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Trends and frontiers in signal amplification for aptamer-based tumor detection:A bibliometric analysis
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作者 Dan Cai Gui-Lin Chen +1 位作者 Ting Wang Kun-He Zhang 《World Journal of Clinical Cases》 SCIE 2024年第21期4726-4741,共16页
BACKGROUND Malignant tumors are one of the leading causes of death worldwide,imposing a substantial economic and social burden.Early detection is the key to improving cure rates and reducing mortality rates,which requ... BACKGROUND Malignant tumors are one of the leading causes of death worldwide,imposing a substantial economic and social burden.Early detection is the key to improving cure rates and reducing mortality rates,which requires the development of sensitive early detection technologies.Signal amplification techniques play a crucial role in aptamer-based early detection of tumors and are increasingly garnering attention from researchers.AIM To investigate the current research status,developmental trajectories,and hotspots in signal amplification for aptamer-based tumor detection through bibliometric analysis.METHODS English publications pertaining to signal amplification in aptamer-based tumor detection were retrieved from the Web of Science Core Collection database.VOSviewer and CiteSpace software were employed to analyze various information within this field,including countries,institutions,authors,co-cited authors,journals,co-cited journals,cited references,and keywords.RESULTS A total of 757 publications were included in this study.China accounted for 85.47%of all publications,with Nanjing University(China)emerging as the institution with the highest publication output.The most influential authors and journals were Hasanzadeh M.from Iran and"Biosensors and Bioelectronics",respectively.Exosomes and carcinoembryonic antigen(CEA)stood out as the most researched tumor-related molecules.Currently,the predominant signal amplification technique,nanomaterial,and signal transduction method were identified as hybridization chain reactions,gold nanoparticles,and electrochemical methods,respectively.Over the past 3 years,exosomes,CEA,electrochemical biosensors,and nanosheets have emerged as research hotspots,exhibiting a robust burst of intensity.CONCLUSION This study is the first bibliometric analysis of literature on signal amplification in aptamer-based tumor detection and elucidates the current status,hotspots,and prospective research directions within this realm.Additionally,it provides an important reference for researchers. 展开更多
关键词 APTAMER Signal amplification Tumor BIBLIOMETRICS ELECTROCHEMISTRY NANOSHEET
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A Study of Radiation-Induced Telomere Instability Using Multiplex Ligation-Dependent Probe Amplification (MLPA)
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作者 Alan Chant Ahmad Chaudary Christina M. Kraemer-Chant 《Advances in Biological Chemistry》 CAS 2024年第3期73-85,共13页
The integrity of the chromosomes for two WIL2-derived lymphoblastoid cell lines (TK6 and WTK1) in the presence and absence of ionizing radiation was analyzed by Multiplex Ligation-Dependent Probe Amplification (MLPA).... The integrity of the chromosomes for two WIL2-derived lymphoblastoid cell lines (TK6 and WTK1) in the presence and absence of ionizing radiation was analyzed by Multiplex Ligation-Dependent Probe Amplification (MLPA). The TK6 cell line has the native p53 tumor-suppressor gene, whereas WTK1 cells contain a p53 mutation. Each cell line was isolated pre- and post-irradiation (2 and 3 Gy) and analyzed by MLPA. The impact of irradiation on these two cell lines was investigated using probes that target specific regions on chromosomes associated with subtelomeric regions. Results indicate that WTK1 and TK6 are impacted differently after irradiation, and that each cell line presents its own unique MLPA profile. The most notable differences are the appearance of a number of probes in the post-irradiated MLPA profile that are not present in the controls, and two unique probe signals only seen in WTK1 cells. These results build on our previous studies that indicate how different human cell lines can be affected by radiation in significantly different ways depending on the presence or absence of wild type p53. 展开更多
关键词 Ionizing Radiation Multiplex Ligation-Dependent Probe amplification (MLPA) p53 Tumor Suppressor Telomere Instability
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Modified Sadowski formula-based model for the slope shape amplification effect under multistage slope blasting vibration
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作者 Xiaogang Wu Mingyang Wang +2 位作者 Hao Lu Yongjun Zhang Wen Nie 《International Journal of Mining Science and Technology》 SCIE EI CAS CSCD 2024年第5期631-641,共11页
