Objective To study the effects of environmental multi-media lead pollution on blood lead and sex hormone levels among lead exposed males engaged in E-waste dismantling, and the correlation between confounding factors ...Objective To study the effects of environmental multi-media lead pollution on blood lead and sex hormone levels among lead exposed males engaged in E-waste dismantling, and the correlation between confounding factors and sex hormone levels. Methods An E-waste dismantling area in Taizhou of Zhejiang Province was selected as the research site. One hundred and fifty two samples were collected from the groundwater, soil, rice, corn, chicken, and pork in the dismantling area. The effects of the multi-media lead pollution on the male blood lead and sex hormone levels of FSH, LH, and T, as well as the correlation with confounding factors, were studied. Results The blood lead concentrations in the males aged under 31, from 31 to 45 and from 46 to 60 were 98.55, 100.23, and 101.45 I~/L, respectively. Of all the environmental media lead exposures, the groundwater, rice and soil were main contributing factors to the lead accumulation in humans. FSH and LH levels increased with the age while the T levels decreased with the age instead. There was a significant correlation between the FSH and LH levels and wearing masks. Conclusion There was correlation between the FSH, LH, and T levels, and the mean values of lead concentrations in environmental media, and the sex hormone levels were correlated with the confounding factor of wearing masks.展开更多
Objective To identify the morphological characteristics of pituitary luteinizing hormone (LH) cells after exogenous gonadal hormone treatment Methods Effects of various doses of estrogen, progesterone and their...Objective To identify the morphological characteristics of pituitary luteinizing hormone (LH) cells after exogenous gonadal hormone treatment Methods Effects of various doses of estrogen, progesterone and their combination on morphological parameters, including the size and shape of pituitary LH cells, the size of endocellular vacuoles, were observed and measured by immuno histochemistry and computer image analysis. Results Different kinds of gonadal hormones could recover the magnified LH cells to the normal level in ovariectomized rats. However, their final effects on the gonadotrophin levels and the cellular morphological characters of the LH cells were different. The low dose of estrogen elicited abundant hormone stored in the LH cells to an easy released status with a lot of different size of vacuoles. On the contrary, the high dose of estrogen inhibited the storage of LH, and the LH cells were filled with secretory granules and few vacuoles. The progesterone could promote the storage of LH in an uneasy released status. The administration of estrogen progesterone combination not only inhibited the storage of LH, but also the release of LH. In this group, the LH cells containing a large amount of secretory granules and a few vacuoles showed a better uniform shape compared with those administrated with high dose of estrogen. Conclusion: Different kinds of gonadal hormones could reverse the excessive secretion of LH and recover the morphological change of LH cells to the normally physiological condition.展开更多
The effects of fluoride exposure on thefunctions of reproductive and endocrine systemshave attracted widespread attention in academiccircle nowadays. However, it is unclear whether thegene-environment interaction may ...The effects of fluoride exposure on thefunctions of reproductive and endocrine systemshave attracted widespread attention in academiccircle nowadays. However, it is unclear whether thegene-environment interaction may modify thesecretion and activity of hypothalamus-pituitary-ovarian （HPO） axis hormones. Thus, the aim of thisstudy was to explore the influence of fluorideexposure and follicle stimulating hormone receptor（FSHR） gene polymorphism on reproductivehormones in Chinese women. A cross sectionalstudy was conducted in seven villages of HenanProvince, China during 2010-2011. A total of 679women aged 18-48 years were recruited throughcluster sampling and divided into three groups, i.e.endemic fluorosis group （EFG）, defluoridationproject group （DFPG）, and control group （CG） basedon the local fluoride concentration in drinkingwater. The serum levels of gonadotropin releasinghormone （GnRH）, follicle stimulating hormone（FSH）, luteinizing hormone （LH）, and estradiol （E2）were determined respectively and the FSHRpolymorphism was detected by real time PCR assay.The results provided the preliminary evidenceindicating the gene-environment interaction onHPO axishormones in women.展开更多
