽白血病病毒以及新城疫病毒La Sota疫苗株均无交叉反应,最低检测限为10^(1)copies/μL。研究表明所建立的鸽Ⅰ型副黏病毒可视化LAMP检测方法快速、简便,特异性强,灵敏度高,可应用于基层快速检测鸽Ⅰ型副黏病毒。 展开更多
关键词 鸽新城疫 lamp 可视化 检测
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Selection of Greenhouse Zucchini Varieties and High-Quality,High-Yield and High-Efficiency Cultivation Techniques
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作者 Haijuan ZHANG Guanghui FENG +3 位作者 Lifeng YANG Bo GENG Xiangying HOU Dongwen SUN 《Asian Agricultural Research》 2023年第6期38-40,共3页
[Objectives]To select zucchini varieties suitable for cultivation in Zibo City and test its high-yield cultivation techniques.[Methods]Six zucchini varieties were introduced,and their commercial quality and yield were... [Objectives]To select zucchini varieties suitable for cultivation in Zibo City and test its high-yield cultivation techniques.[Methods]Six zucchini varieties were introduced,and their commercial quality and yield were determined.[Results]The yield of Shengfeier,Xiuyu 170 and Xihulu 309 increased by 11.4%,6.9%and 4.6%,respectively compared with S68(control),and zucchini was straight,looked pleasing to the eye,and had strong disease resistance.[Conclusions]The zucchini varieties were selected and the high-quality,high-yield and high-efficiency cultivation techniques were integrated. 展开更多
关键词 ZUCCHINI VARIETY HIGH-QUALITY high-yield and high-efficiency Cultivation techniques
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鸡圆圈病毒3型可视化LAMP检测方法的建立及应用
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作者 张民秀 谢芝勋 +6 位作者 张艳芳 谢志勤 谢丽基 李孟 罗思思 曾婷婷 王粲 《河南农业科学》 北大核心 2024年第4期128-136,共9页
建立鸡圆圈病毒3型(GyV3)可视化环介导等温扩增(LAMP)检测方法,为临床上快速检测鸡GyV3提供一种诊断方法。参考GyV3的VP3基因序列,设计1套LAMP特异性引物,分别通过温度优化、引物浓度优化和反应时间的优化,确定鸡GyV3可视化LAMP诊断方... 建立鸡圆圈病毒3型(GyV3)可视化环介导等温扩增(LAMP)检测方法,为临床上快速检测鸡GyV3提供一种诊断方法。参考GyV3的VP3基因序列,设计1套LAMP特异性引物,分别通过温度优化、引物浓度优化和反应时间的优化,确定鸡GyV3可视化LAMP诊断方法的扩增温度、引物浓度和反应时间,通过特异性试验、敏感性试验、重复性试验和临床样品检测,验证鸡GyV3可视化LAMP诊断方法的特异性、敏感性、稳定性和可靠性。结果显示,鸡GyV3 LAMP检测方法的反应体系:总体积为20.0μL,包括DNA模板1.0μL,2×Master Mix 10.0μL,内引物GyV3-FIP和GyV3-BIP混合液(工作浓度16.0μmol/L)2.0μL,外引物GyV3-F3和GyV3-B3混合液(工作浓度2.0μmol/L)2.0μL,环引物GyV3-LF和GyV3-LB混合液(工作浓度8.0μmol/L)2.0μL,ddH_(2)O 3μL;鸡GyV3 LAMP检测方法的扩增程序:66℃反应20 min,80℃灭活5 min。鸡GyV3可视化LAMP检测方法能特异性检测GyV3,最低检测浓度为10^(1)拷贝/μL,组内和组间变异系数小于5%;与常规PCR方法检测临床样品的结果比较,两者结果符合率达100%。建立的鸡GyV3可视化LAMP检测方法能特异性检测GyV3,具有特异性好、敏感性高及污染小等优点,且检测结果可通过肉眼观察进行判断,适用于GyV3的临床快速筛查。 展开更多
关键词 鸡圆圈病毒3型(GyV3) 可视化lamp 特异性引物 诊断方法 敏感性
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虾类十足目虹彩病毒1(DIV1)LAMP-HNB快检方法的建立
