Spatial-Temporal Quantitative Global Energy Differences between the Living and Dead Human Brain

The dynamic differences between a living human brain and a clinically dead (fixed) human brain were measured over international Quantitative Electroencephalographic (QEEG) sites for 1 Hz increments between 1 Hz and 50 Hz. Although the expected greater power (μV2•Hz–1) for the living brain was apparent, the difference was particularly obvious for theta and low beta bands. The integrated square root values over the entire band indicated the difference in intrinsic charge-based energy between the living (higher) and dead brain was ~2•10–23 J. This quantity is remarkably proximal to the Cosmic Background Microwave value and would be consistent with the Hameroff-Penrose definition of consciousness that suggests a permeating presence derived from discrete physical events. A power value obtained by multiplying this increment of energy by the frequency of the universal hydrogen line resulted in ~10–12 W•m–2 when applied to the surface area of the human cerebrum. This value is the median flux density that has been measured from human brains during cognition and defines the ultra-weak photon emissions displayed by cells, tissue, and organisms. These results suggest that modern technology may now be sufficiently precise to discern the critical parameters that differentiate the living brain from the fixed “dead” brain. This information might be useful for future designs of virtual consciousness and simulations within cerebral space.

Biosorption of Cd from Aqueous Solution by Live and Dead Biomass of Fusarium oxysporum Isolated from the Sludge of Abandoned Mine

Owing to exploring the biosorption mechanism of Fusarium oxysporum to Cd,the adsorption capacities of live and dead biomass of F.oxysporum strain KF2 were detected.The result showed both the live and dead biomass of strain KF2 could tolerate the Cd concentration up to 200 mg/L,and had the largest adsorption capacities for Cd,at 2.21 and 1.86 mg/g,respectively,with the inoculation amount of 3 g,the pH at 6,and the initial Cd concentration of 100 mg/L.The pseudo-second-order kinetic model(live biomass r^(2)>0.99,dead biomass r^(2)>0.90)was more suitable for describing the adsorption process of strain KF2.It indicated that the physicochemical adsorption on the cell surface might be the main pattern for Cd removal.Furthermore,the FTIR results showed that the main functional groups for cell wall to bind Cd were carboxyl,hydroxyl,amino,and phosphate.

A New Historicism Interpretation of Is He Living or Is He Dead

Is He Living or Is He Dead is mainly about an outstanding painter Francois Millet who pretend to be dead in order to get reputation and money,because at that time a great artist has never been acknowledged until after he was starved and dead. Based on the critic approach of New Historicism, this thesis have analyzed how Millet subvert power and be contained by power, the capitalism and market, which criticized the phenomenon that the value of art is not decided by itself, but by power. No matter it is good or bad, if it is denied by power, it is valueless.

Being or Nothingness:Value Choice in Mark Twain's Is He Living or Is He Dead

In the interpretation of Mark Twain’s Is He Living or Is He Dead,the author points out that in the cage of human predic-ament the choice for being or nothingness lies in the fundamental precondition of ensuring subjectivity first and foremost,with equal emphasis on the organic whole of the signifier and the signified in what he or she is doing,so as to realize the subject’s true value of life.

Isotopic Variations of Oxygen (δ^(18)O) in Benthic Foraminifera under Antiestuarine Conditions in the Colorado River Delta

Benthic foraminifera are excellent environmental indicators;CaCO3 test records the isotopic concentration of their surroundings and can be used to analyze environmental changes that occur during a certain time period. Stable isotopes, particularly those of oxygen (δ18O), are useful for interpreting ancient environments, given that they are used as “proxy” environmental variables (temperature and salinity). In this study, we provide ranges of isotopic variation in benthic foraminifera communities from the Colorado River delta. Four sampling campaigns were conducted in one year (2009-2010) in the adjacent subtidal zone of Baja California. Four transects with a total of sixteen sample station were drawn perpendicular to the coastline. Here, we recorded the following in situ: sand-type, salinity, and temperature. In the laboratory, 300 individuals per sample were separated, and sub-samples were taken for isotopic analysis. Data was processed using the software’s R 2.12.2, PAST 1.81 and Arc Map 9.3. Forty species were identified in the dead assemblages (Thanatocoenosis), whereas thirteen species were found in the living assemblages (Biocoenosis). The most abundant species in both communities belong to the genera Ammonia and Cribroelphidium. In the living assemblages, isotopic variation −2.15% to 5.94% within a temperature interval of 11°C, indicated antiestuarine conditions. In the dead assemblages, isotopic composition −3.04 to −0.74 served as a sign of estuarine conditions prior to damming.

