The speciation and distribution of Gd(III) in human interstitial fluid was studied by computer simulation. Meantime artificial neural network was applied to the estimation of log ?values of complexes. The results sh...The speciation and distribution of Gd(III) in human interstitial fluid was studied by computer simulation. Meantime artificial neural network was applied to the estimation of log ?values of complexes. The results show that the precipitate species, GdPO4 and Gd2(CO3)3, are the predominant species. Among soluble species, the free Gd(III), [Gd(HSA)] , [Gd(Ox)] and then the ternary complexes of Gd(III) with citrate are main species and [Gd3(OH)4] becomes the predominant species at the Gd(III) total concentration of 2.2?0-2mol/L.展开更多
A multi-phase model of Pr(III) speciation in human interstitial fluid was constructed and insoluble Pr(III) speciation was studied. When the total concentration of Pr(III) is below 8.401E-10 mol/L, soluble Pr(III) sp...A multi-phase model of Pr(III) speciation in human interstitial fluid was constructed and insoluble Pr(III) speciation was studied. When the total concentration of Pr(III) is below 8.401E-10 mol/L, soluble Pr(III) species are main species. With rising the total concentration of Pr(III), Pr(III) is firstly bound to phosphate to form precipitate of PrPO4, then bound to carbonate and another precipitate of Pr2(CO3)3 was obtained. When the total concentration is between 1.583E-9 mol/L and 4.000E-3 mol/L, the insoluble species are predominant Pr(III) species.展开更多
This paper deals with mathematical study of diffusive fluid transport and distri- bution in human dermal parts. It accounts the intracellular fluid which continuously flows through the skin shells in order to maintain...This paper deals with mathematical study of diffusive fluid transport and distri- bution in human dermal parts. It accounts the intracellular fluid which continuously flows through the skin shells in order to maintain fluid balance within the body. A mathematical model is envisaged for this process and the finite element method （FEM） is employed to calculate the concentration of the fluid at different skin layers. This estimation is analyzed in relation with other parameters of the tissue medium and the atmosphere.展开更多
Male infertility is a major public health issue predominantly caused by defects in germ cell development. In the past, studies on the genetic regulation of spermatogenesis as well as on negative environmental impacts ...Male infertility is a major public health issue predominantly caused by defects in germ cell development. In the past, studies on the genetic regulation of spermatogenesis as well as on negative environmental impacts have been hampered by the fact that human germ cell development is intractable to direct analysis in vivo. Compared with model organisms including mice, there are fundamental differences in the molecular processes of human germ cell development. Therefore, an in vitro model mimicking human sperm formation would be an extremely valuable research tool. In the recent past, both human embryonic stem （ES） cells and induced pluripotent stem （iPS） cells have been reported to harbour the potential to differentiate into primordial germ cells and gametes. We here discuss the possibility to use human amniotic fluid stem （AFS） ceils as a biological model. Since their discovery in 2003, AFS cells have been characterized to differentiate into cells of all three germ layers, to be genomically stable, to have a high proliferative potential and to be non-tumourigenic. In addition, AFS cells are not subject of ethical concerns. In contrast to iPS cells, AFSs cells do not need ectopic induction of pluripotency, which is often associated with only imperfectly cleared epigenetic memory of the source cells. Since AFS cells can be derived from amniocentesis with disease-causing mutations and can be transfected with high efficiency, they could be used in probing gene functions for spermatogenesis and in screening for male reproductive toxicity.展开更多
A facility and method for109 Cd radionuclide-induced energy-dispersive X-ray fluorescent(EDXRF) were developed to determine the Fe,Zn,Br,Rb,and Sr concentrations in the specimens of human prostatic fluid.Specimens of ...A facility and method for109 Cd radionuclide-induced energy-dispersive X-ray fluorescent(EDXRF) were developed to determine the Fe,Zn,Br,Rb,and Sr concentrations in the specimens of human prostatic fluid.Specimens of expressed prostatic fluid were obtained from 51 men(mean age 51 years,range 18–82 years) with apparently normal prostates using standard rectal massage procedure.Mean values(M ± SEL) for concentration of trace elements(mg L^(-1)) in human prostate fluid were:Fe 9.04 ±1.21,Zn 573 ± 35,Br 3.58 ± 0.59,Rb 1.10 ± 0.08,and Sr B 0.76.It was shown that the results of trace element analysis in the micro-samples(20 l L) are sufficiently representative for assessment of the Fe,Zn,Br,and Rb concentration in the prostate fluid.The facility for109 Cd radionuclide-induced EDXRF is comparatively compact and can be located in close proximity to the site of carrying out the massage procedure.The means of Zn and Rb concentration obtained for prostate fluid agree well with median of reported means.For the first time,the Fe and Br concentrations,as well as the upper limit of the Sr concentration,were determined in the human prostate fluid.展开更多
