Ice structuring proteins(ISPs)isolated from the cold-acclimated plants have a great potential in improving the quality of frozen foods.The purification of ISP complexes from winter wheat was achieved using an aqueous ...Ice structuring proteins(ISPs)isolated from the cold-acclimated plants have a great potential in improving the quality of frozen foods.The purification of ISP complexes from winter wheat was achieved using an aqueous two-phase system of Triton X-114.The highly reactive phenols were removed,and the ISP complexes remained in the aqueous phase after phase separation.The ISP complexes treated by this procedure retained higher thermal hysteresis activity than those treated by ammonium sulfate method.The phase separation technique provides a simple and mild way for removing phenols from ISP complexes.展开更多
基金supported by the Natural Science Foundation of Jiangnan University(2008LYY 024).
文摘Ice structuring proteins(ISPs)isolated from the cold-acclimated plants have a great potential in improving the quality of frozen foods.The purification of ISP complexes from winter wheat was achieved using an aqueous two-phase system of Triton X-114.The highly reactive phenols were removed,and the ISP complexes remained in the aqueous phase after phase separation.The ISP complexes treated by this procedure retained higher thermal hysteresis activity than those treated by ammonium sulfate method.The phase separation technique provides a simple and mild way for removing phenols from ISP complexes.