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Risk identification and safety assessment of human-computer interaction in integrated avionics based on STAMP
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作者 ZHAO Changxiao LI Hao +2 位作者 ZHANG Wei DAI Jun DONG Lei 《Journal of Systems Engineering and Electronics》 SCIE CSCD 2024年第3期689-706,共18页
To solve the problem of risk identification and quantitative assessment for human-computer interaction(HCI)in complex avionics systems,an HCI safety analysis framework based on system-theoretical process analysis(STPA... To solve the problem of risk identification and quantitative assessment for human-computer interaction(HCI)in complex avionics systems,an HCI safety analysis framework based on system-theoretical process analysis(STPA)and cognitive reliability and error analysis method(CREAM)is proposed.STPACREAM can identify unsafe control actions and find the causal path during the interaction of avionics systems and pilot with the help of formal verification tools automatically.The common performance conditions(CPC)of avionics systems in the aviation environment is established and a quantitative analysis of human failure is carried out.Taking the head-up display(HUD)system interaction process as an example,a case analysis is carried out,the layered safety control structure and formal model of the HUD interaction process are established.For the interactive behavior“Pilots approaching with HUD”,four unsafe control actions and35 causal scenarios are identified and the impact of common performance conditions at different levels on the pilot decision model are analyzed.The results show that HUD's HCI level gradually improves as the scores of CPC increase,and the quality of crew member cooperation and time sufficiency of the task is the key to its HCI.Through case analysis,it is shown that STPACREAM can quantitatively assess the hazards in HCI and identify the key factors that impact safety. 展开更多
关键词 AVIONICS human-computer interaction(HCI) safety assessment system-theoretic accident model and process human reliability analysis
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Identification and distribution of 13003 landslides in the northwest margin of Qinghai-Tibet Plateau based on human-computer interaction remote sensing interpretation
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作者 Wei Wang Yuan-dong Huang +8 位作者 Chong Xu Xiao-yi Shao Lei Li Li-ye Feng Hui-ran Gao Yu-long Cui Shuai Wu Zhi-qiang Yang Kai Ma 《China Geology》 CAS CSCD 2024年第2期171-187,共17页
The periphery of the Qinghai-Tibet Plateau is renowned for its susceptibility to landslides.However,the northwestern margin of this region,characterised by limited human activities and challenging transportation,remai... The periphery of the Qinghai-Tibet Plateau is renowned for its susceptibility to landslides.However,the northwestern margin of this region,characterised by limited human activities and challenging transportation,remains insufficiently explored concerning landslide occurrence and dispersion.With the planning and construction of the Xinjiang-Tibet Railway,a comprehensive investigation into disastrous landslides in this area is essential for effective disaster preparedness and mitigation strategies.By using the human-computer interaction interpretation approach,the authors established a landslide database encompassing 13003 landslides,collectively spanning an area of 3351.24 km^(2)(36°N-40°N,73°E-78°E).The database incorporates diverse topographical and environmental parameters,including regional elevation,slope angle,slope aspect,distance to faults,distance to roads,distance to rivers,annual precipitation,and stratum.The statistical characteristics of number and area of landslides,landslide number density(LND),and landslide area percentage(LAP)are analyzed.The authors found that a predominant concentration of landslide origins within high slope angle regions,with the highest incidence observed in intervals characterised by average slopes of 20°to 30°,maximum slope angle above 80°,along with orientations towards the north(N),northeast(NE),and southwest(SW).Additionally,elevations above 4.5 km,distance to rivers below 1 km,rainfall between 20-30 mm and 30-40 mm emerge as particularly susceptible to landslide development.The study area’s geological composition primarily comprises Mesozoic and Upper Paleozoic outcrops.Both fault and human engineering activities have different degrees of influence on landslide development.Furthermore,the significance of the landslide database,the relationship between landslide distribution and environmental factors,and the geometric and morphological characteristics of landslides are discussed.The landslide H/L ratios in the study area are mainly concentrated between 0.4 and 0.64.It means the landslides mobility in the region is relatively low,and the authors speculate that landslides in this region more possibly triggered by earthquakes or located in meizoseismal area. 展开更多
关键词 LANDSLIDES human-computer interaction interpretation Landslide database Spatial distribution Earthquake RAINFALL Human engineering activity Qinghai-Tibet Plateau Geological hazards survey engineering
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Design and Implementation of the Image Interactive System Based on Human-Computer Interaction
