High-temperature stress suppresses allene oxide cyclase 2 and causes male sterility in cotton by disrupting jasmonic acid signaling

Cotton(Gossypium spp.) yield is reduced by stress. In this study, high temperature(HT) suppressed the expression of the jasmonic acid(JA) biosynthesis gene allene oxide cyclase 2(GhAOC2), reducing JA content and causing male sterility in the cotton HT-sensitive line H05. Anther sterility was reversed by exogenous application of methyl jasmonate(MeJA) to early buds. To elucidate the role of GhAOC2 in JA biosynthesis and identify its putative contribution to the anther response to HT, we created gene knockout cotton plants using the CRISPR/Cas9 system. Ghaoc2 mutant lines showed male-sterile flowers with reduced JA content in the anthers at the tetrad stage(TS), tapetum degradation stage(TDS), and anther dehiscence stage(ADS). Exogenous application of MeJA to early mutant buds(containing TS or TDS anthers) rescued the sterile pollen and indehiscent anther phenotypes, while ROS signals were reduced in ADS anthers. We propose that HT downregulates the expression of GhAOC2 in anthers, reducing JA biosynthesis and causing excessive ROS accumulation in anthers, leading to male sterility. These findings suggest exogenous JA application as a strategy for increasing male fertility in cotton under HT.

Osmotic Regulation,Antioxidant Enzyme Activities and Photosynthetic Characteristics of Tree Peony(Paeonia suffruticosa Andr.)in Response to High-Temperature Stress

Tree peony(Paeonia suffruticosa Andr.)is a traditional Chinese flower,which prefers cool weather.However,high temperature in summer in the middle and lower reaches of the Yangtze River restricts its growth and development.In this study,osmotic regulation,antioxidant enzyme activities,and photosynthetic characteristics of tree peony in response to high-temperature stress were investigated.The results showed that high-temperature stress had destroyed the cell membrane,manifested as the increased relative electrical conductivity and malondialdehyde content.Moreover,high-temperature stress led to excessive accumulation of reactive oxygen species,thereby,activating antioxidant enzyme activities.Also,photosynthetic parameters and chlorophyll fluorescence parameters directly reflected the damage to the photosystem II reflection center under high-temperature stress.In addition,high-temperature stress led to stomatal closure and chloroplast damage.This study revealed the physiological responses of tree peony to high-temperature stress,laying a foundation for the promotion of tree peony in high-temperature areas and the improvement of high-temperature resistance.

Physiological Response of Spring Soybean Seedlings Under High-temperature Stress

To understand the response characteristics of soybean seedling resistance systems to high-temperature stress,the spring soybean varieties HN44 and HN65 were selected in this study as the experimental materials,as they had substantial differences in stress resistance.Soybean physiological status was studied under high-temperature stress.Sand culture was used in the experiment,and soybeans at the seedling stage were treated with high-temperature stress.The results showed that the activity of antioxidases and osmotic regulatory substance contents in soybean increased under high-temperature treatment for a certain period of time,the activity of antioxidases and osmotic regulatory substance contents decreased after continuous treatment,and high temperatures continuously increased malondialdehyde contents in the two varieties.At high temperatures,the antioxidant activity and osmotic regulator contents of HN44 were higher than those of HN65,and the malondialdehyde contents of HN44 were higher than those of HN65.The above results showed that HN44 soybeans exhibited significantly higher resistance to high-temperature stress than HN65 variety.

The impact of high-temperature stress on rice: Challenges and solutions

Heat stress (HS) caused by rapidly warming climate has become a serious threat to global food security.Rice (Oryza sativa L.) is a staple food crop for over half of the world’s population,and its yield and quality are often reduced by HS.There is an urgent need for breeding heat-tolerant rice cultivars.Rice plants show various morphological and physiological symptoms under HS.Precise analysis of the symptoms(phenotyping) is essential for the selection of elite germplasm and the identification of thermotolerance genes.In response to HS,rice plants trigger a cascade of events and activate complex transcriptional regulatory networks.Protein homeostasis under HS is especially important for rice thermotolerance,which is affected by protein quality control,effective elimination of toxic proteins,and translational regulation.Although some agronomic and genetic approaches for improving heat tolerance have been adopted in rice,the molecular mechanisms underlying rice response to HS are still elusive,and success in engineering rice thermotolerance in breeding has been limited.In this review,we summarize HS-caused symptoms in rice and progress in heat-stress sensing and signal cascade research,and propose approaches for improving rice thermotolerance in future.

