Selection of the specific coalescent peptide of human CD59 by phage display techniques

To screen and identify the short peptides with specific binding activity to human CD59 and to design the short-peptide clamp against tumor escape, the phage display peptide library containing 12 peptides was used to select the highly expressed specific coalescent peptide of human CD59 in CHO cells. Positive phage clones obtained after 5 rounds of biopanning and detected with ELISA were obtained, in which 8 of them with high binding activity to human CD59 were sequenced. The 3 sequences thus obtained showed high homology with each and certain homology with sequence with human CD2 （PubMed 339HGAAENSLSPSS）, and all contained primary structure , of which this sequence may be the mimic confonnational epitope binding to human CD59. These results in the present study may be helpful to design the short-peptide clamp against the active sites of CD59 on tumor escape.

Providing universal access to Japanese humanities digital libraries:an approach to federated searching system using automatic metadata mapping

We present our approach for constructing a federated searching system for Japanese humanities digital libraries using automatic metadata mapping.We discuss some achievements in the ongoing research to construct a federated searching system. The goals of this system are(1) to perform metadata mapping automatically for Japanese heterogeneous humanities digital libraries and(2) to let users access multiple humanities digital libraries by using only one query input.The proposed automatic metadata mapping method produced the average mapping precision of 94.9%.We also address the metadata-related challenges facing Japanese humanities databases.

An improved yeast two-hybrid approach for detection of interacting proteins

Yeast two-hybrid approach is popularly used nowadays as an important technical method in the field of studying protein-protein interactions.Although yeast two-hybrid system is obviously advantageous in searching interacting proteins and setting up the network of proteins interaction,not all of proteins can use routine yeast two-hybrid method to search interacting proteins.Many important proteins,such as some nucleoprotein transcriptional factor,carry out the regular method and construct the bait-BD vector to screen the library containing AD vector.However,it usually results in failures because it contains the activate domain and can self-activate the reporter gene.In this study,we changed the research strategy,fused the bait gene(FOXA3)with the AD vector to screen the library containing BD vector,so that we constructed a two-hybrid library containing BD vector and can bypass the interference of self-activation.And we used this two-hybrid library to screen FOXA3,a hepatocyte nuclear factor,and found out an interacting protein:complement component C3.