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作者 Shanwei Song Yuting Wu Fang Zhang 《Intelligent Information Management》 2010年第5期334-337,共4页
Based on the traditional Human-Computer Interaction method which is mainly touch input system, the way of capturing the movement of people by using cameras is proposed. This is a convenient technique which can provide... Based on the traditional Human-Computer Interaction method which is mainly touch input system, the way of capturing the movement of people by using cameras is proposed. This is a convenient technique which can provide users more experience. In the article, a new way of detecting moving things is given on the basis of development of the image processing technique. The system architecture decides that the communication should be used between two different applications. After considered, named pipe is selected from many ways of communication to make sure that video is keeping in step with the movement from the analysis of the people moving. According to a large amount of data and principal knowledge, thinking of the need of actual project, a detailed system design and realization is finished. The system consists of three important modules: detecting of the people's movement, information transition between applications and video showing in step with people's movement. The article introduces the idea of each module and technique. 展开更多
关键词 human-computer interaction IMAGE PROCESSING MOVING OBJECT Detection COMMUNICATION Response
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Isotope Tracking of Surface Water Groundwater Interaction in the Beninese Part of the Iullemeden Aquifer System
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作者 Houégnon Géraud Vinel Gbewezoun Samuel Yao Ganyaglo +4 位作者 Abdoukarim Alassane Samuel Boakye Dampare Gaya Salifou Orou Pete Alou Moussa Boukari Daouda Mama 《Journal of Water Resource and Protection》 CAS 2024年第7期489-501,共13页
The Kandi basin is located in northeast Benin (West Africa). This study is focused on the estimation of water fluxes exchanged between the river Niger (and its tributaries) and the transboundary Iullemeden Aquifer Sys... The Kandi basin is located in northeast Benin (West Africa). This study is focused on the estimation of water fluxes exchanged between the river Niger (and its tributaries) and the transboundary Iullemeden Aquifer System. In that framework, an innovative approach based on the application of the Bayesian Mixing Model (MixSIAR) analysis on water isotopes (oxygen-18, deuterium and tritium) was performed. Moreover, to assess the relevance of the model outputs, Pearson’s correlation and Principal Component Analysis (PCA) have been done. A complex relationship between surface water and groundwater has been found. Sixty percent (60%) of groundwater samples are made of more than 70% river water and rainwater;while 31.25% of surface water samples are made of about 84% groundwater. To safeguard sustainable water resources for the well-being of the local communities, surface water and groundwater must be managed as a unique component in the Kandi basin. 展开更多
关键词 BENIN West Africa Kandi basin Iullemeden Aquifer system Surface Water Groundwater interaction
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Effect of structural characteristics distribution on strength demand and ductility reduction factor of MDOF systems considering soil-structure interaction
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作者 Behnoud Ganjavi 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2012年第2期205-220,共16页
It is known that structural stiffness and strength distributions have an important role in the seismic response of buildings. The effect of using different code-specified lateral load patterns on the seismic performan... It is known that structural stiffness and strength distributions have an important role in the seismic response of buildings. The effect of using different code-specified lateral load patterns on the seismic performance of fixed-base buildings has been investigated by researchers during the past two decades. However, no investigation has yet been carried out for the case of soil-structure systems. In the present study, through intensive parametric analyses of 21,600 linear and nonlinear MDOF systems and considering five different shear strength and stiffness distribution patterns, including three code-specified patterns as well as uniform and concentric patterns subjected to a group of earthquakes recorded on alluvium and soft soils, the effect of structural characteristics distribution on the strength demand and ductility reduction factor of MDOF fixed-base and soil-structure systems are parametrically investigated. The results of this study show that depending on the level of inelasticity, soil flexibility and number of degrees-of-freedoms (DOFs), structural characteristics distribution can significantly affect the strength demand and ductility reduction factor of MDOF systems. It is also found that at high levels of inelasticity, the ductility reduction factor of low-rise MDOF soil-structure systems could be significantly less than that of fixed-base structures and the reduction is less pronounced as the number of stories increases. 展开更多
