Detection of Phaeocystis globosa using sandwich hybridization integrated with nuclease protection assay(NPA-SH)

Phaeocystis globosa Scherffel is one of the common harmful algae species in coastal waters of the southeastern China.In this study,sandwich hybridization integrated with nuclease protection assay(NPA-SH)was used to qualitatively and quantitatively detect P. globosa.Results showed that this method had good applicability and validity in analyzing the samples from laboratory cultures and from fields.The linear regression equation for P.globosa was obtained,and the lowest detection number of cells was 1.8×104 c...

Hybrid material integration in silicon photonic integrated circuits

Hybrid integration ofⅢ-Ⅴand ferroelectric materials is being broadly adopted to enhance functionalities in silicon photonic integrated circuits(PICs).Bonding and transfer printing have been the popular approaches for integration of III–V gain media with silicon PICs.Similar approaches are also being considered for ferroelectrics to enable larger RF modulation bandwidths,higher linearity,lower optical loss integrated optical modulators on chip.In this paper,we review existing integration strategies ofⅢ-Ⅴmaterials and present a route towards hybrid integration of bothⅢ-Ⅴand ferroelectrics on the same chip.We show that adiabatic transformation of the optical mode between hybrid ferroelectric and silicon sections enables efficient transfer of optical modal energies for maximum overlap of the optical mode with the ferroelectric media,similar to approaches adopted to maximize optical overlap with the gain section,thereby reducing lasing thresholds for hybridⅢ-Ⅴintegration with silicon PICs.Preliminary designs are presented to enable a foundry compatible hybrid integration route of diverse functionalities on silicon PICs.

Detection of Prorocentrum donghaiense using sandwich hybridization integrated with nuclease protection assay

Prorocentrum donghaiense is an important harmful algae bloom （HAB） causing creature in China＇s seas, and the conventional visual detection can not cope with long-term monitoring and highthroughput sampling projects. An assay for P. donghaiense with sandwich hybridization integrated with nuclease protection assay （NPA-SH） was established. Tests with mixed samples and spiked field ones confirmed its good specificity and sensitivity. The cell number of P. donghaiense correlated well with the optical density, and the regression equation is y=4× 10^- 6x＋ 0.694 9, in which x is the cell number, and y is the optical density, with r2=0.953 5. These results show that the NPA-SH method has good feasibility in the detection of P. donghaiense. Results of NPA-SH and microscopy are excellent for each sample. The NPA-SH method was a simple way in quantitative detection of P. donghaiense, and the whole process could be finished in about six hours, which provided a new approach in high-throughput sampling and long-term monitoring of P. donghaiense.

Quantifying Contribution of Recycled Moisture to Precipitation in Temperate Glacier Region,Southeastern Tibetan Plateau,China

Recycled moisture is an important indicator of the renewal capacity of regional water resources.Due to the existence of Yulong Snow Mountain,Lijiang in Yunnan Province,southeast of the Qinghai-Tibet Plateau,China,is the closest ocean glacier area to the equator in Eurasia.Daily precipitation samples were collected from 2017 to 2018 in Lijiang to quantify the effect of sub-cloud evaporation and recycled moisture on precipitation combined with the d-excess model during monsoon and non-monsoon periods.The results indicated that the d-excess values of precipitation fluctuated between–35.6‰and 16.0‰,with an arithmetic mean of 3.5‰.The local meteoric water line(LMWL)wasδD=7.91δ^(18)O+2.50,with a slope slightly lower than the global meteoric water line(GMWL).Subcloud evaporation was higher during the non-monsoon season than during the monsoon season.It tended to peak in March and was primarily influenced by the relative humidity.The source of the water vapour affected the proportion of recycled moisture.According to the results of the Hybrid Single-Particle Lagrangian Integrated Trajectory(HYSPLIT)model,the main sources of water vapour in Lijiang area during the monsoon period were the southwest and southeast monsoons.During the non-monsoon period,water vapour was transported by a southwesterly flow.The recycled moisture in Lijiang area between March and October 2017 was 10.62%.Large variations were observed between the monsoon and non-monsoon seasons,with values of 5.48%and 25.65%,respectively.These differences were primarily attributed to variations in the advection of water vapour.The recycled moisture has played a supplementary role in the precipitation of Lijiang area.

The 8×10 GHz Receiver Optical Subassembly Based on Silica Hybrid Integration Technology for Data Center Interconnection

An 8×10 GHz receiver optical sub-assembly （ROSA） consisting of an 8-channel arrayed waveguide grating （AWG） and an 8-channel PIN photodetector （PD） array is designed and fabricated based on silica hybrid integration technology. Multimode output waveguides in the silica AWG with 2% refractive index difference are used to obtain fiat-top spectra. The output waveguide facet is polished to 45° bevel to change the light propagation direction into the mesa-type PIN PD, which simplifies the packaging process. The experimentM results show that the single channel I dB bandwidth of AWG ranges from 2.12nm to 3.06nm, the ROSA responsivity ranges from 0.097 A/W to 0.158A/W, and the 3dB bandwidth is up to 11 GHz. It is promising to be applied in the eight-lane WDM transmission system in data center interconnection.