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作者 王雯琼 葛明峰 +4 位作者 卢先东 刘艳红 斯烈钢 申屠基康 徐胜威 《安徽农业科学》 CAS 2024年第15期192-196,共5页
[目的]建立一种操作简便、快速、可视化的虾类病原检测方法,实现基层养殖户自检。[方法]针对十足目虹彩病毒1(DIV1),设计环介导等温扩增(LAMP)特异性引物,对反应试剂用量、温度、反应时间等条件进行优化,建立LAMP与羟基萘酚蓝(HNB)结合... [目的]建立一种操作简便、快速、可视化的虾类病原检测方法,实现基层养殖户自检。[方法]针对十足目虹彩病毒1(DIV1),设计环介导等温扩增(LAMP)特异性引物,对反应试剂用量、温度、反应时间等条件进行优化,建立LAMP与羟基萘酚蓝(HNB)结合的LAMP-HNB可视化快速检测方法。[结果]在LAMP反应体系中添加12 mmol/L Mg^(2+)和250μmol/L HNB,65℃恒温条件下反应60 min最佳;DIV1病原质粒最低检出限为102 copies/μL,稳定性和特异性较好,通过带有靶标基因的阳性质粒进行16次重复性试验结果均较为稳定,多种模板扩增试验均未发生假阳性情况,符合试验要求。[结论]该研究建立的LAMP-HNB可视化快速检测方法,适用于上述对虾DIV1的现场快速检测。 展开更多
关键词 南美白对虾 lamp HNB 十足目虹彩病毒1 快速检测
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Security and Privacy in Solar Insecticidal Lamps Internet of Things:Requirements and Challenges
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作者 Qingsong Zhao Lei Shu +3 位作者 Kailiang Li Mohamed Amine Ferrag Ximeng Liu Yanbin Li 《IEEE/CAA Journal of Automatica Sinica》 SCIE EI CSCD 2024年第1期58-73,共16页
Solar insecticidal lamps(SIL) can effectively control pests and reduce the use of pesticides. Combining SIL and Internet of Things(IoT) has formed a new type of agricultural IoT,known as SIL-IoT, which can improve the... Solar insecticidal lamps(SIL) can effectively control pests and reduce the use of pesticides. Combining SIL and Internet of Things(IoT) has formed a new type of agricultural IoT,known as SIL-IoT, which can improve the effectiveness of migratory phototropic pest control. However, since the SIL is connected to the Internet, it is vulnerable to various security issues.These issues can lead to serious consequences, such as tampering with the parameters of SIL, illegally starting and stopping SIL,etc. In this paper, we describe the overall security requirements of SIL-IoT and present an extensive survey of security and privacy solutions for SIL-IoT. We investigate the background and logical architecture of SIL-IoT, discuss SIL-IoT security scenarios, and analyze potential attacks. Starting from the security requirements of SIL-IoT we divide them into six categories, namely privacy, authentication, confidentiality, access control, availability,and integrity. Next, we describe the SIL-IoT privacy and security solutions, as well as the blockchain-based solutions. Based on the current survey, we finally discuss the challenges and future research directions of SIL-IoT. 展开更多