甲醛固定对Live/Dead BacLight Bacterial Viability Kit死活细菌染液荧光显微计数海洋细菌的影响

利用Live/Dead BacLight Bacterial Viability Kit死活细菌染液对采自湛江东海大堤海水、沉积物细菌和大型海藻拟刚毛藻(Cladophoropsis zollingeri)内生细菌数量进行了甲醛固定处理前后的荧光显微计数对比分析。结果表明,新鲜样品(不加甲醛固定)、甲醛刚固定样品、甲醛固定1周样品和甲醛固定2周样品中海洋细菌数量差异不显著(p>0.05)。甲醛固定对Live/Dead BacLight Bacterial Viability Kit死活细菌染液荧光显微计数海洋细菌数量无显著影响,固定后的样品可在2周内完成计数。

Applicability of LIVE/DEAD BacLight stain with glutaraldehyde fixation for the measurement of bacterial abundance and viability in rainwater

Rainwater contains substantial bacteria and rain is an efficient pathway for the dissemination of bacteria from the atmosphere to land and water surfaces.However,quantitative information on rainwater bacteria is very limited due to the lack of a reliable method.In this study,the epifluorescence microscopy enumeration with the LIVE/DEAD BacLight Bacterial Viability Kit stain was verified to quantify the abundance of viable and non-viable bacterial cells in rainwater,with the 4＇,6-diamidino-2-phenylindole（DAPI） stain for the reference of total cell counts.Results showed that the total counts of bacterial cells by LIVE/DEAD BacLight staining were consistent with those by DAPI staining,and the average detection efficiency was（109 ± 29）%.The ratio of cell count with glutaraldehyde fixation to that without fixation was（106 ± 5）%on average.The bacterial concentration in negative control was usually an order of magnitude lower than that in rainwater samples.However,in case of small precipitation,the abundance in negative control could be more than that in rainwater samples.These results indicate that the enumeration with LIVE/DEAD BacLight bacterial viability assay coupled with glutaraldehyde fixation and careful negative control investigation is an approach applicable to the measurement of the concentration and viability of bacterial cells in rainwater.

-lyperspectral Response： Key for Mapping Coral Rubber, .ive and Dead Corals

Measurement, data collection, and analyzing statistically the spectral response of different bottom types are all important steps in mapping shallow water bottom types with remote sensing. Five small islands in the Spermonde archipelago, South Sulawesi, in Indonesia, were selected to measure the reflectance spectral of benthic communities. The objective of this study was to determine optical properties of the live corals, dead corals covered with algae, coral rubber covered with algae, broken shell, sand, seagrass and macro algae. Pearson correlation coefficient and cluster analysis were used to determine spectral similarity in and among coral species based on spectral responses at observed wavelengths and to examine the similarities between the categories. Several benthic communities appear to be highly correlated with one another when the entire spectrum is considered, which may lead to classification errors. Porites meyeri, dead corals and coral rubber （〉 3 months ago） share a high degree of similarity in reflectance. The other coastal benthic communities are readily discriminated.

一种零火线反接检测系统及方法

介绍了一种零火线反接检测系统及方法,旨在解决电路中零火线反接导致的安全隐患问题。首先分析了零火线反接的原理和危害,然后提出了一种基于电路监测和报警机制的解决方案。该系统通过监测电路中的电压和电流,实时检测零火线的接线状态,并通过报警机制及时发出警报。实验结果表明,该系统能准确检测零火线反接情况,有效保障电路的安全运行。

Rapid and accurate evaluation of vaporized hydrogen peroxide on the efficiency of disinfection, using a sensitive dual-channel laser scanning cytometer

Vaporized hydrogen peroxide(VHP)is a highly active disinfectant,and VHP decontamination systems have been widely applied in hospitals,microbiological laboratories,and pharmaceutical industries.However,the decomposition of VHP into non-toxic by-products is essential.Evaluation of the disinfection efficacy of VHP is crucial to ensuring the reliability of VHP disinfection and controlling microbial contamination.In this study,a rapid and sensitive strategy is proposed to evaluate the efficacy of VHP in surface disinfection by detecting the survived and killed bacteria from VHP-exposed biological indicators(BIs).A dual-channel solid-phase cytometer is designed,and fluorescent dyes are used as indicators to automatically and accurately distinguish live cells from dead cells in themixtures of bacteria.To verify the availability and effectiveness of the laser scanning cytometry,experiments on its application in estimating the efficacy of VHP disinfection practice have been carried out in this study,and its estimation effect compared with that of the traditional plate counting method.Results show that the proposed assay might distinctly identify live or killed cells labeled by green and red fluorescent dyes and examined the disinfection efficacy in 30 min by calculating the bactericidal rate.Compared with the plate counting method,the proposed approach is accurate and practical,with an average detection efficiency of 98.47%±1.55%.Moreover,an excellent correlation between the concentrations of B.subtilis var niger(ATCC 9372)measured by the proposed detection system and by the plate counting method is noticed(R2=0.9971),indicating that this approach had advantages in the detection of trace microorganisms.To summarize,the proposed strategy appears practical and significant in many fields in which microbial counting and identification are required.