Modeling the effects of extreme high-temperature stress at anthesis and grain filling on grain protein in winter wheat

Extreme high-temperature stress(HTS) associated with climate change poses potential threats to wheat grain yield and quality. Wheat grain protein concentration(GPC) is a determinant of wheat quality for human nutrition and is often neglected in attempts to assess climate change impacts on wheat production. Crop models are useful tools for quantification of temperature impacts on grain yield and quality.Current crop models either cannot simulate or can simulate only partially the effects of HTS on crop N dynamics and grain N accumulation. There is a paucity of observational data on crop N and grain quality collected under systematic HTS scenarios to develop algorithms for model improvement as well as evaluate crop models. Two-year phytotron experiments were conducted with two wheat cultivars under HTS at anthesis, grain filling, and both stages. HTS significantly reduced total aboveground N and increased the rate of grain N accumulation, while total aboveground N and the rate of grain N accumulation were more sensitive to HTS at anthesis than at grain filling. The observed relationships between total aboveground N, rate of grain N accumulation, and HTS were quantified and incorporated into the WheatGrow model. The new HTS routines improved simulation of the dynamics of total aboveground N, grain N accumulation, and GPC by the model. The improved model provided better estimates of total aboveground N, grain N accumulation, and GPC under HTS(the normalized root mean square error was reduced by 40%, 85%, and 80%, respectively) than the original WheatGrow model. The improvements in the model enhance its applicability to the assessment of climate change effects on wheat grain quality by reducing the uncertainties of simulating N dynamics and grain quality under HTS.

Harness High-Temperature Thermal Energy via Elastic Thermoelectric Aerogels

Despite notable progress in thermoelectric(TE)materials and devices,developing TE aerogels with high-temperature resistance,superior TE performance and excellent elasticity to enable self-powered high-temperature monitoring/warning in industrial and wearable applications remains a great challenge.Herein,a highly elastic,flame-retardant and high-temperature-resistant TE aerogel,made of poly(3,4-ethylene dioxythiophene):poly(styrenesulfonate)/single-walled carbon nanotube(PEDOT:PSS/SWCNT)composites,has been fabricated,displaying attractive compression-induced power factor enhancement.The as-fabricated sensors with the aerogel can achieve accurately pressure stimuli detection and wide temperature range monitoring.Subsequently,a flexible TE generator is assembled,consisting of 25 aerogels connected in series,capable of delivering a maximum output power of 400μW when subjected to a temperature difference of 300 K.This demonstrates its outstanding high-temperature heat harvesting capability and promising application prospects for real-time temperature monitoring on industrial high-temperature pipelines.Moreover,the designed self-powered wearable sensing glove can realize precise wide-range temperature detection,high-temperature warning and accurate recognition of human hand gestures.The aerogel-based intelligent wearable sensing system developed for firefighters demonstrates the desired self-powered and highly sensitive high-temperature fire warning capability.Benefitting from these desirable properties,the elastic and high-temperature-resistant aerogels present various promising applications including self-powered high-temperature monitoring,industrial overheat warning,waste heat energy recycling and even wearable healthcare.

Synergistic effect of Zr and Mo on precipitation and high-temperature properties of Al-Si-Cu-Mg alloys

This study focuses on finding a solution to the sharp decline in mechanical properties of Al-Si-Cu-Mg alloys due to rapid coarsening of traditional intermediate phases at high temperature.A new type of modified al oy,to be used in automobile engines at high temperatures,was prepared by adding Zr and Mo into Al-Si-Cu-Mg alloy.The synergistic effects of Zr and Mo on the microstructure evolution and high-temperature mechanical properties were studied.Results show that the addition of Zr and Mo generates a series of intermetallic phases dispersed in the alloy.They can improve the strength of the alloy by hindering dislocation movement and crack propagation.In addition,some nano-strengthened phases show coherent interfaces with the matrix and improve grain refinement.The addition of Mo greatly improves the heat resistance of the alloy.The extremely low diffusivity of Mo enables it to improve the thermal stability of the intermetallic phases,inhibit precipitation during aging,reduce the size of the precipitates,and improve the heat resistance of the alloy.

HZSM-5 zeolites undergoing the high-temperature process for boosting the bimolecular reaction in n-heptane catalytic cracking

High-temperature treatment is key to the preparation of zeolite catalysts.Herein,the effects of hightemperature treatment on the property and performance of HZSM-5 zeolites were studied in this work.X-Ray diffraction,N2physisorption,27Al magic angle spinning nuclear magnetic resonance(MAS NMR),and temperature-programmed desorption of ammonia results indicated that the hightemperature treatment at 650℃ hardly affected the inherent crystal and texture of HZSM-5zeolites but facilitated the conversion of framework Al to extra-framework Al,reducing the acid site and enhancing the acid strength.Moreover,the high-temperature treatment improved the performance of HZSM-5 zeolites in n-heptane catalytic cracking,promoting the conversion and light olefins yield while inhibiting coke formation.Based on the kinetic and mechanism analysis,the improvement of HZSM-5 performance caused by high-temperature treatment has been attributed to the formation of extra-framework Al,which enhanced the acid strength,facilitated the bimolecular reaction,and promoted the entropy change to overcome a higher energy barrier in n-heptane catalytic cracking.