关键词 soil-structure interaction MDOF systems structural characteristic distribution inelastic behavior strength demand ductility reduction factor
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Interaction between cannabinoid and nucleotide systems as a new mechanism of signaling in retinal cell death
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作者 Hércules R. Freitas Ricardo A. M. Reis +1 位作者 Ana L. M. Ventura Guilherme R. Franca 《Neural Regeneration Research》 SCIE CAS CSCD 2019年第12期2093-2094,共2页
Nervous system emerges from complex signaling interactions where extrinsic (neurotransmitters and trophic factors, among others) and intrinsic factors (transcription factors) interplay in the developing tissue to cont... Nervous system emerges from complex signaling interactions where extrinsic (neurotransmitters and trophic factors, among others) and intrinsic factors (transcription factors) interplay in the developing tissue to control gene activity promoting chronic changes in cell genesis, migration, differentiation and death. The retinal microenvironment is regulated by a broad variety of chemicals, including endocannabinoids and nucleotides that modulate embryonic progenitor-neuron-Müller glia signaling in very early developing or pathophysiological conditions. 展开更多
关键词 interaction NUCLEOTIDE systems cell death
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Reform of Human-Computer Interaction Course based on Mobile Application Interaction Design
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作者 Siyang Lu Xiang Wei 《计算机教育》 2020年第12期122-125,共4页
With the popularity of new intelligent mobile devices in people’s lives,the development of mobile applications has paid increasing attention to the interactive experience of users.As the content of traditional Human-... With the popularity of new intelligent mobile devices in people’s lives,the development of mobile applications has paid increasing attention to the interactive experience of users.As the content of traditional Human-Computer Interaction(HCI)course and teaching material is out of date,it cannot meet the needs of mobile application interaction design and enterprises for students.Therefore,we need a new generation HCI course based on intelligent mobile devices to study the relationship between users and systems.The HCI course not only teaches students HCI theory and model,but also needs to cultivate students’interaction-oriented design practical ability.This paper proposes a set of HCI teaching material design and teaching methods for improving HCI class quality on mobile application interaction design,so as to make students more suitable for the employment requirements of enterprises. 展开更多
关键词 human-computer interaction software engineering mobile application interaction design
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A Simplified Method for Estimating the Initial Stiffness of Monopile-Soil Interaction Under Lateral Loads in Offshore Wind Turbine Systems
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作者 LI Xiao-juan DAI Guo-liang +2 位作者 ZHU Ming-xing WANG Li-yan LIU Hong-yuan 《China Ocean Engineering》 SCIE EI CSCD 2023年第1期165-174,共10页
The interface mechanical behavior of a monopile is an important component of the overall offshore wind turbine structure design.Understanding the soil-structure interaction,particularly the initial soil-structure stif... The interface mechanical behavior of a monopile is an important component of the overall offshore wind turbine structure design.Understanding the soil-structure interaction,particularly the initial soil-structure stiffness,has a significant impact on the study of natural frequency and dynamic response of the monopile.In this paper,a simplified method for estimating the interface mechanical behavior of monopiles under initial lateral loads is proposed.Depending on the principle of minimum potential energy and virtual work theory,the functions of soil reaction components at the interface of monopiles are derived;MATLAB programming has been used to simplify the functions of the initial stiffness by fitting a large number of examples;then the functions are validated against the field test data and FDM results.This method can modify the modulus of the subgrade reaction in the p-y curve method for the monopile-supported offshore wind turbine system. 展开更多
关键词 theoretical analysis pile−soil interaction interface mechanical behavior offshore wind turbine systems
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Interaction of Waves, Surface Currents, and Turbulence: the Application of Surface-Following Coordinate Systems
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作者 Jenkins Alastair D 《Journal of Ocean University of China》 SCIE CAS 2007年第4期319-331,共13页
