Pharmacological, laser scanning confocal microscopic （LSCM）, and spectrophotographic approaches were used to study the roles of hydrogen sulfide （H2S） and nitric oxide （NO） in signaling transduction of stomatal ...Pharmacological, laser scanning confocal microscopic （LSCM）, and spectrophotographic approaches were used to study the roles of hydrogen sulfide （H2S） and nitric oxide （NO） in signaling transduction of stomatal movement in response to ethylene in Viciafaba L. Ethylene treatment resulted in the dose-dependent stomatal closure under light, and this effect was blocked by the inhibitors of H2S biosynthesis in V. faba L. Additionally, ethylene induces H2S generation and increases L-/D-cysteine desulfhydrase （pyridoxalphosphate-dependent enzyme） activity in leaves of V. faba L. Inhibitors of H2S biosynthesis have no effect on the ethylene-induced stomatal closure, NO accumulation, and nitrate reductase （NR） activity in guard cells or leaves of II. faba L. Moreover, the ethylene-induced increase of H2S levels and L-/D- cysteine desulfhydrase activity declined when NO generation was inhibited. Therefore, we conclude that H2S and NO probably are involved in the signal transduction pathway of ethylene-induced stomatal closure. H2S may represent a novel component downstream of NO in the ethylene-induced stomatal movement in V. faba L.展开更多
To analyze the nutritional composition of faba bean(Vicia faba L.) seed, estimation models were developed for protein, starch, oil, and total polyphenol using near infrared spectroscopy(NIRS). Two hundred and forty-fo...To analyze the nutritional composition of faba bean(Vicia faba L.) seed, estimation models were developed for protein, starch, oil, and total polyphenol using near infrared spectroscopy(NIRS). Two hundred and forty-four samples from twelve producing regions were measured in both milled powder and intact seed forms. Partial least squares(PLS) regression was applied for model development. The model based on ground seed powder was generally superior to that based on the intact seed. The optimal seed powder-based models for protein, starch, and total polyphenol had coefficients of correlation(r2) of 0.97, 0.93 and 0.89, respectively. The relationship between nutrient contents and twelve producing areas was determined by two-step cluster analysis. Three distinct groupings were obtained with region-constituent features, i.e., Group 1 of high oil, Group 2 of high protein, and Group 3 of high starch as well as total polyphenol. The clustering accuracy was 79.5%. Moreover, the nutrition contents were affected by seeding date, longitude, latitude, and altitude of plant location. Cluster analysis revealed that the differences in the seed were strongly influenced by geographical factors.展开更多
Faba bean is suffered with many biotic and abiotic factors. Chocolate spot disease, caused by Botrytis fabae is one of the biotic factors limiting yields of this crop resulting in yield losses up to 68% in Ethiopia. T...Faba bean is suffered with many biotic and abiotic factors. Chocolate spot disease, caused by Botrytis fabae is one of the biotic factors limiting yields of this crop resulting in yield losses up to 68% in Ethiopia. The experiment was conducted during 2020/2021 cropping season at Tach Gayint district, Ethiopia to determine the integration of faba bean varieties and fungicide rates on reducing chocolate spot disease. The experiment consisted of 12 treatments, viz. three faba bean varieties and four rate of Mancozeb fungicide in factorial arrangement. The experiment was laid out as randomized complete block design (RCBD) with three replications. Data was collected and analyzed. Results indicated that, disease incidence was reached at maximum percentage in all treatments on the last dates of assessment. But treatments were significantly difference in severity level. The least disease severity was recorded from varieties treated by 3.5 kg/ha of Mancozeb 80% WP with mean values Walki (12.7%), and (18.1% and 20.8%) on Gora and local variety respectively at the final dates of disease assessment. Similarly, the reduced AUDPC was also recorded from varieties treated by 3.5 kg/ha of Mancozeb 80% WP with mean values Walki (371.8% unit/day) and (539% and 686.4% days) on varieties Gora, and Local respectively. Whereas the maximum disease severity and AUDPC were obtained from unsprayed plots. Based on the results obtained, variety Walki treated with Mancozeb 80% WP at rate of 2.5 kg/ha and 3.5 kg/ha were effective to reduce the effect of chocolate spot disease for the study area.展开更多
Faba bean (Vicia faba L) seeds are an important source of plant protein for humans and animals. A total of 15,697 Differentially Expressed Genes (DEGs) with pathway annotation were discovered in RNA-Seq of the faba be...Faba bean (Vicia faba L) seeds are an important source of plant protein for humans and animals. A total of 15,697 Differentially Expressed Genes (DEGs) with pathway annotation were discovered in RNA-Seq of the faba bean seeds. A total of 75 significant KEGG pathways abundance were discovered and 9 pathways were conserved within all genotypes. 41 significant pathways were found to be partially conserved within comparisons of 2 to 6 pairs of genotypes and 25 significant pathways were unique to single pairs of genotypes. There were 8 specific significant pathways discovered related to the faba bean seed Hydration Capacity trait and 9 specific significant pathways discovered related to the PSbMV seeds staining trait. The DEGs demonstrated the genetic distance between these varieties was confirmed by the breeding pedigree selection information and a PCA graph clearly illustrated the genetic distance within these genotypes.展开更多
