Neuroinflammation has been recognized to play a critical role in the pathogenesis of Alzheimer＇s disease （AD）, which is pathologically characterized by the accumulation of senile plaques containing activated microg...Neuroinflammation has been recognized to play a critical role in the pathogenesis of Alzheimer＇s disease （AD）, which is pathologically characterized by the accumulation of senile plaques containing activated microglia and amyloid β-peptides （Aβ）. In the present study, we examined the neuroprotective effects of hydrogen sulfide （H2S） on neuroinflammation in rats with Aβ1-40 hippocampal injection. We found that Aβ-induced rats exhibited a disorder of pyramidal cell layer arrangement, and a decrease of mean pyramidal cell number in the CA1 hippocampal region compared with those in sham operated rats. NaHS （a donor of H2S, 5.6 mg/kg/d, i.p.） treatment for 3 weeks rescued neuronal cell death significantly. Moreover, we found that H2S dramatically suppressed the release of TNF-α, IL-1β and IL-6 in the hippocampus. Consistently, both immunohistochemistry and Western blotting assays showed that H2S inhibited the upregulation of COX-2 and the activation of NF-κB in the hippocampus. In conclusion, our data indicate that H2S suppresses neuroinflammation via inhibition of the NF-κB activation pathway in the Aβ-induced rat model and has potential value for AD therapy.展开更多
Objective To establish the mitral regurgitation swine model and study the change of hydrogen sulfide(H2S)system in chronic heart failure model.Methods Miniature pigs were randomly divided into two groups,the control g...Objective To establish the mitral regurgitation swine model and study the change of hydrogen sulfide(H2S)system in chronic heart failure model.Methods Miniature pigs were randomly divided into two groups,the control group(n=6)and mitral regurgitation group(n=6).Chronic heart failure models were established by pulling mitral chordal through a small incision extracorporeal.展开更多
为保障工作人员在硫化氢(H_(2)S)泄漏工况下的生命安全,以东南部海域某高含硫油田开发工程为例,结合国内第一艘圆筒形浮式生产储油卸油装置(Floating Production Storage and Offloading,FPSO)的结构特点,设计一种适用于高含H_(2)S油气...为保障工作人员在硫化氢(H_(2)S)泄漏工况下的生命安全,以东南部海域某高含硫油田开发工程为例,结合国内第一艘圆筒形浮式生产储油卸油装置(Floating Production Storage and Offloading,FPSO)的结构特点,设计一种适用于高含H_(2)S油气田的FPSO应急空气呼吸系统。研究表明:该空气呼吸系统安全可靠、占地面积小、使用方便。研究成果可为H_(2)S防护设计提供一定参考。展开更多
With the aid of hydrogen permeating devices, the hydrogen permeation behaviors of X52 pipeline steel in NACE A solution with saturated H2S/CO2 were studied under the conditions of different ambient temperatures and pH...With the aid of hydrogen permeating devices, the hydrogen permeation behaviors of X52 pipeline steel in NACE A solution with saturated H2S/CO2 were studied under the conditions of different ambient temperatures and pH values, and the hydrogen permeation behaviors of X52 pipeline steel in weld seam zone were comparatively studied. The experimental results show that the hydrogen permeation coefficient value is directly proportional to the time required for reaching the saturation anode current and inversely proportional to the saturation anode current, and the hydrogen permeation coefficient is influenced by the corrosion scales; the temperature is directly proportional to the saturation anode current, and the hydrogen permeation coefficient is influenced by the temperature and corrosion scales, heat-affected zone and matrix zone in NACE A solution with saturated H2S/CO2 at normal temperature. The hydrogen permeation coefficient in weld seam zone is larger than that in heat-affected zone which is further larger than that in matrix zone.展开更多
OBJECTIVE To determine the functional role of hydrogen sulfide(H_2S) in protecting against mitochondrial dysfunction in heart failure through the inhibition of Ca^(2+)/calmodulin-dependent protein kinaseⅡ(Ca MKⅡ) us...OBJECTIVE To determine the functional role of hydrogen sulfide(H_2S) in protecting against mitochondrial dysfunction in heart failure through the inhibition of Ca^(2+)/calmodulin-dependent protein kinaseⅡ(Ca MKⅡ) using wild type and CSE knockout mouse models.METHODS Continuous subcutaneous injection isoprenaline(7.5 mg·kg^(-1) per day),once a day for 4 weeks to induce heart failure in male C57BL/6(6-8 weeks old) mice and CSE-/-mice.150 μmol·L^(-1) H_2O_2 was used to