Surface waves comprise an important aspect of the interaction between the atmosphere and the ocean, so a dynamically consistent framework for modelling atmosphere-ocean interaction must take account of surface waves, ... Surface waves comprise an important aspect of the interaction between the atmosphere and the ocean, so a dynamically consistent framework for modelling atmosphere-ocean interaction must take account of surface waves, either implicitly or explicitly. In order to calculate the effect of wind forcing on waves and currents, and vice versa, it is necessary to employ a consistent formula- tion of the energy and momentum balance within the airflow, wave field, and water column. It is very advantageous to apply sur- face-following coordinate systems, whereby the steep gradients in mean flow properties near the air-water interface in the cross-interface direction may be resolved over distances which are much smaller than the height of the waves themselves. We may account for the waves explicitly by employing a numerical spectral wave model, and applying a suitable theory of wave–mean flow interaction. If the mean flow is small compared with the wave phase speed, perturbation expansions of the hydrodynamic equations in a Lagrangian or generalized Lagrangian mean framework are useful: for stronger flows, such as for wind blowing over waves, the presence of critical levels where the mean flow velocity is equal to the wave phase speed necessitates the application of more general types of surface-following coordinate system. The interaction of the flow of air and water and associated differences in temperature and the concentration of various substances (such as gas species) gives rise to a complex boundary-layer structure at a wide range of vertical scales, from the sub-millimetre scales of gaseous diffusion, to several tens of metres for the turbulent Ekman layer. The bal- ance of momentum, heat, and mass is also affected significantly by breaking waves, which act to increase the effective area of the surface for mass transfer, and increase turbulent diffusive fluxes via the conversion of wave energy to turbulent kinetic energy. 展开更多
关键词 wind-wave-current interaction coordinate systems upper-ocean turbulence air-sea exchange of momentum heat andmass
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Coupling Effects of Depletion Interactions in Three-Sphere Systems with Different Size Ratio of Large- to Small-Sphere
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作者 Zhouan Zhou Dai Gang Changming Xiao 《Journal of Modern Physics》 2013年第6期761-765,共5页
The coupling effects of depletion interactions in three-sphere systems with different size ratio of large- to small-sphere are studied by Monte Carlosimulations in this paper. The numerical results show that this coup... The coupling effects of depletion interactions in three-sphere systems with different size ratio of large- to small-sphere are studied by Monte Carlosimulations in this paper. The numerical results show that this coupling effect is affected by the size ratio of large- to small-sphere: the larger the size ratio is, the larger the coupling effect will be. 展开更多
关键词 Coupling Effects of Depletion interactionS Three-Sphere systems SIZE RATIO
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THE INTERACTIONS OF SHOCK WAVES OF NONSTRICTLY HYPERBOLIC SYSTEMS
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作者 刘海亮 《Acta Mathematica Scientia》 SCIE CSCD 1992年第3期312-336,共25页
The interaction of shock waves is investigated for the following nonstrictly hyperbolic system: [GRAPHICS] The interaction of shock waves is complicated, with new types of shock waves, and new singula rities in the de... The interaction of shock waves is investigated for the following nonstrictly hyperbolic system: [GRAPHICS] The interaction of shock waves is complicated, with new types of shock waves, and new singula rities in the dependence of interaction on the relative positions of the three states separated by shock waves. Several ideas are introduced to helo organize and clarify the new phenomena. 展开更多
关键词 THE interactionS OF SHOCK WAVES OF NONSTRICTLY HYPERBOLIC systems der
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Dynamic constraint and objective generation approach for real-time train rescheduling model under human-computer interaction
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作者 Kai Liu Jianrui Miao +2 位作者 Zhengwen Liao Xiaojie Luan Lingyun Meng 《High-Speed Railway》 2023年第4期248-257,共10页
Real-time train rescheduling plays a vital role in railway transportation as it is crucial for maintaining punctuality and reliability in rail operations.In this paper,we propose a rescheduling model that incorporates... Real-time train rescheduling plays a vital role in railway transportation as it is crucial for maintaining punctuality and reliability in rail operations.In this paper,we propose a rescheduling model that incorporates constraints and objectives generated through human-computer interaction.This approach ensures that the model is aligned with practical requirements and daily operational tasks while facilitating iterative train rescheduling.The dispatcher’s empirical knowledge is integrated into the train rescheduling process using a human-computer interaction framework.We introduce six interfaces to dynamically construct constraints and objectives that capture human intentions.By summarizing rescheduling rules,we devise a rule-based conflict detection-resolution heuristic algorithm to effectively solve the formulated model.A series of numerical experiments are presented,demonstrating strong performance across the entire system.Furthermore,theflexibility of rescheduling is enhanced through secondary analysis-driven solutions derived from the outcomes of humancomputer interactions in the previous step.This proposed interaction method complements existing literature on rescheduling methods involving human-computer interactions.It serves as a tool to aid dispatchers in identifying more feasible solutions in accordance with their empirical rescheduling strategies. 展开更多