This study was conducted to investigate the effect of different doses injection of human chorionic gonadotropin(HCG)hormone on fecundity and serum sex hormones(FSH,LH,estrogen(E2),progesterone(P4),testosterone(T))of A...This study was conducted to investigate the effect of different doses injection of human chorionic gonadotropin(HCG)hormone on fecundity and serum sex hormones(FSH,LH,estrogen(E2),progesterone(P4),testosterone(T))of African catfish(Clarias gariepinus).African catfish spawners were intermuscularly injected with different doses of HCG(500,1500,3000,6000 IU/kg female),and group is not injected as a control;males were injected at half the female dose.The results showed that,fish group injected by 6000 IU HCG/kg female had the highest gonadsomatic index,absolute fecundity and relative fecundity,while,the lowest value of absolute fecundity and relative fecundity were recorded with 500 IU HCG/kg female.The group injected with the highest amount of HCG(6000 IU/kg female)recorded the lowest value from egg diameter,while the highest egg diameter was observed in 500 IU HCG/kg female.In females,the group injected with 6000 IU HCG/kg female reflected the lowest level of FSH and the highest level of LH and the highest level of P4 compared to other treatments.Level of T recorded the highest level with 1500 IU HCG/kg female.The control group reflected the highest level of FSH and E2,while the control group reflected the lowest level of T and P4 level.In males,serum FSH,LH,P4 and E2 in male groups injected with HCG were relatively higher than those recorded in the control group.The highest level of T was recorded in treatment injected with the highest dose of HCG and decreased in other treatments until recorded the lowest level of T in the control group.It was observed,HCG hormone has successfully and accelerate induced spawning in African catfish(Clarias gariepinus)and increased in reproductive performance with the increase in HCG dosage and as compared to group not injected.展开更多
Background: The pathologies causing male infertility are various (congenital or acquired) and concern several hormone-producing organs: the hypothalamus, pituitary gland, testes and adrenals. The hormonal dosage inclu...Background: The pathologies causing male infertility are various (congenital or acquired) and concern several hormone-producing organs: the hypothalamus, pituitary gland, testes and adrenals. The hormonal dosage includes systematically testosterone, FSH and LH. These analyses often highlight hypogonadism hypogonadotropic or hypergonadotropic. They can sometimes be normal. The present study aimed to establish the hormonal profile of infertile men residing in Brazzaville. Patients and Methods: It was a cross-sectional descriptive study during five years (from 2018 to 2023). It concerned 344 infertilemen with an abnormal spermogram and spermocytogram. The method was performed from the blood dosage of testosterone, FSH, and LH using the Elisa technique. Results: The hormonal assessment was not normal in 48.83% (168/344) of cases. Increased values of testosterone were noted in 6.10% of cases and decreased in 12.20%. Abnormal high values of FSH were observed in 14.24% of cases and decreased values in 8.13%. LH was elevated, i.e. 2.03% of cases and decreased in 6.10% of subjects. Conclusions: A hormonal disturbance was observed in almost half of the infertile men. It concerned the hypothalamus-pituitary and the testes axis and indicated mainly a state of hypogonadism with high FSH and low LH.展开更多
确定外源性性激素 (不同剂量雌激素、孕激素和孕激素配伍雌激素 )影响垂体黄体生成素 ( L H)分泌变化的细胞形态学特征。方法 :应用特异性 r L Hβ抗体和 ABC亲和组织化学方法显示垂体 L H细胞 ,结合计算机图像分析系统观察和测定不同...确定外源性性激素 (不同剂量雌激素、孕激素和孕激素配伍雌激素 )影响垂体黄体生成素 ( L H)分泌变化的细胞形态学特征。方法 :应用特异性 r L Hβ抗体和 ABC亲和组织化学方法显示垂体 L H细胞 ,结合计算机图像分析系统观察和测定不同剂量雌激素、孕激素和孕激素配伍雌激素对垂体 L H细胞大小、细胞内液泡大小和细胞形状等形态参数的影响。结果 :不同类型性激素使去卵巢后增大的 L H细胞恢复至正常细胞的大小范围 ,但在不同性激素影响下 ,L H细胞内最终的激素水平和相应的细胞形态特征是不同的。低剂量雌激素使 L H细胞内大量贮存的激素处于易释放状态 ,细胞内出现大小不等的液泡。大剂量雌激素抑制 L H的大量贮存 ,细胞内充满分泌颗粒 ,很少液泡结构。孕激素使细胞内激素大量贮存但处于不易释放状态 ,细胞内含有大量分泌颗粒和少量液泡结构。孕激素配伍雌激素抑制细胞内 L H的大量贮存和释放 ,但细胞形状较大剂量雌激素作用组规则 ,细胞内除分泌颗粒外也存在少量液泡。结论 :雌 /孕激素均能逆转去卵巢后 L H的过量分泌 ,并使 L H细胞呈现正常生理变化范围内的不同细胞形态变化。展开更多