induce oxidative stress in H9c2 cells.Echocardiograph was used to detect cardiac parameters.H&E stain and Masson stain was to observation histopathological changes.Western blot was used to detect protein expression and activity.The si RNA was used to silence protein expression.HPLC was used to detect H_2S level.Biotin assay was used to detect the level of S-sulfhydration protein.RESULTS Treatment with S-propyl-L-cysteine(SPRC) or sodium hydrosulfide(Na HS),modulators of blood H_2S levels,attenuated the development of heart failure in animals,reduced lipid peroxidation,and preserved mitochondrial function.The inhibition Ca MKⅡ phosphorylation by SPRC and Na HS as demonstrated using both in vivo and in vitro models corresponded with the cardioprotective effects of these compounds.Interestingly,Ca MKⅡ activity was found to be elevated in CSE-/-mice as compared to wild type animals and the phosphorylation status of Ca MK Ⅱ appeared to relate to the severity of heart failure.Importantly,in wild type mice SPRC was found to promote S-sulfhydration of Ca MKⅡ leading to reduced activity of this protein however,in CSE-/-mice S-sulfhydration was abolished following SPRC treatment.CONCLUSION A novel mechanism depicting a role of S-sulfhydration in the regulation of Ca MKⅡ is presented.SPRC mediated S-sulfhydration of Ca MKⅡ was found to inhibit Ca MKⅡ activity and to preserve cardiovascular homeostasis.展开更多
Hydrogen sulfide is synthesized endogenously in mammals and has been shown to have both physiological and pathological functions. So far there has been little agreement as to the actual levels of endogenous sulfide un...Hydrogen sulfide is synthesized endogenously in mammals and has been shown to have both physiological and pathological functions. So far there has been little agreement as to the actual levels of endogenous sulfide under physiological or pathological conditions;this is partly due to the complexity involved in measuring free sulfides due to H2S volatility, oxidation, reactivity and the presence of bound labile sulfur in tissues. In this report we describe a method of measuring free tissue sulfides using a zinc sulfide precipitation and wash method. It is an indirect method that measures the sulfide difference between samples prepared at pH 9 and pH 6, assuming that at pH 9 free sulfides would be retained in solution, while at pH 6 free sulfides would volatilize during sample preparation. Using this approach we were able to measure appreciable amounts of free sulfides in mouse: lung, pancreas, liver and kidney at 0.036 + 0.006, 0.082 + 0.009, 0.215 + 0.016 and 0.323 + 0.031 nmole per mg of tissue respectively (n = 6).展开更多
基金supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions(No.Jx10131801095 to HongZhou)
文摘Neuroinflammation has been recognized to play a critical role in the pathogenesis of Alzheimer＇s disease （AD）, which is pathologically characterized by the accumulation of senile plaques containing activated microglia and amyloid β-peptides （Aβ）. In the present study, we examined the neuroprotective effects of hydrogen sulfide （H2S） on neuroinflammation in rats with Aβ1-40 hippocampal injection. We found that Aβ-induced rats exhibited a disorder of pyramidal cell layer arrangement, and a decrease of mean pyramidal cell number in the CA1 hippocampal region compared with those in sham operated rats. NaHS （a donor of H2S, 5.6 mg/kg/d, i.p.） treatment for 3 weeks rescued neuronal cell death significantly. Moreover, we found that H2S dramatically suppressed the release of TNF-α, IL-1β and IL-6 in the hippocampus. Consistently, both immunohistochemistry and Western blotting assays showed that H2S inhibited the upregulation of COX-2 and the activation of NF-κB in the hippocampus. In conclusion, our data indicate that H2S suppresses neuroinflammation via inhibition of the NF-κB activation pathway in the Aβ-induced rat model and has potential value for AD therapy.
文摘Objective To establish the mitral regurgitation swine model and study the change of hydrogen sulfide(H2S)system in chronic heart failure model.Methods Miniature pigs were randomly divided into two groups,the control group(n=6)and mitral regurgitation group(n=6).Chronic heart failure models were established by pulling mitral chordal through a small incision extracorporeal.