关键词 CHALLENGES Internet of Things(IoT) privacy and security security requirements solar insecticidal lamps(SIL)
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Biomass Control via UV Lamp Application and Nutrient Supply Restriction in the Biofiltration of Gaseous VOCs
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作者 Takashi Higuchi Kokoro Hashizume 《Journal of Environmental Science and Engineering(B)》 CAS 2024年第3期97-104,共8页
Biofiltration may have clogging problems owing to excess biomass growth during the treatment of gaseous pollutants.In this study,we employed an UV(Ultraviolet)lamp and controlled the nutrient supply to conduct a biofi... Biofiltration may have clogging problems owing to excess biomass growth during the treatment of gaseous pollutants.In this study,we employed an UV(Ultraviolet)lamp and controlled the nutrient supply to conduct a biofiltration process for treating 2-butanone(MEK:Methyl Ethyl Ketone)and toluene in a gas stream.Two methods of UV lamp usage(direct and indirect irradiation)and several nutrient supply methods were tested.However,no clear effect was observed with either UV usage.Under the optimal conditions,97%of the MEK and 69%of the toluene gases were removed after 29 s of EBRT(Empty Bed Retention Time).The inlet loads were 18 and 19 mg/(m^(3)·h)for MEK and toluene,respectively.Under these conditions,23 g-N/(m^(3)·day)of nitrate-nitrogen was consumed.Excess biomass growth occurred during simultaneous excess nutrient supply and a persistent irrigation schedule.In this study,we demonstrated the effective use of a dense nitrate solution to deliver an appropriate amount of nutrients and moisture,and the optimal irrigation frequency was four times per week. 展开更多
关键词 BIOFILTRATION gaseous VOCs(Volatile Organic Compounds) nutrient supply UV lamp ozone
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Observation of therapeutic effect of lamp irradiation combined with purple gromwell oil gauze on alleviating intestinal colic in patients
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作者 Bi-Zhi Cen Yin-Song Chen +2 位作者 Li-Ping Li Jing-Wen Wu Yan-Fen Xie 《World Journal of Gastrointestinal Surgery》 SCIE 2024年第6期1749-1755,共7页
