Aim: To determine the predictive value of the hypo-osmotic swelling (HOS) test to identify viable, non-motile sperm. Methods: Semen samples from 20 men with severe asthenozoospermia underwent traditional seminal analy...Aim: To determine the predictive value of the hypo-osmotic swelling (HOS) test to identify viable, non-motile sperm. Methods: Semen samples from 20 men with severe asthenozoospermia underwent traditional seminal analysis, eosin-nigrosin (EN) staining and the HOS test. A further EN stain was then performed on a HOS pre-treated aliquot and a total of 2000 further sperm examined. Results: The median sperm density was 5.1 million/mL (IQR 4.3-13.1) and the median motility was 3.0 % (IQR 0-7). Seven samples showed complete asthenozoospermia. Initial EN staining showed 59 % viability (range 48-69) despite the poor standard parameters and 47 % (range 33-61) in the complete asthenozoospermia subgroup. The HOS test showed 49.9 % reacted overall (range 40-59) and 41.7 % (range 22-61) in the complete asthenozoospermia subgroup. The combined HOS/EN stain showed the positive predictive value of the HOS test to identify viable sperm was 84.2 % overall and 79.7 % in the complete asthenozoospermia subgroup. Conclusion: The HOS test can effectively predict sperm viability in patients with severe and complete asthenozoospermia.展开更多
共检测110例男性不育患者的精液标本,确证精子尾部低渗膨胀(HOS)试验与精子活动率有较显著的正相关(r=0.273,n=102,P<0.01);与精子膜表面麦芽凝集素(WGA)受体亦成正相关(r=0.251,n=72,P<0.05);但与精子膜表面附着的抗精抗体免疫微...共检测110例男性不育患者的精液标本,确证精子尾部低渗膨胀(HOS)试验与精子活动率有较显著的正相关(r=0.273,n=102,P<0.01);与精子膜表面麦芽凝集素(WGA)受体亦成正相关(r=0.251,n=72,P<0.05);但与精子膜表面附着的抗精抗体免疫微珠(IB)试验无关(r=0.027,n=62,P>0.05);与精液有无支原体感染无关(t=0.543,df=32,P>0.05);精液中圆细胞数量对 HOS 试验无影响(F=0.413,方差自由度为f_1=3,f_2=100,P>0.05)。故认为以上试验互不能取代,只能互相参考。我们用简便而可靠的铁苏木精染色法改进了 HOS 试验技术,以便于这种确有实用价值的精子功能检测技术在临床上推广。展开更多
文摘Aim: To determine the predictive value of the hypo-osmotic swelling (HOS) test to identify viable, non-motile sperm. Methods: Semen samples from 20 men with severe asthenozoospermia underwent traditional seminal analysis, eosin-nigrosin (EN) staining and the HOS test. A further EN stain was then performed on a HOS pre-treated aliquot and a total of 2000 further sperm examined. Results: The median sperm density was 5.1 million/mL (IQR 4.3-13.1) and the median motility was 3.0 % (IQR 0-7). Seven samples showed complete asthenozoospermia. Initial EN staining showed 59 % viability (range 48-69) despite the poor standard parameters and 47 % (range 33-61) in the complete asthenozoospermia subgroup. The HOS test showed 49.9 % reacted overall (range 40-59) and 41.7 % (range 22-61) in the complete asthenozoospermia subgroup. The combined HOS/EN stain showed the positive predictive value of the HOS test to identify viable sperm was 84.2 % overall and 79.7 % in the complete asthenozoospermia subgroup. Conclusion: The HOS test can effectively predict sperm viability in patients with severe and complete asthenozoospermia.
文摘共检测110例男性不育患者的精液标本,确证精子尾部低渗膨胀(HOS)试验与精子活动率有较显著的正相关(r=0.273,n=102,P<0.01);与精子膜表面麦芽凝集素(WGA)受体亦成正相关(r=0.251,n=72,P<0.05);但与精子膜表面附着的抗精抗体免疫微珠(IB)试验无关(r=0.027,n=62,P>0.05);与精液有无支原体感染无关(t=0.543,df=32,P>0.05);精液中圆细胞数量对 HOS 试验无影响(F=0.413,方差自由度为f_1=3,f_2=100,P>0.05)。故认为以上试验互不能取代,只能互相参考。我们用简便而可靠的铁苏木精染色法改进了 HOS 试验技术,以便于这种确有实用价值的精子功能检测技术在临床上推广。