Twelve Australian grown faba bean genotypes with seed colour ranging from white, different shades of buff/beige, green, red and purple, were extracted using 80% methanol (v/v) or 70% acetone (v/v). The findings showed...Twelve Australian grown faba bean genotypes with seed colour ranging from white, different shades of buff/beige, green, red and purple, were extracted using 80% methanol (v/v) or 70% acetone (v/v). The findings showed that commonly consumed buff-coloured genotypes have comparable phenolic contents and antioxidant activities to those with exotic seed coat colour (red, green and purple). In general, the extraction yield for methanol and acetone extracts of faba bean genotypes were similar. However, the acetone extracts of faba bean varieties with coloured seed coats exhibited higher antioxidant activities than their methanol extracts counterparts. Phenolic compounds and antioxidant activities of the genotypes were compared in an array of chemical-based assays and profiled using an on-line high-performance liquid chromatography-post column derivatization (HPLC-PCD) system. The total phenolic content (TPC) and total flavonoid content (TFC) of acetone extracts were approximately twice those of methanol extracts. The acetone extracts contained six times higher levels of di(phenyl)-(2,4,6-trinitrophenyl) imi- noazanium radical scavenging activity (DPPH), six times higher Total Equivalent Antioxidant Capacity (TEAC) and two times higher Ferric Reducing Antioxidant Power (FRAP) than those of methanol extracts. In general, the methanol extracts of white-coloured faba bean genotype exhibited comparable phenolic contents and antioxidant activities to varieties with coloured seed coats. However, the TPC, TFC, DPPH, TEAC and FRAP of acetone extracts from white-coloured genotype were 2 - 4, 1 - 2, 5 - 9, 2 - 3 and 1 - 2 times lower than those of faba bean varieties with coloured seed coats. HPLC-PCD analyses showed substantial antioxidant responses, represented as a dense “hump” of peaks in the HPLC chromatograms of acetone extracts from coloured-genotypes. This “hump” was not detected in the chromatograms of white-genotype acetone extracts, or in chromatograms of methanol extracts regardless of genotype or seed coat colour. All coloured-beans had higher phenolic contents and antioxidant activities than the white-genotype. Hydroxybenzoic acids/flavanols, hydroxycinnamic acids and flavonols were dominant in coloured beans. The findings suggest potentials in selecting commonly consumed buff-coloured faba bean genotypes within the breeding programs for enhanced levels of phenolic compounds and antioxidant activities, potentially increasing their health-promoting properties. And, there might also be potentials in developing faba bean extracts for pharmaceutical or natural medicines.展开更多
基金supported by the National Natural Science Foundation of China (30970288 and 31170237)the National Science Foundation of Shandong Province, China(ZR2010CM024)the Foundation of State Key Laboratory of Plant Physiology and Biochemistry, China(SKLPPBKF11001)
文摘Pharmacological, laser scanning confocal microscopic （LSCM）, and spectrophotographic approaches were used to study the roles of hydrogen sulfide （H2S） and nitric oxide （NO） in signaling transduction of stomatal movement in response to ethylene in Viciafaba L. Ethylene treatment resulted in the dose-dependent stomatal closure under light, and this effect was blocked by the inhibitors of H2S biosynthesis in V. faba L. Additionally, ethylene induces H2S generation and increases L-/D-cysteine desulfhydrase （pyridoxalphosphate-dependent enzyme） activity in leaves of V. faba L. Inhibitors of H2S biosynthesis have no effect on the ethylene-induced stomatal closure, NO accumulation, and nitrate reductase （NR） activity in guard cells or leaves of II. faba L. Moreover, the ethylene-induced increase of H2S levels and L-/D- cysteine desulfhydrase activity declined when NO generation was inhibited. Therefore, we conclude that H2S and NO probably are involved in the signal transduction pathway of ethylene-induced stomatal closure. H2S may represent a novel component downstream of NO in the ethylene-induced stomatal movement in V. faba L.
基金financed by the Modern Agro-industry Technology Research System (nycyty-018: Guixing Ren)the National Infrastructure of Crop Germplasm Resources and the Sci & Tech Innovation Program of CAAS
文摘To analyze the nutritional composition of faba bean(Vicia faba L.) seed, estimation models were developed for protein, starch, oil, and total polyphenol using near infrared spectroscopy(NIRS). Two hundred and forty-four samples from twelve producing regions were measured in both milled powder and intact seed forms. Partial least squares(PLS) regression was applied for model development. The model based on ground seed powder was generally superior to that based on the intact seed. The optimal seed powder-based models for protein, starch, and total polyphenol had coefficients of correlation(r2) of 0.97, 0.93 and 0.89, respectively. The relationship between nutrient contents and twelve producing areas was determined by two-step cluster analysis. Three distinct groupings were obtained with region-constituent features, i.e., Group 1 of high oil, Group 2 of high protein, and Group 3 of high starch as well as total polyphenol. The clustering accuracy was 79.5%. Moreover, the nutrition contents were affected by seeding date, longitude, latitude, and altitude of plant location. Cluster analysis revealed that the differences in the seed were strongly influenced by geographical factors.
