The anti-hepatitis B virus(HBV)effects and its mechanisms of the ethanol extracts of Hypericum perforatum L.(EHP)in vitro were explored.HepG2 2.2.15 cells,a stable HBV-producing cell line,were cultured as the model sy...The anti-hepatitis B virus(HBV)effects and its mechanisms of the ethanol extracts of Hypericum perforatum L.(EHP)in vitro were explored.HepG2 2.2.15 cells,a stable HBV-producing cell line,were cultured as the model system to observe the anti-HBV effect.The viral antigens of cellular secretion,HBsAg and HBeAg,were determined by enzyme linked immunosorbent assay(ELISA).The quantity of HBV-DNA released in the supernatant was assayed by real-time PCR.In order to understand the mechanisms of the suppression of H...展开更多
To study the antiviral effect of Hypericum perforatum L. extract (HPE) on influenza A virus (IAV) (H1N1) in vitro and in vivo. Cytopathic effect (CPE) and neutral red (NR) dye uptake were used to examine the antiviral...To study the antiviral effect of Hypericum perforatum L. extract (HPE) on influenza A virus (IAV) (H1N1) in vitro and in vivo. Cytopathic effect (CPE) and neutral red (NR) dye uptake were used to examine the antiviral effect of HPE on Madin Darby Canine Kidney (MDCK) cells which were infected with IAV in vitro. HPE was effective against influenza A virus (IAV) in vitro, with a 50% effective concentration (EC50) of 40 μg/mL. The mean 50% cytotoxic concentration (CC50) in the MDCK used in these experiments was 1.5 mg/mL. Ribavirin was run in parallel with EC50 values of 5.0 μg/mL; the mean CC50 for ribavirin was 520 μg/mL. Oral gavage administrations of HPE or ribavirin to mice infected with the IAV were highly effective in preventing death, slowing the decline of arterial oxygen saturation, inhibiting lung consolidation and reducing lung virus titers. The minimum effective dose of HPE in these studies was 31.25 mg/kg/day, which was administered twice daily for 5 d beginning 4 h prior to virus exposure. Below a dosage of 2000 mg/kg/day, almost all treated mice survived, which suggests that HPE is of low toxicity. Ribavirin’s minimum effective dose was 40 mg/kg/day with the LD50 determined to be 200 mg/kg/day. Delay of the initiation of either HPE or ribavirin therapy, using approximately 1/3 LD50 dose each time, could still be protective as late as 48 h after exposure to the IAV. While both agents appeared to have similar efficacy against IAV infections, HPE was considered to be less toxic and may warrant further evaluation as a possible therapy for influenza.展开更多
Medicinal components of Hypericum perforatum L.plants varies widely due to fluctuations in growth environment and biotic and abiotic contamination during cultivation management.The quality of extracts or preparations ...Medicinal components of Hypericum perforatum L.plants varies widely due to fluctuations in growth environment and biotic and abiotic contamination during cultivation management.The quality of extracts or preparations is difficult to control because of the unstable raw materials.The aim of this study is to enhance the yield and medicinal component contents of H.perforatum by optimizing lighting factors under controlled environment.H.perforatum plants were hydroponically cultivated for 30 d under 3 levels of photosynthetic photon flux density(PPFD)with 200,300,and 400μmol/(m^(2)·s)using white LEDs(R:B ratio is the ratio of red light to blue light,R:B ratio of 0.9 and 1.8)and white plus red LED(R:B ratio of 2.7).The results showed that PPFD and LED spectrum had significant effects on the growth and accumulation of medicinal components of H.perforatum.Biomass accumulation of stem,leaf,and root increased linearly with the increase of PPFD under each LED spectrum.Fresh weights and dry weights of stem,leaf,and root were significantly higher under a PPFD of 400μmol/(m^(2)·s)with R:B ratio of 0.9 than those of 200μmol/(m^(2)·s),respectively.The relative growth rate and net photosynthetic rate showed linear relationships with PPFD under the same LED spectrum.Total hypericin content,total hyperforin content,and energy yield of hypericin increased with increasing PPFD.Total hypericin content and energy yield of hypericin of P400-L0.9 were 78%and 89%more than those of P400-L2.7,respectively.Total hyperforin content and energy yield of hyperforin of P400-L0.9 and P400-L2.7 were no significant differences.Based on energy efficiency,an R:B ratio of 0.9 of white LEDs with a PPFD of 400μmol/(m^(2)·s)was beneficial to improve medicinal component contents of hydroponic H.perforatum in plant factory with LED lighting.展开更多