Objective:To explore the serodiagnosis of hydatid cyst in human using different antigens of sheep(hydatid fluid,somatic and Lxcretory/secretory antigens of protoscolexi by ELISA and compares this result with commercia...Objective:To explore the serodiagnosis of hydatid cyst in human using different antigens of sheep(hydatid fluid,somatic and Lxcretory/secretory antigens of protoscolexi by ELISA and compares this result with commercial human ELISA kit.Methods:one hundred blood samples from patients with history of severe abdominal pain and cosinophilia were obtained.Ten serum samples were obtained from surgically and pathologically confirmed cystic echinococcosis patients from Mashhad university hospital as positive control and 5 serum samples from infant under one year old as negative control.Blood samples were centrifuged at 3000xg at 20℃for 15 min and sera were stored at-20℃.First,these samples were tested for the presence of antibody by commercial human ELISA.Then.ELISA was developed on microplates coated with hydatid fluid,Somatic and Excretory/secretory antigens of protoscolex of sheep.Results:The results of this study as analyzed by Kappa test showed that,hydatid fluid antigen could be used as a precise source of detection in indirect ELISA test.Conclusions:Hydatid fluid in comparison with Excretory-secretory and somatic antigens showed more compatibility agreement in kappa test which can be used for further studies in development of any ELISA test for diagnosis of human hydatidosis.展开更多
Age-related NADH oxidase (arNOX = ENOX3) proteins are superoxide-generating cell surface oxidases that increase in activity with age beginning at about 30 y. A soluble and truncated exfoliated form of the activity is ...Age-related NADH oxidase (arNOX = ENOX3) proteins are superoxide-generating cell surface oxidases that increase in activity with age beginning at about 30 y. A soluble and truncated exfoliated form of the activity is present in blood and other body fluids. The activity was purified to apparent homogeneity from human urine and resolved by 2-D gel electrophoresis into a series of 24 to 32 kDa components of low isoelectric point. The purified proteins were resistant both to N-terminal sequencing and trypsin cleavage. Cleavage with pepsin revealed peptides corresponding to the TM9 family of transmembrane proteins. Peptide antisera raised to all five members of the human TM9 family sequentially blocked the arNOX activity of human saliva and sera. The soluble truncated N-terminus of the human homolog TM9SF4 was expressed in bacteria. The recombinant protein was characterized biochemically and exhibited ar-NOX activity. The findings identify five arNOX isoforms each of which correspond to one of the five known TM9 family members. The exfoliated soluble arNOX forms are derived from the 24 to 32 kDa N-termini exposed to the cell’s exterior at the cell surface. Each of the shed forms contain putative functional motifs characteristic of ECTO-NOX (ENOX) proteins despite only minimal sequence identity. Our findings identify arNOX as having functional characteristics of ENOX proteins and the TM9 superfamily of proteins as the genetic origins of the five known arNOX isoforms present in human sera, plasma and other body fluids1.展开更多
A three-dimensional computational fluid dynamics (CFD) simulation of the physiological pulsatile blood flow in the human aortic arch and its three branches has been conducted by using commercial software StarCD. The b...A three-dimensional computational fluid dynamics (CFD) simulation of the physiological pulsatile blood flow in the human aortic arch and its three branches has been conducted by using commercial software StarCD. The blood flow, of a peak Reynolds number of 3289 and a Womersley parameter of 16.44, was simulated in a rigid aorta geometry that was built by computer aided design (CAD) reconstruction method based on autopsy data of a female adult. The purpose of this work is to further the understanding of the complex nature of aorta flow, therefore it mainly focuses on analysis of the spatial and temporal distributions of velocities and wall shear stresses. The results, illustrated by 3D visualization pictures and 2D graphs of the primary velocity profiles, wall shear stress and pressure distributions, as well as the secondary flow patterns, are in good agreement with those of other experimental and computational works. The distributions of pressure and wall shear stress support the correlation between high and low shear stresses and pressures and the atherosclerotic lesions.展开更多
The present theoretical assessment deals with the peristaltic-ciliary trans- port of a developing embryo within a fallopian tubal fluid in the human fallopian tube. A mathematical model of peristalsis-cilia induced fl...The present theoretical assessment deals with the peristaltic-ciliary trans- port of a developing embryo within a fallopian tubal fluid in the human fallopian tube. A mathematical model of peristalsis-cilia induced flow of a linearly viscous fluid within a fallopian tubal fluid in a finite two-dimensional narrow tube is developed. The lubrica- tion approximation theory is used to solve the resulting partial differential equation. The expressions for axial and radial velocities, pressure gradient, stream function, volume flow rate, and time mean volume flow rate are derived. Numerical integration is performed for the appropriate residue time over the wavelength and the pressure difference over the wavelength. Moreover, the plots of axial velocity, the appropriate residue time over wavelength, the vector, the pressure difference over wavelength, and the streamlines are displayed and discussed for emerging parameters and constants. Salient features of the pumping characteristics and the trapping phenomenon are discussed in detail. Yhrther- more, a comparison between the peristaltic flow and the peristaltic-ciliary flow is made as the special case. Relevance of the current results to the transport of a developing embryo within a fallopian tubal fluid from ampulla to the intramural in the fallopian tube is also explored. It reveals the fact that cilia along with peristalsis helps to complete the required mitotic divisions while transporting the developing embryo within a fallopian tubal fluid in the human fallopian tube.展开更多
基金financial support of this work (Project Nos. 29890280 29971029).