Blasting operations,which are crucial to open-pit mine production due to their simplicity and efficiency,require precise control through accurate vibration velocity calculations.The conventional Sadowski formula mainl... Blasting operations,which are crucial to open-pit mine production due to their simplicity and efficiency,require precise control through accurate vibration velocity calculations.The conventional Sadowski formula mainly focuses on blast center distance but neglects the amplification effect of blasting vibration waves by terraced terrain,from which the calculated blasting vibration velocities are smaller than the actual values,affecting the safety of the project.To address this issue,our model introduces the influences of slope and time into Sadowski formula to measure safety through blast vibration displacement.In the northern section of the open-pit quartz mine in Jinchang City,Gansu Province,China,the data of a continuous blasting slope project are referred to.Our findings reveal a noticeable vibration amplification effect during blasting when a multi-stage slope platform undergoes a sudden cross-sectional change near the upper overhanging surface.The amplification vibration coefficient increases with height,while vibration waves within rocks decrease from bottom to top.Conversely,platforms without distinct crosssectional changes exhibit no pronounced amplification during blasting.In addition,the vibration intensity decreases with distance as the rock height difference change propagates.The results obtained by the proposed blast vibration displacement equation incorporating slope shape influence closely agree with real-world scenarios.According to Pearson correlation coefficient(PPMCC)analysis,the average accuracy rate of our model is 88.84%,which exceeds the conventional Sadowski formula(46.92%). 展开更多
关键词 Multistage slope Slope shape influence factor Continuous blasting Sadowski formula amplification effect
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Mesenchymal-epithelial transition factor amplification correlates with adverse pathological features and poor clinical outcome in colorectal cancer
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作者 Qiu-Xiao Yu Ping-Ying Fu +2 位作者 Chi Zhang Li Li Wen-Ting Huang 《World Journal of Gastrointestinal Surgery》 SCIE 2024年第5期1395-1406,共12页
BACKGROUND Colorectal cancer(CRC)is the third most common cancer and the second most common cause of cancer-related mortality worldwide.Mesenchymal-epithelial transition factor(MET)gene participates in multiple tumor ... BACKGROUND Colorectal cancer(CRC)is the third most common cancer and the second most common cause of cancer-related mortality worldwide.Mesenchymal-epithelial transition factor(MET)gene participates in multiple tumor biology and shows clinical potential for pharmacological manipulation in tumor treatment.MET amplification has been reported in CRC,but data are very limited.Investigating pathological values of MET in CRC may provide new therapeutic and genetic screening options in future clinical practice.AIM To determine the pathological significance of MET amplification in CRC and to propose a feasible screening strategy.METHODS A number of 205 newly diagnosed CRC patients undergoing surgical resection without any preoperative therapy at Shenzhen Cancer Hospital of Chinese Academy of Medical Sciences were recruited.All patients were without RAS/RAF mutation or microsatellite instability-high.MET amplification and c-MET protein expression were analyzed using fluorescence in situ hybridization(FISH)and immunohistochemistry(IHC),respectively.Correlations between MET aberration and pathological features were detected using the chi-squared test.Progression free survival(PFS)during the two-year follow-up was detected using the Kaplan-Meier method and log rank test.The results of MET FISH and IHC were com pared using one-way ANOVA.RESULTS Polysomy-induced MET amplification was observed in 14.4%of cases,and focal MET amplification was not detected.Polysomy-induced MET amplification was associated with a higher frequency of lymph node metastasis(LNM)(P<0.001)and higher tumor budding grade(P=0.02).In the survival analysis,significant difference was detected between patients with amplified-and non-amplified MET in a two-year follow-up after the first diagnosis(P=0.001).C-MET scores of 0,1+,2+,and 3+were observed in 1.4%,24.9%,54.7%,and 19.0%of tumors,respectively.C-MET overexpression correlated with higher frequency of LNM(P=0.002),but no significant difference of PFS was detected between patients with different protein levels.In terms of concordance between MET FISH and IHC results,MET copy number showed no difference in c-MET IHC 0/1+(3.35±0.18),2+(3.29±0.11)and 3+(3.58±0.22)cohorts,and the MET-to-CEP7 ratio showed no difference in three groups(1.09±0.02,1.10±0.01,and 1.09±0.03).CONCLUSION In CRC,focal MET amplification was a rare event.Polysomy-induced MET amplification correlated with adverse pathological characteristics and poor prognosis.IHC was a poor screening tool for MET amplification. 展开更多
关键词 Colorectal cancer MET amplification Pathological features Prognosis Fluorescence in situ hybridization
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O(logN) Algorithm for Amplitude Amplification and O(logN) Algorithms for Amplitude Transfer in Grover’s Algorithm
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作者 Ying Liu 《American Journal of Computational Mathematics》 2024年第2期169-188,共20页