Today, Hypericum perforatum L. is probably one of the best-characterized medicinal plants, and hyperforin is its best-characterized constituent. Extracts from H. perforatum are widely used as antidepressants;however, ...Today, Hypericum perforatum L. is probably one of the best-characterized medicinal plants, and hyperforin is its best-characterized constituent. Extracts from H. perforatum are widely used as antidepressants;however, less attention has been given to other properties of hyperforin, such as antitumor, fungicidal, antiviral and antibacterial action, or its possible use as a substance with immunomodulation properties. The present study summarizes results that describe the influence of hyperforin as an immunomodulation agent on phagocytosis and the breakdown of Escherichia coli by human polymorphonuclear neutrophils (PMNs). Hyperforin at 1 - 100 μg/mL concentrations was found to have a major influence on phagocytosis and the breakdown of E. coli by PMNs in vitro. A 100 μg/mL solution of hyperforin increased the uptake of non-opsonized E. coli almost 50-fold, and the uptake of IgG-opsonized E. coli more than threefold;on the other hand, the uptake of serum-opsonized bacteria was reduced to approximately 60% of that of the control. Hyperforin seems to bind to both PMNs and E. coli and acts like an opsonin. The elimination of remnants of IgG-opsonized E. coli from the PMNs was stimulated by hyperforin, while the elimination of remnants from non-op-so nized and serum-opsonized material was unaffected by the drug. Hyperforin exhibited clear immunomodulation ability as a phagocytosisstimulating agent. Hyperforin is probably inactive against human immunodeficiency virus (HIV) and most Gram-negative bacteria. However, it can protect acquired immunodeficiency syndrome (AIDS) patients and other immunocompromised patients by its antibacterial activity against Gram-positive bacteria and by enhancement of phagocytosis of Gram-positive and Gram-negative bacteria;some Gram-negative bacteria, such as Neisseria, are sensitive to hyperforin. Hyperforin has the ability to penetrate the blood-brain barrier (BBB) and blood-testis barrier (BTB) and is a valuable antibacterial agent against meningitis and gonorrhea. These properties of hyperforin are important for an antibiotic with immunomodulation activity in the struggle against the growing mortality in AIDS patients as a result of opportunistic bacteria, as recently shown by Bekondi et al. (2006, Int. J. Infect. Dis. 10, 387-395). It could also help to combat primary and opportunistic pathogens associated with meningitis in adults' relation to HIV serostatus.展开更多
文摘The anti-hepatitis B virus(HBV)effects and its mechanisms of the ethanol extracts of Hypericum perforatum L.(EHP)in vitro were explored.HepG2 2.2.15 cells,a stable HBV-producing cell line,were cultured as the model system to observe the anti-HBV effect.The viral antigens of cellular secretion,HBsAg and HBeAg,were determined by enzyme linked immunosorbent assay(ELISA).The quantity of HBV-DNA released in the supernatant was assayed by real-time PCR.In order to understand the mechanisms of the suppression of H...
基金One Hundred Person Project of The Chinese Academy of Sciences (2008-287) The Project of Basic Scientific Research Fund for Central Public-Welfare of Institute of Sciences (BRF070402).
文摘To study the antiviral effect of Hypericum perforatum L. extract (HPE) on influenza A virus (IAV) (H1N1) in vitro and in vivo. Cytopathic effect (CPE) and neutral red (NR) dye uptake were used to examine the antiviral effect of HPE on Madin Darby Canine Kidney (MDCK) cells which were infected with IAV in vitro. HPE was effective against influenza A virus (IAV) in vitro, with a 50% effective concentration (EC50) of 40 μg/mL. The mean 50% cytotoxic concentration (CC50) in the MDCK used in these experiments was 1.5 mg/mL. Ribavirin was run in parallel with EC50 values of 5.0 μg/mL; the mean CC50 for ribavirin was 520 μg/mL. Oral gavage administrations of HPE or ribavirin to mice infected with the IAV were highly effective in preventing death, slowing the decline of arterial oxygen saturation, inhibiting lung consolidation and reducing lung virus titers. The minimum effective dose of HPE in these studies was 31.25 mg/kg/day, which was administered twice daily for 5 d beginning 4 h prior to virus exposure. Below a dosage of 2000 mg/kg/day, almost all treated mice survived, which suggests that HPE is of low toxicity. Ribavirin’s minimum effective dose was 40 mg/kg/day with the LD50 determined to be 200 mg/kg/day. Delay of the initiation of either HPE or ribavirin therapy, using approximately 1/3 LD50 dose each time, could still be protective as late as 48 h after exposure to the IAV. While both agents appeared to have similar efficacy against IAV infections, HPE was considered to be less toxic and may warrant further evaluation as a possible therapy for influenza.