BACKGROUND Intestinal colic is a common complication in patients who have undergone radical surgery for colorectal cancer.Traditional Chinese medicine has advantages,including safety and stability,for the treatment of... BACKGROUND Intestinal colic is a common complication in patients who have undergone radical surgery for colorectal cancer.Traditional Chinese medicine has advantages,including safety and stability,for the treatment of intestinal colic.Lamp irra-diation for abdominal ironing has been applied in the treatment of many gas-trointestinal diseases.Purple gromwell oil has the effects of clearing heat,cooling blood,reducing swelling,and relieving pain.RESULTS The general effective rate in the observation group was 95.00%,which was significantly higher than that in the control group(86.67%,P<0.05).Before treatment,there was no significant difference in the duration of symptoms between the groups(P>0.05).After 1,2,3,and 4 d of treatment,the duration of symptoms in both groups were decreased,and the duration in the observation group was significantly lower than that in the control group(96.54±9.57 vs 110.45±11.23,87.26±12.07 vs 104.44±11.68,80.45±16.21 vs 99.44±14.95,73.18±15.58 vs 92.17±14.20;P<0.05).After 1,3,5,and 7 d of treatment,the NRS scores in both groups were decreased,and the NRS scores in the observation group were significantly lower than those in the control group(3.56±0.41 vs 4.04±0.58,3.07±0.67 vs 3.74±1.02,2.52±0.76 vs 3.43±0.85,2.03±0.58 vs 3.03±0.82;P<0.05).There was no significant difference in the rate of adverse reaction occurrence between the groups(P>0.05).CONCLUSION The use of lamp irradiation combined with purple gromwell oil gauze in patients with intestinal colic after radical surgery for colorectal cancer can reduce symptom duration,alleviate intestinal colic,and improve treatment efficacy,and this approach is safe.It is worth promoting the use of this treatment in clinical practice. 展开更多
关键词 lamp irradiation Purple gromwell oil gauze Intestinal colic Radical surgery for colorectal cancer Therapeutic effect
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小反刍兽疫LAMP反应结果可视化检测方法比较研究
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作者 索婧媛 郑茜之 +1 位作者 王博 刘学东 《野生动物学报》 北大核心 2024年第3期664-669,共6页
环介导等温扩增(loop-mediated isothermal amplification,LAMP)技术是一种可应用于各种病原体检测、基因分型等领域的等温扩增技术。该技术所需设备简单且易于操作,因此具有较大的发展潜力。根据目前已经存在的判定LAMP结果的可视化方... 环介导等温扩增(loop-mediated isothermal amplification,LAMP)技术是一种可应用于各种病原体检测、基因分型等领域的等温扩增技术。该技术所需设备简单且易于操作,因此具有较大的发展潜力。根据目前已经存在的判定LAMP结果的可视化方法,以可能对野生小反刍动物造成威胁的小反刍兽疫病毒(Peste des petits ruminants virus,PPRV)cDNA为检测对象,选择合适的引物组,比较评估均可在日光或紫外光下区分阴阳性结果的SYBR Green I、SYBR Safe Stain和羟基萘酚蓝(hydroxy naphthol blue,HNB)3种指示剂方法的适用性与灵敏度。结果表明:3种方法的灵敏度都很高,均与琼脂糖凝胶电泳相当。其中,SYBR Green I结果区分度高、不受体系成分影响且无须额外设备,但成本较高;SYBR Safe Stain具有良好的结果区分度,成本相对较低,但需要额外的紫外照射设备;HNB成本最低,但颜色区分度较低,且易受多种因素影响。建议在选择LAMP指示剂时,综合考虑成本、使用便利性、结果准确性及实验条件,通过充分的体系优化和验证,确保LAMP检测的准确性和可靠性。研究结果突显了不同方法的特征和适用场景,为研究人员根据不同场所与条件选择高效、便捷的LAMP反应结果可视化方法提供参考。 展开更多