Novel Method for Evaluating the Aging of Aviation Turbine Engine Oils via High-Temperature Bearing Deposit Tests

Aviation turbine engine oils require excellent thermal-oxidative stability because of their high-temperature environments.High-temperature bearing deposit testing is a mandatory method for measuring the thermal-oxidative performance of aviation lubricant oils,and the relevant apparatus was improved in the present study.Two different commercial aviation turbine engine oils were tested,one with standard performance(known as the SL oil)and the other with high thermal stability,and their thermal-oxidative stability characteristics were evaluated.After 100 h of high-temperature bearing testing,the SL oil was analyzed by using various analytical techniques to investigate its thermal-oxidative process in the bearing test,with its thermal-oxidative degradation mechanism also being discussed.The results indicate that the developed high-temperature bearing apparatus easily meets the test requirements of method 3410.1 in standard FED-STD-791D.The viscosity and total acid number(TAN)of the SL oil increased with the bearing test time,and various deposits were produced in the bearing test,with the micro-particles of the carbon deposits being sphere-like,rod-like,and sheet-like in appearance.The antioxidant additives in the oil were consumed very rapidly in the first 30 h of the bearing test,with N-phenyl-1-naphthylamine being consumed faster than dioctyldiphenylamine.Overall,the oil thermal-oxidative process involves very complex physical and chemical mechanisms.

High-performance and robust high-temperature polymer electrolyte membranes with moderate microphase separation by implementation of terphenyl-based polymers

Acid loss and plasticization of phosphoric acid(PA)-doped high-temperature polymer electrolyte membranes(HT-PEMs)are critical limitations to their practical application in fuel cells.To overcome these barriers,poly(terphenyl piperidinium)s constructed from the m-and p-isomers of terphenyl were synthesized to regulate the microstructure of the membrane.Highly rigid p-terphenyl units prompt the formation of moderate PA aggregates,where the ion-pair interaction between piperidinium and biphosphate is reinforced,leading to a reduction in the plasticizing effect.As a result,there are trade-offs between the proton conductivity,mechanical strength,and PA retention of the membranes with varied m/p-isomer ratios.The designed PA-doped PTP-20m membrane exhibits superior ionic conductivity,good mechanical strength,and excellent PA retention over a wide range of temperature(80–160°C)as well as satisfactory resistance to harsh accelerated aging tests.As a result,the membrane presents a desirable combination of performance(1.462 W cm^(-2) under the H_(2)/O_(2)condition,which is 1.5 times higher than that of PBI-based membrane)and durability(300 h at 160°C and 0.2 A cm^(-2))in the fuel cell.The results of this study provide new insights that will guide molecular design from the perspective of microstructure to improve the performance and robustness of HT-PEMs.

Integrated multi-scale approach combining global homogenization and local refinement for multi-field analysis of high-temperature superconducting composite magnets

Second-generation high-temperature superconducting(HTS)conductors,specifically rare earth-barium-copper-oxide(REBCO)coated conductor(CC)tapes,are promising candidates for high-energy and high-field superconducting applications.With respect to epoxy-impregnated REBCO composite magnets that comprise multilayer components,the thermomechanical characteristics of each component differ considerably under extremely low temperatures and strong electromagnetic fields.Traditional numerical models include homogenized orthotropic models,which simplify overall field calculation but miss detailed multi-physics aspects,and full refinement(FR)ones that are thorough but computationally demanding.Herein,we propose an extended multi-scale approach for analyzing the multi-field characteristics of an epoxy-impregnated composite magnet assembled by HTS pancake coils.This approach combines a global homogenization(GH)scheme based on the homogenized electromagnetic T-A model,a method for solving Maxwell's equations for superconducting materials based on the current vector potential T and the magnetic field vector potential A,and a homogenized orthotropic thermoelastic model to assess the electromagnetic and thermoelastic properties at the macroscopic scale.We then identify“dangerous regions”at the macroscopic scale and obtain finer details using a local refinement(LR)scheme to capture the responses of each component material in the HTS composite tapes at the mesoscopic scale.The results of the present GH-LR multi-scale approach agree well with those of the FR scheme and the experimental data in the literature,indicating that the present approach is accurate and efficient.The proposed GH-LR multi-scale approach can serve as a valuable tool for evaluating the risk of failure in large-scale HTS composite magnets.