文摘Faba bean is suffered with many biotic and abiotic factors. Chocolate spot disease, caused by Botrytis fabae is one of the biotic factors limiting yields of this crop resulting in yield losses up to 68% in Ethiopia. The experiment was conducted during 2020/2021 cropping season at Tach Gayint district, Ethiopia to determine the integration of faba bean varieties and fungicide rates on reducing chocolate spot disease. The experiment consisted of 12 treatments, viz. three faba bean varieties and four rate of Mancozeb fungicide in factorial arrangement. The experiment was laid out as randomized complete block design (RCBD) with three replications. Data was collected and analyzed. Results indicated that, disease incidence was reached at maximum percentage in all treatments on the last dates of assessment. But treatments were significantly difference in severity level. The least disease severity was recorded from varieties treated by 3.5 kg/ha of Mancozeb 80% WP with mean values Walki (12.7%), and (18.1% and 20.8%) on Gora and local variety respectively at the final dates of disease assessment. Similarly, the reduced AUDPC was also recorded from varieties treated by 3.5 kg/ha of Mancozeb 80% WP with mean values Walki (371.8% unit/day) and (539% and 686.4% days) on varieties Gora, and Local respectively. Whereas the maximum disease severity and AUDPC were obtained from unsprayed plots. Based on the results obtained, variety Walki treated with Mancozeb 80% WP at rate of 2.5 kg/ha and 3.5 kg/ha were effective to reduce the effect of chocolate spot disease for the study area.
文摘Faba bean (Vicia faba L) seeds are an important source of plant protein for humans and animals. A total of 15,697 Differentially Expressed Genes (DEGs) with pathway annotation were discovered in RNA-Seq of the faba bean seeds. A total of 75 significant KEGG pathways abundance were discovered and 9 pathways were conserved within all genotypes. 41 significant pathways were found to be partially conserved within comparisons of 2 to 6 pairs of genotypes and 25 significant pathways were unique to single pairs of genotypes. There were 8 specific significant pathways discovered related to the faba bean seed Hydration Capacity trait and 9 specific significant pathways discovered related to the PSbMV seeds staining trait. The DEGs demonstrated the genetic distance between these varieties was confirmed by the breeding pedigree selection information and a PCA graph clearly illustrated the genetic distance within these genotypes.
文摘Twelve Australian grown faba bean genotypes with seed colour ranging from white, different shades of buff/beige, green, red and purple, were extracted using 80% methanol (v/v) or 70% acetone (v/v). The findings showed that commonly consumed buff-coloured genotypes have comparable phenolic contents and antioxidant activities to those with exotic seed coat colour (red, green and purple). In general, the extraction yield for methanol and acetone extracts of faba bean genotypes were similar. However, the acetone extracts of faba bean varieties with coloured seed coats exhibited higher antioxidant activities than their methanol extracts counterparts. Phenolic compounds and antioxidant activities of the genotypes were compared in an array of chemical-based assays and profiled using an on-line high-performance liquid chromatography-post column derivatization (HPLC-PCD) system. The total phenolic content (TPC) and total flavonoid content (TFC) of acetone extracts were approximately twice those of methanol extracts. The acetone extracts contained six times higher levels of di(phenyl)-(2,4,6-trinitrophenyl) imi- noazanium radical scavenging activity (DPPH), six times higher Total Equivalent Antioxidant Capacity (TEAC) and two times higher Ferric Reducing Antioxidant Power (FRAP) than those of methanol extracts. In general, the methanol extracts of white-coloured faba bean genotype exhibited comparable phenolic contents and antioxidant activities to varieties with coloured seed coats. However, the TPC, TFC, DPPH, TEAC and FRAP of acetone extracts from white-coloured genotype were 2 - 4, 1 - 2, 5 - 9, 2 - 3 and 1 - 2 times lower than those of faba bean varieties with coloured seed coats. HPLC-PCD analyses showed substantial antioxidant responses, represented as a dense “hump” of peaks in the HPLC chromatograms of acetone extracts from coloured-genotypes. This “hump” was not detected in the chromatograms of white-genotype acetone extracts, or in chromatograms of methanol extracts regardless of genotype or seed coat colour. All coloured-beans had higher phenolic contents and antioxidant activities than the white-genotype. Hydroxybenzoic acids/flavanols, hydroxycinnamic acids and flavonols were dominant in coloured beans. The findings suggest potentials in selecting commonly consumed buff-coloured faba bean genotypes within the breeding programs for enhanced levels of phenolic compounds and antioxidant activities, potentially increasing their health-promoting properties. And, there might also be potentials in developing faba bean extracts for pharmaceutical or natural medicines.