关键词 环介导等温扩增 SYBR Green I SYBR Safe Stain 羟基萘酚蓝 小反刍兽疫病毒
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传统方法、荧光定量PCR法、LAMP法检测痰液标本中肺炎链球菌的价值
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作者 段影杰 张皓 黄利美 《医学理论与实践》 2024年第16期2806-2808,共3页
目的:分析传统方法、荧光定量聚合酶链反应(PCR)法、环介导恒温扩增技术(LAMP)法检测痰液标本中肺炎链球菌的价值。方法:选取2022年1月—2023年7月医院收治的100例肺炎患者,均按相同方法严格采集患者的痰液标本后,采用痰培养法、荧光定... 目的:分析传统方法、荧光定量聚合酶链反应(PCR)法、环介导恒温扩增技术(LAMP)法检测痰液标本中肺炎链球菌的价值。方法:选取2022年1月—2023年7月医院收治的100例肺炎患者,均按相同方法严格采集患者的痰液标本后,采用痰培养法、荧光定量PCR法、LAMP法检测肺炎链球菌,分析不同检测方法的检测价值。结果:100例肺炎患者痰液标本经痰细菌培养法、荧光定量PCR法、LAPM法检测结果比较,差异无统计学意义(P>0.05),与痰细菌培养法相比,荧光定量PCR法、LAPM法对肺炎链球菌检测一致性高(Kappa=0.826、0.830,P<0.05)。以痰细菌培养法作为金标准,荧光定量PCR法、LAPM法对肺炎链球菌检测价值比较,无统计学差异(P>0.05)。结论:传统方法、荧光定量PCR法、LAMP法检测痰液标本中肺炎链球菌均有较高价值。 展开更多
关键词 痰培养法 荧光定量PCR法 lamp 肺炎链球菌
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基于CP基因的云南烟草花叶病毒RT-LAMP快速检测体系的构建
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作者 赵正婷 盖晓彤 +3 位作者 张俊蕾 卢灿华 姜宁 刘雅婷 《山东农业科学》 北大核心 2024年第5期154-162,共9页
为快速检测云南烟草花叶病毒(Tobacco mosaic virus,TMV),本研究根据来自云南烟草TMV分离物的外壳蛋白(CP)基因保守核苷酸序列,设计了5组引物进行筛选,并采用单一变量法对反应的温度、时间、甜菜碱浓度、dNTPs浓度、Mg2+浓度及内、外引... 为快速检测云南烟草花叶病毒(Tobacco mosaic virus,TMV),本研究根据来自云南烟草TMV分离物的外壳蛋白(CP)基因保守核苷酸序列,设计了5组引物进行筛选,并采用单一变量法对反应的温度、时间、甜菜碱浓度、dNTPs浓度、Mg2+浓度及内、外引物浓度比等进行逐一优化,建立了云南烟草TMV逆转录环介导等温扩增(RT-LAMP)检测体系。结果表明,最佳引物组为第4组,最适反应温度为60℃,甜菜碱、dNTPs、Mg^(2+)的最佳反应浓度分别为0.6、0.4、2.0 mmol/L,最佳内、外引物浓度比为4∶1,最佳反应时间40 min。优化后的RT-LAMP经SYBR Green I染色可肉眼判断结果,特异性高,灵敏度是常规RT-PCR的10倍。RT-LAMP检测体系的建立为云南烟草TMV的检测提供了一种便捷、高效、可靠的方法。 展开更多
关键词 烟草花叶病毒 CP基因 RT-lamp 特异性 灵敏度
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High-efficiency Planting Techniques of Five Crops a Year for Fresh Edible "Faba Bean/Spring Mazie+Soybean-Autumn Maize/Autumn Soybean"
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作者 葛红 汪凯华 +4 位作者 王学军 陈满峰 缪亚梅 顾春燕 单志良 《Agricultural Science & Technology》 CAS 2016年第4期833-837,共5页
The high-efficiency planting mode for five crops a year of fresh edible "faba bean/spring maize+soybean-autumn maize/autumn soybean" was introduced, and its yield and economic benefits were compared with the planti... The high-efficiency planting mode for five crops a year of fresh edible "faba bean/spring maize+soybean-autumn maize/autumn soybean" was introduced, and its yield and economic benefits were compared with the