Grovers algorithm is a category of quantum algorithms that can be applied to many problems through the exploitation of quantum parallelism. The Amplitude Amplification in Grovers algorithm is T = O(N). This paper intr... Grovers algorithm is a category of quantum algorithms that can be applied to many problems through the exploitation of quantum parallelism. The Amplitude Amplification in Grovers algorithm is T = O(N). This paper introduces two new algorithms for Amplitude Amplification in Grovers algorithm with a time complexity of T = O(logN), aiming to improve efficiency in quantum computing. The difference between Grovers algorithm and our first algorithm is that the Amplitude Amplification ratio in Grovers algorithm is an arithmetic series and ours, a geometric one. Because our Amplitude Amplification ratios converge much faster, the time complexity is improved significantly. In our second algorithm, we introduced a new concept, Amplitude Transfer where the marked state is transferred to a new set of qubits such that the new qubit state is an eigenstate of measurable variables. When the new qubit quantum state is measured, with high probability, the correct solution will be obtained. 展开更多
关键词 Quantum Computing ORACLE Amplitude amplification Grover’s Algorithm
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Study on Characteristics of a High-Precision Cold Gas Micro Thruster
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作者 Zhaoli Wang Changbin Guan +2 位作者 Xudong Wang Weijie Zheng Longfei Su 《Engineering(科研)》 2024年第1期38-45,共8页
In order to improve the reliability of the spacecraft micro cold gas propulsion system and realize the precise control of the spacecraft attitude and orbit, a micro-thrust, high-precision cold gas thruster is carried ... In order to improve the reliability of the spacecraft micro cold gas propulsion system and realize the precise control of the spacecraft attitude and orbit, a micro-thrust, high-precision cold gas thruster is carried out, at the same time due to the design requirements of the spacecraft, this micro-thrust should be continuous working more than 60 minutes, the traditional solenoid valve used for the thrusts can’t complete the mission, so a long-life micro latching valve is developed as the control valve for this micro thruster, because the micro latching valve can keep its position when it cuts off the outage. Firstly, the authors introduced the design scheme and idea of the thruster. Secondly, the performance of the latching valve and the flow characteristics of the nozzle were simulated. Finally, from the experimental results and compared with the numerical study, it shows that the long-life micro cold gas thruster developed in this paper meets the mission requirements. 展开更多
关键词 high-precision Micro Thruster Performance Flow Characteristic EXPERIMENT
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Research and Application of"Soft"Devices for Realizing Servo Amplification
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作者 Shuwen Zhang Fei Meng 《Frontiers of Metallurgical Industry》 2024年第1期31-33,共3页
This article analyzes and discusses the working principle and problems encountered by various servo amplification devices used in the on-site continuous adjustment system,analyzes and discusses the application of the ... This article analyzes and discusses the working principle and problems encountered by various servo amplification devices used in the on-site continuous adjustment system,analyzes and discusses the application of the servo mechanism,and analyzes the mechanism of the servo device's implementation of the"positioning"func-tion on the control device.Intended to guide the continuous adjustment process in controlling the function/accuracy of actuator equipment and application debugging,ensuring the safe and stable operation of production equipment and facilities. 展开更多
关键词 servo amplification working mechanism APPLICATION
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Current trends in nanomaterials-mediated biosensing platforms and signal amplification strategies for antibiotics detection in dairy products
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作者 Cui-Yun Zhou Feng Jiang Chen-Xi Huang 《Food and Health》 2024年第1期28-42,共15页
Dairy products have become one of the most prevalent daily foods worldwide,but safety concerns are rising.In dairy farming,unscrupulous traders misuse antibiotics to treat some diseases such as mastitis in cows,leadin... Dairy products have become one of the most prevalent daily foods worldwide,but safety concerns are rising.In dairy farming,unscrupulous traders misuse antibiotics to treat some diseases such as mastitis in cows,leading to antibiotic residues in dairy products.Rapid,sensitive,and simple detection methods for antibiotic residues are particularly important for food safety in dairy products.Traditional detection technology can effectively detect antibiotics,but there are defects such as complicated pre-treatment and high cost.Biosensors are widely used in food safety due to fast detection speed,low detection cost,strong anti-interference ability,and suitability for the field application.Nevertheless,these sensors often fail to trigger the signal conversion output due to low target concentration.To cope with this issue,some high-efficiency signal amplification systems can be introduced to improve the detection sensitivity and linear range of biosensors.In this review,we focused on:(i)Sources and toxicity of major antibiotics in animal-derived foods.(ii)Nanomaterial-mediated biosensors for real-time detection of target antibiotics in animal-derived foods.(iii)Signal amplification techniques to increase the sensitivity of biosensors.Finally,future prospects and challenges in this research field are discussed. 展开更多
关键词 Nanosensors Signal amplification Antibiotics detection Animal-derived foods.