基金supported by the National Natural Science Foundation of China (No.21177119)the Ministry of Environmental Protection Funded Project (No.200909101)the National Natural Science Foundation of China(21007005)
文摘Objective To study the effects of environmental multi-media lead pollution on blood lead and sex hormone levels among lead exposed males engaged in E-waste dismantling, and the correlation between confounding factors and sex hormone levels. Methods An E-waste dismantling area in Taizhou of Zhejiang Province was selected as the research site. One hundred and fifty two samples were collected from the groundwater, soil, rice, corn, chicken, and pork in the dismantling area. The effects of the multi-media lead pollution on the male blood lead and sex hormone levels of FSH, LH, and T, as well as the correlation with confounding factors, were studied. Results The blood lead concentrations in the males aged under 31, from 31 to 45 and from 46 to 60 were 98.55, 100.23, and 101.45 I~/L, respectively. Of all the environmental media lead exposures, the groundwater, rice and soil were main contributing factors to the lead accumulation in humans. FSH and LH levels increased with the age while the T levels decreased with the age instead. There was a significant correlation between the FSH and LH levels and wearing masks. Conclusion There was correlation between the FSH, LH, and T levels, and the mean values of lead concentrations in environmental media, and the sex hormone levels were correlated with the confounding factor of wearing masks.
基金the National Key L aboratory of Contraceptive Drugs and Device( No.B2 -97-8) and the National Natural Science Foundation of China( No.3 9670 3 1 3 )
文摘Objective To identify the morphological characteristics of pituitary luteinizing hormone (LH) cells after exogenous gonadal hormone treatment Methods Effects of various doses of estrogen, progesterone and their combination on morphological parameters, including the size and shape of pituitary LH cells, the size of endocellular vacuoles, were observed and measured by immuno histochemistry and computer image analysis. Results Different kinds of gonadal hormones could recover the magnified LH cells to the normal level in ovariectomized rats. However, their final effects on the gonadotrophin levels and the cellular morphological characters of the LH cells were different. The low dose of estrogen elicited abundant hormone stored in the LH cells to an easy released status with a lot of different size of vacuoles. On the contrary, the high dose of estrogen inhibited the storage of LH, and the LH cells were filled with secretory granules and few vacuoles. The progesterone could promote the storage of LH in an uneasy released status. The administration of estrogen progesterone combination not only inhibited the storage of LH, but also the release of LH. In this group, the LH cells containing a large amount of secretory granules and a few vacuoles showed a better uniform shape compared with those administrated with high dose of estrogen. Conclusion: Different kinds of gonadal hormones could reverse the excessive secretion of LH and recover the morphological change of LH cells to the normally physiological condition.
基金supported by the National Natural Science Foundation of China(No.81072247)the Education Department of Henan Province,China(No.13A330653)
文摘The effects of fluoride exposure on thefunctions of reproductive and endocrine systemshave attracted widespread attention in academiccircle nowadays. However, it is unclear whether thegene-environment interaction may modify thesecretion and activity of hypothalamus-pituitary-ovarian （HPO） axis hormones. Thus, the aim of thisstudy was to explore the influence of fluorideexposure and follicle stimulating hormone receptor（FSHR） gene polymorphism on reproductivehormones in Chinese women. A cross sectionalstudy was conducted in seven villages of HenanProvince, China during 2010-2011. A total of 679women aged 18-48 years were recruited throughcluster sampling and divided into three groups, i.e.endemic fluorosis group （EFG）, defluoridationproject group （DFPG）, and control group （CG） basedon the local fluoride concentration in drinkingwater. The serum levels of gonadotropin releasinghormone （GnRH）, follicle stimulating hormone（FSH）, luteinizing hormone （LH）, and estradiol （E2）were determined respectively and the FSHRpolymorphism was detected by real time PCR assay.The results provided the preliminary evidenceindicating the gene-environment interaction onHPO axishormones in women.