不同温度及厌氧/好氧运行条件对聚磷菌衰减特性的影响

以富含90%±2%纯度聚磷菌(PAOs)的强化生物除磷系统(EBPR)为研究对象,考察了10℃厌氧、10℃好氧、20℃厌氧、20℃好氧4种运行条件下PAOs的衰减特征。结果表明:温度越高对应衰减速率越快,4个系统在1～9d里衰减速率的平均值分别为:10℃厌氧:0.053/d;10℃好氧:0.050/d;20℃厌氧:0.072/d;20℃好氧:0.145/d。其中4个系统由于细胞死亡引起的活性衰减速率分别为:10℃厌氧:0.019/d;10℃好氧:0.017/d;20℃厌氧:0.019/d;20℃好氧:0.03/d,占总活性衰减的比例分别为:35.8%、34%、26.4%、20.7%。在9d饥饿衰减期间,污泥中所含PHA与糖原的量总体呈下降趋势。相同温度下,糖原在厌氧衰减过程中降解速率大于好氧;在同样的厌氧、好氧衰减条件下,温度越高糖原降解速率越快。

污水生物处理系统细菌衰减特征的实验研究

通过衰减速率测定实验和LIVE/DEAD细胞活性染色实验,分析了污水生物处理系统中细菌在好氧环境下的衰减状态.研究发现,细菌的衰减实质上是由生物量减少引起的数量衰减和由细胞活性降低引起的活性衰减两部分构成的.结果表明,硝化系统细菌在衰减过程中由细胞活性降低引起的衰减约占60%,由细胞死亡引起的衰减约占40%;而在异养系统中由活性降低引起的衰减和由细胞死亡引起的衰减分别为80%和20%.

750kV同塔双回输电线路带电作业技术研究

为了确定750kV同塔双回输电线路带电作业安全距离、组合间隙及作业方式等技术要求,结合兰州东—平凉—乾县工程的情况,采用仿真计算和模拟试验的方法,得到了带电作业典型工况下的作业间隙放电特性曲线,以及一回带电一回停电时停电回路上的感应电压和流过接地线的电流。根据计算及试验结果,提出了在海拔0～1000m、1000～2000m、2000～2800m高度下,750kV同塔双回输电线路带电作业等电位和地电位时的最小安全距离,以及进入等电位时的最小组合间隙;提出了一回带电一回停电在停电回路上作业时,应根据停电回路两端接地的情况,采用等电位方式或地电位方式。该研究结果为750kV同塔双回输电线路带电作业的开展提供了技术依据。

一种DNA染料结合聚合酶链反应检测鉴别植物病原细菌死活细胞

建立了一种将DNA染料(EMA)结合PCR的新分析方法,用于有效检测区分植物病原细菌死活细胞.结果表明,当用2.0 mg/L或更高浓度的EMA渗透处理含有106cfu/mL的死细胞菌悬液后再曝光处理10 min,其PCR结果呈阴性,而未经过EMA渗透处理的对应样品PCR结果呈阳性;EMA渗透处理含有适当数量病菌活细胞的种子浸泡液后,更有助于特异性检测混合体系中靶标菌活细胞.分析认为,该方法避免了传统PCR无法区分细菌死活细胞的弊端,是一种快速、灵敏且能有效鉴别病原细菌死活细胞的新方法.

可注射海藻酸钙水凝胶的制备研究

海藻酸钙水凝胶因其良好的生物相容性广泛应用于组织工程支架材料的研究。以海藻酸钠(SA),碳酸钙,葡萄糖酸内酯(GDL)为原料,通过原位相转变制备可注射凝胶,用于软骨组织微创修复材料的研究。测定了单一变量条件下不同海藻酸钠浓度、f值(钙离子与羧基的摩尔比)及n值(葡萄糖酸内酯与钙离子的摩尔比)对海藻酸凝胶力学强度、溶胀率、浸提液pH值等的影响,从而获得各组分最适的配比;另外,通过原位接种软骨细胞,研究了软骨细胞在凝胶中的生长行为。综合海藻酸钙凝胶性能,最终确定海藻酸钠浓度为2.5%、f=0.5及n=0.6为最佳配比;细胞培养结果表明软骨细胞在凝胶中具有较高的活性且维持了其软骨细胞形态,证实了研究制得的海藻酸钙水凝胶是一种优良的可注射软骨组织工程支架材料。

