The radioisotopic tracing method of Hirulog-like peptide（HLP） was established, wihch could be a reference for the pharmacokinetic analysis in small molecule polypeptide research.（）125I-HLP was prepared by Iodogen ...The radioisotopic tracing method of Hirulog-like peptide（HLP） was established, wihch could be a reference for the pharmacokinetic analysis in small molecule polypeptide research.（）125I-HLP was prepared by Iodogen method.The radiochemical purity and stability in vitro of（）125I-HLP were determined by HPLC and SDS-PAGE.The radiochemical purity of(（）125I-HLP) was shown above 95% and was stable for 53 hours.It is the first time using SPE to separate the labeled compound from radioiodide and small molecule polypeptides.The HLP recovery was（92.60±6.31）% by SPE and the coefficient of variation was lower than 10% in a concentration range from 10 to 100 000 ng/mL.The detectable limitation reached 52.79 ng/mL.展开更多
The chloroplasts genome （plastome） occurs at high copy numbers per cell. Several chloroplast genome copies are densely packed into nucleoprotein particles called nucleoids. How genome packaging occurs and which prot...The chloroplasts genome （plastome） occurs at high copy numbers per cell. Several chloroplast genome copies are densely packed into nucleoprotein particles called nucleoids. How genome packaging occurs and which proteins organize chloroplast nucleoids are largely unknown. Here, we have analyzed the Chlamydornonas reinhardtii homolog of the bacterial architectural DNA-binding protein HU, the histone-like protein HLP. We show that the Chlarnydornonas HLP protein is targeted to chloroplasts and associates with nucleoids. Knockdown of HLP gene expression by RNA interference （RNAi） alters the structure of chloroplast nucleoids and appears to reduce the level of compaction of chloroplast DNA. Unexpectedly, also chloroplast genome copy numbers are significantly decreased in the RNAi strains, suggesting that, in addition to its architectural role in nucleoid formation, the HIP protein is also involved in chloroplast genome maintenance.展开更多
目的评价CVVH和PE治疗HTG-AP的有效性。方法搜索Cochrane Library、PubMed、web of science、embase、中文数据库、知网、万方、维普、中文数据库,搜索日期至2022年7月,获取CVVH+PE治疗HTG-AP的国内外有关医学文献可予以数据库搜索等检...目的评价CVVH和PE治疗HTG-AP的有效性。方法搜索Cochrane Library、PubMed、web of science、embase、中文数据库、知网、万方、维普、中文数据库,搜索日期至2022年7月,获取CVVH+PE治疗HTG-AP的国内外有关医学文献可予以数据库搜索等检索方式;按照Cochrane Reviewer’s Handbook的规范,通过Stata16.0零软件和RevMan 5.0软件统计分析。结果收入了17篇论文,患者796例,CVVH+PE组409例,对照组392例。结果显示,CVVH+PE组总体治愈率明显提高:RR=1.33(1.22~1.45),且具有统计学意义Z=6.54,P=0.001<0.05,CVVH+PE的疗效显著优于单纯内科治疗,CVVH+PE是内科治疗的1.33倍。治疗后,TG效应量为-8.66(-9.66),且效应量显著(Z=-4.537,P<0.05),CVVH+PE组治疗后的TG比对照组显著低8.66。TNF-α效应量为-121.94,且效应量显著(Z=-2.662,P<0.05),CVVH+PE组治疗后的TNF-α比对照组显著低121.94。结论HTG-AP患者有效的治疗手段是CVVH+PE,目前需进一步开展大样本的RCT来评价CVVH+PE治疗HTG-AP的有效性和安全性。展开更多
文摘The radioisotopic tracing method of Hirulog-like peptide（HLP） was established, wihch could be a reference for the pharmacokinetic analysis in small molecule polypeptide research.（）125I-HLP was prepared by Iodogen method.The radiochemical purity and stability in vitro of（）125I-HLP were determined by HPLC and SDS-PAGE.The radiochemical purity of(（）125I-HLP) was shown above 95% and was stable for 53 hours.It is the first time using SPE to separate the labeled compound from radioiodide and small molecule polypeptides.The HLP recovery was（92.60±6.31）% by SPE and the coefficient of variation was lower than 10% in a concentration range from 10 to 100 000 ng/mL.The detectable limitation reached 52.79 ng/mL.
文摘The chloroplasts genome （plastome） occurs at high copy numbers per cell. Several chloroplast genome copies are densely packed into nucleoprotein particles called nucleoids. How genome packaging occurs and which proteins organize chloroplast nucleoids are largely unknown. Here, we have analyzed the Chlamydornonas reinhardtii homolog of the bacterial architectural DNA-binding protein HU, the histone-like protein HLP. We show that the Chlarnydornonas HLP protein is targeted to chloroplasts and associates with nucleoids. Knockdown of HLP gene expression by RNA interference （RNAi） alters the structure of chloroplast nucleoids and appears to reduce the level of compaction of chloroplast DNA. Unexpectedly, also chloroplast genome copy numbers are significantly decreased in the RNAi strains, suggesting that, in addition to its architectural role in nucleoid formation, the HIP protein is also involved in chloroplast genome maintenance.