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Assessment of future Antarctic amplification of surface temperature change under different Scenarios from CMIP6 被引量:2
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作者 ZHU Jiang-ping XIE Ai-hong +1 位作者 QIN Xiang XU Bing 《Journal of Mountain Science》 SCIE CSCD 2023年第4期1074-1089,共16页
Global warming may result in increased polar amplification,but future temperature changes under different climate change scenarios have not been systematically investigated over Antarctica.An index of Antarctic amplif... Global warming may result in increased polar amplification,but future temperature changes under different climate change scenarios have not been systematically investigated over Antarctica.An index of Antarctic amplification(AnA)is defined,and the annual and seasonal variations of Antarctic mean temperature are examined from projections of the Coupled Model Intercomparison Project Phase 6(CMIP6)under scenarios SSP119,SSP126,SSP245,SSP370 and SSP585.AnA occurs under all scenarios,and is strongest in the austral summer and autumn,with an AnA index greater than 1.40.Although the warming over Antarctica accelerates with increased anthropogenic forcing,the magnitude of AnA is greatest in SSP126 instead of in SSP585,which may be affected by strong ocean heat uptake in high forcing scenario.Moreover,future AnA shows seasonal difference and regional difference.AnA is most conspicuous in the East Antarctic sector,with the amplification occurring under all scenarios and in all seasons,especially in austral summer when the AnA index is greater than 1.50,and the weakest signal appears in austral winter.Differently,the AnA over West Antarctica is strongest in austral autumn.Under SSP585,the temperature increase over the Antarctic Peninsula exceeds 0.5℃when the global average warming increases from 1.5℃to 2.0℃above preindustrial levels,except in the austral summer,and the AnA index in this region is strong in the austral autumn and winter.The projections suggest that the warming rate under different scenarios might make a large difference to the future AnA. 展开更多
关键词 Antarctic amplification amplification index CMIP6 ScenarioMIP Near-surface temperature Climate change
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Research progress and prospects of nucleic acid isothermal amplification technology 被引量:1
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作者 SHUHUI WU PING XU +1 位作者 XIANGBIN XU SONG-BAI LIU 《BIOCELL》 SCIE 2023年第11期2385-2395,共11页
Nucleic acid(DNA and RNA)detection and quantification methods play vital roles in molecular biology.With the development of molecular biology,isothermal amplification of DNA/RNA,as a new molecular biology technology,c... Nucleic acid(DNA and RNA)detection and quantification methods play vital roles in molecular biology.With the development of molecular biology,isothermal amplification of DNA/RNA,as a new molecular biology technology,can be amplified under isothermal condition,it has the advantages of high sensitivity,high specificity,and high efficiency,and has been applied in various fields of biotechnology,including disease diagnosis,pathogen detection,food hygiene and safety detection and so on.This paper introduces the progress of isothermal amplification technology,including rolling circle amplification(RCA),nucleic acid sequence-dependent amplification(NASBA),strand displacement amplification(SDA),loop-mediated isothermal amplification(LAMP),helicase-dependent amplification(HDA),recombinase polymerase amplification(RPA),cross-priming amplification(CPA),and its principle,advantages and disadvantages,and application development are briefly summarized. 展开更多
关键词 Isothermal amplification Rolling circle amplification Nucleic acid sequence-based amplification Strand displacement amplification Loop-mediated isothermal amplification Helicase-dependent amplification Recombinase polymerase amplification Cross-primer amplification
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Moho depth inversion in the Tibetan Plateau from high-precision gravity data 被引量:4
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作者 HuiYou He Jian Fang +3 位作者 HePing Sun DongMei Guo ZhiXin Xue Jing Hou 《Earth and Planetary Physics》 CAS CSCD 2023年第4期487-498,共12页
