A chemosensitivity test for ovarian cancer using tritiated thymidine incorporation assay was carried out. A dose-response relationship for cisplatin and potentiation of verapamil in increasing vincristine inhibition t...A chemosensitivity test for ovarian cancer using tritiated thymidine incorporation assay was carried out. A dose-response relationship for cisplatin and potentiation of verapamil in increasing vincristine inhibition to ovarian cancer were investigated. A 5- fold increase of cisplatin density converted the tumors which were initially resistance to standard-dose cisplatin Into drug-sensitive ones. Vera-pamil was found to be able to overcome vincristine-resistance of some tumors in vitro. These results suggest that using high dose cisplatin therapy or increasing local drug concentration by using other administration way, we could expect some ovarian cancers that had failed to standard dose cisplatin therapy to be effective. Combination of vincristine with verapamll may be helpful in treating some vincristineresistant cases.展开更多
The effects of antisense oligonucleotide to insulin-like growth factor 11 (IGFII) to induce apoptosis in human ovarian cancer cells were evaluated. Antiproliferation effects of antisense to IGFII in ovarian cancer AO ...The effects of antisense oligonucleotide to insulin-like growth factor 11 (IGFII) to induce apoptosis in human ovarian cancer cells were evaluated. Antiproliferation effects of antisense to IGFII in ovarian cancer AO cells were determined by 3H-thymidine incorporation. Apoptosis of the IGFll antisense-treated cells was quantitated by both nuclear condensation and flow cytometry after cells were stained with propidium iodide. IGFII antisense (4.5μM)treatment of 48 h maximally inhibited proliferation of AO cells. More than 25% of IGFII antisense-treated cells (4.5PM for 24 h) had undergone apoptosis, whereas less than 3% of the cells were apoptotic in either IGFII sense-treatedcells or untreated cells. Antisense oligonucleotide to IGFII significantly inhibited cell proliferation and induced apoptosis in human ovarian cancer AO cell. These data suggest that IGFII may be a potential target in treatment of ovarian cancer and antisense oligonucleotide to IGFⅡmay serve as a therapeutic approach.展开更多
Objective:To study explores the effect of HLEC on the secreted proteins of epithelial ovarian cancer(EOC)cells(SKOV3-PM4)with directional highly lymphatic metastasis.Methods:Supernatants of four groups of cultured cel...Objective:To study explores the effect of HLEC on the secreted proteins of epithelial ovarian cancer(EOC)cells(SKOV3-PM4)with directional highly lymphatic metastasis.Methods:Supernatants of four groups of cultured cells,namely,SKOV3(A),SKOV3+HLEC(B),SKOV3-PM4(C),SKOV3-PM4+HLEC(D),were collected,and their proteins were detected by antibody arrays and iTRAQ-2D-LC-MALDITOF/TOF/MS.Significantly differential proteins were further analyzed via bioinformatics and validated in human serums and cell media via ELISA.Results:Results of antibody arrays and mass spectrometry demonstrated that GRN and VEGFA were upregulated in group C(compared with group A),whereas IGFBP7 and SPARC were downregulated in group D(compared with group C).Comprehensive bioinformatics analysis results showed that IGFBP7 and VEGFA were closely linked to each other.Further validation with serums showed statistical significance in VEGFA and IGFBP7 levels among groups of patients with ovarian cancers,benign tumors,and control groups.Two proteins were upegulated in the first group.VEGFA in the control group was downregulated.For IGFBP,upregulation in the control group and down-regulation in the first group were also observed.Conclusion:The HLEC microenvironment is closely associated with directional metastasis to lymph nodes and with differential proteins including cell stromal proteins and adhesion factors.The upregulation of VEGFA and GRN and the downregulation of SPARC and IGFBP7 are closely associated with directional metastasis to lymph nodes in EOC cells.展开更多
文摘A chemosensitivity test for ovarian cancer using tritiated thymidine incorporation assay was carried out. A dose-response relationship for cisplatin and potentiation of verapamil in increasing vincristine inhibition to ovarian cancer were investigated. A 5- fold increase of cisplatin density converted the tumors which were initially resistance to standard-dose cisplatin Into drug-sensitive ones. Vera-pamil was found to be able to overcome vincristine-resistance of some tumors in vitro. These results suggest that using high dose cisplatin therapy or increasing local drug concentration by using other administration way, we could expect some ovarian cancers that had failed to standard dose cisplatin therapy to be effective. Combination of vincristine with verapamll may be helpful in treating some vincristineresistant cases.
文摘The effects of antisense oligonucleotide to insulin-like growth factor 11 (IGFII) to induce apoptosis in human ovarian cancer cells were evaluated. Antiproliferation effects of antisense to IGFII in ovarian cancer AO cells were determined by 3H-thymidine incorporation. Apoptosis of the IGFll antisense-treated cells was quantitated by both nuclear condensation and flow cytometry after cells were stained with propidium iodide. IGFII antisense (4.5μM)treatment of 48 h maximally inhibited proliferation of AO cells. More than 25% of IGFII antisense-treated cells (4.5PM for 24 h) had undergone apoptosis, whereas less than 3% of the cells were apoptotic in either IGFII sense-treatedcells or untreated cells. Antisense oligonucleotide to IGFII significantly inhibited cell proliferation and induced apoptosis in human ovarian cancer AO cell. These data suggest that IGFII may be a potential target in treatment of ovarian cancer and antisense oligonucleotide to IGFⅡmay serve as a therapeutic approach.
基金supported by the National Natural Science Foundation of China (Grant No. 81060218)Guangxi Natural Science Foundation (Grant No. 2012GXNSFAA053157)
文摘Objective:To study explores the effect of HLEC on the secreted proteins of epithelial ovarian cancer(EOC)cells(SKOV3-PM4)with directional highly lymphatic metastasis.Methods:Supernatants of four groups of cultured cells,namely,SKOV3(A),SKOV3+HLEC(B),SKOV3-PM4(C),SKOV3-PM4+HLEC(D),were collected,and their proteins were detected by antibody arrays and iTRAQ-2D-LC-MALDITOF/TOF/MS.Significantly differential proteins were further analyzed via bioinformatics and validated in human serums and cell media via ELISA.Results:Results of antibody arrays and mass spectrometry demonstrated that GRN and VEGFA were upregulated in group C(compared with group A),whereas IGFBP7 and SPARC were downregulated in group D(compared with group C).Comprehensive bioinformatics analysis results showed that IGFBP7 and VEGFA were closely linked to each other.Further validation with serums showed statistical significance in VEGFA and IGFBP7 levels among groups of patients with ovarian cancers,benign tumors,and control groups.Two proteins were upegulated in the first group.VEGFA in the control group was downregulated.For IGFBP,upregulation in the control group and down-regulation in the first group were also observed.Conclusion:The HLEC microenvironment is closely associated with directional metastasis to lymph nodes and with differential proteins including cell stromal proteins and adhesion factors.The upregulation of VEGFA and GRN and the downregulation of SPARC and IGFBP7 are closely associated with directional metastasis to lymph nodes in EOC cells.
