Molecular Mechanism of Rice Necrotic Lesion for Optimized Yield and Disease Resistance

How to balance rice resistance and yield is an important issue in rice breeding.Plants with mutated necrotic lesion genes often have persistent broad-spectrum resistance,but this broad-spectrum resistance usually comes at the expense of yield.Currently,many necrotic lesion mutants in rice have been identified,and these genes are involved in disease resistance pathways.This review provides a detailed introduction to the characteristics,classification,and molecular mechanisms of necrotic lesion formation.Additionally,we review the molecular regulatory pathways of genes involved in rice disease resistance.Concurrently,we summarize the relationship between resistance and yield in rice using newly developed gene editing methods.We discuss a rational and precise breeding strategy to better utilize molecular design technology for breeding disease-resistant and high-yield rice varieties.

Yield Characteristics of a New High-quality Disease-resistant Wheat Variety Chuanmai 618

[Objectives]The paper was to analyze the yield characteristics of a new high-quality disease-resistant wheat variety Chuanmai 618.[Methods]The yield characteristics of Chuanmai 618 were analyzed using the AMMI model and GGE biplot based on data from a 2-year regional test and a 1-year production test.[Results]The analysis of the AMMI model for the 2-year regional test indicated that Chuanmai 618 had a moderate yield and good stability.During the production test,Chuanmai 618 had an average yield of 450.52 kg/666.7 m^(2),an effective spike of 235700 spike/666.7 m^(2),a 1000-seed weight of 47.93 g,and a kernel number per spike of 47.28.The AMMI analysis sequencing graph showed that the varieties were ranked in the following order:Zhongkemai 1816>Chuanmai 618>Shumai 1958>Chuanyu 42>Mianmai 367>Xikemai 5518.According to the GE analysis,Chuanmai 618 had comparative advantages.[Conclusions]The new wheat variety Chuanmai 618 is a high-quality disease-resistant variety with good yield and stability.

High Yield Cultivation and Pest Control Technology of Cashew

In order to enhance the yield and quality of cashew,it is essential to implement high-yield cultivation techniques effectively throughout the production process.Additionally,pest control measures should be employed to provide technical support for the industrialized development of cashew.

Resequencing-based QTL mapping for yield and resistance traits reveals great potential of Oryza longistaminata in rice breeding

As a natural genetic reservoir, wild rice contains many favorable alleles and mutations conferring high yield and resistance to biotic and abiotic stresses. However, there are few reports describing favorable genes or QTL from the AA genome wild rice O. longistaminata, which is characterized by tall and robust habit and long tassels and anthers and shows high potential for use in cultivated rice improvement. We constructed a stable BC_(2)F_(20) backcross inbred line(BIL) population of 152 lines from the cross of 9311 × O.longistaminat. Some BILs showed large panicles, large seeds, and strong resistance to rice false smut, bacterial leaf blight, rice blast spot, and brown planthopper. Genomic resequencing showed that the 152 BILs covered about 99.6% of the O. longistaminata genome. QTL mapping with 2432 bin markers revealed 13QTL associated with seven yield traits and eight with resistance to brown planthopper and to four diseases. Of these QTL, 12 for grain yield and 11 for pest and disease resistance are novel in Oryza species.A large-panicle NIL1880 line containing QTL qPB8.1 showed a nearly 50% increase in spikelet number and27.5% in grain yield compared to the recurrent parent 9311. These findings support the potential value of O. longistaminata for cultivated rice improvement.

Breeding of National Authorized New Wheat Variety Shannong 116 and Supporting Cultivation Technical Measures for High Yield, High Quality and High Efficiency

Shannong 116 is a strong gluten,high yield and multi-resistance wheat variety bred by Shandong Agricultural University,which was approved by the State in 2021 and by Shandong Province in 2022.Shannong 116 combines the excellent characteristics of the female parent(strong gluten,disease resistance and early maturity)and the male parent(high yield,water saving and lodging resistance),with a plant height of 76.9 cm,compact plant type,orderly spike layer and good maturity performance,which is suitable for large-scale promotion and market order planting in Huanghuai wheat area.In this paper,the characteristics of Shannong 116 are analyzed,and cultivation technical measures for high yield,high quality and high efficiency are put forward,in order to provide a technical support for the popularization and application of the variety.