The Tibetan Plateau(TP)is the youngest orogenic belt resulting from a continental collision on the Earth.It is a natural laboratory for studying continental dynamics,such as continental convergence,plate subduction,an... The Tibetan Plateau(TP)is the youngest orogenic belt resulting from a continental collision on the Earth.It is a natural laboratory for studying continental dynamics,such as continental convergence,plate subduction,and plateau uplift.Investigating the deep structure of the TP has always been a popular issue in geological research.The Moho is the boundary between the crust and the mantle and therefore plays a crucial role in the Earth’s structure.Parameters such as depth and lateral variation,as well as the fine structure of the crust-mantle interface,reveal the lithospheric dynamics in the TP.Two methods are generally employed to study the Moho surface:seismic detection and gravity inversion.Seismic detection has the characteristic of high precision,but it is limited to a few cross-sectional lines and is quite costly.It is not suitable for and cannot be carried out over a large area of the TP.The Moho depth over a large area can be obtained through gravity inversion,but this method is affected by the nature of gravity data,and the accuracy of the inversion method is lower than that of seismic detection.In this work,a high-precision gravity field model was selected.The Parker-Oldenburg interface inversion method was used,within the constraints of seismic observations,and the Bott iteration method was introduced to enhance the inversion efficiency.The Moho depth in the TP was obtained with high precision,consistent with the seismic detection results.The research results showed that the shape of the Moho in the TP is complex and the variation range is large,reaching 60−80 km.In contrast with the adjacent area,a clear zone of sharp variation appears at the edge of the plateau.In the interior of the TP,the buried depth of the Moho is characterized by two depressions and two uplifts.To the south of the Yarlung Zangbo River,the Moho inclines to the north,and to the north,the Moho depresses downward,which was interpreted as the Indian plate subducting to the north below Tibet.The Moho depression on the north side of the Qiangtang block,reaching 72 km deep,may be a result of the southward subduction of the lithosphere.The Moho uplift of the Qiangtang block has the same strike as the Bangong−Nujiang suture zone,which may indicate that the area is compensated by a low-density and low-velocity mantle. 展开更多
关键词 GRAVITY MOHO Tibetan Plateau SGG-UGM-2(2159-order high-precision gravity field model)
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High-precision nonisothermal transient wellbore drift flow model suitable for the full flow pattern domain and full dip range 被引量:1
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作者 Wen-Qiang Lou Da-Lin Sun +5 位作者 Xiao-Hui Sun Peng-Fei Li Ya-Xin Liu Li-Chen Guan Bao-Jiang Sun Zhi-Yuan Wang 《Petroleum Science》 SCIE EI CAS CSCD 2023年第1期424-446,共23页
A reliable multiphase flow simulator is an important tool to improve wellbore integrity and production decision-making.To develop a multiphase flow model with high adaptability and high accuracy,we first build a multi... A reliable multiphase flow simulator is an important tool to improve wellbore integrity and production decision-making.To develop a multiphase flow model with high adaptability and high accuracy,we first build a multiphase flow database with 3561 groups of data and developed a drift closure relationship with stable continuity and high adaptability.Second,a high-order numerical scheme with strong fault capture ability is constructed by effectively combining MUSCL technology,van Albada slope limiter and AUSMV numerical scheme.Finally,the energy equation is coupled into the AUSMV numerical scheme of the drift flow model in the form of finite difference.A transient non-isothermal wellbore multiphase flow model with wide applicability is formed by integrating the three technologies,and the effects of various factors on the calculation accuracy are studied.The accuracy of the simulator is verified by comparing the measurement results with the blowout experiment of a full-scale experimental well. 展开更多
关键词 Drift closure relation Non-isothermal model high-precision Multiphase flow solver Wellbore pressure control
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Influence of Surface Types on the Seasonality and Inter-Model Spread of Arctic Amplification in CMIP6 被引量:1
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作者 Yanchi LIU Yunqi KONG +1 位作者 Qinghua YANG Xiaoming HU 《Advances in Atmospheric Sciences》 SCIE CAS CSCD 2023年第12期2288-2301,共14页