文摘The speciation and distribution of Gd(III) in human interstitial fluid was studied by computer simulation. Meantime artificial neural network was applied to the estimation of log ?values of complexes. The results show that the precipitate species, GdPO4 and Gd2(CO3)3, are the predominant species. Among soluble species, the free Gd(III), [Gd(HSA)] , [Gd(Ox)] and then the ternary complexes of Gd(III) with citrate are main species and [Gd3(OH)4] becomes the predominant species at the Gd(III) total concentration of 2.2?0-2mol/L.
基金We thank the NNSFC for financial support of this work (Project Nos. 29890280, 29971029).
文摘A multi-phase model of Pr(III) speciation in human interstitial fluid was constructed and insoluble Pr(III) speciation was studied. When the total concentration of Pr(III) is below 8.401E-10 mol/L, soluble Pr(III) species are main species. With rising the total concentration of Pr(III), Pr(III) is firstly bound to phosphate to form precipitate of PrPO4, then bound to carbonate and another precipitate of Pr2(CO3)3 was obtained. When the total concentration is between 1.583E-9 mol/L and 4.000E-3 mol/L, the insoluble species are predominant Pr(III) species.
文摘This paper deals with mathematical study of diffusive fluid transport and distri- bution in human dermal parts. It accounts the intracellular fluid which continuously flows through the skin shells in order to maintain fluid balance within the body. A mathematical model is envisaged for this process and the finite element method （FEM） is employed to calculate the concentration of the fluid at different skin layers. This estimation is analyzed in relation with other parameters of the tissue medium and the atmosphere.
文摘Male infertility is a major public health issue predominantly caused by defects in germ cell development. In the past, studies on the genetic regulation of spermatogenesis as well as on negative environmental impacts have been hampered by the fact that human germ cell development is intractable to direct analysis in vivo. Compared with model organisms including mice, there are fundamental differences in the molecular processes of human germ cell development. Therefore, an in vitro model mimicking human sperm formation would be an extremely valuable research tool. In the recent past, both human embryonic stem （ES） cells and induced pluripotent stem （iPS） cells have been reported to harbour the potential to differentiate into primordial germ cells and gametes. We here discuss the possibility to use human amniotic fluid stem （AFS） ceils as a biological model. Since their discovery in 2003, AFS cells have been characterized to differentiate into cells of all three germ layers, to be genomically stable, to have a high proliferative potential and to be non-tumourigenic. In addition, AFS cells are not subject of ethical concerns. In contrast to iPS cells, AFSs cells do not need ectopic induction of pluripotency, which is often associated with only imperfectly cleared epigenetic memory of the source cells. Since AFS cells can be derived from amniocentesis with disease-causing mutations and can be transfected with high efficiency, they could be used in probing gene functions for spermatogenesis and in screening for male reproductive toxicity.
文摘A facility and method for109 Cd radionuclide-induced energy-dispersive X-ray fluorescent(EDXRF) were developed to determine the Fe,Zn,Br,Rb,and Sr concentrations in the specimens of human prostatic fluid.Specimens of expressed prostatic fluid were obtained from 51 men(mean age 51 years,range 18–82 years) with apparently normal prostates using standard rectal massage procedure.Mean values(M ± SEL) for concentration of trace elements(mg L^(-1)) in human prostate fluid were:Fe 9.04 ±1.21,Zn 573 ± 35,Br 3.58 ± 0.59,Rb 1.10 ± 0.08,and Sr B 0.76.It was shown that the results of trace element analysis in the micro-samples(20 l L) are sufficiently representative for assessment of the Fe,Zn,Br,and Rb concentration in the prostate fluid.The facility for109 Cd radionuclide-induced EDXRF is comparatively compact and can be located in close proximity to the site of carrying out the massage procedure.The means of Zn and Rb concentration obtained for prostate fluid agree well with median of reported means.For the first time,the Fe and Br concentrations,as well as the upper limit of the Sr concentration,were determined in the human prostate fluid.