planting mode of three crops a year of "faba bean-spring maize/red bean". The results showed that the planting method for fresh edible "faba bean/spring maize+soybean-autumn maize/autumn soybean" was much easier to operate with the input-output ratio of about185.6%, and its yield and economic benefits were 2.09 and 1.83 times of that of the planting mode for three crops a year, significantly improving the agricultural yield and income of farmers. In addition, the cropping index of the planting mode for fresh edible fresh edible "faba bean/spring maize +soybean-autumn maize/autumn soybean" reached up to 350%, and planting faba bean once a year and soybean twice a year could make the biological fixation amount of nitrogen increase 350-450kg/m^2, which equaled to up to 700 kg/m^2 of urea, showing significant ecological and social benefits. Based on the comparison results, the high-yield culture techniques of the planting mode of fresh edible "faba bean/spring maize+soybean-autumn maize/autumn soybean" were summarized. 展开更多
关键词 Fresh edible faba bean Fresh edible maize Fresh edible soybean Five crops a year high-efficiency planting mode
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南洋臀纹粉蚧生物学特性及LAMP检测
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作者 黄鹏 张杰 +2 位作者 姚锦爱 蓝炎阳 余德亿 《昆虫学报》 CAS CSCD 北大核心 2023年第8期1052-1062,共11页
【目的】明确南洋臀纹粉蚧Planococcus lilacinus生物学特性及实现对该虫的可视化检测。【方法】室内观察记录南洋臀纹粉蚧不同发育阶段的形态特征和历期及雌成虫的繁殖能力;以南洋臀纹粉蚧28S rDNA序列为靶标,设计其特异性的环介导等... 【目的】明确南洋臀纹粉蚧Planococcus lilacinus生物学特性及实现对该虫的可视化检测。【方法】室内观察记录南洋臀纹粉蚧不同发育阶段的形态特征和历期及雌成虫的繁殖能力;以南洋臀纹粉蚧28S rDNA序列为靶标,设计其特异性的环介导等温扩增(loop-mediated isothermal amplification,LAMP)引物组用于建立相应的可视化检测方法,并进行特异性、灵敏度及稳定性验证。【结果】南洋臀纹粉蚧雌雄虫具有不同的世代生活史,卵初产时即可见虫体轮廓,并具1对暗红色单眼;1龄若虫行动活泼,雌雄难辨;2龄若虫体缘出现白色细蜡棒,雌雄体型出现分化;3龄若虫仅存在雌虫,形似雌成虫;蛹仅存在雄虫,分为预蛹期和蛹期;雌成虫体表覆盖厚实的白色蜡粉,体缘18对粗蜡棒突出明显;雄成虫有一对透明发达的前翅,腹部末端具1对白色长蜡丝。该虫卵历期(1.37±0.26)h,雌成虫历期(32.67±4.82)d,各龄若虫、蛹和雄成虫历期均不超过(7.65±0.82)d;两性生殖的单雌产卵量和卵孵化率分别为(531.92±69.98)粒和96.02%±1.35%,孤雌生殖的单雌产卵量和卵孵化率分别为(403.50±71.11)粒和88.41%±3.03%。建立的LAMP可视化检测方法能有效扩增南洋臀纹粉蚧DNA,最佳反应温度和时间及最低检测浓度分别为66℃、55 min和100 fg/μL,反应产物呈绿色阳性,检出率达91.67%~100.00%,但无法扩增其他7种近缘种属粉蚧DNA,反应产物呈橙黄色阴性。【结论】南洋臀纹粉蚧雌雄二型,生长发育快,繁殖力强,急需作好其检测与监测工作;该虫的LAMP可视化检测方法便捷高效、特异性强、灵敏度高且稳定性好,有望用于非专业鉴定人员对该虫的现场快速、准确识别。 展开更多
关键词 南洋臀纹粉蚧 形态特征 发育繁殖 lamp 可视化检测
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智慧校园视域下基于LAMP的在线考试系统敏捷设计与实现
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作者 余久久 张继山 +4 位作者 吴宁 胡雪 解雪杨 刘丽 张错玲 《西昌学院学报(自然科学版)》 2023年第4期48-54,共7页