High-yield Cultivation Techniques of Drip Irrigation under Ground Membrane for Cotton

Yuepuhu County in Xinjiang has a long history of cotton planting and is a national high-quality cotton base county.In 2002,it was appraised by the Ministry of Agriculture as the"Hometown of High-yield Cotton Production in China",with an annual cotton planting area of about 43000 ha.Traditional cotton planting has disadvantages such as waste of water resources,low water use efficiency,easy breeding of diseases and insect pests,and unfavorable ground temperature recovery at the seedling stage.In order to solve the low water use efficiency in cotton planting in Yuepuhu County,reduce the occurrence of diseases and insect pests,promote sustainable cotton production,and improve the economic benefits and local ecological benefits of cotton planting,Yuepuhu County promoted the application of the resource-saving technology drip irrigation under ground membrane cotton comprehensive cultivation technology mainly promoted by the Ministry of Agriculture.This paper mainly discusses the disadvantages of traditional cotton planting,the main technical content of drip irrigation under ground membrane technology,the problems found in the practice process and the solutions,so as make cotton growers in Yuepuhu County better understand the planting technology under plastic film,and to better promote the development of cotton industry in Yuepuhu County and the surrounding regions.

Research progress on reduced lodging of high-yield and-density maize

Increasing plant density is an effective way to enhance maize yield, but often increases lodging rate and severity, significantly elevating the risk and cost of maize production. Therefore, lodging is a major factor restricting future increases in maize yield through high-density planting. This paper reviewed previous research on the relationships between maize lodging rate and plant morphology, mechanical strength of stalks, anatomical and biochemical characteristics of stalks, root characteristics, damage from pests and diseases, environmental factors, and genomic characteristics. The effects of planting density on these factors and explored possible ways to improve lodging resistance were also analyzed in this paper. The results provide a basis for future research on increasing maize lodging resistance under high-density planting conditions and can be used to develop maize cultivation practices and lodging-resistant maize cultivars.

Photosynthetic Characteristics of a Super High Yield Cultivar of Winter Wheat During Late Growth Period

The mechanism of high yield of winter wheat in the field at late growth period was investigated by measuring the photosynthetic characteristics of photosystem Ⅱ （PSⅡ） and xanthophylls cycle, which could provide physiological reference for breeding. Weimai 8 （W8）, a super high yield cultivar, and Lumai 14 （L14）, a control cultivar were object. The photosynthetic rate （Pn）, parameters of chlorophyll fluorescence and chlorophyll content were measured. The Pn, maximum photochemical efficiency of PSII （Fv/Fm）, quantum yield of PSII electron transport （ΦPSⅡ）, efficiency of excitation energy capture by open PSII reaction centers （Fv＇/Fm＇）, and photochemical quenching coefficient （qP） were higher in Weimai 8 compared to that in Lumai 14, a commercial high yield cultivar. Furthermore, Weirnai 8 showed a lower non- photochemical quenching coefficient and a lower de-epoxidized ratio of the xanthophyll cycle pigments than of Lumai 14 at late growth period. At mature stage, chlorophyll content of different leaves decreased both in Weimai 8 and Lumai 14. Chlorophyll content in flag, second and third leaf from the top of plant decreased more in Lumai 14 than in Weimai 8. These results suggested that Weimai 8 had more antenna pigments to absorb light energy, and had higher photosynthetic capability and photochemical efficiency of PSⅡ. The yield of Weimai 8 was also higher than that of Lumai 14.

A Preliminary Study on Dynamics and Models of N,P,K Absorption for High-Yield Cotton

The field experiments were carried out to investigate the dynamics and models of N, P and K absorption for the cotton plants with a lint of 3 000 kg ha-1 in Xinjiang. The main results were as follows: The contents of N, P2O5, K2O in cotton leaves, stems, squares and bolls decreased obviously with the time over the whole growth duration and the falling extent was greater in high-yield cotton than in CK. Contents of N in leaves, squares and bolls, in particular in the leaves of fruit-bearing shoot was higher in high-yield cotton than in CK. Contents of P2O5 in squares and bolls and that of K2O in stems were higher in high-yield cotton than in CK during the whole growing period. The accumulations of N, P2O5 and K2O in the cotton plants could be described with a logistic curve equation. There was the fastest nutrient uptake at about 90 d for N, 92 d for P2O5 and 85 d for K2O after emergence, respectively. Total nutrient accumulation of N, P2O5 and K2O was 385.8, 244. 7 and 340.3 kg ha-1, respectively. Approximately 12. 5 kg N, 8. 0 kg P2O5 and 11.1 kg K2O were needed for producing 100 kg lint with the leaves and stems under the super high yield condition of 3 000 kg ha-1 in Xinjiang.