High-pressure and high-temperature sintering of pure cubic silicon carbide:A study on stress-strain and densification

Silicon carbide(SiC)is a high-performance structural ceramic material with excellent comprehensive properties,and is unmatched by metals and other structural materials.In this paper,raw SiC powder with an average grain size of 5μm was sintered by an isothermal-compression process at 5.0 GPa and 1500?C;the maximum hardness of the sintered samples was31.3 GPa.Subsequently,scanning electron microscopy was used to observe the microscopic morphology of the recovered SiC samples treated in a temperature and extended pressure range of 0-1500?C and 0-16.0 GPa,respectively.Defects and plastic deformation in the SiC grains were further analyzed by transmission electron microscopy.Further,high-pressure in situ synchrotron radiation x-ray diffraction was used to study the intergranular stress distribution and yield strength under non-hydrostatic compression.This study provides a new viewpoint for the sintering of pure phase micron-sized SiC particles.

Seasonal Prediction of Extreme High-Temperature Days in Southwestern China Based on the Physical Precursors

Extreme high temperatures frequently occur in southwestern China,significantly impacting the local ecological system and economic development.However,accurate prediction of extreme high-temperature days(EHDs)in this region is still an unresolved challenge.Based on the spatiotemporal characteristics of EHDs over China,a domain-averaged EHD index over southwestern China(SWC-EHDs)during April-May is defined.The simultaneous dynamic and thermodynamic fields associated with the increased SWC-EHDs are a local upper-level anticyclonic(high-pressure)anomaly and wavy geopotential height anomaly patterns over Eurasia.In tracing the origins of the lower boundary anomalies,two physically meaningful precursors are detected for SWC-EHDs.They are the tripolar SST change tendency from December-January to February-March in the northern Atlantic and the February-March mean snow depth in central Asia.Using these two selected predictors,a physics-based empirical model prediction was applied to the training period of 1961–2005 to obtain a skillful prediction of the EHDs index,attaining a correlation coefficient of 0.76 in the independent prediction period(2006–19),suggesting that 58%of the total SWC-EHDs variability is predictable.This study provides an estimate for the lower bound of the seasonal predictability of EHDs as well as for the hydrological drought over southwestern China.

Research on the impact of high-temperature aging on the thermal safety of lithium-ion batteries

Understanding the thermal safety evolution of lithium-ion batteries during high-temperature usage conditions bears significant implications for enhancing the safety management of aging batteries.This work investigates the thermal safety evolution mechanism of lithium-ion batteries during high-temperature aging.Similarities arise in the thermal safety evolution and degradation mechanisms for lithium-ion batteries undergoing cyclic aging and calendar aging.Employing multi-angle characterization analysis,the intricate mechanism governing the thermal safety evolution of lithium-ion batteries during high-temperature aging is clarified.Specifically,lithium plating serves as the pivotal factor contributing to the reduction in the self-heating initial temperature.Additionally,the crystal structure of the cathode induced by the dissolution of transition metals and the reductive gas generated during aging attacking the crystal structure of the cathode lead to a decrease in thermal runaway triggering temperature.Furthermore,the loss of active materials and active lithium during aging contributes to a decline in both the maximum temperature and the maximum temperature rise rate,ultimately indicating a decrease in the thermal hazards of aging batteries.

Study on the effect of thermal deformation on the liquid seal of high-temperature molten salt pump in molten salt reactor

The high-temperature molten salt pump is the core equipment in a molten salt reactor that drives the flow of the molten salt coolant.Rotor stability is key to the continuous and reliable operation of the molten salt pump,and the liquid seal at the wear ring can affect the dynamic characteristics of the rotor system.When the molten salt pump is operated in the hightemperature molten salt medium,thermal deformation of the submerged parts inevitably occurs,changing clearance between the stator and rotor,affecting the leakage and dynamic characteristics of the seal.In this study,the seal leakage,seal dynamic characteristics,and rotor system dynamic characteristics are simulated and analyzed using finite element simulation software based on two cases of considering the effect of seal thermal deformation effect or not.The results show a significant difference in the leakage characteristics and dynamic characteristics of the seal obtained by considering the effect of seal thermal deformation and neglecting the effect of thermal deformation.The leakage flow rate decreases,and the first-order critical speed of the seal-bearing-rotor system decrease after considering the seal’s thermal deformation.