关键词 Real-time train rescheduling human-computer interaction Rule-based heuristic algorithm Secondary rescheduling
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THE INTRAMOLECULAR AROMATIC-RING STACKING INTERACTION OF MIXED LIGAND PALLADIUM(Ⅱ)COMPLEXES Ⅲ.STUDIES ON THE Pd^(2+)-A-UTP^(4-)SYSTEMS BY HNMR
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作者 Yu Qiu GONG Hong Liang SUN Department of Chemistry,Hangzhou University,Hangzhou,310028 《Chinese Chemical Letters》 SCIE CAS CSCD 1991年第7期593-594,共2页
The intramolecular aromatic-ring stacking interaction of mixed- ligand complex Pd(A)(UTP)^(2-)in the system pd^(2+)-A-UTP^(4-)has been determined by ~1HNMR,where A=1,10-phenanthroline(phen),2,2'-bipyridyl(bpy)and ... The intramolecular aromatic-ring stacking interaction of mixed- ligand complex Pd(A)(UTP)^(2-)in the system pd^(2+)-A-UTP^(4-)has been determined by ~1HNMR,where A=1,10-phenanthroline(phen),2,2'-bipyridyl(bpy)and DL- tryptophan(trp^-);UTP^(4-)=uridine 5-triphosphate.The result indicates that it is the partial stacking between the uracil ring of UTP^(4-)and the heterocyclic ring of A that makes H(5),H(6)and H(1')in the UTP^(4-)shift upfield signifi- cantly.Accordingly,the order of aromatic-ring interaction in the mixed- ligand complex has been obtained as follows:Pd(phen)(UTP)^(2-)(?)Pd(bpy)(UTP)^(2-) Pd(trp)(UTP)^(3-). 展开更多
关键词 systems BY HNMR THE INTRAMOLECULAR AROMATIC-RING STACKING interaction OF MIXED LIGAND PALLADIUM STUDIES ON THE Pd COMPLEXES A-UTP
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An investigation into natural vibrations of fluid-structure interaction systems subject to Sommerfeld radiation condition 被引量:2
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作者 Jing Tang Xing 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2008年第1期69-82,共14页
A fluid-structure interaction system subject to Sommerfeld's condition is defined as a Sommerfeld system which is divided into three categories: Fluid Sommerfeld (FS) System, Solid Sommerfeld (SS) System and Flu... A fluid-structure interaction system subject to Sommerfeld's condition is defined as a Sommerfeld system which is divided into three categories: Fluid Sommerfeld (FS) System, Solid Sommerfeld (SS) System and Fluid Solid Sommerfeld (FSS) System of which Sommerfeld conditions are imposed on a fluid boundary only, a solid boundary only and both fluid and solid boundaries, respectively. This paper follows the previous initial results claimed by simple examples to further mathematically investigate the natural vibrations of generalized Sommerfeld systems. A new parameter representing the speed of radiation wave for generalized 3-D problems with more complicated boundary conditions is introduced into the Sommerfeld condition which allows investigation of the natural vibrations of a Sommerfeld system involving both free surface and compressible waves. The mathematical demonstrations and selected examples confirm and reveal the natural behaviour of generalized Sommerfeld systems defined above. These generalized conclusions can be used in theoretical or engineering analysis of the vibrations of various Sommerfeld systems in engineering. 展开更多
关键词 Sommerfeld system Complex natural frequencies Fluid-structure interaction Complex energyflow identity Speed of radiation wave
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Interaction of HLA and TCP/IP subsystems in C^3I system
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作者 严海蓉 高武奇 +1 位作者 孙国基 钟联炯 《Journal of Pharmaceutical Analysis》 SCIE CAS 2008年第1期19-23,共5页
Aiming to the fact that the distribution modules of the C3I simulation system originally developed based on TCP/IP protocol has to be compatible with the present HLA (High Level of Architecture) rules, three methods f... Aiming to the fact that the distribution modules of the C3I simulation system originally developed based on TCP/IP protocol has to be compatible with the present HLA (High Level of Architecture) rules, three methods for implementing the interaction of HLA and TCP/IP subsystems are presented to make the original TCP/IP modules directly used in the HLA-based C3I system without redevelopment. The three methods are agent method, middleware method and gateway method. Moreover, the implementation of gateway method via VC++ as well as the key technology is discussed. 展开更多
关键词 HLA C3I simulation system interaction GATEWAY
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The Effect of the Interaction among Multi-Scale Systems and the Asymmetric an
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作者 徐祥德 陈联寿 《中国气象科学研究院年报》 1994年第0期11-20,共10页
THEEFFECTOFTHEINTERACTIONAMONGMULTI-SCALESYSTEMSANDTHEASYMMETRICDYNAMICANDTHERMODYNAMICSTRUCTUREOFTROPICALCY... THEEFFECTOFTHEINTERACTIONAMONGMULTI-SCALESYSTEMSANDTHEASYMMETRICDYNAMICANDTHERMODYNAMICSTRUCTUREOFTROPICALCYCLONEONITSTRACKXu... 展开更多
关键词 徐祥 The Effect of the interaction among Multi-Scale systems and the Asymmetric an