文摘为保障工作人员在硫化氢(H_(2)S)泄漏工况下的生命安全,以东南部海域某高含硫油田开发工程为例,结合国内第一艘圆筒形浮式生产储油卸油装置(Floating Production Storage and Offloading,FPSO)的结构特点,设计一种适用于高含H_(2)S油气田的FPSO应急空气呼吸系统。研究表明:该空气呼吸系统安全可靠、占地面积小、使用方便。研究成果可为H_(2)S防护设计提供一定参考。
基金supported by the Natural Science Foundation of Shandong China (No.2009ZRA05107)the Plan of Innovation Team of Ministry of Education China (No.IRT0759)
基金Funded by the Program for National Science Foundation for Distinguished Young Scholars (No.51125019)the National Natural Science Foundation of China (No.50904050)+2 种基金the Basic Projects of Sichuan Province of China(No.2011JY0106)Postdoctoral Science Foundation(No.20110490810)the Special Fund of China's Central Government for the Development of Local Colleges and Universities-the Project of National First-level Discipline in Oil and Gas Engineering
文摘With the aid of hydrogen permeating devices, the hydrogen permeation behaviors of X52 pipeline steel in NACE A solution with saturated H2S/CO2 were studied under the conditions of different ambient temperatures and pH values, and the hydrogen permeation behaviors of X52 pipeline steel in weld seam zone were comparatively studied. The experimental results show that the hydrogen permeation coefficient value is directly proportional to the time required for reaching the saturation anode current and inversely proportional to the saturation anode current, and the hydrogen permeation coefficient is influenced by the corrosion scales; the temperature is directly proportional to the saturation anode current, and the hydrogen permeation coefficient is influenced by the temperature and corrosion scales, heat-affected zone and matrix zone in NACE A solution with saturated H2S/CO2 at normal temperature. The hydrogen permeation coefficient in weld seam zone is larger than that in heat-affected zone which is further larger than that in matrix zone.
文摘OBJECTIVE To determine the functional role of hydrogen sulfide(H_2S) in protecting against mitochondrial dysfunction in heart failure through the inhibition of Ca^(2+)/calmodulin-dependent protein kinaseⅡ(Ca MKⅡ) using wild type and CSE knockout mouse models.METHODS Continuous subcutaneous injection isoprenaline(7.5 mg·kg^(-1) per day),once a day for 4 weeks to induce heart failure in male C57BL/6(6-8 weeks old) mice and CSE-/-mice.150 μmol·L^(-1) H_2O_2 was used to induce oxidative stress in H9c2 cells.Echocardiograph was used to detect cardiac parameters.H&E stain and Masson stain was to observation histopathological changes.Western blot was used to detect protein expression and activity.The si RNA was used to silence protein expression.HPLC was used to detect H_2S level.Biotin assay was used to detect the level of S-sulfhydration protein.RESULTS Treatment with S-propyl-L-cysteine(SPRC) or sodium hydrosulfide(Na HS),modulators of blood H_2S levels,attenuated the development of heart failure in animals,reduced lipid peroxidation,and preserved mitochondrial function.The inhibition Ca MKⅡ phosphorylation by SPRC and Na HS as demonstrated using both in vivo and in vitro models corresponded with the cardioprotective effects of these compounds.Interestingly,Ca MKⅡ activity was found to be elevated in CSE-/-mice as compared to wild type animals and the phosphorylation status of Ca MK Ⅱ appeared to relate to the severity of heart failure.Importantly,in wild type mice SPRC was found to promote S-sulfhydration of Ca MKⅡ leading to reduced activity of this protein however,in CSE-/-mice S-sulfhydration was abolished following SPRC treatment.CONCLUSION A novel mechanism depicting a role of S-sulfhydration in the regulation of Ca MKⅡ is presented.SPRC mediated S-sulfhydration of Ca MKⅡ was found to inhibit Ca MKⅡ activity and to preserve cardiovascular homeostasis.
文摘Hydrogen sulfide is synthesized endogenously in mammals and has been shown to have both physiological and pathological functions. So far there has been little agreement as to the actual levels of endogenous sulfide under physiological or pathological conditions;this is partly due to the complexity involved in measuring free sulfides due to H2S volatility, oxidation, reactivity and the presence of bound labile sulfur in tissues. In this report we describe a method of measuring free tissue sulfides using a zinc sulfide precipitation and wash method. It is an indirect method that measures the sulfide difference between samples prepared at pH 9 and pH 6, assuming that at pH 9 free sulfides would be retained in solution, while at pH 6 free sulfides would volatilize during sample preparation. Using this approach we were able to measure appreciable amounts of free sulfides in mouse: lung, pancreas, liver and kidney at 0.036 + 0.006, 0.082 + 0.009, 0.215 + 0.016 and 0.323 + 0.031 nmole per mg of tissue respectively (n = 6).