A robust phenomenon termed the Arctic Amplification(AA)refers to the stronger warming taking place over the Arctic compared to the global mean.The AA can be confirmed through observations and reproduced in climate mod... A robust phenomenon termed the Arctic Amplification(AA)refers to the stronger warming taking place over the Arctic compared to the global mean.The AA can be confirmed through observations and reproduced in climate model simulations and shows significant seasonality and inter-model spread.This study focuses on the influence of surface type on the seasonality of AA and its inter-model spread by dividing the Arctic region into four surface types:ice-covered,ice-retreat,ice-free,and land.The magnitude and inter-model spread of Arctic surface warming are calculated from the difference between the abrupt-4×CO_(2)and pre-industrial experiments of 17 CMIP6 models.The change of effective thermal inertia(ETI)in response to the quadrupling of CO_(2) forcing is the leading mechanism for the seasonal energy transfer mechanism,which acts to store heat temporarily in summer and then release it in winter.The ETI change is strongest over the ice-retreat region,which is also responsible for the strongest AA among the four surface types.The lack of ETI change explains the nearly uniform warming pattern across seasons over the ice-free(ocean)region.Compared to other regions,the ice-covered region shows the maximum inter-model spread in JFM,resulting from a stronger inter-model spread in the oceanic heat storage term.However,the weaker upward surface turbulent sensible and latent heat fluxes tend to suppress the inter-model spread.The relatively small inter-model spread during summer is caused by the cancellation of the inter-model spread in ice-albedo feedback with that in the oceanic heat storage term. 展开更多
关键词 Arctic amplification surface type dependence seasonal energy transfer effective thermal inertia
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Nonlinear optical imaging by detection with optical parametric amplification(invited paper) 被引量:1
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作者 Yi Sun Haohua Tu Stephen A.Boppart 《Journal of Innovative Optical Health Sciences》 SCIE EI CAS CSCD 2023年第1期5-14,共10页
Nonlinear optical imaging is a versatile tool that has been proven to be exceptionally useful in various research fields.However,due to the use of photomultiplier tubes(PMTs),the wide application of nonlinear optical ... Nonlinear optical imaging is a versatile tool that has been proven to be exceptionally useful in various research fields.However,due to the use of photomultiplier tubes(PMTs),the wide application of nonlinear optical imaging is limited by the incapability of imaging under am-bient light.In this paper,we propose and demonstrate a new optical imaging detection method based on optical parametric amplification(OPA).As a nonlinear optical process,OPA in-trinsically rejects ambient light photons by coherence gating.Periodical poled lithium niobate(PPLN)crystals are used in this study as the media for OPA.Compared to bulk nonlinear optical crystals,PPLN crystals support the generation of OPA signal with lower pump power.Therefore,this characteristic of PPLN crystals is particularly beneficial when using high-repetition-rate lasers,which facilitate high-speed optical signal detection,such as in spec-troscopy and imaging.A PPLN-based OPA system was built to amplify the emitted imaging signal from second harmonic generation(SHG)and coherent anti-Stokes Raman scattering(CARS)microscopy imaging,and the amplified optical signal was strong enough to be detected by a biased photodiode under ordinary room light conditions.With OPA detection,ambient-light-on SHG and CARS imaging becomes possible,and achieves a similar result as PMT detection under strictly dark environments.These results demonstrate that OPA can be used as a substitute for PMTs in nonlinear optical imaging to adapt it to various applications with complex.light ing conditions. 展开更多
关键词 Nonlinear optical microscopy optical parametric amplification optical detection
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Personal glucose meters coupled with signal amplification technologies for quantitative detection of non-glucose targets:Recent progress and challenges in food safety hazards analysis 被引量:1
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作者 Feng He Haijie Wang +7 位作者 Pengfei Du Tengfei Li Weiting Wang Tianyu Tan Yaobo Liu Yanli Ma Yuanshang Wang A.M.Abd El-Aty 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2023年第3期223-238,共16页
Ensuring food safety is paramount worldwide.Developing effective detection methods to ensure food safety can be challenging owing to trace hazards,long detection time,and resource-poor sites,in addition to the matrix ... Ensuring food safety is paramount worldwide.Developing effective detection methods to ensure food safety can be challenging owing to trace hazards,long detection time,and resource-poor sites,in addition to the matrix effects of food.Personal glucose meter(PGM),a classic point-of-care testing device,possesses unique application advantages,demonstrating promise in food safety.Currently,many studies have used PGM-based biosensors and signal amplification technologies to achieve sensitive and specific detection of food hazards.Signal amplification technologies have the potential to greatly improve the analytical performance and integration of PGMs with biosensors,which is crucial for solving the challenges associated with the use of PGMs for food safety analysis.This