Miniature inverted-repeat transposable elements(MITEs)are a group of DNA transposable element(TE)which preferentially distributed with gene associated regions.Tens of MITEs families have been revealed in Brassica napu...Miniature inverted-repeat transposable elements(MITEs)are a group of DNA transposable element(TE)which preferentially distributed with gene associated regions.Tens of MITEs families have been revealed in Brassica napus genome,they scatter across the genome with tens of thousands copies and produce polymorphisms both intra-and inter-species.Our previous studies revealed a Tourist-like MITE,Monkey King,associated with vernalization requirement of B.napus,however there are still few studies reveal MITE association with agricultural traits in B.napus.In the present study,80 polymorphic markers were developed from 55 MITEs,and used to evaluate genetic diversity in a panel of B.napus accessions consisting of 101 natural and 25 synthetic genotypes.Five agricultural traits,oil content,glucosinolate content,erucic acid content,weight of thousand seeds(WTS)and plant height,were investigated across 3-years field experiments,in addition,two traits,hypocotyl length and root length,were evaluated at the 4-leaf stage in the laboratory.Correlations between the MITE-based markers and seven traits were analyzed,finally,10 polymorphic markers produced by 6 pairs of MITE specific primers were revealed relatively high correlation with 5 traits.Two polymorphic markers were anchored with two candidate genes,BnaA02g13530D and BnaA08g20010D,respectively,which may contribute to glucosinolate content and WTS.This research may contribute to genetic improvement through utilization of MITE-induced polymorphisms in Brassica species.展开更多
Supplemental lighting can be applied in the greenhouse to obtain high-quality seedlings when the solar daily light integral(DLI)is insufficient.However,there is no optimal strategy for the supplementary light provided...Supplemental lighting can be applied in the greenhouse to obtain high-quality seedlings when the solar daily light integral(DLI)is insufficient.However,there is no optimal strategy for the supplementary light provided by white and blue light-emitting diodes(LEDs)with the same DLI in cucumber(Cucumis sativus L.)seedling production grown in the greenhouse in early spring.The objective of the study was to determine changes in morphology,photosynthesis,growth,and physiological characteristics in greenhouse-grown cucumber seedlings(cv.Tianjiao No.5)depending on different supplementary fractions(28.5%,33.5%,38.5%,43.5%,and 48.5%)of blue light(B)under constant DLI provided by combinations of white(B28.5%included)and blue LEDs,and cucumber seedlings were grown with sunlight only were set as the control.The results documented that supplementary light resulted in compact and robust greenhouse-grown cucumber seedlings with higher chlorophyll content and net photosynthetic rate compared to those grown without supplementary light.The plant height and hypocotyl length of cucumber seedlings decreased quadratically with an increase of blue light fractions provided by combinations of white and blue LEDs.Additionally,the leaf area and stem diameter of cucumber seedlings increased first and a decreased trend was observed subsequently with the increasing fraction of blue light in a quadratic function.Similar trends were found in root architecture(e.g.,root length,root surface area,and root volume)and root activity of cucumber seedlings;however,no significant differences were exhibited as blue light fraction increased from 38.5%to 43.5%provided by supplementary light.Stem firmness and cellulose content increased by 26.2%and 23.4%,respectively,as 15%blue light was added to white LEDs.In conclusion,the 43.5%blue light created by supplementary broad-spectrum white and blue LEDs resulted in compact and stoutest cucumber seedlings along with well-developed root system and higher stem firmness,thus improving the mechanical strength of the greenhouse-grown cucumber seedlings for transplanting.展开更多
基金supported by National Natural Science Foundation of China(No.31501341)Outstanding Youth Foundation of Henan Academy of Agricultural Sciences(No.2016YQ29).
文摘Miniature inverted-repeat transposable elements(MITEs)are a group of DNA transposable element(TE)which preferentially distributed with gene associated regions.Tens of MITEs families have been revealed in Brassica napus genome,they scatter across the genome with tens of thousands copies and produce polymorphisms both intra-and inter-species.Our previous studies revealed a Tourist-like MITE,Monkey King,associated with vernalization requirement of B.napus,however there are still few studies reveal MITE association with agricultural traits in B.napus.In the present study,80 polymorphic markers were developed from 55 MITEs,and used to evaluate genetic diversity in a panel of B.napus accessions consisting of 101 natural and 25 synthetic genotypes.Five agricultural traits,oil content,glucosinolate content,erucic acid content,weight of thousand seeds(WTS)and plant height,were investigated across 3-years field experiments,in addition,two traits,hypocotyl length and root length,were evaluated at the 4-leaf stage in the laboratory.Correlations between the MITE-based markers and seven traits were analyzed,finally,10 polymorphic markers produced by 6 pairs of MITE specific primers were revealed relatively high correlation with 5 traits.Two polymorphic markers were anchored with two candidate genes,BnaA02g13530D and BnaA08g20010D,respectively,which may contribute to glucosinolate content and WTS.This research may contribute to genetic improvement through utilization of MITE-induced polymorphisms in Brassica species.
基金supported by the National Key Research and Development Program of China(2019YFD1001901)the Modern Agricultural Industrial Technology System of Shandong Province(SDAITG05G06)+1 种基金the Natural Science Foundation of Shandong Province(ZR2021QC174)the Experimental Technology Research Program of Qingdao Agricultural University(SYJS202117).
文摘Supplemental lighting can be applied in the greenhouse to obtain high-quality seedlings when the solar daily light integral(DLI)is insufficient.However,there is no optimal strategy for the supplementary light provided by white and blue light-emitting diodes(LEDs)with the same DLI in cucumber(Cucumis sativus L.)seedling production grown in the greenhouse in early spring.The objective of the study was to determine changes in morphology,photosynthesis,growth,and physiological characteristics in greenhouse-grown cucumber seedlings(cv.Tianjiao No.5)depending on different supplementary fractions(28.5%,33.5%,38.5%,43.5%,and 48.5%)of blue light(B)under constant DLI provided by combinations of white(B28.5%included)and blue LEDs,and cucumber seedlings were grown with sunlight only were set as the control.The results documented that supplementary light resulted in compact and robust greenhouse-grown cucumber seedlings with higher chlorophyll content and net photosynthetic rate compared to those grown without supplementary light.The plant height and hypocotyl length of cucumber seedlings decreased quadratically with an increase of blue light fractions provided by combinations of white and blue LEDs.Additionally,the leaf area and stem diameter of cucumber seedlings increased first and a decreased trend was observed subsequently with the increasing fraction of blue light in a quadratic function.Similar trends were found in root architecture(e.g.,root length,root surface area,and root volume)and root activity of cucumber seedlings;however,no significant differences were exhibited as blue light fraction increased from 38.5%to 43.5%provided by supplementary light.Stem firmness and cellulose content increased by 26.2%and 23.4%,respectively,as 15%blue light was added to white LEDs.In conclusion,the 43.5%blue light created by supplementary broad-spectrum white and blue LEDs resulted in compact and stoutest cucumber seedlings along with well-developed root system and higher stem firmness,thus improving the mechanical strength of the greenhouse-grown cucumber seedlings for transplanting.
