Objective To explore the possibility of using specific antigens for immunodiagnosis of hookworm desease in endemic area. Method Infective third stage larvae of the canine hookworm, Ancylostoma caninum (A. Cani...Objective To explore the possibility of using specific antigens for immunodiagnosis of hookworm desease in endemic area. Method Infective third stage larvae of the canine hookworm, Ancylostoma caninum (A. Caninum) , were prepared as the source of antigen. Enzyme linked immunoelectrotransfer blotting (EITB) was enployed as an immunodiagnostic method. Results Two immunodominant bands of hookworm antigens (42 kDa and 55 kDa) were recognized by the sera of hookworm infected patients (serum dilution 1∶200; antigen centrifuged at 36 000 r/m for 20 minutes, but not by sera from negative controls. Conclusion The 42 kDa and 55 kDa A. caninum antigens might be the specific antigens that could be used for immunodiagnosis of hookworm disease in endemic area.展开更多
文摘Objective To explore the possibility of using specific antigens for immunodiagnosis of hookworm desease in endemic area. Method Infective third stage larvae of the canine hookworm, Ancylostoma caninum (A. Caninum) , were prepared as the source of antigen. Enzyme linked immunoelectrotransfer blotting (EITB) was enployed as an immunodiagnostic method. Results Two immunodominant bands of hookworm antigens (42 kDa and 55 kDa) were recognized by the sera of hookworm infected patients (serum dilution 1∶200; antigen centrifuged at 36 000 r/m for 20 minutes, but not by sera from negative controls. Conclusion The 42 kDa and 55 kDa A. caninum antigens might be the specific antigens that could be used for immunodiagnosis of hookworm disease in endemic area.