文摘This study was conducted to investigate the effect of different doses injection of human chorionic gonadotropin(HCG)hormone on fecundity and serum sex hormones(FSH,LH,estrogen(E2),progesterone(P4),testosterone(T))of African catfish(Clarias gariepinus).African catfish spawners were intermuscularly injected with different doses of HCG(500,1500,3000,6000 IU/kg female),and group is not injected as a control;males were injected at half the female dose.The results showed that,fish group injected by 6000 IU HCG/kg female had the highest gonadsomatic index,absolute fecundity and relative fecundity,while,the lowest value of absolute fecundity and relative fecundity were recorded with 500 IU HCG/kg female.The group injected with the highest amount of HCG(6000 IU/kg female)recorded the lowest value from egg diameter,while the highest egg diameter was observed in 500 IU HCG/kg female.In females,the group injected with 6000 IU HCG/kg female reflected the lowest level of FSH and the highest level of LH and the highest level of P4 compared to other treatments.Level of T recorded the highest level with 1500 IU HCG/kg female.The control group reflected the highest level of FSH and E2,while the control group reflected the lowest level of T and P4 level.In males,serum FSH,LH,P4 and E2 in male groups injected with HCG were relatively higher than those recorded in the control group.The highest level of T was recorded in treatment injected with the highest dose of HCG and decreased in other treatments until recorded the lowest level of T in the control group.It was observed,HCG hormone has successfully and accelerate induced spawning in African catfish(Clarias gariepinus)and increased in reproductive performance with the increase in HCG dosage and as compared to group not injected.
文摘Background: The pathologies causing male infertility are various (congenital or acquired) and concern several hormone-producing organs: the hypothalamus, pituitary gland, testes and adrenals. The hormonal dosage includes systematically testosterone, FSH and LH. These analyses often highlight hypogonadism hypogonadotropic or hypergonadotropic. They can sometimes be normal. The present study aimed to establish the hormonal profile of infertile men residing in Brazzaville. Patients and Methods: It was a cross-sectional descriptive study during five years (from 2018 to 2023). It concerned 344 infertilemen with an abnormal spermogram and spermocytogram. The method was performed from the blood dosage of testosterone, FSH, and LH using the Elisa technique. Results: The hormonal assessment was not normal in 48.83% (168/344) of cases. Increased values of testosterone were noted in 6.10% of cases and decreased in 12.20%. Abnormal high values of FSH were observed in 14.24% of cases and decreased values in 8.13%. LH was elevated, i.e. 2.03% of cases and decreased in 6.10% of subjects. Conclusions: A hormonal disturbance was observed in almost half of the infertile men. It concerned the hypothalamus-pituitary and the testes axis and indicated mainly a state of hypogonadism with high FSH and low LH.
文摘确定外源性性激素 (不同剂量雌激素、孕激素和孕激素配伍雌激素 )影响垂体黄体生成素 ( L H)分泌变化的细胞形态学特征。方法 :应用特异性 r L Hβ抗体和 ABC亲和组织化学方法显示垂体 L H细胞 ,结合计算机图像分析系统观察和测定不同剂量雌激素、孕激素和孕激素配伍雌激素对垂体 L H细胞大小、细胞内液泡大小和细胞形状等形态参数的影响。结果 :不同类型性激素使去卵巢后增大的 L H细胞恢复至正常细胞的大小范围 ,但在不同性激素影响下 ,L H细胞内最终的激素水平和相应的细胞形态特征是不同的。低剂量雌激素使 L H细胞内大量贮存的激素处于易释放状态 ,细胞内出现大小不等的液泡。大剂量雌激素抑制 L H的大量贮存 ,细胞内充满分泌颗粒 ,很少液泡结构。孕激素使细胞内激素大量贮存但处于不易释放状态 ,细胞内含有大量分泌颗粒和少量液泡结构。孕激素配伍雌激素抑制细胞内 L H的大量贮存和释放 ,但细胞形状较大剂量雌激素作用组规则 ,细胞内除分泌颗粒外也存在少量液泡。结论 :雌 /孕激素均能逆转去卵巢后 L H的过量分泌 ,并使 L H细胞呈现正常生理变化范围内的不同细胞形态变化。