血链菌生物膜形成中死菌/活菌的粘附观察研究

目的 观察血链菌生物膜形成初期的死菌、活菌粘附情况 ,并对其粘附能力进行比较。方法 血链菌混悬于人工唾液 ,其中活菌百分比为 70 %,在模仿体内环境的人工口腔内 ,血链菌悬液以 0 .8ml/min均速滴至唾液覆盖的玻片以形成生物膜 ,应用荧光染色技术观察血链菌生物膜形成中死 /活细菌的初期粘附 ,计算活菌百分比 ;并通过疏水实验、红细胞凝集实验对死 /活菌的粘附特性进行比较。结果 血链菌生物膜形成的 30min ,6 0min ,12 0min ,2 40min ,其中活菌百分比分别为 30 %,5 0 %,6 0 %,70 %;在同一时间血链菌悬液的活菌百分比几乎是恒定的 ,两者相比 :在 30min ,6 0min具有显著性差异 (P <0 .0 5 ) ;在 12 0min ,2 40min无显著性差异 (P>0 .0 5 )。疏水实验、红细胞凝集实验中 ,死菌的疏水性能、红血球凝集能力均强于活菌。结论 血链菌生物膜形成的初期 ,死菌可能比活菌更易粘附于固体表面。

国内外有机果园杂草管理技术研究综述

在有机果园的日常管理中,应摈弃传统农业"根除杂草"的观念,建立"杂草管理"的可持续发展理念。杂草管理对建立和维持一个良好的有机果园尤为重要。本文主要围绕杂草对果园的影响以及如何有效地进行杂草管理展开综述,重点介绍了国内外有机果园普遍采用的杂草管理技术(包括耕作除草、果园覆草、生草覆盖、地膜覆盖、有机除草剂和火焰除草),旨在为有机果农实施杂草管理提供有益借鉴。

焊接残余应力有限元分析技术研究

本文以岭澳核电站控制棒驱动机构(CRDM)耐压壳Ω焊接为例,应用ANSYS有限元生死单元技术模拟焊接流程,并计算焊接后残余应力的分布,绘制残余应力分布曲线并进行计算结果分析。通过本项目的研究,掌握了焊接残余应力的分析技术,达到了研究目的。

EMA-PCR检测金黄色葡萄球菌活菌的方法

根据金黄色葡萄球菌(SA)耐热核酸酶编码基因(nuc基因)设计特异性引物,对试验菌株进行PCR检测,结果显示,金黄色葡萄球菌扩增出大小为480bp的特异性条带,而其他对照菌株未扩增出条带。由于传统PCR技术无法区分样品中的死细菌与活细菌,从而使检测结果往往出现很高的假阳性。本试验利用EMA能穿过死细菌的细胞膜并在光激活的作用下能与基因组DNA共价结合,从而能抑制死菌DNA进行PCR扩增的特性,建立了一种快速、有效的检测金黄色葡萄球菌活菌的EMA-PCR方法,较传统PCR大大提高了检测的准确性和可靠性,该方法检测灵敏度可达15CFU/mL。

杉木人工林树上宿存枯死枝、叶在冠层与在枯枝落叶层分解的比较研究

杉木枯死枝、叶有在树上宿存多年的特性。江西分宜大岗山地区一立地指数 14 ,林龄 13a ,保留密度 2 60 0株·hm- 2 的 1代林 ,树上宿存枯死枝、叶总量达 4 10 8 0kg·hm- 2 ,相当枯枝落叶层生物量的 3 5 4 %。树上宿存枯死枝、叶在冠层的分解速度 ,与置于枯枝落叶层的分解速度的测定结果表明 ,后者比前者快得多。说明在杉木林生长急需养分的速生期和杆材期 ,将树上宿存枯死枝、叶适时地打落于地表 ,使其在枯枝落叶层分解 ,与挂在冠层上的分解相比 ,对林木营养作用不同 ,前者比后者能及时、更多地提供养分。树上宿存枯死枝、叶在林冠下部由低到高呈 4层分布。鉴于各层死亡时间不同 ,经受的分解时间和雨水淋溶的强度也相异 ,其在树冠上的分解速度是分层测定的 ,上下层差异较大。在枯枝落叶层的分解速度也分层测定 ,上下层的差异较小。在枯枝落叶层分解时 ,本实验 1年间枝的各层分解量之和是树冠上分解时的近 2 3倍 ,叶为 5 4倍。在枯枝落叶层分解时 ,1年间各层枝、叶 ,营养元素归还量之和是树冠上分解时的 3 5倍。速生、杆材期 ,适时地将树上宿存枯死枝、叶打落于地表 。