review introduces the basic detection principle of a PGM-based sensing strategy,which consists of three key factors:target recognition,signal transduction,and signal output.Representative studies of existing PGM-based sensing strategies combined with various signal amplification technologies(nanomaterial-loaded multienzyme labeling,nucleic acid reaction,DNAzyme catalysis,responsive nanomaterial encapsulation,and others)in the field of food safety detection are reviewed.Future perspectives and potential opportunities and challenges associated with PGMs in the field of food safety are discussed.Despite the need for complex sample preparation and the lack of standardization in the field,using PGMs in combination with signal amplification technology shows promise as a rapid and cost-effective method for food safety hazard analysis. 展开更多
关键词 Food safety Personal glucose meter Signal amplification Point-of-care testing Detection principle
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Ultrathin metal-organic framework nanosheets (Cu-TCPP)-based isothermal nucleic acid amplification for food allergen detection 被引量:1
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作者 Jiale Gao Xiaodong Sun +3 位作者 Yongxin Liu Bing Niu Qin Chen Xueen Fang 《Food Science and Human Wellness》 SCIE CSCD 2023年第5期1788-1798,共11页
The rapid and accurate detection of peanuts and soybeans allergen is important to the food safety. In this study, Cu-TCPP nanosheet, a kind of ultra-thin metal-organic framework(MOF)was synthesized and applied in loop... The rapid and accurate detection of peanuts and soybeans allergen is important to the food safety. In this study, Cu-TCPP nanosheet, a kind of ultra-thin metal-organic framework(MOF)was synthesized and applied in loop-mediated isothermal amplification(named Cu-TCPP@LAMP), which can inhibit the non-specific amplification by absorbing and precise temperature releasing of single primer. As thus, Cu-TCPP@LAMP can achieve high sensitivity and specific amplification of the target gene. As a result, peanut and soybean allergens genes contained in food were successfully detected with a favorable detection sensitivity(5 ng/μL for peanuts and 10 ng/μL for soybeans)and reliable repeatability(The coefficient of variation was 3.38% for peanuts and 3.33% for soybeans). Moreover, the established method was utilized for detection of several commercial products, and had a high consistency with the standard method. Apart from food allergens, this novel assay can be widely used in other areas, such as pathogen detection, tumor nucleic acid detection and so on. 展开更多
关键词 Cu-TCPP nanosheet Plant allergen Nucleic acid detection Loop-mediated isothermal amplification
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Enhanced phase sensitive amplification towards improving noise immunity
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作者 郭辉 李治 +2 位作者 孙恒信 刘奎 郜江瑞 《Chinese Physics B》 SCIE EI CAS CSCD 2023年第5期126-130,共5页
Quantum states are essential resource for quantum-enhanced applications.Loss incurred in the distribution channel,however,dissipates the high signal-to-noise ratio advantage enjoyed by the squeezed state.Here,we first... Quantum states are essential resource for quantum-enhanced applications.Loss incurred in the distribution channel,however,dissipates the high signal-to-noise ratio advantage enjoyed by the squeezed state.Here,we first demonstrate noise immunity enhancement by using phase-sensitive amplifier(PSA)with measurement-based noiseless linear amplifier(MB-NLA).We explore the signal transfer capability with the amplifier in a noisy channel.The MB-NLA enhanced PSA has obvious suppression effect on channel noises,especially it has improvement for the noise contaminated signal.Better performance can be achieved by flexibly adjusting amplifier parameters.With the amplifier,it is promising to overcome the entanglement-distribution loss and show its superiority in squeezing based quantum sensing. 展开更多
关键词 phase sensitive amplification noiseless linear amplification quantum sensing
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Influence of coupling asymmetry on signal amplification in a three-node motif
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作者 梁晓明 方超 +1 位作者 张希昀 吕华平 《Chinese Physics B》 SCIE EI CAS CSCD 2023年第1期239-243,共5页
The three-node feedforward motif has been revealed to function as a weak signal amplifier. In this motif, two nodes(input nodes) receive a weak input signal and send it unidirectionally to the third node(output node).... The three-node feedforward motif has been revealed to function as a weak signal amplifier. In this motif, two nodes(input nodes) receive a weak input signal and send it unidirectionally to the third node(output node). Here, we change the motif's unidirectional couplings(feedforward) to bidirectional couplings(feedforward and feedback working together).We find that a small asymmetric coupling, in which the feedforward effect is stronger than the feedback effect, may enable the three-node motif to go through two distinct dynamic transitions, giving rise to a double resonant signal response. We present an analytical description of the double resonance, which agrees with the numerical findings. 展开更多