AN IMPROVED HYBRID BOUNDARY NODE METHOD IN TWO-DIMENSIONAL SOLIDS

The hybrid boundary node method （HBNM） is a promising method for solving boundary value problems with the hybrid displacement variational formulation and shape functions from the moving least squares（MLS） approximation. The main idea is to reduce the dimensionality of the former and keep the meshless advantage of the latter. Following its application in solving potential problems, it is further developed and numerically implemented for 2D solids in this paper. The rigid movement method is employed to solve the hyper-singular integrations. Numerical examples for some 2D solids have been given to show the characteristics. The computation results obtained by the present method are in excellent agreement with the analytical solution. The parameters that influence the performance of this method are studied through numerical examples.

Efficiency analysis of numerical integrations for finite element substructure in real-time hybrid simulation

Finite element（FE） is a powerful tool and has been applied by investigators to real-time hybrid simulations（RTHSs）. This study focuses on the computational efficiency, including the computational time and accuracy, of numerical integrations in solving FE numerical substructure in RTHSs. First, sparse matrix storage schemes are adopted to decrease the computational time of FE numerical substructure. In this way, the task execution time（TET） decreases such that the scale of the numerical substructure model increases. Subsequently, several commonly used explicit numerical integration algorithms, including the central difference method（CDM）, the Newmark explicit method, the Chang method and the Gui-λ method, are comprehensively compared to evaluate their computational time in solving FE numerical substructure. CDM is better than the other explicit integration algorithms when the damping matrix is diagonal, while the Gui-λ（λ = 4） method is advantageous when the damping matrix is non-diagonal. Finally, the effect of time delay on the computational accuracy of RTHSs is investigated by simulating structure-foundation systems. Simulation results show that the influences of time delay on the displacement response become obvious with the mass ratio increasing, and delay compensation methods may reduce the relative error of the displacement peak value to less than 5% even under the large time-step and large time delay.

Optical transmitter module with hybrid integration of DFB laser diode and proton-exchanged LiNbO_(3)modulator chip

In this work,a hybrid integrated optical transmitter module was designed and fabricated.A proton-exchanged Mach–Zehnder lithium niobate(LiNbO_(3))modulator chip was chosen to enhance the output extinction ratio.A fiber was used to adjust the rotation of the polarization direction caused by the optical isolator.The whole optical path structure,including the laser chip,lens,fiber,and modulator chip,was simulated to achieve high optical output efficiency.After a series of process improvements,a module with an output extinction ratio of 34 dB and a bandwidth of 20.5 GHz(from 2 GHz)was obtained.The optical output efficiency of the whole module reached approximately 21%.The link performance of the module was also measured.

Stability-constrained Two-stage Robust Optimization for Integrated Hydrogen Hybrid Energy System

In order to cope with the challenges brought by multiple uncertainties to integrated hydrogen hybrid energy systems,a stability-constrained two-stage robust optimization method considering small disturbance stability and characteristics of dynamic response is proposed in this paper.In the first operating stage,the charging/discharging state of the battery and the startstop state of the electrolyzer and fuel cell are determined.Then,power constraints for stabilizing ESSs power output is considered between the first and second stage optimization to improve the small disturbance stability of the robust operation plan.Then,the goal of minimizing the operation cost and determine a robust operation operating plan under the worst case is conducting in the second stage optimization.Through the small-signal model with time-delay effect,the eigenvalue analysis method is used to find the ESSs power range,and the small-signal stability of obtained robust operation plan can be enhanced.Finally,the effectiveness and superiority of the proposed method are proved by comparing with the traditional static robust optimization method.Impacts of uncertain parameters on economy and stability are also investigated in a typical example.

4 × 20 GHz silica-based AWG hybrid integrated receiver optical sub-assemblies

Both the 4 × 20 GHz coarse wavelength division multiplexing and LAN-WDM receiver optical sub-assemblies（ROSAs） were developed. The ROSA package was hybrid integrated with a planar lightwave circuit arrayed waveguide grating（AWG） with 2% refractive index difference and a four-channel top-illuminated positive-intrinsicnegative photodetector（PD） array. The output waveguides of the AWG were designed in a multimode structure to provide flat-top optical spectra, and their end facet was angle-polished to form a total internal reflection interface to realize vertical coupling with a PD array. The maximum responsivity of ROSA was about 0.4 A/W, and its 3 dB bandwidth of frequency response was up to 20 GHz for each transmission lane. The hybrid integrated ROSA would be a cost-effective and easy-assembling solution for 100 Gb E data center interconnections.

