Trichloroethylene （TCE） is a major pollutant that affects both occupational and general environments. The liver is an important target organ of TCEE. Substantial efforts and remarkable progress into understanding TC...Trichloroethylene （TCE） is a major pollutant that affects both occupational and general environments. The liver is an important target organ of TCEE. Substantial efforts and remarkable progress into understanding TCE cytotoxicity have been made in cultured liver cells. However, the molecular mechanisms by which TCE induces hepatotoxicity are not well understood. SET （also known as protein phosphatase 2A inhibitor, 12PP2A, or template-activating factor-I, TAF-D is a nuclear protein that regulates histone modification, gene transcription, DNA replication, nucleosome assembly,展开更多
[Objectives] To study the protection of compatibility of Saikosapon d and Baicalin on carbon tetrachloride( CCl_4) injured L-02 cells. [Methods] Normal human hepatocyte cell line L-02 cells were cultured in vitro,and ...[Objectives] To study the protection of compatibility of Saikosapon d and Baicalin on carbon tetrachloride( CCl_4) injured L-02 cells. [Methods] Normal human hepatocyte cell line L-02 cells were cultured in vitro,and CCl_4 was used to induce hepatocellular injury. Interventions were carried out with Saikosaponin d and Baicalin at different dosage. The proliferation of L-02 cells in each group was determined by methylthiazolyl tetrazolium( MTT) assay; the levels of AST and ALT in the culture supernatants were detected by enzyme-linked immunosorbent assay( ELISA); the expressions of TLR4 and NFκBp65 proteins in each group were determined by immunohistochemistry.[Results] In the CCl_4 injured group,the proliferation of L-02 cells was significantly declined,the levels of AST and ALT in cell culture medium were significantly increased,and the expressions of TLR4 and NFκBp65 in L-02 cells were increased; after the intervention of Saikosaponin d and Baicalin,1. 75 μg/mL group and 1. 5 μg/mL group had an effect of promoting the proliferation of L-02 cells and could reduce the levels of AST and ALT in the cell culture medium,and TLR4 and NFκBp65 proteins in L-02 cells also had a certain inhibitory effect. [Conclusions] The compatibility of Saikosapon d and Baicalin has a certain protective effect on CCl_4 injured L-02 cells. The protection mechanism may be related with its down-regulating TLR4-NFκB signaling pathway and reducing the inflammation.展开更多
目的观察葛黄颗粒含药血清对乙醇损伤的人肝细胞L-02修复作用,并探讨其可能机制。方法 (1)将L-02细胞分为A、B、C、D、E、F组。除F组外,其余各组均加入乙醇培养基培养24 h制备肝细胞损伤模型,造模后吸走培养液。D、F组加入空白血清(10%...目的观察葛黄颗粒含药血清对乙醇损伤的人肝细胞L-02修复作用,并探讨其可能机制。方法 (1)将L-02细胞分为A、B、C、D、E、F组。除F组外,其余各组均加入乙醇培养基培养24 h制备肝细胞损伤模型,造模后吸走培养液。D、F组加入空白血清(10%正常大鼠血清),A组加入1.25%葛黄颗粒含药血清+8.75%正常大鼠血清,B组加入2.5%葛黄颗粒含药血清+7.5%正常大鼠血清,C组加入5%葛黄颗粒含药血清+5%正常大鼠血清,E组加入5%美他多辛含药血清+5%正常大鼠血清,继续培养6、12、24 h。比较各组细胞上清液谷草转氨酶(AST)、乳酸脱氢酶(LDH)活力。(2)将L-02细胞按2×104个/孔接种于6孔板中,将其分为a、b、c、d组。除d组外,其余各组均加入乙醇制备肝细胞损伤模型,造模后吸走培养液。b、d组加入空白血清,a组加入5%葛黄颗粒含药血清,c组加入5%美他多辛含药血清,继续培养24 h。比较各组丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-px)、细胞活性氧(ROS)、线粒体膜电位(MMP)、三磷酸腺苷(ATP)。结果与F组比较,D组各时点AST、LDH升高;与D组比较,B组24 h AST、LDH降低,C、E组12 h和24 h的AST、LDH降低;与A组比较,B组24 h AST降低,C组24 h AST及12 h LDH降低,E组12、24 h AST和LDH降低;与B组比较,C、E组12 h AST、LDH降低;与同组6 h比较,C组24 h AST降低,12、24 h LDH降低;与同组12 h比较,B、C、E组24 h AST降低,C组24 h LDH降低;P均<0.05。与d组比较,b组SOD、GSH-px、MMP、ATP降低,MDA、ROS升高(P均<0.05);与b组比较,a组和c组SOD、GSH-px、MMP、ATP升高,MDA、ROS降低(P均<0.05)。结论葛黄颗粒含药血清对乙醇致L-02细胞损伤有一定修复作用,其机制可能是调整细胞内氧化应激水平和线粒体功能。展开更多
Objective To investigate the effects of sodium arsenite（NaAsO2）on the expression of microRNA-191（miR-191）and tissue inhibitor of metalloproteinase 3（TIMP-3）in human normal hepatic cells（L-02 cells）.Methods L-0...Objective To investigate the effects of sodium arsenite（NaAsO2）on the expression of microRNA-191（miR-191）and tissue inhibitor of metalloproteinase 3（TIMP-3）in human normal hepatic cells（L-02 cells）.Methods L-02 cells were exposed to different doses of Na2As O2[0（control group）,5,25,50 and 75μmol/L]展开更多