Using irrigation intervals to optimize water-use efficiency and maize yield in Xinjiang,northwest China

Worldwide, scarce water resources and substantial food demands require efficient water use and high yield.This study investigated whether irrigation frequency can be used to adjust soil moisture to increase grain yield and water use efficiency(WUE) of high-yield maize under conditions of mulching and drip irrigation.A field experiment was conducted using three irrigation intervals in 2016: 6, 9, and 12 days(labeled D6, D9, and D12) and five irrigation intervals in 2017: 3, 6, 9, 12, and 15 days(D3, D6, D9, D12, and D15).In Xinjiang, an optimal irrigation quota is 540 mm for high-yield maize.The D3, D6, D9, D12, and D15 irrigation intervals gave grain yields of 19.7, 19.1–21.0, 18.8–20.0, 18.2–19.2, and 17.2 Mg ha^-1 and a WUE of 2.48, 2.53–2.80, 2.47–2.63, 2.34–2.45, and 2.08 kg m-3, respectively.Treatment D6 led to the highest soil water storage, but evapotranspiration and soil-water evaporation were lower than other treatments.These results show that irrigation interval D6 can help maintain a favorable soil-moisture environment in the upper-60-cm soil layer, reduce soilwater evaporation and evapotranspiration, and produce the highest yield and WUE.In this arid region and in other regions with similar soil and climate conditions, a similar irrigation interval would thus be beneficial for adjusting soil moisture to increase maize yield and WUE under conditions of mulching and drip irrigation.

Quantitative design of yield components to simulate yield formation for maize in China

Maize(Zea mays L.) stands prominently as one of the major cereal crops in China as well as in the rest of the world.Therefore,predicting the growth and yield of maize for large areas through yield components under high-yielding environments will help in understanding the process of yield formation and yield potential under different environmental conditions.This accurate early assessment of yield requires accuracy in the formation process of yield components as well.In order to formulate the quantitative design for high yields of maize in China,yield performance parameters of quantitative design for high grain yields were evaluated in this study,by utilizing the yield performance equation with normalization of planting density.Planting density was evaluated by parameters including the maximum leaf area index and the maximum leaf area per plant.Results showed that the variation of the maximum leaf area per plant with varying plant density conformed to the Reciprocal Model,which proved to have excellent prediction with root mean square error(RMSE) value of 5.95%.Yield model estimation depicted that the best optimal maximum leaf area per plant was 0.63 times the potential maximum leaf area per plant of hybrids.Yield performance parameters for different yield levels were quantitatively designed based on the yield performance equation.Through validation of the yield performance model by simulating high yields of spring maize in the Inner Mongolia Autonomous Region and Jilin Province,China,and summer maize in Shandong Province,the yield performance equation showed excellent prediction with the satisfactory mean RMSE value(7.72%) of all the parameters.The present study provides theoretical support for the formulation of quantitative design for sustainable high yield of maize in China,through consideration of planting density normalization in the yield prediction process,providing there is no water and nutrient limitation.

An Organic Solvent-Assisted Intercalation and Collection (OAIC) for Ti_(3)C_(2)T_(x) MXene with Controllable Sizes and Improved Yield

A good method of synthesizing Ti_(3)C_(2)T_(x)(MXene)is critical for ensuring its success in practical applications,e.g.,electromagnetic interference shielding,electrochemical energy storage,catalysis,sensors,and biomedicine.The main concerns focus on the moderation of the approach,yield,and product quality.Herein,a modified approach,organic solvent-assisted intercalation and collection,was developed to prepare Ti_(3)C_(2)T_(x) flakes.The new approach simultaneously solves all the concerns,featuring a low requirement for facility(centrifugation speed<4000 rpm in whole process),gram-level preparation with remarkable yield(46.3%),a good electrical conductivity(8672 S cm^(−1)),an outstanding capacitive performance(352 F g^(−1)),and easy control over the dimension of Ti_(3)C_(2)T_(x) flakes(0.47–4.60μm^(2)).This approach not only gives a superb example for the synthesis of other MXene materials in laboratory,but sheds new light for the future mass production of Ti_(3)C_(2)T_(x) MXene.