Dynamic response of axially moving Timoshenko beams: integral transform solution

The generalized integral transform technique （GITT） is used to find a semianalytical numerical solution for dynamic response of an axially moving Timoshenko beam with clamped-clamped and simply-supported boundary conditions, respectively. The implementation of GITT approach for analyzing the forced vibration equation eliminates the space variable and leads to systems of second-order ordinary differential equations （ODEs） in time. The MATHEMATICA built-in function, NDSolve, is used to numerically solve the resulting transformed ODE system. The good convergence behavior of the suggested eigenfunction expansions is demonstrated for calculating the transverse deflection and the angle of rotation of the beam cross-section. Moreover, parametric studies are performed to analyze the effects of the axially moving speed, the axial tension, and the amplitude of external distributed force on the vibration amplitude of axially moving Timoshenko beams.

112 Gbit/s transmitter optical subassembly based on hybrid integrated directly modulated lasers

Based on the hybrid integration technology, an ultra-compact and low cost transmitter optical subassembly module is proposed. Four directly modulated lasers are combined with a coarse wavelength division multiplexer operated at the O-band. The bandwidth for all channels is measured to be approximately 3 GHz. The 112 Gb∕s transmission is experimentally demonstrated for a 10 km standard single mode fiber（SSMF）, in which an optical isolator is used for avoiding the back-reflected and scattered light to improve the bit error rate（BER） performance. A low BER and clear eye opening are achieved for 10 km transmission.

Phosphor-free white light-emitting diodes

The multiple color-matching schemes that could improve the color rendering index for phosphor-free white LEDs are discussed. Then we review a few of the recent research directions for phosphor-free white LEDs, which include the development of monolithic GaN-based white LEDs and hybrid integrated GaN-based and A1GalnP-based white LEDs. These development paths will pave the way toward commercial application of phosphor-free white LEDs in the coming years.

Identifying water vapor sources of precipitation in forest and grassland in the north slope of the Tianshan Mountains,Central Asia

Identifying water vapor sources in the natural vegetation of the Tianshan Mountains is of significant importance for obtaining greater knowledge about the water cycle,forecasting water resource changes,and dealing with the adverse effects of climate change.In this study,we identified water vapor sources of precipitation and evaluated their effects on precipitation stable isotopes in the north slope of the Tianshan Mountains,China.By utilizing the temporal and spatial distributions of precipitation stable isotopes in the forest and grassland regions,Hybrid Single-Particle Lagrangian Integrated Trajectory(HYSPLIT)model,and isotope mass balance model,we obtained the following results.(1)The Eurasia,Black Sea,and Caspian Sea are the major sources of water vapor.(2)The contribution of surface evaporation to precipitation in forests is lower than that in the grasslands(except in spring),while the contribution of plant transpiration to precipitation in forests(5.35%)is higher than that in grasslands(3.79%)in summer.(3)The underlying surface and temperature are the main factors that affect the contribution of recycled water vapor to precipitation;meanwhile,the effects of water vapor sources of precipitation on precipitation stable isotopes are counteracted by other environmental factors.Overall,this work will prove beneficial in quantifying the effect of climate change on local water cycles.

Relationship Between Land Use Changes and the Production of Dust Sources in Kermanshah Province,Iran

Recognizing land use changes(LUC)and evaluating their relationship with producing dust sources are considered effective to manage the environment.Taking Kermanshah Province,Iran as study area,dusty days from 2008 to 2015 were selected and dust sources were identified applying thermal-infrared dust index(TDI),hybrid single-particle lagrangian integrated trajectory(HYSPLIT),false color composite(FCC)and true color composite(TCC)of MODerate resolution Imaging Spectroradiometer(MODIS)images.Afterwards,the land use change map was produced using Landsat images in 2000 and 2015.Then,the distribution and frequency of the sources in each land-use change class and important dust production areas were specified.Eventually,two non-parametric tests including Chisquare and Kruskal-Wallis were applied to examine the relationship between LUC and dust sources.Results indicated that the distribution of dust sources was not identical in the study area,and the sources were mainly generated in the areas where land-use change had occurred.In fact,different classes of LUC have different contributions to dust production,and the highest contribution refers to the deflation in gentle slope areas and lowlands where the rangeland has been converted into agriculture land.The findings from this study are useful to manage and control dust in the identified sources.