基金supported by NSFC (the National Natural Science Foundation of China) [81273126, 30972454]the Key Project of Guangdong Natural Science Foundation [S2012020010903]+2 种基金the Project of Shenzhen Basic Research Plan [JCYJ20120616 154222545]the Upgrade Scheme of Shenzhen Municipal Key Laboratory [CXB201005260068A]Medical Scientific Research Foundation of Guangdong Province (A2012577)
文摘Trichloroethylene （TCE） is a major pollutant that affects both occupational and general environments. The liver is an important target organ of TCEE. Substantial efforts and remarkable progress into understanding TCE cytotoxicity have been made in cultured liver cells. However, the molecular mechanisms by which TCE induces hepatotoxicity are not well understood. SET （also known as protein phosphatase 2A inhibitor, 12PP2A, or template-activating factor-I, TAF-D is a nuclear protein that regulates histone modification, gene transcription, DNA replication, nucleosome assembly,
基金Supported by Project of Shaanxi Administration of Traditional Chinese Medicine(zy06)Special Project of Education Department of Shaanxi Provincial Government(12JK1016)Program of Shaanxi Provincial Department of Science and Technology(2013jk4023)
文摘[Objectives] To study the protection of compatibility of Saikosapon d and Baicalin on carbon tetrachloride( CCl_4) injured L-02 cells. [Methods] Normal human hepatocyte cell line L-02 cells were cultured in vitro,and CCl_4 was used to induce hepatocellular injury. Interventions were carried out with Saikosaponin d and Baicalin at different dosage. The proliferation of L-02 cells in each group was determined by methylthiazolyl tetrazolium( MTT) assay; the levels of AST and ALT in the culture supernatants were detected by enzyme-linked immunosorbent assay( ELISA); the expressions of TLR4 and NFκBp65 proteins in each group were determined by immunohistochemistry.[Results] In the CCl_4 injured group,the proliferation of L-02 cells was significantly declined,the levels of AST and ALT in cell culture medium were significantly increased,and the expressions of TLR4 and NFκBp65 in L-02 cells were increased; after the intervention of Saikosaponin d and Baicalin,1. 75 μg/mL group and 1. 5 μg/mL group had an effect of promoting the proliferation of L-02 cells and could reduce the levels of AST and ALT in the cell culture medium,and TLR4 and NFκBp65 proteins in L-02 cells also had a certain inhibitory effect. [Conclusions] The compatibility of Saikosapon d and Baicalin has a certain protective effect on CCl_4 injured L-02 cells. The protection mechanism may be related with its down-regulating TLR4-NFκB signaling pathway and reducing the inflammation.
文摘目的观察葛黄颗粒含药血清对乙醇损伤的人肝细胞L-02修复作用,并探讨其可能机制。方法 (1)将L-02细胞分为A、B、C、D、E、F组。除F组外,其余各组均加入乙醇培养基培养24 h制备肝细胞损伤模型,造模后吸走培养液。D、F组加入空白血清(10%正常大鼠血清),A组加入1.25%葛黄颗粒含药血清+8.75%正常大鼠血清,B组加入2.5%葛黄颗粒含药血清+7.5%正常大鼠血清,C组加入5%葛黄颗粒含药血清+5%正常大鼠血清,E组加入5%美他多辛含药血清+5%正常大鼠血清,继续培养6、12、24 h。比较各组细胞上清液谷草转氨酶(AST)、乳酸脱氢酶(LDH)活力。(2)将L-02细胞按2×104个/孔接种于6孔板中,将其分为a、b、c、d组。除d组外,其余各组均加入乙醇制备肝细胞损伤模型,造模后吸走培养液。b、d组加入空白血清,a组加入5%葛黄颗粒含药血清,c组加入5%美他多辛含药血清,继续培养24 h。比较各组丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-px)、细胞活性氧(ROS)、线粒体膜电位(MMP)、三磷酸腺苷(ATP)。结果与F组比较,D组各时点AST、LDH升高;与D组比较,B组24 h AST、LDH降低,C、E组12 h和24 h的AST、LDH降低;与A组比较,B组24 h AST降低,C组24 h AST及12 h LDH降低,E组12、24 h AST和LDH降低;与B组比较,C、E组12 h AST、LDH降低;与同组6 h比较,C组24 h AST降低,12、24 h LDH降低;与同组12 h比较,B、C、E组24 h AST降低,C组24 h LDH降低;P均<0.05。与d组比较,b组SOD、GSH-px、MMP、ATP降低,MDA、ROS升高(P均<0.05);与b组比较,a组和c组SOD、GSH-px、MMP、ATP升高,MDA、ROS降低(P均<0.05)。结论葛黄颗粒含药血清对乙醇致L-02细胞损伤有一定修复作用,其机制可能是调整细胞内氧化应激水平和线粒体功能。
文摘Objective To investigate the effects of sodium arsenite（NaAsO2）on the expression of microRNA-191（miR-191）and tissue inhibitor of metalloproteinase 3（TIMP-3）in human normal hepatic cells（L-02 cells）.Methods L-02 cells were exposed to different doses of Na2As O2[0（control group）,5,25,50 and 75μmol/L]