Study on Dynamic Prediction of Two-Phase Pipe Flow in Inclined Wellbore with Middle and High Yield

Gas-liquid two-phase flow is ubiquitous in the process of oil and gas exploitation,gathering and transportation.Flow pattern,liquid holdup and pressure drop are important parameters in the process of gas-liquid two-phase flow,which are closely related to the smooth passage of the two-phase fluid in the pipe section.Although Mukherjee,Barnea and others have studied the conventional viscous gas-liquid two-phase flow for a long time at home and abroad,the overall experimental scope is not comprehensive enough and the early experimental conditions are limited.Therefore,there is still a lack of systematic experimental research and wellbore pressure for gas-liquid two-phase flow under the conditions of middle and high yield and high gas-liquid ratio in conventional viscosity,and the prediction accuracy is low.In view of this,this study carried out targeted systematic research,and from the flow pattern,liquid holdup and pressure drop aspects,established the relevant model,obtained a set of inclined wellbore gas-liquid two-phase pipe flow dynamic prediction method.At the same time,firstly,the model is tested by experimental data,and then the model is compared and verified by a number of field measured wells,which proves that the model is reliable and the prediction accuracy of wellbore pressure is high.

Key Technology for Liquid Spawn Production and Green and High-yield Cultivation of Pleurotus eryngii

[Objectives]Pleurotus eryngii is currently the second largest edible fungus variety cultivated in China,and it mainly adopts the bag cultivation mode.This study aims to effectively solve the problems of high cost of solid spawn,long spawn production cycle,low application level of green and high-yield cultivation technology and equipment,and unstable output and quality in the factory production process and to further promote industrial upgrading.[Methods]Since June,2018,a series of research and technical optimizations had been carried out by a technical research team consisting of personnels from Vegetable Office of Guannan County,Institute of Edible Fungi of Shanghai Academy of Agricultural Sciences and Jiangsu Xiangru Biotechnology Co.,Ltd.,around the production of liquid spawn,introduction of excellent varieties of P.eryngii,medium pretreatment,autoclaving,clean room inoculation,digital cultivation,harvesting,packaging,etc.[Results]The key technology of industrial production,liquid spawn production and green and high-yield cultivation of P.eryngii are summarized.[Conclusions]The economic benefit of liquid spawn of P.eryngii is extremely considerable compared with liquid spawns such as branch spawn.

Demonstration and Radiation Extension mode of Agricultural Technology——A Case of the Science and Technology Project of High Yield in Hebei Province

Firstly,relying on the science and technology project of high yield in Hebei Province,connotation of agricultural technology demonstration and extension mode and the high yield grain project in Hebei Province is introduced.Extension mode of agricultural technology demonstration and radiation is constructed.Agricultural technology demonstration and radiation mode includes the radiation center,primary irradiation and secondary irradiation.Secondly,management system and operational mechanism of agricultural technology demonstration and radiation mode are discussed,mainly expressed in establishing leading group in each demonstration county(city),carrying out leader contract responsibility system,establishing expert advisor steering group and setting up core experts group for subject,establishing technical experts group for subject,setting up leading group in the radiation area,carrying out chief expert responsibility system and technician matrix responsibility system,establishing the operating mechanism of "open,flow,competition and collaboration" with "test area-expert two-way selection" as the core content.Finally,countermeasures to improve the agricultural technology demonstration and radiation mode is put forward,such as establishing the rural technology demonstration base,strengthening cooperation with enterprises,and adopting flexible technical training,so as to promote the spread of agricultural high-tech,to increase the contribution rate of agricultural technology,and to offer ideas for agricultural technology extension model at the new era.

Evaluation of yield,stability and adaptability of national winter rapeseed regional trials in the upper Yangtze River region in 2017-2018

Rapeseed variety needs to be tested by regional trial in multiple sites for many years before being applied in market in China.Performants of rapeseed were affected by the interaction of sites and varieties.Evaluation of regional trials is very important for guiding rapeseed breeding.GGE(genotype main effects and genotypeenvironment interaction)biplot was used to evaluate yield,stability,adaptability,representativeness and discrimination of national winter rapeseed trials in the upper reaches of Yangtze River in 2017-2018.Results showed that the main effects of genotype(G),environment(E)and genotypeenvironment interaction(GE)were significant(P<0.01)for yield.Among them,E accounted for 46.95%total variation on average,G and GE accounted for 19.34%and 33.71%respectively.Eight varieties were found with high-yield,excellent stability and adaptability,including‘Yiyou 29’,‘Xiwang 920’,‘Liyouza 108’,‘Nanyou 546’,‘Dadi 195’,‘Jiayou 1’,‘Huayouza 28’and‘Yuhua 2’.Test sites included Nanchong,Mianyang,Wanzhou,Shuangliu and Chengdu in Sichuan Province and Zunyi together with Guiyang in Guizhou Province were selected for their excellent representativeness and discrimination.These results would provide theoretical basis for rapeseed breeding.

