Our knowledge about the food habits of sea snakes and how it is associated with their distribution has seen much development through its description across a number of species available through published literature ex...Our knowledge about the food habits of sea snakes and how it is associated with their distribution has seen much development through its description across a number of species available through published literature except for the key threatened species such asHydrophis semperi. This paper aims to describe the food habits ofH. semperi through gut content and stable isotope analyses. We also compared data with the Little File Snake,Acrochordus granulatus, sympatric withH. semperi.Recorded captures ofH. semperi suggest that the sea snake tends to occur in the littoral zones and the shallower portions of the limnetic zone. Gut content analysis ofH. semperi have shown that gobies and eels are primary prey items. Halfbeaks （Family Hemiramphidae） were recorded as one of the Lake Taal Sea Snake＇s prey items which is considered as a new prey record for sea snakes. These extracted gut contents are conifrmed to be the temporal food preference ofH. semperi given our detected stable isotope signatures. It appears thatA. granulatus and H. semperi share common prey items suggesting possible diet overlap and resource competition.This study reports the ifrst account of the endemic Lake Taal Sea Snake＇s distribution and food habits which poses implications towards its conservation as it occurs in a restricted ecosystem that has undergone considerable habitat alteration.展开更多
茚虫威和氯虫苯甲酰胺具有高效、持久的杀虫活性,对天敌昆虫及哺乳动物安全,在农作物害虫防治中被广泛应用。为明确茚虫威和氯虫苯甲酰胺在柞园及柞园周边农田使用对柞蚕生产的影响,采用喷施法测定2种杀虫剂对柞蚕的急性毒性、熏蒸毒性...茚虫威和氯虫苯甲酰胺具有高效、持久的杀虫活性,对天敌昆虫及哺乳动物安全,在农作物害虫防治中被广泛应用。为明确茚虫威和氯虫苯甲酰胺在柞园及柞园周边农田使用对柞蚕生产的影响,采用喷施法测定2种杀虫剂对柞蚕的急性毒性、熏蒸毒性和残毒期。结果表明,茚虫威和氯虫苯甲酰胺对柞蚕3龄幼虫的72 h LC50分别为0.465×10^-3、15.567×10^-3 mg/L,属于剧毒级;熏蒸毒性极低,全茧量和茧层量与对照差异不显著;2种药剂残毒期极长,均超过30 d。鉴于2种药剂对柞蚕具有很强的急性毒性和极长的残毒期,应当禁止在柞园内使用。展开更多
目的:采用基因沉默和慢病毒转染技术,沉默人肝癌细胞HepG2中特异蛋白3(specificity protein 3,Sp3)的表达,观察沉默Sp3基因后的肝细胞的增殖能力变化.方法:采用Sp3-siRNA慢病毒转染人肝癌细胞HepG2,通过免疫印迹法和PCR技术检测Sp3的表...目的:采用基因沉默和慢病毒转染技术,沉默人肝癌细胞HepG2中特异蛋白3(specificity protein 3,Sp3)的表达,观察沉默Sp3基因后的肝细胞的增殖能力变化.方法:采用Sp3-siRNA慢病毒转染人肝癌细胞HepG2,通过免疫印迹法和PCR技术检测Sp3的表达以验证转染效果,通过MTT检测细胞生长曲线和流式细胞技术测定细胞周期.结果:Western blot实验表明,实验组的Sp3表达量明显少于空白组和阴性对照组(0.37±0.08 vs 0.83±0.17,0.66±0.13,F=8.442,均P<0.05).RT-PCR也得到相同的结果(0.47±0.05 vs 0.74±0.08,0.70±0.16,F=7.322,均P<0.05).MTT实验结果显示,与空白对照和阴性对照组相比,实验组细胞在48、72和96h时的增殖明显受到抑制(0.28±0.18 vs 0.34±0.19,0.35±0.07,F=3.888;0.57±0.11 vs 0.84±0.05,0.74±0.08,F=12.721;0.72±18.1 vs 0.98±0.05,0.93±0.9,F=6.342,均P<0.05).流式细胞术结果显示实验组细胞主要分布在G1期.结论:RNAi沉默Sp3基因导致人肝癌HepG2细胞体外增殖能力下降.展开更多
基金financially supported by the Feasibility Study Grant from the Research Institute for Humanity and Naturethe Cooperative Research Grant from the Center for Ecological Research, Kyoto University, Japan
文摘Our knowledge about the food habits of sea snakes and how it is associated with their distribution has seen much development through its description across a number of species available through published literature except for the key threatened species such asHydrophis semperi. This paper aims to describe the food habits ofH. semperi through gut content and stable isotope analyses. We also compared data with the Little File Snake,Acrochordus granulatus, sympatric withH. semperi.Recorded captures ofH. semperi suggest that the sea snake tends to occur in the littoral zones and the shallower portions of the limnetic zone. Gut content analysis ofH. semperi have shown that gobies and eels are primary prey items. Halfbeaks （Family Hemiramphidae） were recorded as one of the Lake Taal Sea Snake＇s prey items which is considered as a new prey record for sea snakes. These extracted gut contents are conifrmed to be the temporal food preference ofH. semperi given our detected stable isotope signatures. It appears thatA. granulatus and H. semperi share common prey items suggesting possible diet overlap and resource competition.This study reports the ifrst account of the endemic Lake Taal Sea Snake＇s distribution and food habits which poses implications towards its conservation as it occurs in a restricted ecosystem that has undergone considerable habitat alteration.
文摘茚虫威和氯虫苯甲酰胺具有高效、持久的杀虫活性,对天敌昆虫及哺乳动物安全,在农作物害虫防治中被广泛应用。为明确茚虫威和氯虫苯甲酰胺在柞园及柞园周边农田使用对柞蚕生产的影响,采用喷施法测定2种杀虫剂对柞蚕的急性毒性、熏蒸毒性和残毒期。结果表明,茚虫威和氯虫苯甲酰胺对柞蚕3龄幼虫的72 h LC50分别为0.465×10^-3、15.567×10^-3 mg/L,属于剧毒级;熏蒸毒性极低,全茧量和茧层量与对照差异不显著;2种药剂残毒期极长,均超过30 d。鉴于2种药剂对柞蚕具有很强的急性毒性和极长的残毒期,应当禁止在柞园内使用。
文摘目的:采用基因沉默和慢病毒转染技术,沉默人肝癌细胞HepG2中特异蛋白3(specificity protein 3,Sp3)的表达,观察沉默Sp3基因后的肝细胞的增殖能力变化.方法:采用Sp3-siRNA慢病毒转染人肝癌细胞HepG2,通过免疫印迹法和PCR技术检测Sp3的表达以验证转染效果,通过MTT检测细胞生长曲线和流式细胞技术测定细胞周期.结果:Western blot实验表明,实验组的Sp3表达量明显少于空白组和阴性对照组(0.37±0.08 vs 0.83±0.17,0.66±0.13,F=8.442,均P<0.05).RT-PCR也得到相同的结果(0.47±0.05 vs 0.74±0.08,0.70±0.16,F=7.322,均P<0.05).MTT实验结果显示,与空白对照和阴性对照组相比,实验组细胞在48、72和96h时的增殖明显受到抑制(0.28±0.18 vs 0.34±0.19,0.35±0.07,F=3.888;0.57±0.11 vs 0.84±0.05,0.74±0.08,F=12.721;0.72±18.1 vs 0.98±0.05,0.93±0.9,F=6.342,均P<0.05).流式细胞术结果显示实验组细胞主要分布在G1期.结论:RNAi沉默Sp3基因导致人肝癌HepG2细胞体外增殖能力下降.