智慧校园是高校信息化建设发展的趋势。为了更好地提供高校教师出(组)卷管理、学生在线考试与试卷提交等功能,从当前主流智慧校园所具备的功能体系视角,依托本地试点A高校智慧校园微服务与微应用技术架构,采用软件敏捷开发模型Scrum,快... 智慧校园是高校信息化建设发展的趋势。为了更好地提供高校教师出(组)卷管理、学生在线考试与试卷提交等功能,从当前主流智慧校园所具备的功能体系视角,依托本地试点A高校智慧校园微服务与微应用技术架构,采用软件敏捷开发模型Scrum,快速设计并实现出一个基于LAMP(Linux+Apache+MySQL+PHP)的在线考试系统。该系统服务器端共享A高校校园应用数据中心,客户端采用PHP工具开发,系统性能稳定,操作便捷。最后提出了后续研究工作:为该考试系统建立统一的校园微信公众号以增强用户体验;构建基于云计算的移动互联“办事大厅”以提升消息响应速度。 展开更多
关键词 智慧校园 敏捷开发模型Scrum 在线考试系统 lamp 微服务与微应用
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基于原位LAMP技术的牡蛎疱疹病毒(OsHV-1)易感宿主调查 被引量:3
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作者 张翔 谷莉 +5 位作者 郑玉东 李晨 白昌明 辛鲁生 王崇明 刘金兰 《渔业科学进展》 CSCD 北大核心 2023年第2期174-185,共12页
牡蛎疱疹病毒(Ostreidherpesvirus1,Os HV-1)给世界双壳贝类养殖业造成了严重的经济损失。10余种双壳贝类陆续被认定为易感宿主,仍有其他几种贝类仅有PCR核酸阳性数据,因确诊证据不足导致其易感性未得到充分评估。原位环介导等温核酸扩... 牡蛎疱疹病毒(Ostreidherpesvirus1,Os HV-1)给世界双壳贝类养殖业造成了严重的经济损失。10余种双壳贝类陆续被认定为易感宿主,仍有其他几种贝类仅有PCR核酸阳性数据,因确诊证据不足导致其易感性未得到充分评估。原位环介导等温核酸扩增(LAMP)检测技术相对传统原位杂交技术具有灵敏度高、方便快捷、可作为病原微生物感染证据的优点。为了在Os HV-1流行病学调查过程中实现病毒感染的快速检测和确诊,根据已报道的Os HV-1特异性LAMP检测引物,设计内引物,优化反应条件,建立了Os HV-1的原位LAMP检测方法。基于该方法对2019年以来采集的长牡蛎(Crassostreagigas)、福建牡蛎(Crassostrea angulata)、栉孔扇贝(Chlamysfarreri)、虾夷扇贝(Mizuhopecten yessoensis)、毛蚶(Scapharca subcrenata)和菲律宾蛤仔(Ruditapes philippinarum)样本进行检测。结果显示,毛蚶样本的Os HV-1原位LAMP检测结果呈阳性;其他几种贝类部分样本的实时定量PCR (qPCR)检测呈阳性,但原位LAMP检测呈阴性。对毛蚶样本的原位LAMP检测结果分析发现,病毒杂交信号主要分布在外套膜和肝胰腺等器官的结缔组织,推测感染的细胞为成纤维细胞和血淋巴细胞;在闭壳肌和斧足肌肉组织的肌细胞细胞核中也发现较多杂交信号。鳃丝内和周边偶现阳性信号,推测来自渗出的血淋巴细胞。基于原位LAMP技术的Os HV-1检测结果显示,毛蚶是Os HV-1的一种易感宿主,毛蚶结缔组织、肌肉组织和血淋巴细胞对该病毒有强亲嗜性。 展开更多
关键词 牡蛎疱疹病毒 原位lamp 易感性 流行病学调查
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二重荧光RT-LAMP鉴别检测禽呼肠孤病毒和鸡滑液囊支原体
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作者 曾婷婷 谢芝勋 +9 位作者 谢丽基 王盛 谢志勤 黄娇玲 万丽军 任红玉 张艳芳 张民秀 范晴 邓显文 《畜牧与兽医》 CAS 北大核心 2023年第9期66-75,共10页
本研究旨在建立一套通过添加荧光探针FD,反应后可使用成像仪直接观测结果的鉴别检测禽呼肠孤病毒(ARV)和鸡滑液囊支原体(MS)的二重荧光RT-LAMP检测方法。根据ARV和MS的保守基因片段,设计了2套LAMP引物,并各添加1条FD荧光探针,2条FD探针... 本研究旨在建立一套通过添加荧光探针FD,反应后可使用成像仪直接观测结果的鉴别检测禽呼肠孤病毒(ARV)和鸡滑液囊支原体(MS)的二重荧光RT-LAMP检测方法。根据ARV和MS的保守基因片段,设计了2套LAMP引物,并各添加1条FD荧光探针,2条FD探针分别标记CY5和6-FAM荧光基团。结果:ARV阳性对照在成像仪下发出红色荧光,MS阳性对照发出绿色荧光,双重模板经过图像融合后为黄色荧光;特异性结果显示,该二重荧光RT-LAMP检测方法仅能扩增ARV和MS两种病原,对其他常见病原如鸡毒支原体(MG)、禽流感病毒(AIV)、新城疫病毒(NDV)等无扩增反应;敏感性结果显示,该二重荧光RT-LAMP检测方法对ARV和MS的最低检测量分别为1.7×10^(2)copies和1.9×10^(2)copies;使用该方法检测40份临床样品,检出率与本实验室前期建立的二重荧光RT-PCR检测结果相同。综上,该二重荧光RT-LAMP检测方法可特异、敏感地鉴别检测ARV和MS,通过添加FD荧光探针和成像仪观测结果,为不同条件的实验室提供了新的检测手段。 展开更多
关键词 FD荧光探针 二重荧光RT-lamp 禽呼肠孤病毒 鸡滑液囊支原体
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奶牛乳房炎源肺炎克雷伯菌PCR和LAMP检测方法的建立 被引量:1