基金funded by a grant from Ferdowsi University of Mashhad
文摘Objective:To explore the serodiagnosis of hydatid cyst in human using different antigens of sheep(hydatid fluid,somatic and Lxcretory/secretory antigens of protoscolexi by ELISA and compares this result with commercial human ELISA kit.Methods:one hundred blood samples from patients with history of severe abdominal pain and cosinophilia were obtained.Ten serum samples were obtained from surgically and pathologically confirmed cystic echinococcosis patients from Mashhad university hospital as positive control and 5 serum samples from infant under one year old as negative control.Blood samples were centrifuged at 3000xg at 20℃for 15 min and sera were stored at-20℃.First,these samples were tested for the presence of antibody by commercial human ELISA.Then.ELISA was developed on microplates coated with hydatid fluid,Somatic and Excretory/secretory antigens of protoscolex of sheep.Results:The results of this study as analyzed by Kappa test showed that,hydatid fluid antigen could be used as a precise source of detection in indirect ELISA test.Conclusions:Hydatid fluid in comparison with Excretory-secretory and somatic antigens showed more compatibility agreement in kappa test which can be used for further studies in development of any ELISA test for diagnosis of human hydatidosis.
文摘Age-related NADH oxidase (arNOX = ENOX3) proteins are superoxide-generating cell surface oxidases that increase in activity with age beginning at about 30 y. A soluble and truncated exfoliated form of the activity is present in blood and other body fluids. The activity was purified to apparent homogeneity from human urine and resolved by 2-D gel electrophoresis into a series of 24 to 32 kDa components of low isoelectric point. The purified proteins were resistant both to N-terminal sequencing and trypsin cleavage. Cleavage with pepsin revealed peptides corresponding to the TM9 family of transmembrane proteins. Peptide antisera raised to all five members of the human TM9 family sequentially blocked the arNOX activity of human saliva and sera. The soluble truncated N-terminus of the human homolog TM9SF4 was expressed in bacteria. The recombinant protein was characterized biochemically and exhibited ar-NOX activity. The findings identify five arNOX isoforms each of which correspond to one of the five known TM9 family members. The exfoliated soluble arNOX forms are derived from the 24 to 32 kDa N-termini exposed to the cell’s exterior at the cell surface. Each of the shed forms contain putative functional motifs characteristic of ECTO-NOX (ENOX) proteins despite only minimal sequence identity. Our findings identify arNOX as having functional characteristics of ENOX proteins and the TM9 superfamily of proteins as the genetic origins of the five known arNOX isoforms present in human sera, plasma and other body fluids1.
文摘A three-dimensional computational fluid dynamics (CFD) simulation of the physiological pulsatile blood flow in the human aortic arch and its three branches has been conducted by using commercial software StarCD. The blood flow, of a peak Reynolds number of 3289 and a Womersley parameter of 16.44, was simulated in a rigid aorta geometry that was built by computer aided design (CAD) reconstruction method based on autopsy data of a female adult. The purpose of this work is to further the understanding of the complex nature of aorta flow, therefore it mainly focuses on analysis of the spatial and temporal distributions of velocities and wall shear stresses. The results, illustrated by 3D visualization pictures and 2D graphs of the primary velocity profiles, wall shear stress and pressure distributions, as well as the secondary flow patterns, are in good agreement with those of other experimental and computational works. The distributions of pressure and wall shear stress support the correlation between high and low shear stresses and pressures and the atherosclerotic lesions.
文摘The present theoretical assessment deals with the peristaltic-ciliary trans- port of a developing embryo within a fallopian tubal fluid in the human fallopian tube. A mathematical model of peristalsis-cilia induced flow of a linearly viscous fluid within a fallopian tubal fluid in a finite two-dimensional narrow tube is developed. The lubrica- tion approximation theory is used to solve the resulting partial differential equation. The expressions for axial and radial velocities, pressure gradient, stream function, volume flow rate, and time mean volume flow rate are derived. Numerical integration is performed for the appropriate residue time over the wavelength and the pressure difference over the wavelength. Moreover, the plots of axial velocity, the appropriate residue time over wavelength, the vector, the pressure difference over wavelength, and the streamlines are displayed and discussed for emerging parameters and constants. Salient features of the pumping characteristics and the trapping phenomenon are discussed in detail. Yhrther- more, a comparison between the peristaltic flow and the peristaltic-ciliary flow is made as the special case. Relevance of the current results to the transport of a developing embryo within a fallopian tubal fluid from ampulla to the intramural in the fallopian tube is also explored. It reveals the fact that cilia along with peristalsis helps to complete the required mitotic divisions while transporting the developing embryo within a fallopian tubal fluid in the human fallopian tube.