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A comprehensive study method for lifeline system interaction under seismic conditions 被引量:9
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作者 姚保华 谢礼立 火恩杰 《地震学报》 CSCD 北大核心 2004年第2期193-202,共10页
The mechanism of lifeline system interaction is analyzed and the method and its corresponding result for categorizing the lifeline system in terms of interaction are presented. Various methods for the different kind o... The mechanism of lifeline system interaction is analyzed and the method and its corresponding result for categorizing the lifeline system in terms of interaction are presented. Various methods for the different kind of interaction are reviewed in this paper. For the function interaction of the lifeline system, an all-round study method is recommended. A WebGIS based aided decision-making method for the post-earthquake restoration of multi-lifeline system is also presented. Meanwhile, some key-points for lifeline system interaction are discussed. 展开更多
关键词 生命线系统 相互作用 WEBGIS 地震
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Mode Interaction at a Triple Zero Point of O(2) symmetric Nonlinear Systems with Two Parameters
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作者 吴微 孟繁友 《Northeastern Mathematical Journal》 CSCD 2000年第1期10-20,共11页
We consider a triple zero point of nonlinear equations with O(2 symmetry, where the Jacobian has a zero eigenvalue of geometric multiplicity one and algebraic multiplicity three. We show that this triple zero point e... We consider a triple zero point of nonlinear equations with O(2 symmetry, where the Jacobian has a zero eigenvalue of geometric multiplicity one and algebraic multiplicity three. We show that this triple zero point exhibits a new bifurcation phenomenon, that is, a mode interaction of the following three paths: bifurcation points from steady states, steady states and rotating waves to standing waves, rotating waves and modulated rotating waves respectively. 展开更多
关键词 mode interaction BIFURCATION triple zero point O(2) symmetry nonlinear system
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Virtual brain surgery simulation system based on haptic interaction 被引量:3
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作者 张小瑞 Zhu Jiandong +4 位作者 Sun Wei Norman I.Badler Song Aiguo Niu Jianwei Liu Jia 《High Technology Letters》 EI CAS 2015年第2期185-191,共7页
To improve the accuracy and interactivity of soft tissue delormatlon simulation, a new plate spring model based on physics is proposed. The model is parameterized and thus can be adapted to simulate different organs. ... To improve the accuracy and interactivity of soft tissue delormatlon simulation, a new plate spring model based on physics is proposed. The model is parameterized and thus can be adapted to simulate different organs. Different soft tissues are modeled by changing the width, number of pieces, thickness, and length of a single plate spring. In this paper, the structural design, calcula- tion of soft tissue deformation and real-time feedback operations of our system are also introduced. To evaluate the feasibility of the system and validate the model, an experimental system of haptic in- teraction, in which users can use virtual hands to pull virtual brain tissues, is built using PHANTOM OMNI devices. Experimental results show that the proposed system is stable, accurate and promising for modeling instantaneous soft tissue deformation. 展开更多
关键词 haptic feedback human-computer interaction surgery simulation soft tissue deformation
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Analysis of Protein Interactions:Probing the Function of Proteins with Yeast Two-Hybrid System 被引量:1
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作者 唐巍 罗晓艳 Vanessa Samuls 《Forestry Studies in China》 CAS 2002年第1期49-57,共9页
The yeast two\|hybrid system is a molecular genetic approach for protein interaction and it is widely used to screen for proteins that interact with a protein of interest in recent years.This process includes,construc... The yeast two\|hybrid system is a molecular genetic approach for protein interaction and it is widely used to screen for proteins that interact with a protein of interest in recent years.This process includes,construction and testing of the bait plasmid,screening a plasmid library for interacting fusion protein,elimination of false positives and delection analysis of true positives.This procedure is designed to allow investigators to identify proteins and their encoding cDNAs that have a biologically significant interaction with a protein of interest.More and more studies have demonstrated that the two\|hybrid system is a powerful and sensitive technique for the identification of genes that code for proteins that interact in a biologically significant fashion with a protein of interest in higher plants.This method has been used to identify new interaction protein in many laboratories.The recently reported yeast tri\|brid system,should allow the investigation of more complex protein\|protein interactions.The aim of this review is to outline the recent progress made in protein interactions by using yeast two\|hybrid system. 展开更多
关键词 protein interaction two\|hybrid system YEAST transcription regulation
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