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作者 罗阳 田唯嘉 +6 位作者 何芳 浣成 刘伯承 张佰忠 宋武 杨青 易康乐 《河南农业科学》 北大核心 2023年第2期145-150,共6页
为快速鉴定因肺炎克雷伯菌侵染引起的奶牛乳房炎,进一步为该疾病提供更合理的治疗方案,分别以肺炎克雷伯菌16S-23S rDNA内部转录间隔层序列(Internal transcribed spacer,ITS)和脲酶UreD基因为靶点,利用PCR技术和环介导等温扩增技术(Loo... 为快速鉴定因肺炎克雷伯菌侵染引起的奶牛乳房炎,进一步为该疾病提供更合理的治疗方案,分别以肺炎克雷伯菌16S-23S rDNA内部转录间隔层序列(Internal transcribed spacer,ITS)和脲酶UreD基因为靶点,利用PCR技术和环介导等温扩增技术(Loop-mediated isothermal amplification,LAMP)构建奶牛乳房炎源肺炎克雷伯菌快速鉴定方法。结果表明,利用PCR技术扩增ITS序列能准确鉴定出肺炎克雷伯菌,在核酸质量浓度为5×10^(-2)ng/μL的环境中依然能实现稳定扩增;利用LAMP技术扩增UreD基因的最佳反应温度为62℃,其最低检测核酸质量浓度为5×10^(-7)ng/μL,检测灵敏度是PCR方法的105倍。综上,建立了PCR技术和LAMP技术快速鉴定奶牛乳房炎型肺炎克雷伯菌的方法,特异性强且灵敏度高。 展开更多
关键词 奶牛乳房炎 肺炎克雷伯菌 UreD基因 PCR lamp
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Adiabatic cooling for cold polar molecules on a chip using a controllable high-efficiency electrostatic surface trap 被引量:6
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作者 李胜强 许亮 +2 位作者 夏勇 汪海玲 印建平 《Chinese Physics B》 SCIE EI CAS CSCD 2014年第12期148-154,共7页
We propose a controllable high-efficiency electrostatic surface trap for cold polar molecules on a chip by using two insulator-embedded charged rings and a grounded conductor plate. We calculate Stark energy structure... We propose a controllable high-efficiency electrostatic surface trap for cold polar molecules on a chip by using two insulator-embedded charged rings and a grounded conductor plate. We calculate Stark energy structure pattern of ND3 molecules in an external electric field using the method of matrix diagonalization. We analyze how the voltages that are applied to the ring electrodes affect the depth of the efficient well and the controllability of the distance between the trap center and the surface of the chip. To obtain a better understanding, we simulate the dynamical loading and trapping processes of ND3 molecules in a |J, KM = |1,-1 state by using classical Monte–Carlo method. Our study shows that the loading efficiency of our trap can reach ~ 88%. Finally, we study the adiabatic cooling of cold molecules in our surface trap by linearly lowering the potential-well depth(i.e., lowering the trapping voltage), and find that the temperature of the trapped ND3 molecules can be adiabatically cooled from 34.5 m K to ~ 5.8 m K when the trapping voltage is reduced from-35 k V to-3 k V. 展开更多
关键词 CONTROLLABLE high-efficiency surface trap cold polar molecules
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