Objective To evaluate the long-term therapeutic effect and histologic result of ADM combined with autologous thin split-thickness skin graft.Methods 23 patients were treated with acellalar dermal matrix(ADM) combined ...Objective To evaluate the long-term therapeutic effect and histologic result of ADM combined with autologous thin split-thickness skin graft.Methods 23 patients were treated with acellalar dermal matrix(ADM) combined with autoiogous展开更多
Eucalyptus benthamii is a forest species of economic interest that has difficulty with seed production and also is considered to have difficulty with adventitious rooting using propagation techniques, such as cutting ...Eucalyptus benthamii is a forest species of economic interest that has difficulty with seed production and also is considered to have difficulty with adventitious rooting using propagation techniques, such as cutting or mini-cutting. We aimed to assess the adventitious rooting percentage under different storage times in low temperatures and at various IBA （indole-3-butyric acid） concentrations to determine the optimal time of permanence for rooting Eucalyptus benthamii minicuttings in a greenhouse. Shoots collected from mini-stumps cultivated in a semi-hydroponic system were used to obtain the mini-cuttings. For the first experiment, the mini-cuttings were stored at 4℃ for 0 （immediate planting）, 24, 48, 72, 96 and 120 h. The second experiment evaluated the rooting dynamic to determine the optimal time of permanence for minicuttings in a greenhouse. The basal region of the mini-cutting was treated with various IBA solutions： 0 （free of IBA）, 1,000, 2,000, 3,000 and 4,000 mg.L^-1. Every seven days （0 （immediate planting）, 7, 14, 21 and 28 days）, destructive sampling of the mini-cuttings was performed to evaluate the histology of the adventitious rooting. Eucalyptus benthamii minicuttings should be rooted immediately after the collection of the shoots. The 2,000 mg.L^-1 IBA concentration induced a greater speed and percentage of adventitious rooting, and an interval of 35 to 42 days was indicated for permanence of the mini-cuttings in the greenhouse. Expo- sure to low temperature induced adventitious root formation with diffuse vascular connections.展开更多
Many egg-laying reptiles possess temperature-dependent sex determination(TSD)in which outcome of gonadogenesis is determined by incubation temperature during a temperature-sensitive period of development.Prior studies...Many egg-laying reptiles possess temperature-dependent sex determination(TSD)in which outcome of gonadogenesis is determined by incubation temperature during a temperature-sensitive period of development.Prior studies on Malayemys macrocephala showed that incubation temperatures influence gonadal development and suggested that M.macrocephala exhibits TSD.However,information on the temperature-sensitivity period in this species was unknown until the current study.Turtle eggs were collected from rice fields in central Thailand from December 2016 to February 2017.In the laboratory,eggs were incubated at male-biased temperature(26°C)and shifted to female-biased temperature(32°C),or vice versa.Single shift experiments were performed systematically during embryonic stages 13–21.After hatching,sex of individual turtles was determined by histological analysis.We found that the sex determination of M.macrocephala is affected by temperature up to stage 16 of embryonic development.展开更多
文摘Objective To evaluate the long-term therapeutic effect and histologic result of ADM combined with autologous thin split-thickness skin graft.Methods 23 patients were treated with acellalar dermal matrix(ADM) combined with autoiogous
基金financially by FAPESP (So Paulo Research Foundation,Brazil)CAPES (Coordination for the Improvement of Higher Level Personnel,Brazil)
文摘Eucalyptus benthamii is a forest species of economic interest that has difficulty with seed production and also is considered to have difficulty with adventitious rooting using propagation techniques, such as cutting or mini-cutting. We aimed to assess the adventitious rooting percentage under different storage times in low temperatures and at various IBA （indole-3-butyric acid） concentrations to determine the optimal time of permanence for rooting Eucalyptus benthamii minicuttings in a greenhouse. Shoots collected from mini-stumps cultivated in a semi-hydroponic system were used to obtain the mini-cuttings. For the first experiment, the mini-cuttings were stored at 4℃ for 0 （immediate planting）, 24, 48, 72, 96 and 120 h. The second experiment evaluated the rooting dynamic to determine the optimal time of permanence for minicuttings in a greenhouse. The basal region of the mini-cutting was treated with various IBA solutions： 0 （free of IBA）, 1,000, 2,000, 3,000 and 4,000 mg.L^-1. Every seven days （0 （immediate planting）, 7, 14, 21 and 28 days）, destructive sampling of the mini-cuttings was performed to evaluate the histology of the adventitious rooting. Eucalyptus benthamii minicuttings should be rooted immediately after the collection of the shoots. The 2,000 mg.L^-1 IBA concentration induced a greater speed and percentage of adventitious rooting, and an interval of 35 to 42 days was indicated for permanence of the mini-cuttings in the greenhouse. Expo- sure to low temperature induced adventitious root formation with diffuse vascular connections.
基金This research was supported by the 90th Anniversary of Chulalongkorn University Fund(Ratchadaphiseksomphot Endowment Fund)An educa-tional grant from the Human Resource Development in Science Project(Science Achievement Scholarship of Thailand)the Research Assistantship Fund from Fac-ulty of Science,Chulalongkorn University,to R.P.are fully acknowledged.
文摘Many egg-laying reptiles possess temperature-dependent sex determination(TSD)in which outcome of gonadogenesis is determined by incubation temperature during a temperature-sensitive period of development.Prior studies on Malayemys macrocephala showed that incubation temperatures influence gonadal development and suggested that M.macrocephala exhibits TSD.However,information on the temperature-sensitivity period in this species was unknown until the current study.Turtle eggs were collected from rice fields in central Thailand from December 2016 to February 2017.In the laboratory,eggs were incubated at male-biased temperature(26°C)and shifted to female-biased temperature(32°C),or vice versa.Single shift experiments were performed systematically during embryonic stages 13–21.After hatching,sex of individual turtles was determined by histological analysis.We found that the sex determination of M.macrocephala is affected by temperature up to stage 16 of embryonic development.