Characteristics of lodging resistance of high-yield winter wheat as affected by nitrogen rate and irrigation managements

High yields of wheat are mainly obtained through a high level of nitrogen and irrigation supplementation.However,excessive nitrogen and irrigation supplication increase the risk of lodging.The main objectives of this work were to clarify the capacity of lodging resistance of wheat in response to nitrogen and irrigation,as well as to explore the effective ways of improving lodging resistance in a high-yield wheat cultivar.In this study,field experiments were conducted in the 2015-2016 and 2016-2017 growing seasons.A wheat cultivar Jimai 22(JM22),which is widely planted in the northern of Huang-Huai winter wheat region,was grown at Tai’an,Shandong Province,under three nitrogen rates and four irrigation treatments.The lodging risk was increased with increased nitrogen rate,as indicated by increasing lodging index(LI)and lodging rate across both growing seasons.With nitrogen increasing,the plant height,the basal internode length and the center of gravity height,which were positively correlated with LI,increased significantly.While the density of the basal 2nd internode(for culm and leaf sheath)and cell wall component contents,which were negatively correlated with LI,decreased conspicuous along with nitrogen increased.Increasing irrigation supplementation increased the 2nd internode culm wall thickness,breaking strength and leaf sheath density within limits which increased stem strength.Among the treatments,nitrogen application at a rate of 240 kg ha^(-1) and irrigation application at 600 m^(3)ha^(-1) at both the jointing and anthesis stages resulted in the highest yield and strongest stem.A suitable plant height ensures suffcient biomass for high yield,and higher stem stiffness,which was primarily attributed to thicker culm wall,greater density of the culm and leaf sheaths and higher cell wall component contents are the characteristics that should be taken into account to improving wheat lodging resistance.

Introgression of High Yield Genes from Lycopersicon hirsutum acc.LA1777 Using CAPS Marker

The idea behind this study is to show that using high yield genes from a wild tomato can enrich tomato breeding resources and accelerate tomato breeding programs. In this study, the near-isogenic line TA1229 containing a 24-cM introgression at the bottom of chromosome 1 from Lycopersicon acc. LA1777, affects several higher yield traits. The TA1229×9706 BC1 population was analyzed by marker-assisted selection and the traits of the population were evaluated. Twenty-three recombinant individuals that carried a shorter segment than TA1229 were obtained. Among them, 16 lines with the chromosome 1 recombinant segment can increase tomato yield and a QTL affecting yield was found between TG53 and TG158. Sixteen recombinant lines are useful to improve the tomato variety.

High Yield Transfer of Clean Large-Area Epitaxial Oxide Thin Films

In this work,we have developed a new method for manipulating and transferring up to 5 mm×10 mm epitaxial oxide thin films.The method involves fixing a PET frame onto a PMMA attachment film,enabling transfer of epitaxial films lifted-off by wet chemical etching of a Sr3Al2O6 sacrificial layer.The crystallinity,surface morphology,continuity,and purity of the films are all preserved in the transfer process.We demonstrate the applicability of our method for three different film compositions and structures of thickness~100 nm.Furthermore,we show that by using epitaxial nanocomposite films,lift-off yield is improved by~50% compared to plain epitaxial films and we ascribe this effect to the higher fracture toughness of the composites.This work shows important steps towards large-scale perovskite thin-film-based electronic device applications.

Development of high yield and tomato yellow leaf curl virus(TYLCV)resistance using conventional and molecular approaches:A review

Tomato(Solanum lycopersicum L.)belonging to the family Solanaceae is the second most consumed and cultivated vegetable globally.Since the ancient time of its domestication,thousands of cultivated tomato varieties have been developed targeting an array of aspects.Among which breeding for yield and yield-related traits are mostly focused.Cultivated tomato is extremely genetically poor and hence it is a victim for several biotic and abiotic stresses.Among the biotic stresses,the impact of viral diseases is critical all over tomato cultivating areas.Improvement of tomato still largely rely on conventional methods worldwide while molecular approaches,particularly Marker Assisted Selection(MAS)has become popular across the globe as a fast,low cost and precise tool which is essential in present day plant breeding.In this review paper,breeding tomato for high yield and viral disease resistance,particularly to tomato yellow leaf curl virus disease(TYLCVD)using conventional and molecular approaches will be discussed.Lining up of this set of information will be useful to those who are interested in tomato variety development with high yielding and TYLCVD resistance.