关键词 network motif SYNCHRONIZATION coupling asymmetry signal amplification
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Development and Characterization of Microsatellite Markers for Harpadon nehereus Based on High-Throughput Sequencing and Cross-Species Amplification in Three Myctophiformes Fishes
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作者 HUANG Xinxin NING Zijun YANG Tianyan 《Journal of Ocean University of China》 SCIE CAS CSCD 2023年第1期181-188,共8页
Harpadon nehereus is a widespread economical fish found in the coastal seas of China and has important ecological value in the marine ecosystem.H_(o)wever,its germplasm resources have been seriously degraded due to na... Harpadon nehereus is a widespread economical fish found in the coastal seas of China and has important ecological value in the marine ecosystem.H_(o)wever,its germplasm resources have been seriously degraded due to natural factors and anthropogenic activities.In this study,high-throughput sequencing was applied to search for microsatellite loci in H.nehereus transcriptome to provide references for its resource conservation and utilization.Polymorphic loci were developed by non-denaturing polyacrylamide gel electrophoresis,and their cross-species amplified ability was detected in three related species.A total of 5652 microsatellites were identified from 16974320 unigenes.Among the primer pairs designed for 100 SSRs for PCR amplification,80%were successfully amplified,and 26 loci were polymorphic with a high number of alleles from 3 to 11 each.The expected(H_(e))and observed(H_(o))heterozygosities were 0.355–0.885 and 0.375–0.958,respectively.Most of the loci were highly polymorphic(polymorphism information content:0.316–0.852;mean:0.713),and these markers can be applied in the population genetic diversity research of H.nehereus.H_(o)wever,the transferability of these primers was low,probably because of the close relation of the collected species.In follow-up work,simple sequence repeats will be excavated with genome-based technologies,and related species will be gathered to address the present inadequacies. 展开更多
关键词 Harpadon nehereus microsatellite markers high-throughput sequencing cross-species amplification
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Dissipation and amplification management in an electrical model of microtubules: Hybrid behavior network
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作者 Sedric Ndoungalah Guy Roger Deffo +1 位作者 Arnaud Djine Serge Bruno Yamgoue 《Chinese Physics B》 SCIE EI CAS CSCD 2023年第11期392-400,共9页
The control of dissipation and amplification of solitary waves in an electrical model of a microtubule is demonstrated.This model consists of a shunt nonlinear resistance–capacitance(J(V)–C(V)) circuit and a series ... The control of dissipation and amplification of solitary waves in an electrical model of a microtubule is demonstrated.This model consists of a shunt nonlinear resistance–capacitance(J(V)–C(V)) circuit and a series resistance–inductance(R–L) circuit. Through linear dispersion analysis, two features of the network are found, that is, low bandpass and bandpass filter characteristics. The effects of the conductance’s parameter λ on the linear dispersion curve are also analyzed. It appears that an increase of λ induces a decrease(an increase) of the width of the bandpass filter for positive(negative) values of λ. By applying the reductive perturbation method, we derive the equation governing the dynamics of the modulated waves in the system. This equation is the well-known nonlinear Schr?dinger equation extended by a linear term proportional to a hybrid parameter σ, i.e., a dissipation or amplification coefficient. Based on this parameter, we successfully demonstrate the hybrid behavior(dissipation and amplification) of the system. The exact and approximate solitary wave solutions of the obtained equation are derived, and the effects of the coefficient σ on the characteristic parameters of these waves are investigated. Using the analytical solutions found, we show numerically that the waves that are propagated throughout the system can be dissipated, amplified, or remain stable depending on the network parameters. These results are not only in agreement with the analytical predictions, but also with the existing experimental results in the literature. 展开更多
关键词 MICROTUBULE dissipation and amplification hybrid behavior solitary wave solutions
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