On the Nature of Caspian Clouds

Caspian clouds(CCs)are formed between the southern coast of the Caspian Sea and the Alborz Mountains.The purpose of this study is to identify characteristics of CCs using aerosol,cloud,and meteorological data from ModernEra Retrospective analysis for Research and Applications version 2(MERRA-2),Moderate Resolution Imaging Spectroradiometer(MODIS),and ECMWF Reanalysis version 5(ERA5)during 2000–2020.During this period,we identified and investigated 636 days with CCs.The results indicated that the frequency(%)of these clouds was higher in the summer than in other seasons because synoptic system activity varies between hot and cold periods.The hot season with the beginning of high-pressure subtropical Azores activity and the formation of a stable atmosphere in northern Iran leads to more frequent occurrence of CCs.These clouds are mainly the low-and middle-level clouds in the region,e.g.,stratus and altocumulus.CCs resulted in 13.9%of annual rainfall,and 55.9%and 18.7%of the summer and autumn rainfall,respectively,relative to total rainfall from all cloud types in the study region.In the multivariate regression analysis,CC precipitation exhibited a strong positive relationship with the cloud water path(CWP),cloud optical thickness(COT),and cloud effective radius(CER).A comparison of the mean and standard deviation of aerosol optical thickness(AOT)and aerosol index(AI)for CC and non-CC days did not show a significant difference.Examination of the synoptic patterns showed that the main factors in the formation of CCs are the specific environmental conditions of the region and the orographic lift of stable air masses.The Hybrid Single-Particle Lagrangian Integrated Trajectory(HYSPLIT)model indicated that the source of moisture for the formation of CCs was largely the Caspian Sea.

Analysis of Paths and Sources of Moisture for the South China Rainfall during the Presummer Rainy Season of 1979–2014

The paths and sources of moisture supplied to South China during two periods of the presummer rainy season （April-June） of 1979-2014, i.e., before and after the onset of the summer monsoon over the South China Sea （SCS）, are investigated by using the Hybrid Single-Particle Lagrangian Integrated Trajectory （HYSPLIT） model. During the premonsoon-onset period, the moisture transport trajectories are clustered into 6 groups, with four ocean-originating paths providing 83.9% and two continent-originating paths （originating over Lake Baikal and the Persian Gulf） con- tributing the remaining 16.1% of the total moisture. The two Pacific-originating paths, from the western Pacific Ocean and the East China Sea, combined account for about 46%, the SCS-originating path contributes about 24.3%, while the Bay of Bengal-originating path accounts for 13.6% of the total moisture over South China. The trajectories during the postmonsoon-onset period are clustered into 4 groups, with three southwesterly paths （from the Arabian Sea, the central Indian Ocean, and the western Indian Ocean, respectively） accounting for more than 76% and the sole Pacific-originating path accounting for 23.8% of the total moisture. The formation of the moisture transport tra-jectories is substantially affected by the topography, especially the Tibetan Plateau and the Indian and Indo-China Peninsulas. The SCS region contributes the most moisture during both periods （35.3% and 31.1%）. The Pacific Ocean is ranked second during the former period （about 21.0%） but its contribution is reduced to 5.0% during the lat-ter period, while the contribution from the Bay of Bengal and the Indian Ocean combined increases from 17.1% to 43.2%.

Dual-polarization multiplexing amorphous Si:H grating couplers for silicon photonic transmitters in the photonic BiCMOS backend of line

In this paper,we report on polarization combining two-dimensional grating couplers(2D GCs)on amorphous Si:H,fabricated in the backend of line of a photonic BiCMOS platform.The 2D GCs can be used as an interface of a hybrid silicon photonic coherent transmitter,which can be implemented on bulk Si wafers.The fabricated 2D GCs operate in the telecom C-band and show an experimental coupling efficiency of−5 dB with a wafer variation of±1.2 dB.Possibilities for efficiency enhancement and improved performance stability in future design generations are outlined and extension toward O-band devices is also investigated.

An 8-18 GHz broadband high power amplifier

An 8-18 GHz broadband high power amphtier （HPA） with a hybrid integrated circuit （HIC） is designed and fabricated. This HPA is achieved with the use of a 4-fingered micro-strip Lange coupler in a GaAs MMIC process. In order to decrease electromagnetic interference, a multilayer AIN material with good heat dissipation is adopted as the carrier of the power amplifier. When the input power is 25 dBm, the saturated power of the continuous wave （CW） outputted by the power amplifier is more than 39 dBm within the frequency range of8-13 GHz, while it is more than 38.6 dBm within other frequency ranges. We obtain the peak power output, 39.4 dBm, at the frequency of I 1.9 GHz. In the whole frequency band, the power-added efficiency is more than 18%. When the input power is 18 dBm, the small signal gain is 15.7 ± 0.7 dB. The dimensions of the HPA are 25 × 15 × 1.5 mm^3.

Evaluation of Two-center One- and Two-electron Integrals over Slater Type Orbitals

A formulation previously presented by the authors for coulomb integrals was generalized to other two-center integrals, except exchange integral. Within this frame, molecular integrals were expressed in terms of some new functions closely related to the well-known incomplete gamma functions and these functions recursively evaluated. Special issues arising in the case of hybrid integrals were addressed, and the results were compared with the ones found in the literature.