Analysis on high-temperature oxidation and growth stress of iron-based alloy using phase field method

High-temperature oxidation is an important property to evaluate thermal protection materials. However, since oxidation is a complex process involving microstructure evolution, its quantitative analysis has always been a challenge. In this work, a phase field method （PFM） based on the thermodynamics theory is developed to simulate the oxidation behavior and oxidation induced growth stress. It involves microstructure evolution and solves the problem of quantitatively computational analysis for the oxidation behavior and growth stress. Employing this method, the diffusion process, oxidation performance, and stress evolution axe predicted for Fe-Cr-A1-Y alloys. The numerical results agree well with the experimental data. The linear relationship between the maximum growth stress and the environment oxygen concentration is found. PFM provides a powerful tool to investigate high-temperature oxidation in complex environments.

Transcriptome Analysis of High-Temperature Stress in Developing Barley Caryopses： Early Stress Responses and Effects on Storage Compound Biosynthesis

High-temperature stress, like any abiotic stress, impairs the physiology and development of plants, including the stages of seed setting and ripening. We used the Affymetrix 22K Barley1 GeneChip microarray to investigate the response of developing barley （Hordeum vulgare） seeds, termed caryopses, after 0.5, 3, and 6 h of heat stress exposure; 958 induced and 1122 repressed genes exhibited spatial and temporal expression patterns that provide a detailed insight into the caryopses＇ early heat stress responses. Down-regulation of genes related to storage compound biosynthesis and cell growth provides evidence for a rapid impairment of the caryopsis＇ development. Increased levels of sugars and amino acids were indicative for both production of compatible solutes and feedback-induced accumulation of substrates for storage compound biosynthesis. Metadata analysis identified embryo and endosperm as primary locations of heat stress responses, indicating a strong impact of short-term heat stress on central developmental functions of the caryopsis. A comparison with heat stress responses in Arabidopsis shoots and drought stress responses in barley caryopses identified both conserved and presumably heat- and caryopsis-specific stress-responsive genes. Summarized, our data provide an important basis for further investigation of gene functions in order to aid an improved heat tolerance and reduced losses of yield in barley as a model for cereal crops.

A 3–5μm broadband YBCO high-temperature superconducting photonic crystal

Photonic crystal structures have excellent optical properties,so they are widely studied in conventional optical materials.Recent research shows that high-temperature superconducting periodic structures have natural photonic crystal features and they are favourable candidates for single-photon detection.Considering that superconductors have completely different properties from conventional optical materials,we study the energy level diagram and mid-infrared 3μm–5μm transmission spectrum of two-dimensional superconducting photonic crystals in both superconducting and quenched states with the finite element method.The energy level diagram of the circular crystal column superconducting structure shows that the structure has a large band gap width in both states.At the same fill factor,the circular crystal column superconducting structure has a larger band gap width than the others structures.For lattice structures,the zero transmission point of the square lattice structure is robust to the incident angle and environmental temperature.Our research has guiding significance for the design of new material photonic crystals,photon modulation and detection.

Enhancement of the Mechanical Performance of SiCf/Phenolic Composites after High-temperature Pyrolysis Using ZrC/B_(4)C Particles

The composites were prepared by modifying silicon carbide fiber with particles of zirconium carbide(ZrC)and boron carbide(B_(4)C)and incorporating them into a phenolic resin matrix.The influence of ZrC and B_(4)C on the mechanical performance of SiCf/phenolic composites after high-temperature pyrolysis was studied through flexural performance test.The results show that the composite material has good thermal stability and high-temperature mechanical properties.After static ablation at 1400℃ for 15 minutes,the flexural strength of the composite material reaches 286 MPa,which is still 7.3%higher than at room temperature,indicating that the composite material still has good mechanical properties even after heat treatment at 1400℃.

The Research for Surface Properties of Steel Slag Powder and High-Temperature Rheological Properties of Asphalt Mortar

In order to evaluate the feasibility of steel slag powder as filler,the coating properties of steel slag and limestone aggregate were compared by water boiling test,the micro morphology difierences between steel slag powder and mineral powder(limestone powder)were compared by scanning electron microscope(SEM),and the high-temperature rheological properties of asphalt mortar with difierent ratio of filler quality to asphalt quality(F/A)and difierent substitution rates of mineral powder(S/F)were studied by dynamic shear rheological test.The results show that the surface microstructure of steel slag powder is more abundant than that of mineral powder,and the adhesion of steel slag to asphalt is better than that of limestone.At the same temperature,the lower the ratio of S/F is,the greater the rutting factor and complex modulus will be.In addition,the complex modulus and rutting factor of the asphalt mortar increase with the increase of F/A,and the filler type and F/A have a negligible efiect on the phase angle.