基金supported by China Agriculture Research System of MOF and MARA(CARS-21).
文摘Medicinal components of Hypericum perforatum L.plants varies widely due to fluctuations in growth environment and biotic and abiotic contamination during cultivation management.The quality of extracts or preparations is difficult to control because of the unstable raw materials.The aim of this study is to enhance the yield and medicinal component contents of H.perforatum by optimizing lighting factors under controlled environment.H.perforatum plants were hydroponically cultivated for 30 d under 3 levels of photosynthetic photon flux density(PPFD)with 200,300,and 400μmol/(m^(2)·s)using white LEDs(R:B ratio is the ratio of red light to blue light,R:B ratio of 0.9 and 1.8)and white plus red LED(R:B ratio of 2.7).The results showed that PPFD and LED spectrum had significant effects on the growth and accumulation of medicinal components of H.perforatum.Biomass accumulation of stem,leaf,and root increased linearly with the increase of PPFD under each LED spectrum.Fresh weights and dry weights of stem,leaf,and root were significantly higher under a PPFD of 400μmol/(m^(2)·s)with R:B ratio of 0.9 than those of 200μmol/(m^(2)·s),respectively.The relative growth rate and net photosynthetic rate showed linear relationships with PPFD under the same LED spectrum.Total hypericin content,total hyperforin content,and energy yield of hypericin increased with increasing PPFD.Total hypericin content and energy yield of hypericin of P400-L0.9 were 78%and 89%more than those of P400-L2.7,respectively.Total hyperforin content and energy yield of hyperforin of P400-L0.9 and P400-L2.7 were no significant differences.Based on energy efficiency,an R:B ratio of 0.9 of white LEDs with a PPFD of 400μmol/(m^(2)·s)was beneficial to improve medicinal component contents of hydroponic H.perforatum in plant factory with LED lighting.
文摘Today, Hypericum perforatum L. is probably one of the best-characterized medicinal plants, and hyperforin is its best-characterized constituent. Extracts from H. perforatum are widely used as antidepressants;however, less attention has been given to other properties of hyperforin, such as antitumor, fungicidal, antiviral and antibacterial action, or its possible use as a substance with immunomodulation properties. The present study summarizes results that describe the influence of hyperforin as an immunomodulation agent on phagocytosis and the breakdown of Escherichia coli by human polymorphonuclear neutrophils (PMNs). Hyperforin at 1 - 100 μg/mL concentrations was found to have a major influence on phagocytosis and the breakdown of E. coli by PMNs in vitro. A 100 μg/mL solution of hyperforin increased the uptake of non-opsonized E. coli almost 50-fold, and the uptake of IgG-opsonized E. coli more than threefold;on the other hand, the uptake of serum-opsonized bacteria was reduced to approximately 60% of that of the control. Hyperforin seems to bind to both PMNs and E. coli and acts like an opsonin. The elimination of remnants of IgG-opsonized E. coli from the PMNs was stimulated by hyperforin, while the elimination of remnants from non-op-so nized and serum-opsonized material was unaffected by the drug. Hyperforin exhibited clear immunomodulation ability as a phagocytosisstimulating agent. Hyperforin is probably inactive against human immunodeficiency virus (HIV) and most Gram-negative bacteria. However, it can protect acquired immunodeficiency syndrome (AIDS) patients and other immunocompromised patients by its antibacterial activity against Gram-positive bacteria and by enhancement of phagocytosis of Gram-positive and Gram-negative bacteria;some Gram-negative bacteria, such as Neisseria, are sensitive to hyperforin. Hyperforin has the ability to penetrate the blood-brain barrier (BBB) and blood-testis barrier (BTB) and is a valuable antibacterial agent against meningitis and gonorrhea. These properties of hyperforin are important for an antibiotic with immunomodulation activity in the struggle against the growing mortality in AIDS patients as a result of opportunistic bacteria, as recently shown by Bekondi et al. (2006, Int. J. Infect. Dis. 10, 387-395). It could also help to combat primary and opportunistic pathogens associated with meningitis in adults' relation to HIV serostatus.
