Slightly acidic electrolyzed water(SAEW)has proven to be an efficient and novel sanitizer in food and agriculture field.This study assessed the efficacy of SAEW(30 mg/L)at 40℃on the inactivation of foodbome pathogens...Slightly acidic electrolyzed water(SAEW)has proven to be an efficient and novel sanitizer in food and agriculture field.This study assessed the efficacy of SAEW(30 mg/L)at 40℃on the inactivation of foodbome pathogens and detachment of multi-resistant Staphylococcus aureus(MRSA)biofilm.Furthermore.the underlying mechanism of MRS A biofilm under heated SAEW at 40℃treatment on metabolic profiles was investigated.The results showed that the heated SAEW at 40℃significantly effectively against foodbome pathogens of 1.96-7.56(lg(CFU/g))reduction in pork,chicken,spinach,and lettuce.The heated SAEW at 40℃treatment significantly reduced MRS A biofilm cells by 2.41(lg(CFU/cm^(2))).The synergistic effect of SAEW treatment showed intense anti-biofilm activity in decreasing cell density and impairing biofilm cell membranes.Global metabolic response of MRSA biofilms,treated by SAEW at 40℃,revealed the alterations of intracellular metabolites,including amino acids,organic acid,fatty acid,and lipid.Moreover,signaling pathways involved in amino acid metabolism,energy metabolism,nucleotide synthesis,carbohydrate metabolites,and lipid biosynthesis were functionally disrupted by the SAEW at 40℃treatment.As per our knowledge,this is the first research to uncover the potential mechanism of heated SAEW treatment against MRSA biofilm on food contact surface.展开更多
End Polio Pakistan program still has to overcome many hurdles;unfortunately on 8th February2016 first polio case of the year has surfaced in Karachi.It seems that battle against polio demands little bit more convictio...End Polio Pakistan program still has to overcome many hurdles;unfortunately on 8th February2016 first polio case of the year has surfaced in Karachi.It seems that battle against polio demands little bit more conviction and motivation.WHO has set a goal of polio eradication in Pakistan till 2018,in order to evaluate the success of this target;polio eradication campaign in Pakistan has been analyzed in different perspectives.Our analysis indicated that major obstacles in eradication are low literacy rate,poor health infrastructure,lack of planning,natural disaster,economic crisis,counter insurgencies and almost no protection for polio health workers.WHO has allocated new funds to tackle this problem,now there is a need to spend this money more effectively with proper planning and honest deployment of funds.展开更多
基金supported by Brain Korea (BK)21 Plus Project (4299990913942)funded by the Korean Government,Koreathe Collabo Project funded by the Ministry of SMEs and Startups (C1016120-01-02)the National Research Foundation of Korea (NRF) (2018007551)。
文摘Slightly acidic electrolyzed water(SAEW)has proven to be an efficient and novel sanitizer in food and agriculture field.This study assessed the efficacy of SAEW(30 mg/L)at 40℃on the inactivation of foodbome pathogens and detachment of multi-resistant Staphylococcus aureus(MRSA)biofilm.Furthermore.the underlying mechanism of MRS A biofilm under heated SAEW at 40℃treatment on metabolic profiles was investigated.The results showed that the heated SAEW at 40℃significantly effectively against foodbome pathogens of 1.96-7.56(lg(CFU/g))reduction in pork,chicken,spinach,and lettuce.The heated SAEW at 40℃treatment significantly reduced MRS A biofilm cells by 2.41(lg(CFU/cm^(2))).The synergistic effect of SAEW treatment showed intense anti-biofilm activity in decreasing cell density and impairing biofilm cell membranes.Global metabolic response of MRSA biofilms,treated by SAEW at 40℃,revealed the alterations of intracellular metabolites,including amino acids,organic acid,fatty acid,and lipid.Moreover,signaling pathways involved in amino acid metabolism,energy metabolism,nucleotide synthesis,carbohydrate metabolites,and lipid biosynthesis were functionally disrupted by the SAEW at 40℃treatment.As per our knowledge,this is the first research to uncover the potential mechanism of heated SAEW treatment against MRSA biofilm on food contact surface.
文摘End Polio Pakistan program still has to overcome many hurdles;unfortunately on 8th February2016 first polio case of the year has surfaced in Karachi.It seems that battle against polio demands little bit more conviction and motivation.WHO has set a goal of polio eradication in Pakistan till 2018,in order to evaluate the success of this target;polio eradication campaign in Pakistan has been analyzed in different perspectives.Our analysis indicated that major obstacles in eradication are low literacy rate,poor health infrastructure,lack of planning,natural disaster,economic crisis,counter insurgencies and almost no protection for polio health workers.WHO has allocated new funds to tackle this problem,now there is a need to spend this money more effectively with proper planning and honest deployment of funds.
